Mutagenetix > Incidental Mutation

Incidental Mutation 'R4052:Rif1'

314184

Institutional Source

Beutler Lab

Gene Symbol

Rif1

Ensembl Gene

ENSMUSG00000036202

Gene Name

replication timing regulatory factor 1

Synonyms

6530403D07Rik, 5730435J01Rik, D2Ertd145e

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4052 ()

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

52072832-52122383 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 52098471 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 741 (K741*)

Ref Sequence

ENSEMBL: ENSMUSP00000108313 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069794] [ENSMUST00000112693]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000069794
AA Change: K741*

SMART Domains

Protein: ENSMUSP00000064155
Gene: ENSMUSG00000036202
AA Change: K741*

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	22	368	3.3e-78	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000112693
AA Change: K741*

SMART Domains

Protein: ENSMUSP00000108313
Gene: ENSMUSG00000036202
AA Change: K741*

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	18	381	1.4e-85	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125116

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145130

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.8%
20x: 93.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality. Mice homozygous for a gene trap allele exhibit embryonic and postnatal lethality, reduced fertility, and decreased cell proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110070M22Rik	T	G	13: 119,488,202 (GRCm38)		probably benign	Het
Abca8b	G	A	11: 109,981,725 (GRCm38)	Q17*	probably null	Het
Abcc6	A	C	7: 45,986,563 (GRCm38)	L1020R	probably damaging	Het
Ace2	A	G	X: 164,169,585 (GRCm38)	I110V	probably benign	Het
Adam29	T	C	8: 55,872,282 (GRCm38)	Y379C	probably damaging	Het
Apol10a	A	T	15: 77,488,985 (GRCm38)	I274L	probably benign	Het
BC005537	T	A	13: 24,809,898 (GRCm38)	F119I	possibly damaging	Het
Crygs	C	T	16: 22,805,551 (GRCm38)	G102D	possibly damaging	Het
Cts3	T	A	13: 61,568,721 (GRCm38)	I34L	probably benign	Het
Cyp4a30b	A	G	4: 115,454,342 (GRCm38)	D69G	probably benign	Het
Dclk2	A	G	3: 86,830,822 (GRCm38)		probably null	Het
Dhx30	A	G	9: 110,100,821 (GRCm38)	V69A	possibly damaging	Het
Dis3	T	G	14: 99,095,316 (GRCm38)	I227L	probably benign	Het
Dock3	A	G	9: 106,973,796 (GRCm38)	S836P	probably damaging	Het
Efhc2	T	C	X: 17,230,550 (GRCm38)	N186S	possibly damaging	Het
Erap1	A	G	13: 74,675,340 (GRCm38)	N831S	probably benign	Het
Gimap3	T	A	6: 48,766,513 (GRCm38)	T3S	possibly damaging	Het
Gpr82	C	T	X: 13,665,659 (GRCm38)	P149S	probably damaging	Het
H3f3a	C	T	1: 180,803,138 (GRCm38)	R117H	probably benign	Het
Hcn2	A	C	10: 79,733,687 (GRCm38)		probably null	Het
Helz2	A	G	2: 181,240,475 (GRCm38)	L175P	probably damaging	Het
Il1f8	T	C	2: 24,159,832 (GRCm38)	F152L	probably damaging	Het
Ino80b	G	C	6: 83,122,333 (GRCm38)	P178R	probably damaging	Het
Ldlrad3	C	T	2: 101,953,162 (GRCm38)	D240N	probably damaging	Het
Lef1	A	G	3: 131,194,689 (GRCm38)	N236D	probably damaging	Het
Macf1	A	G	4: 123,472,017 (GRCm38)	S1419P	probably benign	Het
Me2	T	C	18: 73,791,085 (GRCm38)	K352R	probably benign	Het
Ncapd3	T	A	9: 27,089,383 (GRCm38)		probably null	Het
Or52n3	A	G	7: 104,881,603 (GRCm38)	T299A	probably damaging	Het
P2rx7	T	C	5: 122,666,277 (GRCm38)	F293S	probably damaging	Het
Pabpc1l	T	C	2: 164,043,613 (GRCm38)	W429R	probably benign	Het
Parp14	T	C	16: 35,858,401 (GRCm38)	E399G	probably benign	Het
Pde6h	A	G	6: 136,959,268 (GRCm38)	D3G	unknown	Het
Pfkfb1	A	T	X: 150,622,188 (GRCm38)	D208V	possibly damaging	Het
Rasgrp3	T	C	17: 75,496,968 (GRCm38)	F89L	probably damaging	Het
Rnase4	A	G	14: 51,105,005 (GRCm38)	K62R	probably benign	Het
Rnf207	C	A	4: 152,311,437 (GRCm38)	Q533H	probably benign	Het
Slc24a2	A	G	4: 87,227,205 (GRCm38)	V204A	probably damaging	Het
Spry2	A	T	14: 105,893,201 (GRCm38)	C184S	probably damaging	Het
Sulf2	T	C	2: 166,094,590 (GRCm38)	Y152C	probably damaging	Het
Thrap3	G	A	4: 126,176,219 (GRCm38)	A625V	probably damaging	Het
Tmem39a	T	A	16: 38,586,288 (GRCm38)	V329D	probably damaging	Het
Trav7-3	A	G	14: 53,443,746 (GRCm38)	T82A	probably benign	Het
Trim30b	T	A	7: 104,357,478 (GRCm38)	Q57L	possibly damaging	Het
Uggt1	A	G	1: 36,164,489 (GRCm38)	V1020A	probably damaging	Het
Zfp941	T	G	7: 140,812,427 (GRCm38)	K340Q	possibly damaging	Het

