Mutagenetix > Incidental Mutation

Incidental Mutation 'R4058:Tns3'

314352

Institutional Source

Beutler Lab

Gene Symbol

Tns3

Ensembl Gene

ENSMUSG00000020422

Gene Name

tensin 3

Synonyms

F830010I22Rik, Tens1, TEM6

MMRRC Submission

040969-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.327) question?

Stock #

R4058 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

8381652-8614681 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 8442275 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 696 (D696G)

Ref Sequence

ENSEMBL: ENSMUSP00000020695 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020695]

AlphaFold

Q5SSZ5

Predicted Effect

probably damaging
Transcript: ENSMUST00000020695
AA Change: D696G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000020695
Gene: ENSMUSG00000020422
AA Change: D696G

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	1	171	5e-28	SMART
PTEN_C2	173	300	1.15e-48	SMART
low complexity region	854	864	N/A	INTRINSIC
low complexity region	1102	1126	N/A	INTRINSIC
SH2	1165	1268	1.32e-18	SMART
PTB	1301	1438	3.14e-24	SMART

Meta Mutation Damage Score

0.0942

Coding Region Coverage

1x: 99.1%
3x: 98.5%
10x: 97.2%
20x: 94.8%

Validation Efficiency

88% (38/43)

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit one third postnatal lethality, reduced body weight, growth retardation, smaller digestive tracts with defects in villi and enterocyte differentiation, abnormal lung morphology, and thinner bones with decreased chondrocyte proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930453N24Rik	A	G	16: 64,586,821 (GRCm39)	V301A	probably benign	Het
Adam15	A	G	3: 89,254,362 (GRCm39)	V145A	possibly damaging	Het
Anxa4	C	T	6: 86,734,800 (GRCm39)		probably null	Het
Aqp9	C	A	9: 71,037,726 (GRCm39)	V184L	probably benign	Het
Atp13a3	C	A	16: 30,173,064 (GRCm39)	C271F	possibly damaging	Het
B4galt3	C	A	1: 171,101,613 (GRCm39)	H196N	probably damaging	Het
Cldn34c4	C	A	X: 126,629,060 (GRCm39)	V137F	probably benign	Het
Cngb1	T	C	8: 95,994,282 (GRCm39)	E163G	probably benign	Het
Dync1i1	A	G	6: 5,769,764 (GRCm39)	D113G	probably damaging	Het
Etl4	T	A	2: 20,810,830 (GRCm39)	V971D	possibly damaging	Het
Gys1	A	G	7: 45,097,810 (GRCm39)		probably benign	Het
H13	C	G	2: 152,533,794 (GRCm39)	P227R	probably damaging	Het
Ift22	C	A	5: 136,940,717 (GRCm39)	P84Q	unknown	Het
Igfn1	A	G	1: 135,897,494 (GRCm39)	V1024A	probably benign	Het
Kdm8	T	A	7: 125,055,666 (GRCm39)	Y65N	probably damaging	Het
Lbp	T	A	2: 158,166,550 (GRCm39)	V368E	probably damaging	Het
Lmo2	T	C	2: 103,811,407 (GRCm39)	Y147H	probably damaging	Het
Megf6	T	C	4: 154,326,989 (GRCm39)		probably benign	Het
Mettl13	G	T	1: 162,373,755 (GRCm39)	H165Q	probably damaging	Het
Mitd1	C	T	1: 37,920,107 (GRCm39)	S167N	probably benign	Het
Mon2	A	G	10: 122,838,724 (GRCm39)	V1593A	probably benign	Het
Nkx3-2	T	A	5: 41,919,406 (GRCm39)	E194V	possibly damaging	Het
Nup210	A	T	6: 91,037,602 (GRCm39)	V757D	probably benign	Het
Opcml	A	G	9: 28,812,884 (GRCm39)	Y192C	probably damaging	Het
Or10ak7	T	C	4: 118,791,880 (GRCm39)	D53G	probably damaging	Het
Or5m8	T	A	2: 85,822,576 (GRCm39)	S138R	possibly damaging	Het
Pcdha2	A	G	18: 37,072,935 (GRCm39)	S189G	probably benign	Het
Pkd2l2	T	C	18: 34,561,245 (GRCm39)	F418L	probably benign	Het
Plekhg1	A	G	10: 3,907,087 (GRCm39)	D668G	probably damaging	Het
Prep	G	A	10: 45,034,467 (GRCm39)	V660M	probably benign	Het
Qrfprl	T	A	6: 65,358,525 (GRCm39)	I83N	probably damaging	Het
Rgs8	A	G	1: 153,566,742 (GRCm39)	T98A	probably null	Het
Rhbdd1	A	G	1: 82,348,102 (GRCm39)	N235D	possibly damaging	Het
Rin2	C	T	2: 145,702,366 (GRCm39)	T354I	probably benign	Het
Sgo2b	A	T	8: 64,379,981 (GRCm39)	D950E	probably damaging	Het
Slc1a5	T	C	7: 16,529,778 (GRCm39)	V399A	probably damaging	Het
Spag16	A	T	1: 69,892,487 (GRCm39)	Q89H	probably damaging	Het
Spta1	T	C	1: 174,068,703 (GRCm39)	W2168R	probably damaging	Het
Taok1	T	A	11: 77,440,264 (GRCm39)	K581M	probably benign	Het
Tspan8	C	T	10: 115,671,187 (GRCm39)	R115*	probably null	Het
Txnrd1	A	G	10: 82,721,114 (GRCm39)	E510G	probably benign	Het
Usp45	T	C	4: 21,810,746 (GRCm39)	I314T	probably damaging	Het
Vmn2r15	T	A	5: 109,441,312 (GRCm39)	H182L	probably damaging	Het
Vmn2r76	A	C	7: 85,879,508 (GRCm39)	M264R	probably benign	Het

