Mutagenetix > Incidental Mutation

Incidental Mutation 'R4133:Or9s15'

314722

Institutional Source

Beutler Lab

Gene Symbol

Or9s15

Ensembl Gene

ENSMUSG00000062497

Gene Name

olfactory receptor family 9 subfamily S member 15

Synonyms

Olfr1411, MOR208-3, GA_x6K02T2R7CC-81157497-81156526

MMRRC Submission

041637-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R4133 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

92524243-92525214 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 92524465 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 75 (F75L)

Ref Sequence

ENSEMBL: ENSMUSP00000150254 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073748] [ENSMUST00000190844] [ENSMUST00000216444]

AlphaFold

Q8VFC4

Predicted Effect

probably benign
Transcript: ENSMUST00000073748
AA Change: F75L

PolyPhen 2 Score 0.091 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000073422
Gene: ENSMUSG00000062497
AA Change: F75L

Domain	Start	End	E-Value	Type
low complexity region	16	27	N/A	INTRINSIC
Pfam:7tm_4	38	314	7.4e-53	PFAM
Pfam:7tm_1	48	297	1.2e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000190844
AA Change: F75L

PolyPhen 2 Score 0.091 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000140373
Gene: ENSMUSG00000062497
AA Change: F75L

Domain	Start	End	E-Value	Type
low complexity region	16	27	N/A	INTRINSIC
low complexity region	32	44	N/A	INTRINSIC
Pfam:7tm_1	48	297	6.7e-25	PFAM
Pfam:7tm_4	146	290	2.9e-45	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203299

Predicted Effect

probably benign
Transcript: ENSMUST00000216444
AA Change: F75L

PolyPhen 2 Score 0.091 (Sensitivity: 0.93; Specificity: 0.85)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.4%
20x: 95.5%

