Mutagenetix > Incidental Mutation

Incidental Mutation 'R4049:Oprm1'

314047

Institutional Source

Beutler Lab

Gene Symbol

Oprm1

Ensembl Gene

ENSMUSG00000000766

Gene Name

opioid receptor, mu 1

Synonyms

MOP-R, MOR-1, MOP receptor, mor, Oprm, muOR

MMRRC Submission

041616-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.110) question?

Stock #

R4049 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

6708593-6988209 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 6779087 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 95 (V95I)

Ref Sequence

ENSEMBL: ENSMUSP00000101240 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000783] [ENSMUST00000052751] [ENSMUST00000056385] [ENSMUST00000063036] [ENSMUST00000078634] [ENSMUST00000092729] [ENSMUST00000092731] [ENSMUST00000105604] [ENSMUST00000092734] [ENSMUST00000105602] [ENSMUST00000105603] [ENSMUST00000105605] [ENSMUST00000105607] [ENSMUST00000105611] [ENSMUST00000129221] [ENSMUST00000129954] [ENSMUST00000105615] [ENSMUST00000123861] [ENSMUST00000105597] [ENSMUST00000105601] [ENSMUST00000135502] [ENSMUST00000144264] [ENSMUST00000154906] [ENSMUST00000154941] [ENSMUST00000147171] [ENSMUST00000152674] [ENSMUST00000150374] [ENSMUST00000143875]

AlphaFold

P42866

Predicted Effect

probably benign
Transcript: ENSMUST00000000783
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000000783
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	339	1.6e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.3e-10	PFAM
Pfam:7tm_1	85	336	4e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000052751
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000060329
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.2e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000056385
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000060590
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.3e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000063036
AA Change: V95I

PolyPhen 2 Score 0.044 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000053498
Gene: ENSMUSG00000000766
AA Change: V95I

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	268	8.7e-64	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000078634
AA Change: V163I

PolyPhen 2 Score 0.108 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000077704
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	339	2.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	2.4e-10	PFAM
Pfam:7tm_1	85	336	9e-60	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000092729
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000090405
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.6e-11	PFAM
Pfam:7tm_1	85	336	3.1e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000092731
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000090407
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.9e-11	PFAM
Pfam:7tm_1	85	336	3.2e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105604
AA Change: V163I

PolyPhen 2 Score 0.089 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000101229
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	332	5.1e-8	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.9e-11	PFAM
Pfam:7tm_1	85	336	3.8e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000092734
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000090410
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.3e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105602
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000101227
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.8e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.8e-11	PFAM
Pfam:7tm_1	85	336	3.1e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105603
AA Change: V163I

PolyPhen 2 Score 0.105 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000101228
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	6.7e-8	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.6e-11	PFAM
Pfam:7tm_1	85	336	3.6e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105605
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000101230
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	9.8e-11	PFAM
Pfam:7tm_1	85	336	3.2e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105607
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000101232
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.3e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105611
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000101236
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	338	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.4e-10	PFAM
Pfam:7tm_1	85	336	4.4e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000129221
AA Change: V99I

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000123117
Gene: ENSMUSG00000000766
AA Change: V99I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	12	261	1.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	15	287	7.3e-11	PFAM
Pfam:7tm_1	21	272	2.4e-67	PFAM
low complexity region	340	351	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000129954
AA Change: V163I

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000122385
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	338	6.9e-8	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.5e-10	PFAM
Pfam:7tm_1	85	336	5.4e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105615
AA Change: V95I

PolyPhen 2 Score 0.358 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000101240
Gene: ENSMUSG00000000766
AA Change: V95I

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	268	1.3e-63	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133486

Predicted Effect

probably benign
Transcript: ENSMUST00000123861

SMART Domains

Protein: ENSMUSP00000120187
Gene: ENSMUSG00000000766

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	46	100	3e-9	SMART
PDB:4DKL\|A	52	100	3e-21	PDB
low complexity region	119	131	N/A	INTRINSIC
low complexity region	173	184	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000105597

Predicted Effect

probably benign
Transcript: ENSMUST00000105601

SMART Domains

Protein: ENSMUSP00000101226
Gene: ENSMUSG00000000766

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	46	100	3e-9	SMART
PDB:4DKL\|A	52	100	3e-21	PDB
low complexity region	119	131	N/A	INTRINSIC
low complexity region	173	184	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135502
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000135143
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	339	2.2e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.9e-10	PFAM
Pfam:7tm_1	85	336	7.5e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000144264
AA Change: V163I

