Mutagenetix > Incidental Mutation

Incidental Mutation 'R4043:Olfr855'

313938

Institutional Source

Beutler Lab

Gene Symbol

Olfr855

Ensembl Gene

ENSMUSG00000043087

Gene Name

olfactory receptor 855

Synonyms

MOR148-1, GA_x6K02T2PVTD-13330461-13331399

MMRRC Submission

041615-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.117) question?

Stock #

R4043 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19582617-19586030 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 19584995 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Phenylalanine at position 153 (V153F)

Ref Sequence

ENSEMBL: ENSMUSP00000150218 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061693] [ENSMUST00000215587]

AlphaFold

Q7TRF8

Predicted Effect

probably benign
Transcript: ENSMUST00000061693
AA Change: V153F

PolyPhen 2 Score 0.162 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000054790
Gene: ENSMUSG00000043087
AA Change: V153F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	310	1.7e-54	PFAM
Pfam:7TM_GPCR_Srsx	37	182	5.2e-8	PFAM
Pfam:7tm_1	43	292	2.5e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215587
AA Change: V153F

PolyPhen 2 Score 0.162 (Sensitivity: 0.92; Specificity: 0.87)

Meta Mutation Damage Score

0.0816

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 92.9%

Validation Efficiency

97% (64/66)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4430402I18Rik	A	T	19: 28,945,783	C80S	possibly damaging	Het
4930415L06Rik	A	G	X: 89,932,303	F96S	probably damaging	Het
Adgrf3	T	C	5: 30,204,362	N44D	probably benign	Het
AU018091	A	G	7: 3,159,122	F375L	probably damaging	Het
Bsph1	G	T	7: 13,458,276		probably null	Het
Casp1	A	G	9: 5,302,444	D122G	probably benign	Het
Ccdc146	C	T	5: 21,316,943	C361Y	probably benign	Het
Cdc37l1	T	C	19: 28,990,628	S31P	possibly damaging	Het
Cdca2	A	T	14: 67,704,006	M249K	probably benign	Het
Cfap73	C	T	5: 120,629,965		probably null	Het
Cgnl1	A	G	9: 71,705,293	L749S	probably damaging	Het
Cmtr2	C	A	8: 110,221,830	C257*	probably null	Het
Col6a4	A	T	9: 106,072,411	L675*	probably null	Het
Cpne8	A	T	15: 90,572,001	D186E	probably damaging	Het
Csmd3	A	G	15: 47,755,966		probably null	Het
D330045A20Rik	C	T	X: 139,507,003	S364L	probably damaging	Het
D430042O09Rik	A	G	7: 125,868,741	I1366V	probably benign	Het
Daglb	T	A	5: 143,487,151	Y354N	possibly damaging	Het
Dlgap1	T	C	17: 70,761,080	S549P	probably damaging	Het
Dnah11	T	C	12: 117,879,943	D4389G	probably damaging	Het
Dst	T	A	1: 34,190,684	C2631S	probably benign	Het
Gimap1	T	A	6: 48,743,242	W263R	probably damaging	Het
Gne	A	C	4: 44,040,383	C594G	possibly damaging	Het
Gtf2a1	T	C	12: 91,575,667	H47R	probably benign	Het
Hdac6	T	C	X: 7,931,492	T993A	probably benign	Het
Helz2	T	C	2: 181,229,710	D2703G	probably benign	Het
Jmjd1c	G	A	10: 67,219,466	V222I	possibly damaging	Het
Kndc1	A	G	7: 139,924,129	E1116G	probably benign	Het
Krt10	C	T	11: 99,386,993		probably null	Het
Lrrc37a	T	G	11: 103,498,653	H1982P	possibly damaging	Het
Med13l	T	A	5: 118,593,463	L68Q	probably damaging	Het
Megf10	C	A	18: 57,259,798	D422E	probably damaging	Het
Mpz	C	T	1: 171,159,771		probably benign	Het
Muc5ac	A	T	7: 141,807,478	T1508S	possibly damaging	Het
Myo3a	C	T	2: 22,333,539		probably benign	Het
Ndnf	A	T	6: 65,703,936	N400Y	possibly damaging	Het
Olfr192	G	A	16: 59,098,761	T77I	unknown	Het
Olfr414	T	C	1: 174,431,091	I221T	probably damaging	Het
Otol1	A	G	3: 70,027,779	D368G	probably damaging	Het
Pappa	A	G	4: 65,314,587	N1321S	probably benign	Het
Patl1	T	A	19: 11,942,950	L756Q	probably damaging	Het
Plcg2	A	G	8: 117,612,978	M1043V	probably benign	Het
Plekhg2	G	A	7: 28,364,719		probably benign	Het
Ppp2r2d	G	A	7: 138,882,416	W265*	probably null	Het
Prss40	G	T	1: 34,560,879	S9*	probably null	Het
Radil	T	A	5: 142,494,233	I471F	probably benign	Het
Rpl10l	A	T	12: 66,284,203	M52K	probably damaging	Het
Scn1a	A	T	2: 66,326,036	S510T	possibly damaging	Het
Sdad1	T	G	5: 92,302,694	N194T	probably damaging	Het
Sel1l3	A	T	5: 53,188,054	Y259*	probably null	Het
Slc22a26	T	C	19: 7,788,329		probably null	Het
Snap91	C	T	9: 86,777,049	G477D	probably damaging	Het
Srr	T	C	11: 74,909,121	T202A	probably benign	Het
Srrm1	C	T	4: 135,340,931		probably benign	Het
Trp53bp2	T	A	1: 182,449,061	L869Q	possibly damaging	Het
Ttbk1	T	A	17: 46,446,762	D982V	probably benign	Het
Ttn	T	C	2: 76,794,157	T15324A	probably benign	Het
Ush1c	T	C	7: 46,221,528	E276G	probably damaging	Het
Vcan	T	A	13: 89,692,543	L1627F	probably benign	Het
Vps8	A	C	16: 21,526,396	D823A	probably damaging	Het
Zfp616	T	A	11: 74,085,282	N792K	possibly damaging	Het
Zfpm2	A	T	15: 40,870,627	D2V	possibly damaging	Het
Zfyve16	A	C	13: 92,513,763		probably null	Het
Zmym2	T	A	14: 56,958,308		probably benign	Het