Validation Efficiency

97% (77/79)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700036A12Rik	T	A	9: 60,677,280 (GRCm39)		noncoding transcript	Het
Aldh3b1	A	G	19: 3,970,808 (GRCm39)	I184T	probably damaging	Het
Arhgap42	C	T	9: 9,011,300 (GRCm39)		probably benign	Het
Arhgef4	A	G	1: 34,845,185 (GRCm39)	D1463G	probably damaging	Het
Arpc5	A	G	1: 152,644,622 (GRCm39)	T52A	probably benign	Het
Asb6	A	G	2: 30,718,247 (GRCm39)		probably benign	Het
Ash1l	T	C	3: 88,889,567 (GRCm39)	V482A	probably benign	Het
Bop1	T	C	15: 76,338,535 (GRCm39)	N469S	probably benign	Het
Btaf1	T	A	19: 36,939,138 (GRCm39)	N266K	probably benign	Het
Btla	T	C	16: 45,059,661 (GRCm39)	Y122H	probably damaging	Het
Cacna1g	A	G	11: 94,323,370 (GRCm39)	M1278T	probably damaging	Het
Cdc42bpb	A	G	12: 111,287,976 (GRCm39)	S524P	probably benign	Het
Cep78	A	G	19: 15,946,519 (GRCm39)	S438P	probably damaging	Het
Clca4a	T	G	3: 144,675,113 (GRCm39)	E171D	probably benign	Het
Cldn6	A	T	17: 23,900,467 (GRCm39)	I144F	probably damaging	Het
Cpd	G	A	11: 76,705,644 (GRCm39)	Q363*	probably null	Het
Cpn1	G	A	19: 43,974,723 (GRCm39)	P2L	possibly damaging	Het
Ddx31	A	G	2: 28,748,864 (GRCm39)	D264G	probably damaging	Het
Ddx39b	C	T	17: 35,472,065 (GRCm39)	S368L	probably damaging	Het
Ddx60	A	G	8: 62,425,254 (GRCm39)	K681E	probably damaging	Het
Dennd1c	A	C	17: 57,383,980 (GRCm39)	W15G	possibly damaging	Het
Dgkd	T	G	1: 87,869,223 (GRCm39)		probably null	Het
Dnah7c	G	T	1: 46,705,150 (GRCm39)	A2388S	probably benign	Het
Etv4	C	A	11: 101,661,324 (GRCm39)	K442N	probably damaging	Het
Fam234a	T	C	17: 26,432,532 (GRCm39)	D539G	probably damaging	Het
Fancm	A	G	12: 65,167,304 (GRCm39)	T1538A	probably benign	Het
Fbn2	A	T	18: 58,229,034 (GRCm39)	N725K	possibly damaging	Het
Fcer2a	T	A	8: 3,741,130 (GRCm39)	N4I	possibly damaging	Het
Fgd5	T	C	6: 92,046,418 (GRCm39)	V1229A	probably damaging	Het
Garin4	A	T	1: 190,895,205 (GRCm39)	N479K	probably benign	Het
Gm5471	A	T	15: 44,835,297 (GRCm39)		noncoding transcript	Het
Gm5478	T	A	15: 101,553,080 (GRCm39)	I331F	probably damaging	Het
Gm6729	C	A	10: 86,377,030 (GRCm39)		noncoding transcript	Het
Hectd4	G	T	5: 121,415,897 (GRCm39)		probably null	Het
Hspb3	A	C	13: 113,800,027 (GRCm39)	M1R	probably null	Het
Igkv10-95	T	C	6: 68,657,601 (GRCm39)	V19A	probably damaging	Het
Il1rap	A	G	16: 26,541,636 (GRCm39)	S626G	probably benign	Het
Ints9	A	G	14: 65,228,003 (GRCm39)	H103R	probably benign	Het
Itga4	T	A	2: 79,152,996 (GRCm39)	D894E	probably damaging	Het
Khsrp	T	C	17: 57,332,605 (GRCm39)	H225R	probably benign	Het
Lama1	T	C	17: 68,057,650 (GRCm39)	Y575H	probably damaging	Het
Lama1	A	T	17: 68,119,481 (GRCm39)	I2653F	probably damaging	Het
Map4k5	A	T	12: 69,892,497 (GRCm39)	L144Q	probably damaging	Het
Mcm5	A	G	8: 75,842,482 (GRCm39)	Y252C	probably damaging	Het
Mmgt2	A	G	11: 62,555,877 (GRCm39)	E75G	probably damaging	Het
Mroh2a	A	T	1: 88,182,687 (GRCm39)	N1205I	possibly damaging	Het
Nckap5	A	G	1: 126,150,443 (GRCm39)	V162A	probably benign	Het
Nr3c2	T	C	8: 77,636,378 (GRCm39)	I493T	probably damaging	Het
Or2ag16	A	C	7: 106,352,286 (GRCm39)	L103R	probably damaging	Het
Or2t46	A	T	11: 58,471,901 (GRCm39)	Y77F	probably damaging	Het
Papola	A	G	12: 105,765,917 (GRCm39)	T6A	possibly damaging	Het
Ppp1cc	G	A	5: 122,306,289 (GRCm39)	R36Q	probably benign	Het
Ptpro	G	T	6: 137,397,370 (GRCm39)	W81C	probably damaging	Het
Rasgrf2	A	G	13: 92,130,773 (GRCm39)	S162P	possibly damaging	Het
Rpusd2	T	C	2: 118,869,196 (GRCm39)	S540P	probably damaging	Het
Scn5a	T	A	9: 119,315,438 (GRCm39)	T1757S	probably damaging	Het
Setbp1	T	C	18: 78,900,206 (GRCm39)	I1154V	probably benign	Het
Sirt1	T	C	10: 63,171,438 (GRCm39)	I209V	probably null	Het
Slco6c1	T	A	1: 97,009,218 (GRCm39)	I406L	probably benign	Het
Smc2	A	G	4: 52,450,947 (GRCm39)	E255G	probably damaging	Het
Snap91	C	A	9: 86,659,102 (GRCm39)	G477V	probably damaging	Het
Speg	A	G	1: 75,404,548 (GRCm39)	Q2780R	probably benign	Het
Spmap2	T	C	10: 79,415,884 (GRCm39)	T236A	probably damaging	Het
Ssh2	A	G	11: 77,312,095 (GRCm39)	Y196C	probably damaging	Het
Ssxb9	A	T	X: 8,235,845 (GRCm39)	T18S	probably damaging	Het
Stxbp5l	T	C	16: 37,028,481 (GRCm39)	I527M	possibly damaging	Het
Tex10	T	C	4: 48,468,968 (GRCm39)	Y69C	probably damaging	Het
Thoc3	T	C	13: 54,616,361 (GRCm39)	D87G	probably benign	Het
Top2b	T	G	14: 16,409,189 (GRCm38)	I777M	probably damaging	Het
Trps1	T	C	15: 50,694,783 (GRCm39)	K213R	probably damaging	Het
Uggt1	A	T	1: 36,197,240 (GRCm39)	L1221Q	probably damaging	Het
Vmn2r3	T	A	3: 64,183,138 (GRCm39)	Y187F	probably damaging	Het
Washc2	G	A	6: 116,235,891 (GRCm39)	E1121K	probably damaging	Het
Zfx	T	C	X: 93,124,464 (GRCm39)	N360D	probably damaging	Het
Znhit3	A	G	11: 84,807,139 (GRCm39)	V10A	probably benign	Het