Other mutations in Rif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Rif1	APN	2	52,121,007 (GRCm38)	missense	probably damaging	0.96
IGL00711:Rif1	APN	2	52,111,070 (GRCm38)	missense	probably benign	0.00
IGL00721:Rif1	APN	2	52,119,117 (GRCm38)	missense	probably damaging	1.00
IGL01085:Rif1	APN	2	52,085,140 (GRCm38)	missense	possibly damaging	0.71
IGL01093:Rif1	APN	2	52,095,948 (GRCm38)	missense	probably damaging	1.00
IGL01107:Rif1	APN	2	52,111,303 (GRCm38)	missense	probably benign	0.00
IGL01138:Rif1	APN	2	52,111,522 (GRCm38)	missense	probably damaging	1.00
IGL01844:Rif1	APN	2	52,112,543 (GRCm38)	missense	probably benign	0.07
IGL02441:Rif1	APN	2	52,105,515 (GRCm38)	missense	probably benign	0.00
IGL02448:Rif1	APN	2	52,116,696 (GRCm38)	missense	probably damaging	0.99
IGL02563:Rif1	APN	2	52,077,065 (GRCm38)	missense	probably damaging	1.00
IGL02704:Rif1	APN	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
IGL02946:Rif1	APN	2	52,110,125 (GRCm38)	nonsense	probably null
IGL03060:Rif1	APN	2	52,112,137 (GRCm38)	missense	probably damaging	0.97
IGL03206:Rif1	APN	2	52,103,622 (GRCm38)	missense	probably damaging	1.00
IGL03263:Rif1	APN	2	52,090,261 (GRCm38)	missense	probably damaging	0.99
IGL03267:Rif1	APN	2	52,076,988 (GRCm38)	missense	possibly damaging	0.94
IGL03280:Rif1	APN	2	52,112,599 (GRCm38)	missense	probably benign	0.32
hifi	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
nietzsche	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
PIT4305001:Rif1	UTSW	2	52,111,958 (GRCm38)	missense
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0104:Rif1	UTSW	2	52,110,092 (GRCm38)	missense	possibly damaging	0.77
R0268:Rif1	UTSW	2	52,090,286 (GRCm38)	critical splice donor site	probably null
R0276:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0278:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0288:Rif1	UTSW	2	52,110,013 (GRCm38)	missense	probably damaging	1.00
R0314:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0345:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0346:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0383:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R0384:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0387:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0388:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0456:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0477:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0505:Rif1	UTSW	2	52,110,737 (GRCm38)	missense	probably damaging	0.99
R0510:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0511:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0633:Rif1	UTSW	2	52,112,563 (GRCm38)	missense	probably benign	0.00
R0637:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0638:Rif1	UTSW	2	52,111,588 (GRCm38)	missense	probably benign	0.12
R0666:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0675:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0707:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0726:Rif1	UTSW	2	52,110,353 (GRCm38)	missense	possibly damaging	0.52
R0743:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0744:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0938:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0939:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0940:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0941:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0942:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0943:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1006:Rif1	UTSW	2	52,085,029 (GRCm38)	missense	probably damaging	0.99
