Mutagenetix > Incidental Mutation

Incidental Mutation 'R4036:Mid1-ps1'

313734

Institutional Source

Beutler Lab

Gene Symbol

Mid1-ps1

Ensembl Gene

Gene Name

midline 1, pseudogene 1

Synonyms

Gm21857

MMRRC Submission

040963-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.671) question?

Stock #

R4036 (G1)

Quality Score

128

Status

Validated

Chromosome

Chromosomal Location

90764326-90774754 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

G to A at 90773563 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000167967

SMART Domains

Protein: ENSMUSP00000126476
Gene: ENSMUSG00000095134

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Blast:BBC	21	78	2e-29	BLAST
FN3	115	205	2.19e-7	SMART
Pfam:PRY	219	268	1.7e-12	PFAM
SPRY	271	390	1.12e-31	SMART

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.8%
20x: 93.4%

Validation Efficiency

100% (33/33)

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930544M13Rik	T	C	13: 114,744,205 (GRCm39)		noncoding transcript	Het
Ahctf1	T	C	1: 179,590,181 (GRCm39)	Q1364R	possibly damaging	Het
Anxa2	A	G	9: 69,395,352 (GRCm39)	N265S	probably damaging	Het
Bcan	A	G	3: 87,903,423 (GRCm39)		probably null	Het
Cntln	A	G	4: 84,924,725 (GRCm39)	Y610C	probably damaging	Het
Cyp39a1	C	A	17: 43,987,831 (GRCm39)	A99E	probably damaging	Het
Drc1	A	G	5: 30,504,526 (GRCm39)	I223V	probably benign	Het
Eef2kmt	A	T	16: 5,063,135 (GRCm39)	V335D	probably damaging	Het
Fanci	T	C	7: 79,094,570 (GRCm39)	L1167P	probably damaging	Het
Glb1l3	A	C	9: 26,740,343 (GRCm39)	M329R	probably damaging	Het
Gns	C	A	10: 121,207,095 (GRCm39)	T72N	probably damaging	Het
Hspa2	T	C	12: 76,452,542 (GRCm39)	V412A	probably damaging	Het
Klk1b8	A	G	7: 43,447,511 (GRCm39)	I9V	probably null	Het
Map4	A	G	9: 109,861,283 (GRCm39)	T216A	possibly damaging	Het
Metrn	T	C	17: 26,013,984 (GRCm39)	T281A	probably benign	Het
Ndc1	G	A	4: 107,268,269 (GRCm39)	D602N	probably benign	Het
Nsd1	A	G	13: 55,361,524 (GRCm39)	D164G	possibly damaging	Het
Or5ac22	T	A	16: 59,135,113 (GRCm39)	H219L	probably benign	Het
Pcdha8	T	C	18: 37,125,914 (GRCm39)	M132T	probably benign	Het
Pomt2	A	G	12: 87,158,296 (GRCm39)		probably null	Het
Prrt3	AGGGGG	AGGGG	6: 113,474,641 (GRCm39)		probably null	Het
Rfx6	T	A	10: 51,602,842 (GRCm39)	H786Q	probably damaging	Het
Scn4a	A	C	11: 106,212,883 (GRCm39)	L1374R	possibly damaging	Het
Slc16a9	C	T	10: 70,110,786 (GRCm39)	T124M	probably damaging	Het
Slc46a2	A	T	4: 59,913,818 (GRCm39)	F368L	probably damaging	Het
St18	T	A	1: 6,898,010 (GRCm39)	I604K	probably damaging	Het
Ttn	A	G	2: 76,548,497 (GRCm39)	V31935A	possibly damaging	Het
Urb1	T	C	16: 90,584,974 (GRCm39)	H549R	probably benign	Het
Vmn1r219	A	G	13: 23,347,272 (GRCm39)	M154V	probably benign	Het
Zfp575	G	A	7: 24,285,440 (GRCm39)	S67L	possibly damaging	Het

Other mutations in Mid1-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R4038:Mid1-ps1	UTSW	Y	90,773,563 (GRCm39)	splice site	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- GTCCCACGACAACCTGACTG -3'
(R):5'- CCAGAGAATCTCGATTTTCCATTCC -3'

Sequencing Primer
(F):5'- ACAACCTGACTGTCGAGCG -3'
(R):5'- GAGCAGACAGACTGATGGCC -3'

Posted On

2015-04-30