Mutagenetix > Incidental Mutation

Incidental Mutation 'R4116:Zfp773'

314632

Institutional Source

Beutler Lab

Gene Symbol

Zfp773

Ensembl Gene

ENSMUSG00000063535

Gene Name

zinc finger protein 773

Synonyms

2810409K11Rik

MMRRC Submission

040859-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R4116 (G1)

Quality Score

109

Status

Not validated

Chromosome

Chromosomal Location

7133677-7139754 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

AGCTGCTGCTGCTGCTGCTGCTGCTGC to AGCTGCTGCTGCTGCTGCTGCTGC at 7136092 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000147639 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032622] [ENSMUST00000211240]

AlphaFold

Q9CZ29

Predicted Effect

probably benign
Transcript: ENSMUST00000032622

SMART Domains

Protein: ENSMUSP00000032622
Gene: ENSMUSG00000063535

Domain	Start	End	E-Value	Type
KRAB	75	134	6.82e-8	SMART
ZnF_C2H2	241	263	1.31e0	SMART
ZnF_C2H2	269	291	1.5e-4	SMART
ZnF_C2H2	297	319	4.17e-3	SMART
ZnF_C2H2	325	347	2.05e-2	SMART
ZnF_C2H2	353	375	2.24e-3	SMART
ZnF_C2H2	381	403	8.81e-2	SMART
ZnF_C2H2	409	431	7.26e-3	SMART
ZnF_C2H2	437	459	7.26e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000211240

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.6%
20x: 96.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	A	T	17: 46,634,817 (GRCm39)	F395L	possibly damaging	Het
Amigo1	C	T	3: 108,095,761 (GRCm39)	T420I	probably damaging	Het
Ankrd34c	A	G	9: 89,611,927 (GRCm39)	L138P	probably damaging	Het
Cald1	C	T	6: 34,722,654 (GRCm39)	R107C	probably damaging	Het
Cast	C	T	13: 74,872,956 (GRCm39)	E444K	probably damaging	Het
Ccdc121rt2	A	G	5: 112,598,377 (GRCm39)	E308G	probably damaging	Het
Crybg1	T	G	10: 43,875,158 (GRCm39)	N650T	possibly damaging	Het
Cyp19a1	T	A	9: 54,076,025 (GRCm39)	R276S	possibly damaging	Het
Cyp2c66	A	G	19: 39,165,003 (GRCm39)	D328G	possibly damaging	Het
Hectd1	A	T	12: 51,815,506 (GRCm39)	L1527*	probably null	Het
Hoxd13	C	A	2: 74,498,832 (GRCm39)	A60E	possibly damaging	Het
Igkv3-7	G	T	6: 70,584,923 (GRCm39)	G88C	probably damaging	Het
Kcnk12	GGCATCGC	GGCATCGCATCGC	17: 88,053,584 (GRCm39)		probably null	Het
Klhl30	C	A	1: 91,281,830 (GRCm39)	Q144K	probably benign	Het
Kprp	A	T	3: 92,731,275 (GRCm39)	S592T	probably damaging	Het
Man2c1	T	C	9: 57,047,589 (GRCm39)		probably null	Het
Mbtps1	A	T	8: 120,268,391 (GRCm39)	M260K	probably benign	Het
Morc3	G	A	16: 93,670,227 (GRCm39)	D801N	probably benign	Het
Mpped1	A	G	15: 83,680,910 (GRCm39)		probably benign	Het
Mtcl1	T	C	17: 66,673,476 (GRCm39)	T767A	probably benign	Het
Nek5	G	A	8: 22,601,178 (GRCm39)	T181M	probably damaging	Het
Nomo1	T	C	7: 45,683,320 (GRCm39)	I22T	probably benign	Het
Or7g27	T	G	9: 19,249,940 (GRCm39)	F61L	probably benign	Het
Plin4	A	G	17: 56,409,113 (GRCm39)	V1369A	probably benign	Het
Polr3c	A	T	3: 96,622,560 (GRCm39)	F365Y	probably damaging	Het
Sec22a	A	G	16: 35,139,202 (GRCm39)	F232S	probably damaging	Het
Sema4f	G	A	6: 82,894,887 (GRCm39)	T436M	probably benign	Het
Sf3a2	A	G	10: 80,637,175 (GRCm39)	T37A	probably damaging	Het
Slc1a6	A	G	10: 78,623,723 (GRCm39)	M41V	probably benign	Het
Sptbn4	C	T	7: 27,090,995 (GRCm39)	E1399K	probably damaging	Het
Sspo	T	C	6: 48,433,928 (GRCm39)	L911P	probably damaging	Het
Sult2a2	A	T	7: 13,468,708 (GRCm39)	Q58L	probably benign	Het
Syne2	A	T	12: 75,977,853 (GRCm39)	I1433F	probably damaging	Het
Synm	C	T	7: 67,384,405 (GRCm39)	V644I	possibly damaging	Het
Tbc1d5	A	G	17: 51,227,615 (GRCm39)	L210P	probably damaging	Het
Trak1	T	C	9: 121,277,909 (GRCm39)	Y229H	probably damaging	Het
Unc5b	G	T	10: 60,610,479 (GRCm39)	T446K	probably damaging	Het
Wdr95	C	A	5: 149,521,040 (GRCm39)	D604E	probably benign	Het

