Incidental Mutation 'R4027:Nme4'
Institutional Source Beutler Lab
Gene Symbol Nme4
Ensembl Gene ENSMUSG00000024177
Gene NameNME/NM23 nucleoside diphosphate kinase 4
Synonyms2810024O08Rik, 2610027N22Rik, non-metastatic cells 4, protein expressed in, 5730493H09Rik, NM23-M4
MMRRC Submission 040850-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R4027 (G1)
Quality Score225
Status Validated
Chromosomal Location26091734-26095508 bp(-) (GRCm38)
Type of Mutationunclassified (4650 bp from exon)
DNA Base Change (assembly) T to C at 26094222 bp
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000137416 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000025007] [ENSMUST00000040907] [ENSMUST00000053575]
Predicted Effect probably damaging
Transcript: ENSMUST00000025007
AA Change: D46G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000025007
Gene: ENSMUSG00000024177
AA Change: D46G

NDK 36 173 4.09e-83 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000040907
SMART Domains Protein: ENSMUSP00000045621
Gene: ENSMUSG00000036775

Blast:NDK 1 28 5e-9 BLAST
Pfam:adh_short 29 224 3.6e-44 PFAM
Pfam:KR 30 208 3.8e-11 PFAM
Pfam:adh_short_C2 35 271 6.4e-30 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000053575
SMART Domains Protein: ENSMUSP00000137416
Gene: ENSMUSG00000049124

Sm 7 72 3.7e-24 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000134696
Predicted Effect noncoding transcript
Transcript: ENSMUST00000137672
Predicted Effect noncoding transcript
Transcript: ENSMUST00000150280
Predicted Effect noncoding transcript
Transcript: ENSMUST00000150534
Meta Mutation Damage Score 0.9367 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.5%
  • 10x: 97.0%
  • 20x: 94.2%
Validation Efficiency 97% (60/62)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The nucleoside diphosphate (NDP) kinases (EC are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]
Allele List at MGI
Other mutations in this stock
Total: 58 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930402F06Rik A G 2: 35,380,396 F98L probably damaging Het
Adam26b T C 8: 43,520,372 N531S probably benign Het
Ahdc1 T C 4: 133,064,165 S906P possibly damaging Het
Ank A G 15: 27,544,257 N35D probably damaging Het
Ano10 A G 9: 122,252,928 probably benign Het
Atp4a T C 7: 30,724,952 probably null Het
C030005K15Rik T C 10: 97,725,542 Y109C unknown Het
Carmil1 T A 13: 24,067,223 probably benign Het
Ccl2 A G 11: 82,037,059 R110G probably benign Het
Cntnap2 C A 6: 46,856,128 F758L probably benign Het
Cog6 A C 3: 53,002,529 D267E possibly damaging Het
Cyp4f37 G T 17: 32,631,672 E366D probably benign Het
Dcun1d4 A G 5: 73,534,637 D89G probably damaging Het
Dpep1 T C 8: 123,194,153 V24A probably benign Het
Eefsec T C 6: 88,376,250 I48V probably benign Het
Elmo2 G A 2: 165,294,249 Q195* probably null Het
Ephb3 A G 16: 21,221,697 D561G probably damaging Het
Erich2 C A 2: 70,512,790 probably benign Het
Gatm A G 2: 122,597,446 V362A probably damaging Het
Gdf10 A G 14: 33,932,615 M360V probably damaging Het
Gpr141 T C 13: 19,751,825 N260S probably benign Het
Hectd1 T A 12: 51,802,436 probably null Het
Insrr A G 3: 87,809,599 E682G probably benign Het
Itgax A G 7: 128,141,266 I742V possibly damaging Het
Kcnh1 T C 1: 192,276,699 V187A probably benign Het
Kdm6b G T 11: 69,406,268 S419R possibly damaging Het
Kmt2a A T 9: 44,836,693 probably benign Het
Krt10 A G 11: 99,386,193 probably benign Het
Lct G A 1: 128,285,181 R1912C probably benign Het
Lefty1 T C 1: 180,937,781 S305P probably benign Het
Mast3 A G 8: 70,787,908 L279P probably damaging Het
Mfsd4b3 T C 10: 39,947,347 T306A probably benign Het
Mgam G A 6: 40,754,902 R1351Q probably damaging Het
Mknk1 T A 4: 115,864,561 F101I probably damaging Het
Mlc1 T C 15: 88,966,494 I154V probably benign Het
Myo5b T C 18: 74,759,240 I1685T possibly damaging Het
Nacc2 T A 2: 26,060,336 M463L probably benign Het
Nek11 A C 9: 105,244,390 Y443* probably null Het
Nova1 A G 12: 46,817,018 probably benign Het
Olfr651 A T 7: 104,553,323 I135F possibly damaging Het
Parp10 A G 15: 76,241,154 probably null Het
Pkd1l3 T A 8: 109,623,971 S483T possibly damaging Het
Plce1 T C 19: 38,524,265 S3P probably damaging Het
Pnldc1 T C 17: 12,890,779 D400G probably benign Het
Polr2b G A 5: 77,348,405 R1141H possibly damaging Het
Prmt6 A G 3: 110,249,941 I344T probably damaging Het
Psmd2 G A 16: 20,663,205 G896D probably damaging Het
Ranbp17 C T 11: 33,500,718 R73Q possibly damaging Het
Rcn1 G T 2: 105,399,050 Y52* probably null Het
Reck T C 4: 43,922,931 I402T probably damaging Het
Tecpr1 C A 5: 144,206,259 A735S probably benign Het
Tshz1 T C 18: 84,014,829 K485E possibly damaging Het
Ube4a G A 9: 44,949,900 probably benign Het
Vldlr A G 19: 27,238,313 T237A probably benign Het
Vmn1r74 C A 7: 11,846,971 T66K probably damaging Het
Wdr49 C A 3: 75,323,665 L563F probably benign Het
Zmym1 C T 4: 127,049,879 V239I probably benign Het
Zmym5 T A 14: 56,797,811 T267S probably benign Het
Other mutations in Nme4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01455:Nme4 APN 17 26092062 missense probably benign 0.00
IGL01725:Nme4 APN 17 26092066 missense probably benign 0.05
IGL02217:Nme4 APN 17 26093860 missense probably damaging 0.99
R0153:Nme4 UTSW 17 26093857 critical splice donor site probably null
R1839:Nme4 UTSW 17 26092097 missense probably damaging 1.00
R2205:Nme4 UTSW 17 26092140 missense possibly damaging 0.95
R4029:Nme4 UTSW 17 26094222 unclassified probably null
R5023:Nme4 UTSW 17 26093668 missense probably benign 0.11
R5044:Nme4 UTSW 17 26093833 unclassified probably benign
R5635:Nme4 UTSW 17 26094231 missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On2015-04-30