R1052:Rif1	UTSW	2	52,111,562 (GRCm38)	missense	probably benign	0.01
R1061:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1175:Rif1	UTSW	2	52,107,628 (GRCm38)	unclassified	probably benign
R1183:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1184:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1271:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1332:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1336:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1351:Rif1	UTSW	2	52,111,555 (GRCm38)	missense	possibly damaging	0.74
R1517:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1527:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1560:Rif1	UTSW	2	52,111,131 (GRCm38)	missense	probably damaging	1.00
R1563:Rif1	UTSW	2	52,073,223 (GRCm38)	missense	probably damaging	0.99
R1571:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1625:Rif1	UTSW	2	52,103,640 (GRCm38)	missense	probably benign	0.25
R1679:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1689:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1731:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1744:Rif1	UTSW	2	52,112,392 (GRCm38)	missense	possibly damaging	0.56
R1746:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1748:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1831:Rif1	UTSW	2	52,078,495 (GRCm38)	nonsense	probably null
R1902:Rif1	UTSW	2	52,116,673 (GRCm38)	missense	possibly damaging	0.93
R1964:Rif1	UTSW	2	52,098,409 (GRCm38)	missense	probably benign	0.01
R1978:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2000:Rif1	UTSW	2	52,081,298 (GRCm38)	missense	probably damaging	0.99
R2030:Rif1	UTSW	2	52,092,346 (GRCm38)	missense	probably damaging	1.00
R2056:Rif1	UTSW	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
R2106:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2109:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2125:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2126:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2145:Rif1	UTSW	2	52,111,400 (GRCm38)	missense	possibly damaging	0.63
R2152:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2153:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2213:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2327:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2513:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2516:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2520:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2905:Rif1	UTSW	2	52,098,504 (GRCm38)	missense	probably damaging	0.99
R3005:Rif1	UTSW	2	52,082,764 (GRCm38)	missense	probably damaging	1.00
R3155:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3156:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3429:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3707:Rif1	UTSW	2	52,093,580 (GRCm38)	missense	probably damaging	1.00
R3907:Rif1	UTSW	2	52,112,545 (GRCm38)	missense	probably benign	0.03
R3978:Rif1	UTSW	2	52,116,747 (GRCm38)	critical splice donor site	probably null
R4023:Rif1	UTSW	2	52,121,087 (GRCm38)	missense	probably benign	0.01
R4668:Rif1	UTSW	2	52,111,952 (GRCm38)	missense	probably benign	0.01
R4674:Rif1	UTSW	2	52,106,942 (GRCm38)	missense	probably null	1.00
R4715:Rif1	UTSW	2	52,073,139 (GRCm38)	utr 5 prime	probably benign
R4766:Rif1	UTSW	2	52,098,934 (GRCm38)	missense	probably damaging	1.00
R4783:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4785:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4869:Rif1	UTSW	2	52,093,611 (GRCm38)	intron	probably benign
R4911:Rif1	UTSW	2	52,110,518 (GRCm38)	missense	probably damaging	0.98