PolyPhen 2 Score 0.303 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000115836
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	325	1.7e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1e-10	PFAM
Pfam:7tm_1	85	336	3.4e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000154906
AA Change: V163I

PolyPhen 2 Score 0.016 (Sensitivity: 0.95; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000114342
Gene: ENSMUSG00000000766
AA Change: V163I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	76	332	1.5e-7	PFAM
Pfam:7TM_GPCR_Srsx	79	351	1.1e-10	PFAM
Pfam:7tm_1	85	336	3.6e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000154941
AA Change: V99I

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000115413
Gene: ENSMUSG00000000766
AA Change: V99I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	12	261	9.6e-8	PFAM
Pfam:7TM_GPCR_Srsx	15	287	6.1e-11	PFAM
Pfam:7tm_1	21	272	2e-67	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000147171
AA Change: V95I

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000117950
Gene: ENSMUSG00000000766
AA Change: V95I

Domain	Start	End	E-Value	Type
Pfam:7tm_1	24	268	9.2e-64	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148625

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141897

Predicted Effect

probably benign
Transcript: ENSMUST00000152674

SMART Domains

Protein: ENSMUSP00000115552
Gene: ENSMUSG00000000766

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	46	94	8e-8	SMART
PDB:4DKL\|A	52	94	7e-23	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000150374

Predicted Effect

probably benign
Transcript: ENSMUST00000143875

Meta Mutation Damage Score

0.0985

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.6%

Validation Efficiency

100% (65/65)