Other mutations in Tns3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Tns3	APN	11	8,401,066 (GRCm39)	missense	probably benign	0.42
IGL00822:Tns3	APN	11	8,393,976 (GRCm39)	missense	probably damaging	0.99
IGL01075:Tns3	APN	11	8,428,399 (GRCm39)	missense	probably benign	0.45
IGL01286:Tns3	APN	11	8,442,617 (GRCm39)	missense	probably benign	0.01
IGL01680:Tns3	APN	11	8,498,937 (GRCm39)	missense	probably damaging	1.00
IGL01687:Tns3	APN	11	8,442,798 (GRCm39)	missense	probably damaging	1.00
IGL01734:Tns3	APN	11	8,469,192 (GRCm39)	splice site	probably benign
IGL01844:Tns3	APN	11	8,387,177 (GRCm39)	missense	possibly damaging	0.58
IGL01984:Tns3	APN	11	8,498,992 (GRCm39)	nonsense	probably null
IGL02137:Tns3	APN	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
IGL02273:Tns3	APN	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
IGL02623:Tns3	APN	11	8,387,141 (GRCm39)	missense	probably damaging	1.00
IGL02697:Tns3	APN	11	8,442,346 (GRCm39)	missense	probably benign	0.00
IGL02829:Tns3	APN	11	8,469,564 (GRCm39)	missense	probably damaging	1.00
ANU74:Tns3	UTSW	11	8,442,149 (GRCm39)	missense	probably benign	0.38
R0020:Tns3	UTSW	11	8,495,227 (GRCm39)	critical splice donor site	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0064:Tns3	UTSW	11	8,385,856 (GRCm39)	nonsense	probably null
R0370:Tns3	UTSW	11	8,395,730 (GRCm39)	missense	possibly damaging	0.80
R0388:Tns3	UTSW	11	8,395,703 (GRCm39)	missense	probably benign	0.07
R0410:Tns3	UTSW	11	8,385,852 (GRCm39)	missense	probably benign	0.02
R0496:Tns3	UTSW	11	8,497,262 (GRCm39)	splice site	probably benign
R0562:Tns3	UTSW	11	8,443,262 (GRCm39)	missense	possibly damaging	0.93
R0626:Tns3	UTSW	11	8,443,121 (GRCm39)	missense	probably benign	0.04
R0736:Tns3	UTSW	11	8,469,474 (GRCm39)	missense	possibly damaging	0.94
R0893:Tns3	UTSW	11	8,443,302 (GRCm39)	missense	probably damaging	1.00
R1367:Tns3	UTSW	11	8,398,704 (GRCm39)	missense	probably benign	0.01
R1386:Tns3	UTSW	11	8,468,261 (GRCm39)	missense	probably benign	0.02
R1975:Tns3	UTSW	11	8,385,738 (GRCm39)	missense	probably benign	0.04
R2205:Tns3	UTSW	11	8,481,719 (GRCm39)	missense	probably damaging	1.00
R2319:Tns3	UTSW	11	8,491,200 (GRCm39)	missense	probably damaging	1.00
R2830:Tns3	UTSW	11	8,385,870 (GRCm39)	missense	probably damaging	1.00
R3720:Tns3	UTSW	11	8,442,999 (GRCm39)	missense	probably damaging	1.00
R3765:Tns3	UTSW	11	8,401,133 (GRCm39)	missense	probably benign	0.00
R3817:Tns3	UTSW	11	8,384,619 (GRCm39)	missense	probably damaging	1.00
R4599:Tns3	UTSW	11	8,481,747 (GRCm39)	missense	probably damaging	1.00
R4631:Tns3	UTSW	11	8,401,119 (GRCm39)	missense	probably benign	0.30
R4731:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4732:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R4733:Tns3	UTSW	11	8,400,986 (GRCm39)	missense	probably benign	0.28
R5472:Tns3	UTSW	11	8,401,092 (GRCm39)	missense	probably benign