Other mutations in Or9s15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01382:Or9s15	APN	1	92,524,922 (GRCm39)	missense	possibly damaging	0.46
IGL02011:Or9s15	APN	1	92,524,621 (GRCm39)	missense	probably damaging	1.00
PIT4810001:Or9s15	UTSW	1	92,524,876 (GRCm39)	missense	probably benign	0.04
R2036:Or9s15	UTSW	1	92,524,328 (GRCm39)	missense	probably benign	0.19
R2044:Or9s15	UTSW	1	92,524,691 (GRCm39)	missense	probably benign	0.00
R4406:Or9s15	UTSW	1	92,525,036 (GRCm39)	missense	possibly damaging	0.90
R4568:Or9s15	UTSW	1	92,525,113 (GRCm39)	missense	probably benign	0.09
R4701:Or9s15	UTSW	1	92,525,160 (GRCm39)	missense	probably benign	0.00
R4801:Or9s15	UTSW	1	92,524,720 (GRCm39)	missense	probably benign	0.01
R4802:Or9s15	UTSW	1	92,524,720 (GRCm39)	missense	probably benign	0.01
R6564:Or9s15	UTSW	1	92,524,285 (GRCm39)	missense	probably benign
R7082:Or9s15	UTSW	1	92,524,140 (GRCm39)	start gained	probably benign
R7349:Or9s15	UTSW	1	92,524,904 (GRCm39)	missense	possibly damaging	0.95
R7589:Or9s15	UTSW	1	92,524,781 (GRCm39)	missense	probably benign	0.00
R8816:Or9s15	UTSW	1	92,524,768 (GRCm39)	missense	probably damaging	1.00
R9051:Or9s15	UTSW	1	92,524,978 (GRCm39)	missense	probably damaging	1.00
R9267:Or9s15	UTSW	1	92,524,996 (GRCm39)	missense	probably benign
R9267:Or9s15	UTSW	1	92,524,994 (GRCm39)	missense	probably damaging	1.00
Z1177:Or9s15	UTSW	1	92,524,988 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- CAGGAATGGAAGCCTCTCAG -3'
(R):5'- GTACCTCAGAGGACTGCAGATG -3'

Sequencing Primer
(F):5'- AATGGAAGCCTCTCAGCAGTGTC -3'
(R):5'- CTGCAGATGGCCATGAAACG -3'

Posted On

2015-05-14