Mutagenetix > Incidental Mutation

Incidental Mutation 'R4026:Adgrg7'

313003

Institutional Source

Beutler Lab

Gene Symbol

Adgrg7

Ensembl Gene

ENSMUSG00000022755

Gene Name

adhesion G protein-coupled receptor G7

Synonyms

9130020O16Rik, Gpr128

MMRRC Submission

040849-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4026 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

56544972-56616218 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 56550661 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Asparagine at position 684 (Y684N)

Ref Sequence

ENSEMBL: ENSMUSP00000023437 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023437]

AlphaFold

Q8BM96

Predicted Effect

probably damaging
Transcript: ENSMUST00000023437
AA Change: Y684N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000023437
Gene: ENSMUSG00000022755
AA Change: Y684N

Domain	Start	End	E-Value	Type
transmembrane domain	12	34	N/A	INTRINSIC
SCOP:d1edmb_	52	76	1e-3	SMART
GPS	376	424	6.16e-8	SMART
Pfam:7tm_2	428	712	4.5e-40	PFAM

Meta Mutation Damage Score

0.7217

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.1%

Validation Efficiency

97% (36/37)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit reduced body weight gain and increased frequency of peristalsis and slow wave potential in the small intestine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahnak	G	T	19: 8,988,663 (GRCm39)	V3316F	probably damaging	Het
Cacna1a	C	T	8: 85,307,962 (GRCm39)	T1409I	probably damaging	Het
Ccdc158	A	G	5: 92,791,666 (GRCm39)	M698T	probably benign	Het
Ces2g	T	A	8: 105,691,377 (GRCm39)	V171E	probably damaging	Het
Ctnna2	A	T	6: 77,613,827 (GRCm39)	D254E	possibly damaging	Het
Dlec1	T	C	9: 118,966,408 (GRCm39)	Y1126H	probably damaging	Het
Dspp	G	A	5: 104,325,563 (GRCm39)	S642N	unknown	Het
Fezf2	A	T	14: 12,343,986 (GRCm38)	C302S	probably damaging	Het
Gm10754	A	G	10: 97,517,978 (GRCm39)		probably benign	Het
Gm5581	G	A	6: 131,144,031 (GRCm39)		noncoding transcript	Het
Gm7929	T	C	14: 41,472,509 (GRCm39)	K21E	probably damaging	Het
Hmcn1	A	T	1: 150,598,120 (GRCm39)	D1727E	probably benign	Het
Lrriq4	T	C	3: 30,704,422 (GRCm39)	V150A	possibly damaging	Het
Micu3	A	G	8: 40,812,496 (GRCm39)		probably benign	Het
Mrpl3	T	C	9: 104,948,685 (GRCm39)		probably null	Het
Myo1e	A	G	9: 70,232,157 (GRCm39)	I229V	probably benign	Het
Ncam1	T	C	9: 49,476,295 (GRCm39)	I265V	probably benign	Het
Ncoa7	T	C	10: 30,598,720 (GRCm39)	T68A	probably benign	Het
Oprk1	T	C	1: 5,668,908 (GRCm39)	V118A	probably benign	Het
Ppp3r1	T	C	11: 17,144,786 (GRCm39)	V133A	probably damaging	Het
Sidt1	A	G	16: 44,102,249 (GRCm39)	S304P	possibly damaging	Het
Srsf6	T	A	2: 162,776,211 (GRCm39)		probably benign	Het
Tas2r105	A	G	6: 131,663,789 (GRCm39)	V213A	probably benign	Het
Tlr12	C	T	4: 128,510,301 (GRCm39)	E650K	probably benign	Het
Top2b	T	G	14: 16,409,189 (GRCm38)	I777M	probably damaging	Het
Trav4-2	T	A	14: 53,656,287 (GRCm39)	D96E	possibly damaging	Het
Vmn1r34	T	A	6: 66,614,688 (GRCm39)	M17L	probably benign	Het
Vmn2r6	A	T	3: 64,445,671 (GRCm39)	S685T	possibly damaging	Het
Vmn2r89	T	C	14: 51,689,500 (GRCm39)	M1T	probably null	Het
Wdr20	A	G	12: 110,759,950 (GRCm39)	T279A	probably benign	Het
Zfp407	A	G	18: 84,577,721 (GRCm39)	S1131P	possibly damaging	Het
Zfyve1	A	G	12: 83,641,296 (GRCm39)	V120A	probably benign	Het