Other mutations in Olfr855

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01020:Olfr855	APN	9	19585320	missense	possibly damaging	0.74
IGL01405:Olfr855	APN	9	19585205	missense	probably benign	0.23
IGL01775:Olfr855	APN	9	19584705	missense	probably benign	0.04
IGL01920:Olfr855	APN	9	19585022	missense	probably benign	0.01
R0501:Olfr855	UTSW	9	19584618	missense	probably damaging	1.00
R0600:Olfr855	UTSW	9	19585304	missense	possibly damaging	0.47
R0667:Olfr855	UTSW	9	19585447	missense	probably benign
R1769:Olfr855	UTSW	9	19585386	missense	probably damaging	0.98
R3117:Olfr855	UTSW	9	19584941	missense	probably damaging	0.99
R4002:Olfr855	UTSW	9	19584714	missense	probably damaging	1.00
R4003:Olfr855	UTSW	9	19584714	missense	probably damaging	1.00
R4243:Olfr855	UTSW	9	19584558	missense	probably damaging	1.00
R4672:Olfr855	UTSW	9	19585430	missense	possibly damaging	0.74
R4673:Olfr855	UTSW	9	19585430	missense	possibly damaging	0.74
R4959:Olfr855	UTSW	9	19585208	missense	probably benign
R4973:Olfr855	UTSW	9	19585208	missense	probably benign
R5223:Olfr855	UTSW	9	19585026	missense	probably benign	0.16
R5681:Olfr855	UTSW	9	19584899	missense	probably damaging	1.00
R6005:Olfr855	UTSW	9	19584885	missense	probably benign	0.45
R6017:Olfr855	UTSW	9	19585434	missense	probably benign	0.00
R6145:Olfr855	UTSW	9	19584888	missense	probably benign	0.02
R6615:Olfr855	UTSW	9	19584989	missense	probably benign	0.05
R6771:Olfr855	UTSW	9	19585379	missense	probably benign	0.16
R6969:Olfr855	UTSW	9	19584590	missense	possibly damaging	0.77
R7239:Olfr855	UTSW	9	19585191	missense	probably damaging	1.00
R7313:Olfr855	UTSW	9	19584642	missense	probably damaging	1.00
R7361:Olfr855	UTSW	9	19584560	missense	probably benign	0.00
R8112:Olfr855	UTSW	9	19584724	missense	probably benign	0.44
R8470:Olfr855	UTSW	9	19584969	missense	probably damaging	0.99
R9155:Olfr855	UTSW	9	19585083	missense	probably benign	0.00
R9187:Olfr855	UTSW	9	19584654	missense	probably benign	0.03
R9422:Olfr855	UTSW	9	19584672	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGCATCACTTACACAGACTGC -3'
(R):5'- ACAAGTGAGTCCCGCAAGTAG -3'

Sequencing Primer
(F):5'- AGACTGCCTCACACAGCTCTTC -3'
(R):5'- TCCCGCAAGTAGAGAAGGCTTTATAC -3'

Posted On

2015-04-30