R5749:Tns3	UTSW	11	8,401,177 (GRCm39)	missense	probably benign	0.01
R5807:Tns3	UTSW	11	8,443,211 (GRCm39)	missense	probably damaging	1.00
R5844:Tns3	UTSW	11	8,384,580 (GRCm39)	missense	probably damaging	1.00
R5942:Tns3	UTSW	11	8,385,860 (GRCm39)	missense	probably damaging	1.00
R5982:Tns3	UTSW	11	8,442,245 (GRCm39)	missense	probably damaging	0.99
R6025:Tns3	UTSW	11	8,442,578 (GRCm39)	missense	possibly damaging	0.93
R6266:Tns3	UTSW	11	8,442,987 (GRCm39)	missense	probably damaging	1.00
R6322:Tns3	UTSW	11	8,442,147 (GRCm39)	missense	probably benign	0.01
R6536:Tns3	UTSW	11	8,384,531 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,058 (GRCm39)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,499,057 (GRCm39)	missense	probably damaging	1.00
R6864:Tns3	UTSW	11	8,443,196 (GRCm39)	missense	probably damaging	1.00
R6897:Tns3	UTSW	11	8,481,743 (GRCm39)	missense	probably damaging	1.00
R7108:Tns3	UTSW	11	8,387,251 (GRCm39)	missense	probably benign	0.00
R7443:Tns3	UTSW	11	8,401,442 (GRCm39)	missense	probably benign	0.01
R7459:Tns3	UTSW	11	8,442,793 (GRCm39)	missense	probably benign	0.16
R7474:Tns3	UTSW	11	8,480,894 (GRCm39)	missense	probably damaging	1.00
R7576:Tns3	UTSW	11	8,491,192 (GRCm39)	missense	possibly damaging	0.78
R7979:Tns3	UTSW	11	8,442,701 (GRCm39)	missense	probably benign	0.01
R8055:Tns3	UTSW	11	8,495,343 (GRCm39)	missense	probably damaging	1.00
R8057:Tns3	UTSW	11	8,442,773 (GRCm39)	missense	probably benign
R8077:Tns3	UTSW	11	8,395,667 (GRCm39)	missense	probably damaging	1.00
R8518:Tns3	UTSW	11	8,442,971 (GRCm39)	missense	probably damaging	0.96
R8523:Tns3	UTSW	11	8,398,779 (GRCm39)	missense	probably damaging	1.00
R8790:Tns3	UTSW	11	8,468,273 (GRCm39)	missense	probably damaging	0.99
R9228:Tns3	UTSW	11	8,400,094 (GRCm39)	missense	probably damaging	1.00
R9374:Tns3	UTSW	11	8,442,606 (GRCm39)	missense	probably damaging	1.00
R9476:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9510:Tns3	UTSW	11	8,395,702 (GRCm39)	missense	probably damaging	0.99
R9594:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9595:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9596:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9624:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
R9629:Tns3	UTSW	11	8,401,142 (GRCm39)	missense	possibly damaging	0.79
T0975:Tns3	UTSW	11	8,401,146 (GRCm39)	missense	probably benign	0.00
T0975:Tns3	UTSW	11	8,499,100 (GRCm39)	start gained	probably benign
T0975:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
X0005:Tns3	UTSW	11	8,429,518 (GRCm39)	missense	probably benign
X0005:Tns3	UTSW	11	8,401,224 (GRCm39)	missense	probably benign	0.00
Z1177:Tns3	UTSW	11	8,401,014 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGCTGCATAGATACTTCCTACCTG -3'
(R):5'- AGCAGAGTGGCTGTTCAGAG -3'

Sequencing Primer
(F):5'- ATCAGCTCACCTTGCCGG -3'
(R):5'- CTGTTCAGAGAGGCATCAGC -3'

Posted On

2015-04-30