R4951:Rif1	UTSW	2	52,084,986 (GRCm38)	splice site	probably null
R5044:Rif1	UTSW	2	52,109,928 (GRCm38)	missense	probably damaging	0.99
R5088:Rif1	UTSW	2	52,092,295 (GRCm38)	missense	possibly damaging	0.91
R5151:Rif1	UTSW	2	52,120,309 (GRCm38)	missense	probably damaging	1.00
R5187:Rif1	UTSW	2	52,081,289 (GRCm38)	missense	probably damaging	1.00
R5222:Rif1	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
R5243:Rif1	UTSW	2	52,111,824 (GRCm38)	missense	possibly damaging	0.86
R5436:Rif1	UTSW	2	52,120,971 (GRCm38)	intron	probably benign
R5476:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
R5496:Rif1	UTSW	2	52,098,916 (GRCm38)	missense	probably damaging	1.00
R5641:Rif1	UTSW	2	52,121,158 (GRCm38)	missense	possibly damaging	0.80
R5883:Rif1	UTSW	2	52,105,639 (GRCm38)	critical splice donor site	probably null
R5987:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5990:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5992:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6019:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6020:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6255:Rif1	UTSW	2	52,085,053 (GRCm38)	missense	probably damaging	1.00
R6342:Rif1	UTSW	2	52,119,156 (GRCm38)	missense	probably damaging	0.97
R6364:Rif1	UTSW	2	52,107,669 (GRCm38)	missense	probably damaging	0.97
R6747:Rif1	UTSW	2	52,078,263 (GRCm38)	splice site	probably null
R6928:Rif1	UTSW	2	52,095,961 (GRCm38)	missense	probably damaging	1.00
R6954:Rif1	UTSW	2	52,112,691 (GRCm38)	missense	probably benign	0.00
R7003:Rif1	UTSW	2	52,076,989 (GRCm38)	missense	probably benign	0.06
R7310:Rif1	UTSW	2	52,105,619 (GRCm38)	missense	probably benign	0.12
R7549:Rif1	UTSW	2	52,078,507 (GRCm38)	missense	possibly damaging	0.52
R7603:Rif1	UTSW	2	52,076,175 (GRCm38)	missense	probably damaging	1.00
R7673:Rif1	UTSW	2	52,088,654 (GRCm38)	missense	probably damaging	1.00
R7741:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R7777:Rif1	UTSW	2	52,116,356 (GRCm38)	missense	probably benign	0.00
R7910:Rif1	UTSW	2	52,078,387 (GRCm38)	nonsense	probably null
R7962:Rif1	UTSW	2	52,074,276 (GRCm38)	missense	probably damaging	1.00
R8264:Rif1	UTSW	2	52,090,278 (GRCm38)	missense	noncoding transcript
R8390:Rif1	UTSW	2	52,110,923 (GRCm38)	missense	probably damaging	1.00
R8479:Rif1	UTSW	2	52,112,551 (GRCm38)	missense	possibly damaging	0.52
R8490:Rif1	UTSW	2	52,110,999 (GRCm38)	missense	probably damaging	0.96
R8762:Rif1	UTSW	2	52,111,730 (GRCm38)	missense
R8785:Rif1	UTSW	2	52,110,481 (GRCm38)	missense	probably benign	0.06
R8890:Rif1	UTSW	2	52,098,863 (GRCm38)	missense	probably damaging	0.99
R9081:Rif1	UTSW	2	52,110,977 (GRCm38)	missense	probably damaging	0.99
R9225:Rif1	UTSW	2	52,111,850 (GRCm38)	missense	probably benign	0.22
R9284:Rif1	UTSW	2	52,108,552 (GRCm38)	missense	probably benign	0.00
R9300:Rif1	UTSW	2	52,111,139 (GRCm38)	missense	probably damaging	1.00
R9366:Rif1	UTSW	2	52,120,344 (GRCm38)	missense
R9477:Rif1	UTSW	2	52,111,330 (GRCm38)	missense	probably benign	0.02
R9522:Rif1	UTSW	2	52,081,299 (GRCm38)	missense	probably damaging	1.00
R9573:Rif1	UTSW	2	52,110,454 (GRCm38)	missense	probably benign	0.29
R9630:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
X0064:Rif1	UTSW	2	52,094,633 (GRCm38)	missense	probably damaging	0.96
X0064:Rif1	UTSW	2	52,074,315 (GRCm38)	missense	probably benign	0.00
Z1177:Rif1	UTSW	2	52,088,648 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTCCCTCTGACAAGTGAAGTG -3'
(R):5'- GCTCTATGTAGCACAAGGAAGG -3'

Sequencing Primer
(F):5'- CCTCTGACAAGTGAAGTGTGTCAC -3'
(R):5'- CAGAAGTGTCAATAAACCGTC -3'

Posted On

2015-04-30