Incidental Mutation 'R4032:Zfp87'
| ID |
313550 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp87
|
| Ensembl Gene |
ENSMUSG00000097333 |
| Gene Name |
zinc finger protein 87 |
| Synonyms |
Mzf22, 2210039O17Rik, 4631412O18Rik, Zfp72, KRAB4 |
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.096)
|
| Stock # |
R4032 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
13 |
| Chromosomal Location |
67663900-67674296 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 74520449 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Alanine
at position 210
(T210A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000152502
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000091481]
[ENSMUST00000223163]
|
| AlphaFold |
Q8K2A4 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000091481
AA Change: T210A
PolyPhen 2
Score 0.617 (Sensitivity: 0.87; Specificity: 0.91)
|
| SMART Domains |
Protein: ENSMUSP00000089063
Gene: ENSMUSG00000069184
AA Change: T210A
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
1 |
32 |
3.57e-1 |
SMART |
|
ZnF_C2H2
|
48 |
70 |
5.42e-2 |
SMART |
|
ZnF_C2H2
|
76 |
98 |
1.18e-2 |
SMART |
|
ZnF_C2H2
|
104 |
126 |
2.05e-2 |
SMART |
|
ZnF_C2H2
|
132 |
154 |
2.3e-5 |
SMART |
|
ZnF_C2H2
|
160 |
181 |
2.45e0 |
SMART |
|
ZnF_C2H2
|
187 |
209 |
1.92e-2 |
SMART |
|
ZnF_C2H2
|
215 |
237 |
5.59e-4 |
SMART |
|
ZnF_C2H2
|
243 |
265 |
4.87e-4 |
SMART |
|
ZnF_C2H2
|
271 |
293 |
4.94e-5 |
SMART |
|
ZnF_C2H2
|
299 |
321 |
9.08e-4 |
SMART |
|
ZnF_C2H2
|
327 |
349 |
1.13e-4 |
SMART |
|
ZnF_C2H2
|
355 |
377 |
3.16e-3 |
SMART |
|
ZnF_C2H2
|
383 |
405 |
2.24e-3 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000223163
AA Change: T210A
PolyPhen 2
Score 0.617 (Sensitivity: 0.87; Specificity: 0.91)
|
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.4%
- 10x: 96.8%
- 20x: 93.3%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 36 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Alkbh2 |
C |
T |
5: 114,262,287 (GRCm39) |
E148K |
probably damaging |
Het |
| Arhgap17 |
G |
T |
7: 122,879,289 (GRCm39) |
|
probably benign |
Het |
| Atp13a4 |
T |
A |
16: 29,237,389 (GRCm39) |
M846L |
probably damaging |
Het |
| Capn5 |
G |
T |
7: 97,778,453 (GRCm39) |
Q389K |
probably damaging |
Het |
| Cdkl3 |
T |
A |
11: 51,902,118 (GRCm39) |
I109N |
probably damaging |
Het |
| Cry2 |
T |
C |
2: 92,244,172 (GRCm39) |
I334V |
probably benign |
Het |
| Cs |
T |
C |
10: 128,196,913 (GRCm39) |
L436P |
probably damaging |
Het |
| D17H6S53E |
T |
C |
17: 35,346,355 (GRCm39) |
S89P |
probably benign |
Het |
| Dync1h1 |
C |
T |
12: 110,584,483 (GRCm39) |
Q629* |
probably null |
Het |
| H4c12 |
T |
C |
13: 21,934,588 (GRCm39) |
I30V |
possibly damaging |
Het |
| Itpka |
C |
T |
2: 119,573,082 (GRCm39) |
P75S |
probably benign |
Het |
| Lyst |
C |
T |
13: 13,791,250 (GRCm39) |
H38Y |
probably damaging |
Het |
| Mansc4 |
T |
C |
6: 146,976,678 (GRCm39) |
K313E |
probably benign |
Het |
| Megf6 |
T |
A |
4: 154,261,550 (GRCm39) |
C58* |
probably null |
Het |
| Mtor |
T |
C |
4: 148,621,209 (GRCm39) |
V1869A |
probably benign |
Het |
| Mybpc1 |
T |
C |
10: 88,365,426 (GRCm39) |
D899G |
probably benign |
Het |
| Nek10 |
C |
A |
14: 14,853,877 (GRCm38) |
|
probably null |
Het |
| Nfkb1 |
T |
C |
3: 135,300,110 (GRCm39) |
S657G |
possibly damaging |
Het |
| Or10d5j |
T |
C |
9: 39,867,629 (GRCm39) |
T213A |
probably benign |
Het |
| Or2a25 |
A |
T |
6: 42,888,559 (GRCm39) |
Y34F |
probably benign |
Het |
| Or4m1 |
T |
A |
14: 50,557,767 (GRCm39) |
D175V |
possibly damaging |
Het |
| Or4n5 |
G |
T |
14: 50,132,433 (GRCm39) |
H275Q |
probably benign |
Het |
| Or51f23 |
A |
G |
7: 102,453,396 (GRCm39) |
K237R |
probably benign |
Het |
| Pabir1 |
C |
T |
19: 24,454,106 (GRCm39) |
M205I |
probably benign |
Het |
| Pde1b |
A |
G |
15: 103,429,753 (GRCm39) |
D82G |
probably damaging |
Het |
| Pdzrn4 |
C |