Other mutations in Zfp773

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00764:Zfp773	APN	7	7,135,683 (GRCm39)	missense	probably damaging	1.00
IGL00780:Zfp773	APN	7	7,136,113 (GRCm39)	missense	probably benign	0.00
IGL01348:Zfp773	APN	7	7,138,314 (GRCm39)	missense	possibly damaging	0.93
IGL02224:Zfp773	APN	7	7,135,975 (GRCm39)	missense	probably benign	0.00
IGL02447:Zfp773	APN	7	7,139,655 (GRCm39)	utr 5 prime	probably benign
IGL02869:Zfp773	APN	7	7,137,232 (GRCm39)	missense	probably benign	0.22
R0505:Zfp773	UTSW	7	7,136,023 (GRCm39)	missense	probably benign	0.03
R0585:Zfp773	UTSW	7	7,135,574 (GRCm39)	missense	probably benign	0.21
R0804:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R0846:Zfp773	UTSW	7	7,135,691 (GRCm39)	missense	probably damaging	1.00
R1179:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R2847:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R3841:Zfp773	UTSW	7	7,135,390 (GRCm39)	missense	possibly damaging	0.92
R4638:Zfp773	UTSW	7	7,138,335 (GRCm39)	missense	probably damaging	1.00
R5126:Zfp773	UTSW	7	7,139,623 (GRCm39)	missense	unknown
R6142:Zfp773	UTSW	7	7,135,481 (GRCm39)	missense	probably benign	0.00
R7072:Zfp773	UTSW	7	7,135,874 (GRCm39)	missense	probably benign	0.15
R7232:Zfp773	UTSW	7	7,135,984 (GRCm39)	missense	probably benign	0.14
R7748:Zfp773	UTSW	7	7,135,907 (GRCm39)	missense	probably benign	0.04
R7888:Zfp773	UTSW	7	7,135,978 (GRCm39)	missense	probably benign	0.00
R8681:Zfp773	UTSW	7	7,139,482 (GRCm39)	missense	possibly damaging	0.70
R8784:Zfp773	UTSW	7	7,135,570 (GRCm39)	missense	probably benign	0.19
R8946:Zfp773	UTSW	7	7,135,469 (GRCm39)	missense	possibly damaging	0.82
R9056:Zfp773	UTSW	7	7,135,989 (GRCm39)	missense	probably damaging	0.99
R9154:Zfp773	UTSW	7	7,138,302 (GRCm39)	missense	probably damaging	0.98
R9295:Zfp773	UTSW	7	7,135,694 (GRCm39)	missense	probably benign	0.06
RF007:Zfp773	UTSW	7	7,135,689 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GCCACAGTCGTAACATTTGC -3'
(R):5'- AGAAATCTCGGGTGTTGATTGC -3'

Sequencing Primer
(F):5'- GCCACAGTCGTAACATTTGCAATTC -3'
(R):5'- CTTGTGTTTGTTGCCACTTCAAAG -3'

Posted On

2015-05-14