Mutagenetix > Incidental Mutations

Incidental Mutation 'R4131:Try4'

314671

Institutional Source

Beutler Lab

Gene Symbol

Try4

Ensembl Gene

ENSMUSG00000054106

Gene Name

trypsin 4

Synonyms

0910001B19Rik, Td

MMRRC Submission

040994-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4131 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

41302269-41305532 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 41305401 bp

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 218 (Y218*)

Ref Sequence

ENSEMBL: ENSMUSP00000031913 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031913]

Predicted Effect

probably null
Transcript: ENSMUST00000031913
AA Change: Y218*

SMART Domains

Protein: ENSMUSP00000031913
Gene: ENSMUSG00000054106
AA Change: Y218*

Domain	Start	End	E-Value	Type
low complexity region	3	16	N/A	INTRINSIC
Tryp_SPc	23	239	9.72e-105	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000193013

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.3%
20x: 95.1%

Validation Efficiency

100% (32/32)

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A4gnt	A	G	9: 99,620,618	D277G	possibly damaging	Het
Abca12	A	G	1: 71,319,871		probably null	Het
Adam12	C	T	7: 133,912,924	V345I	probably damaging	Het
Arpp21	T	A	9: 112,155,308		probably benign	Het
Art3	A	T	5: 92,392,562	M55L	probably benign	Het
C1d	C	T	11: 17,264,054		probably benign	Het
Ces1c	A	G	8: 93,100,684	M484T	probably damaging	Het
Cisd3	T	C	11: 97,688,431	V133A	possibly damaging	Het
Clpx	A	G	9: 65,316,655	I230V	possibly damaging	Het
Cmklr1	C	A	5: 113,614,484	R152L	probably damaging	Het
Grm7	G	T	6: 110,646,348	V161F	probably damaging	Het
Heca	A	G	10: 17,902,239	S537P	probably damaging	Het
Igha	C	A	12: 113,258,829		probably benign	Het
Itgb2l	A	G	16: 96,437,389	L70P	probably damaging	Het
Klk1b3	T	A	7: 44,201,687	N181K	probably damaging	Het
Klra2	T	A	6: 131,228,217	R220S	probably benign	Het
Lama2	C	T	10: 27,041,174	V2252I	probably benign	Het
Megf10	G	A	18: 57,180,535	C17Y	probably damaging	Het
Nup35	G	A	2: 80,656,099		probably benign	Het
Olfr503	C	G	7: 108,544,537	S2*	probably null	Het
Olfr884	T	A	9: 38,047,874	Y217*	probably null	Het
Pdzd9	A	T	7: 120,662,869	D123E	possibly damaging	Het
Phactr1	A	T	13: 43,037,477	Q141L	probably damaging	Het
Prelid2	T	G	18: 41,951,159	D6A	possibly damaging	Het
Psmd1	T	C	1: 86,078,700	S263P	probably damaging	Het
Rassf6	C	T	5: 90,609,787	D105N	probably damaging	Het
Ryr3	C	T	2: 112,926,983		probably null	Het
Sema4g	T	A	19: 44,998,919	F501L	probably benign	Het
Ssrp1	A	G	2: 85,044,447	E537G	probably null	Het
Zbtb40	G	T	4: 136,995,396	S790R	probably benign	Het

Other mutations in Try4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01773:Try4	APN	6	41305026	missense	probably damaging	1.00
IGL02216:Try4	APN	6	41305031	missense	probably benign
R0537:Try4	UTSW	6	41304362	missense	probably benign
R0731:Try4	UTSW	6	41304367	missense	probably benign	0.01
R1113:Try4	UTSW	6	41305374	missense	possibly damaging	0.90
R1833:Try4	UTSW	6	41303431	missense	probably damaging	0.98
R2246:Try4	UTSW	6	41305472	missense	possibly damaging	0.80
R4414:Try4	UTSW	6	41304971	missense	possibly damaging	0.84
R5457:Try4	UTSW	6	41303421	missense	probably damaging	1.00
R5707:Try4	UTSW	6	41305043	missense	possibly damaging	0.65
R6023:Try4	UTSW	6	41303421	missense	probably damaging	1.00
R7131:Try4	UTSW	6	41304403	missense	probably benign	0.03
R7783:Try4	UTSW	6	41302295	missense	possibly damaging	0.96
R8051:Try4	UTSW	6	41305062	missense	probably damaging	0.99
RF007:Try4	UTSW	6	41305363	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CATGTCAGCCAGAACAGAGG -3'
(R):5'- GTAGGACGCAAACAACATTTAAACG -3'

Sequencing Primer
(F):5'- CCAGAACAGAGGATGGGCTAACC -3'
(R):5'- TAAGGTATTTCCCACAGACAAAAGG -3'

Posted On

2015-05-14