T |
15: 92,667,414 (GRCm39) |
T522M |
probably damaging |
Het |
| Ptpro |
C |
A |
6: 137,438,740 (GRCm39) |
N373K |
probably damaging |
Het |
| Ptprs |
T |
C |
17: 56,720,386 (GRCm39) |
Y1478C |
probably damaging |
Het |
| Sdc2 |
A |
T |
15: 33,017,323 (GRCm39) |
I46F |
probably damaging |
Het |
| Sergef |
C |
A |
7: 46,092,726 (GRCm39) |
E428* |
probably null |
Het |
| Stk36 |
A |
G |
1: 74,665,207 (GRCm39) |
S651G |
probably benign |
Het |
| Ubr5 |
A |
G |
15: 38,025,081 (GRCm39) |
V559A |
|
Het |
| Ugt8a |
C |
A |
3: 125,667,807 (GRCm39) |
D345Y |
probably damaging |
Het |
| Usp44 |
A |
G |
10: 93,683,127 (GRCm39) |
|
probably benign |
Het |
| Vps13a |
A |
T |
19: 16,594,263 (GRCm39) |
L3156Q |
probably damaging |
Het |
| Zfp655 |
A |
G |
5: 145,180,858 (GRCm39) |
T239A |
possibly damaging |
Het |
|
| Other mutations in Zfp87 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02165:Zfp87
|
APN |
13 |
67,665,989 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02372:Zfp87
|
APN |
13 |
67,668,739 (GRCm39) |
splice site |
probably benign |
|
|
IGL03160:Zfp87
|
APN |
13 |
67,669,392 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0653:Zfp87
|
UTSW |
13 |
74,520,190 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0711:Zfp87
|
UTSW |
13 |
74,524,544 (GRCm39) |
splice site |
probably benign |
|
|
R1498:Zfp87
|
UTSW |
13 |
74,520,736 (GRCm39) |
missense |
probably benign |
0.01 |
|
R3801:Zfp87
|
UTSW |
13 |
67,669,334 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4629:Zfp87
|
UTSW |
13 |
74,520,512 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4950:Zfp87
|
UTSW |
13 |
67,666,018 (GRCm39) |
missense |
probably benign |
0.20 |
|
R5604:Zfp87
|
UTSW |
13 |
67,665,945 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6111:Zfp87
|
UTSW |
13 |
74,520,504 (GRCm39) |
missense |
probably benign |
0.31 |
|
R6130:Zfp87
|
UTSW |
13 |
74,520,460 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R6277:Zfp87
|
UTSW |
13 |
74,520,643 (GRCm39) |
nonsense |
probably null |
|
|
R6392:Zfp87
|
UTSW |
13 |
67,664,986 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6800:Zfp87
|
UTSW |
13 |
74,520,080 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6909:Zfp87
|
UTSW |
13 |
74,519,861 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R7009:Zfp87
|
UTSW |
13 |
67,665,173 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7183:Zfp87
|
UTSW |
13 |
67,665,593 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7298:Zfp87
|
UTSW |
13 |
74,520,513 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R7330:Zfp87
|
UTSW |
13 |
74,523,153 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7341:Zfp87
|
UTSW |
13 |
74,520,467 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R7448:Zfp87
|
UTSW |
13 |
67,665,163 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7597:Zfp87
|
UTSW |
13 |
67,665,412 (GRCm39) |
missense |
probably benign |
0.06 |
|
R8696:Zfp87
|
UTSW |
13 |
74,520,599 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9280:Zfp87
|
UTSW |
13 |
74,520,803 (GRCm39) |
missense |
probably benign |
0.16 |
|
R9429:Zfp87
|
UTSW |
13 |
74,520,703 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9780:Zfp87
|
UTSW |
13 |
67,665,241 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9782:Zfp87
|
UTSW |
13 |
74,520,932 (GRCm39) |
missense |
probably benign |
0.00 |
|
RF014:Zfp87
|
UTSW |
13 |
74,523,173 (GRCm39) |
missense |
probably benign |
0.17 |
|
Z1176:Zfp87
|
UTSW |
13 |
67,674,275 (GRCm39) |
start gained |
probably benign |
|
|
Z1177:Zfp87
|
UTSW |
13 |
74,519,911 (GRCm39) |
missense |
probably damaging |
0.99 |
|
| Predicted Primers |
PCR Primer
(F):5'- AAGGCTTTGCTACATTCTTCACA -3'
(R):5'- ATGTGGCAAAGCTTTTAACTGGTC -3'
Sequencing Primer
(F):5'- CCACATTCGCTGCATTTGTAGGG -3'
(R):5'- GGCAAAGCTTTTAACTGGTCTTCAAG -3'
|
| Posted On |
2015-04-30 |
Incidental Mutation