Other mutations in Adgrg7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01783:Adgrg7	APN	16	56,568,282 (GRCm39)	critical splice donor site	probably null
IGL03122:Adgrg7	APN	16	56,590,725 (GRCm39)	splice site	probably benign
orchard	UTSW	16	56,545,342 (GRCm39)	missense	probably damaging	1.00
sevin	UTSW	16	56,562,769 (GRCm39)	missense	probably damaging	1.00
R0632:Adgrg7	UTSW	16	56,562,952 (GRCm39)	missense	possibly damaging	0.89
R0673:Adgrg7	UTSW	16	56,593,849 (GRCm39)	missense	possibly damaging	0.48
R1690:Adgrg7	UTSW	16	56,615,993 (GRCm39)	missense	probably damaging	0.99
R2009:Adgrg7	UTSW	16	56,582,236 (GRCm39)	missense	probably benign	0.08
R2017:Adgrg7	UTSW	16	56,553,169 (GRCm39)	missense	probably benign	0.02
R2132:Adgrg7	UTSW	16	56,588,281 (GRCm39)	missense	probably damaging	1.00
R2153:Adgrg7	UTSW	16	56,572,791 (GRCm39)	missense	possibly damaging	0.75
R2229:Adgrg7	UTSW	16	56,572,766 (GRCm39)	missense	probably benign
R2436:Adgrg7	UTSW	16	56,582,308 (GRCm39)	missense	possibly damaging	0.78
R2878:Adgrg7	UTSW	16	56,570,817 (GRCm39)	missense	probably benign	0.14
R2981:Adgrg7	UTSW	16	56,570,769 (GRCm39)	critical splice donor site	probably null
R4014:Adgrg7	UTSW	16	56,562,651 (GRCm39)	missense	probably damaging	1.00
R4023:Adgrg7	UTSW	16	56,550,661 (GRCm39)	missense	probably damaging	1.00
R4024:Adgrg7	UTSW	16	56,550,661 (GRCm39)	missense	probably damaging	1.00
R4551:Adgrg7	UTSW	16	56,568,375 (GRCm39)	missense	probably damaging	1.00
R4834:Adgrg7	UTSW	16	56,553,232 (GRCm39)	missense	probably damaging	1.00
R5041:Adgrg7	UTSW	16	56,550,711 (GRCm39)	missense	probably benign	0.21
R5145:Adgrg7	UTSW	16	56,562,682 (GRCm39)	missense	probably benign	0.04
R5377:Adgrg7	UTSW	16	56,550,669 (GRCm39)	missense	possibly damaging	0.68
R5549:Adgrg7	UTSW	16	56,570,790 (GRCm39)	missense	probably damaging	1.00
R5915:Adgrg7	UTSW	16	56,550,748 (GRCm39)	splice site	probably null
R5957:Adgrg7	UTSW	16	56,593,790 (GRCm39)	missense	probably damaging	0.96
R6146:Adgrg7	UTSW	16	56,593,829 (GRCm39)	missense	probably benign	0.21
R6198:Adgrg7	UTSW	16	56,597,556 (GRCm39)	missense	possibly damaging	0.64
R6233:Adgrg7	UTSW	16	56,599,005 (GRCm39)	missense	possibly damaging	0.52
R6337:Adgrg7	UTSW	16	56,572,788 (GRCm39)	missense	probably damaging	0.96
R6633:Adgrg7	UTSW	16	56,550,649 (GRCm39)	missense	probably benign	0.05
R6693:Adgrg7	UTSW	16	56,590,587 (GRCm39)	missense	probably damaging	0.97
R6812:Adgrg7	UTSW	16	56,616,161 (GRCm39)	start gained	probably benign
R6841:Adgrg7	UTSW	16	56,570,787 (GRCm39)	missense	probably damaging	1.00
R6868:Adgrg7	UTSW	16	56,593,839 (GRCm39)	missense	probably benign
R7076:Adgrg7	UTSW	16	56,562,769 (GRCm39)	missense	probably damaging	1.00
R7146:Adgrg7	UTSW	16	56,550,605 (GRCm39)	missense	probably damaging	1.00
R7232:Adgrg7	UTSW	16	56,597,515 (GRCm39)	splice site	probably null
R7266:Adgrg7	UTSW	16	56,590,674 (GRCm39)	missense	probably benign	0.00
R7376:Adgrg7	UTSW	16	56,545,342 (GRCm39)	missense	probably damaging	1.00
R7390:Adgrg7	UTSW	16	56,553,207 (GRCm39)	missense	probably damaging	0.98
R7401:Adgrg7	UTSW	16	56,562,781 (GRCm39)	missense	probably benign	0.43
R7496:Adgrg7	UTSW	16	56,553,220 (GRCm39)	missense	probably benign
R7540:Adgrg7	UTSW	16	56,570,792 (GRCm39)	missense	probably damaging	1.00
R8147:Adgrg7	UTSW	16	56,562,876 (GRCm39)	missense	probably damaging	1.00
R8354:Adgrg7	UTSW	16	56,616,045 (GRCm39)	start gained	probably benign
R8372:Adgrg7	UTSW	16	56,616,114 (GRCm39)	start gained	probably benign
R8393:Adgrg7	UTSW	16	56,582,477 (GRCm39)	missense	probably damaging	1.00
R8454:Adgrg7	UTSW	16	56,616,045 (GRCm39)	start gained	probably benign
R8723:Adgrg7	UTSW	16	56,582,282 (GRCm39)	missense	probably benign	0.00
R8891:Adgrg7	UTSW	16	56,572,762 (GRCm39)	missense	probably benign	0.03
R9017:Adgrg7	UTSW	16	56,553,211 (GRCm39)	missense	probably benign	0.01
R9570:Adgrg7	UTSW	16	56,570,813 (GRCm39)	missense	probably damaging	1.00
R9604:Adgrg7	UTSW	16	56,597,570 (GRCm39)	missense	probably damaging	0.99
R9628:Adgrg7	UTSW	16	56,553,193 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AACTAAGCAATGGTCCTCAGG -3'
(R):5'- ACTTTCTTCCGAGGGTGATAGG -3'

Sequencing Primer
(F):5'- CAATGGTCCTCAGGGATTTTACCAG -3'
(R):5'- TCGATGGGCAGTTGAACATGC -3'

Posted On

2015-04-30