MGI Phenotype

FUNCTION: This gene encodes the mu opioid receptor which is where drugs such as morphine and other opioids have pharmacological effects. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
PHENOTYPE: Homozygotes for null mutations exhibit isoform dependent loss of behavioral and gastrointestinal opioid responses and may also show impaired spatial memory, heightened nociception, reduced locomotor activity, increased hematopoietic proliferation, and decreased male fertility. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb5	T	A	12: 118,832,404 (GRCm39)	E1190V	probably damaging	Het
Arhgef10	A	G	8: 15,029,998 (GRCm39)	T928A	probably benign	Het
Arhgef10l	T	A	4: 140,242,762 (GRCm39)	I836F	probably benign	Het
Blm	T	A	7: 80,152,610 (GRCm39)	T446S	probably benign	Het
Ccdc63	T	A	5: 122,260,813 (GRCm39)	Q237L	probably damaging	Het
Ccn5	A	G	2: 163,670,904 (GRCm39)	D137G	probably damaging	Het
Cep57l1	G	T	10: 41,605,356 (GRCm39)	R130S	probably damaging	Het
Clstn2	T	G	9: 97,339,613 (GRCm39)	E786A	possibly damaging	Het
Col16a1	C	T	4: 129,962,545 (GRCm39)	P540L	probably damaging	Het
Csf2	A	G	11: 54,140,159 (GRCm39)	F61L	probably damaging	Het
Ctr9	C	T	7: 110,654,750 (GRCm39)	R1094C	unknown	Het
Cyp3a41a	A	G	5: 145,650,350 (GRCm39)	C98R	probably damaging	Het
Dthd1	T	A	5: 62,984,508 (GRCm39)	C404*	probably null	Het
Egfem1	A	C	3: 29,740,880 (GRCm39)	H518P	probably benign	Het
Elf3	T	C	1: 135,182,015 (GRCm39)	S369G	probably benign	Het
Eml6	C	T	11: 29,788,577 (GRCm39)	V503M	probably damaging	Het
Enthd1	T	C	15: 80,444,240 (GRCm39)	D105G	probably damaging	Het
Eral1	A	G	11: 77,966,428 (GRCm39)	L250P	probably damaging	Het
Gdf15	A	T	8: 71,082,605 (GRCm39)	M167K	probably benign	Het
Ggps1	T	C	13: 14,228,284 (GRCm39)	K300E	probably benign	Het
Gm8074	G	A	9: 78,229,618 (GRCm39)		noncoding transcript	Het
Herc3	A	G	6: 58,853,822 (GRCm39)	I623V	probably damaging	Het
Mad1l1	A	G	5: 140,118,571 (GRCm39)	S457P	probably damaging	Het
Map1lc3a	G	T	2: 155,119,462 (GRCm39)	V91F	possibly damaging	Het
Map4k3	A	G	17: 80,913,394 (GRCm39)	V617A	probably benign	Het
Mov10l1	T	A	15: 88,879,235 (GRCm39)		probably benign	Het
Mroh2a	A	G	1: 88,186,386 (GRCm39)	S64G	probably benign	Het
Ncor1	G	T	11: 62,220,494 (GRCm39)		probably null	Het
Nfe2	C	T	15: 103,159,364 (GRCm39)	E36K	possibly damaging	Het
Or4a78	T	C	2: 89,498,006 (GRCm39)	I75V	probably benign	Het
Or4c107	A	G	2: 88,789,617 (GRCm39)	K269R	probably benign	Het
Or52d3	T	C	7: 104,229,575 (GRCm39)	F241L	probably benign	Het
Or5d46	T	C	2: 88,174,144 (GRCm39)		probably null	Het
Pcdha9	T	A	18: 37,130,995 (GRCm39)	H21Q	probably benign	Het
Pcolce2	A	T	9: 95,520,808 (GRCm39)	I62F	probably damaging	Het
Pfpl	T	A	19: 12,407,053 (GRCm39)	C435S	probably damaging	Het
Pkhd1l1	G	A	15: 44,361,953 (GRCm39)	C542Y	probably damaging	Het
Plekha5	T	C	6: 140,529,597 (GRCm39)	S75P	probably damaging	Het
Pphln1	C	A	15: 93,362,987 (GRCm39)	A202E	probably damaging	Het
Ppp1r1a	A	G	15: 103,440,881 (GRCm39)	L92P	probably damaging	Het
Prokr2	T	C	2: 132,223,414 (GRCm39)	T43A	probably benign	Het
Rai14	T	C	15: 10,592,298 (GRCm39)	N199S	probably benign	Het
Rasgrp2	T	C	19: 6,454,757 (GRCm39)	L199P	probably damaging	Het
Rnf213	A	C	11: 119,373,274 (GRCm39)	M4939L	possibly damaging	Het
Slc23a2	C	T	2: 131,902,603 (GRCm39)	R533Q	probably benign	Het
Slc7a7	T	C	14: 54,610,548 (GRCm39)		probably null	Het
Snrpd2	T	A	7: 18,885,232 (GRCm39)	V31E	probably damaging	Het
Spire1	G	T	18: 67,662,101 (GRCm39)		probably null	Het
Srsf4	T	C	4: 131,627,854 (GRCm39)		probably benign	Het
Tcf20	A	G	15: 82,737,630 (GRCm39)	S1274P	probably damaging	Het
Tcof1	C	A	18: 60,965,975 (GRCm39)	A376S	possibly damaging	Het
Thsd1	T	A	8: 22,733,180 (GRCm39)	Y76N	possibly damaging	Het
Timm44	A	G	8: 4,310,561 (GRCm39)	V397A	probably benign	Het
Tmc6	G	A	11: 117,669,087 (GRCm39)	T89I	possibly damaging	Het
Trbv31	C	A	6: 41,534,639 (GRCm39)	C107F	probably damaging	Het
Trim37	T	A	11: 87,031,429 (GRCm39)		probably null	Het
Ttll5	T	C	12: 86,059,573 (GRCm39)	V1226A	probably benign	Het
Ube3b	T	C	5: 114,550,931 (GRCm39)	V865A	probably benign	Het
Vmn2r86	A	T	10: 130,282,966 (GRCm39)	M550K	probably damaging	Het
Vps54	C	A	11: 21,250,183 (GRCm39)	T373N	probably benign	Het
Wdr64	T	G	1: 175,633,422 (GRCm39)	I891S	probably benign	Het
Zfhx4	C	T	3: 5,463,919 (GRCm39)	S1384L	probably damaging	Het
Zfp703	A	G	8: 27,469,113 (GRCm39)	E259G	possibly damaging	Het
Zfp933	T	C	4: 147,910,969 (GRCm39)	H209R	probably damaging	Het
Zfp980	A	G	4: 145,429,170 (GRCm39)	H633R	probably damaging	Het

Other mutations in Oprm1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01018:Oprm1	APN	10	6,987,170 (GRCm39)	utr 3 prime	probably benign
IGL01768:Oprm1	APN	10	6,779,186 (GRCm39)	missense	probably damaging	1.00
IGL02455:Oprm1	APN	10	6,780,219 (GRCm39)	missense	probably damaging	1.00
IGL03391:Oprm1	APN	10	6,964,077 (GRCm39)	intron	probably benign
IGL03410:Oprm1	APN	10	6,780,051 (GRCm39)	missense	probably damaging	1.00
IGL03048:Oprm1	UTSW	10	6,779,064 (GRCm39)	missense	probably damaging	1.00
R0189:Oprm1	UTSW	10	6,739,071 (GRCm39)	missense	possibly damaging	0.94
R0321:Oprm1	UTSW	10	6,779,183 (GRCm39)	missense	probably damaging	1.00
R0629:Oprm1	UTSW	10	6,782,604 (GRCm39)	splice site	probably null
R0730:Oprm1	UTSW	10	6,782,652 (GRCm39)	intron	probably benign
R1542:Oprm1	UTSW	10	6,738,960 (GRCm39)	missense	probably damaging	1.00
R1743:Oprm1	UTSW	10	6,780,105 (GRCm39)	missense	probably damaging	0.99
R1874:Oprm1	UTSW	10	6,739,035 (GRCm39)	missense	probably benign	0.17
R2864:Oprm1	UTSW	10	6,744,226 (GRCm39)	splice site	probably null
R2964:Oprm1	UTSW	10	6,738,914 (GRCm39)	missense	probably damaging	0.98
R3792:Oprm1	UTSW	10	6,789,544 (GRCm39)	missense	probably benign	0.00
R4008:Oprm1	UTSW	10	6,782,520 (GRCm39)	missense	probably benign
R4088:Oprm1	UTSW	10	6,780,234 (GRCm39)	missense	probably damaging	1.00
R4724:Oprm1	UTSW	10	6,708,656 (GRCm39)	nonsense	probably null
R4812:Oprm1	UTSW	10	6,782,698 (GRCm39)	intron	probably benign
R4822:Oprm1	UTSW	10	6,779,036 (GRCm39)	missense	probably damaging	0.99
R4855:Oprm1	UTSW	10	6,788,468 (GRCm39)	missense	probably benign	0.01
R5072:Oprm1	UTSW	10	6,782,550 (GRCm39)	missense	probably benign	0.15
R5768:Oprm1	UTSW	10	6,739,026 (GRCm39)	missense	probably damaging	1.00
R5770:Oprm1	UTSW	10	6,739,026 (GRCm39)	missense	probably damaging	1.00
R5995:Oprm1	UTSW	10	6,782,520 (GRCm39)	missense	probably benign
R6327:Oprm1	UTSW	10	6,780,063 (GRCm39)	missense	probably damaging	0.99
R7135:Oprm1	UTSW	10	6,780,203 (GRCm39)	missense	possibly damaging	0.77
R7413:Oprm1	UTSW	10	6,778,919 (GRCm39)	missense	probably damaging	1.00
R7455:Oprm1	UTSW	10	6,780,204 (GRCm39)	missense	probably damaging	1.00
R8192:Oprm1	UTSW	10	6,788,417 (GRCm39)	missense	probably benign	0.04
R8210:Oprm1	UTSW	10	6,780,442 (GRCm39)	missense	probably benign	0.02
R8945:Oprm1	UTSW	10	6,782,644 (GRCm39)	intron	probably benign
R9054:Oprm1	UTSW	10	6,773,914 (GRCm39)	intron	probably benign
R9723:Oprm1	UTSW	10	6,788,514 (GRCm39)	missense	possibly damaging	0.66
R9726:Oprm1	UTSW	10	6,929,694 (GRCm39)	missense	probably benign	0.00
X0066:Oprm1	UTSW	10	6,780,462 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- AAGTAATTTCTTTGCTGTCCTTCGG -3'
(R):5'- AGGCCCCTGTTAACCCTTAC -3'

Sequencing Primer
(F):5'- GACTGCCACCAACATCTACATTTTC -3'
(R):5'- ACCCTGCCTGTATTTTGTGG -3'

Posted On

2015-04-30