Mutagenetix > Incidental Mutation

Incidental Mutation 'R4120:Zfp936'

315271

Institutional Source

Beutler Lab

Gene Symbol

Zfp936

Ensembl Gene

ENSMUSG00000064194

Gene Name

zinc finger protein 936

Synonyms

EG435970, Gm9272, I1C0022H11Rik

MMRRC Submission

040993-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.670) question?

Stock #

R4120 (G1)

Quality Score

123

Status

Validated

Chromosome

Chromosomal Location

42763653-42841533 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 42839630 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 366 (T366A)

Ref Sequence

ENSEMBL: ENSMUSP00000143800 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072829] [ENSMUST00000200973] [ENSMUST00000202535] [ENSMUST00000205912]

AlphaFold

Q3ULA8

Predicted Effect

probably benign
Transcript: ENSMUST00000072829
AA Change: T365A

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000072608
Gene: ENSMUSG00000064194
AA Change: T365A

Domain	Start	End	E-Value	Type
KRAB	3	65	5.32e-19	SMART
ZnF_C2H2	148	170	5.9e-3	SMART
ZnF_C2H2	176	198	5.9e-3	SMART
ZnF_C2H2	204	226	1.4e-4	SMART
ZnF_C2H2	232	254	1.2e-3	SMART
ZnF_C2H2	260	282	7.37e-4	SMART
ZnF_C2H2	288	310	2.27e-4	SMART
ZnF_C2H2	316	338	2.09e-3	SMART
ZnF_C2H2	344	366	1.45e-2	SMART
ZnF_C2H2	372	394	1.82e-3	SMART
ZnF_C2H2	400	422	2.53e-2	SMART
ZnF_C2H2	428	450	4.54e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000200973

SMART Domains

Protein: ENSMUSP00000144191
Gene: ENSMUSG00000064194

Domain	Start	End	E-Value	Type
KRAB	4	66	1.4e-21	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000202535
AA Change: T366A

PolyPhen 2 Score 0.102 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000143800
Gene: ENSMUSG00000064194
AA Change: T366A

Domain	Start	End	E-Value	Type
KRAB	4	66	2.3e-21	SMART
ZnF_C2H2	149	171	2.6e-5	SMART
ZnF_C2H2	177	199	2.5e-5	SMART
ZnF_C2H2	205	227	5.9e-7	SMART
ZnF_C2H2	233	255	5.1e-6	SMART
ZnF_C2H2	261	283	3.1e-6	SMART
ZnF_C2H2	289	311	9.4e-7	SMART
ZnF_C2H2	317	339	8.7e-6	SMART
ZnF_C2H2	345	367	6.3e-5	SMART
ZnF_C2H2	373	395	7.7e-6	SMART
ZnF_C2H2	401	423	1.1e-4	SMART
ZnF_C2H2	429	451	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000205912

Meta Mutation Damage Score

0.1273

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

93% (55/59)

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrb3	C	A	1: 25,133,388 (GRCm39)	E1233*	probably null	Het
Afap1l1	T	C	18: 61,872,243 (GRCm39)	D526G	probably damaging	Het
Ago4	A	G	4: 126,390,600 (GRCm39)	Y807H	probably damaging	Het
Ankfy1	G	A	11: 72,605,310 (GRCm39)		probably null	Het
Arhgap40	A	G	2: 158,374,250 (GRCm39)	M222V	probably benign	Het
Asnsd1	A	G	1: 53,387,154 (GRCm39)	S158P	probably damaging	Het
Cdh15	T	C	8: 123,590,162 (GRCm39)	V365A	probably damaging	Het
Clca3a2	T	C	3: 144,516,613 (GRCm39)	M328V	probably benign	Het
Col4a1	G	A	8: 11,256,263 (GRCm39)	P1535S	unknown	Het
Dennd2a	G	A	6: 39,442,030 (GRCm39)	R947C	probably damaging	Het
Dlg2	G	A	7: 91,614,846 (GRCm39)	V217I	probably damaging	Het
Dnaaf2	T	C	12: 69,244,812 (GRCm39)	D83G	possibly damaging	Het
Dppa5a	A	T	9: 78,275,137 (GRCm39)	M55K	possibly damaging	Het
Ect2l	A	G	10: 18,006,466 (GRCm39)	V779A	probably benign	Het
Ehhadh	A	G	16: 21,581,934 (GRCm39)	S353P	probably benign	Het
Etv3	A	G	3: 87,443,589 (GRCm39)	D391G	probably benign	Het
Fastkd1	T	A	2: 69,537,654 (GRCm39)	K309N	probably damaging	Het
Fip1l1	T	A	5: 74,748,852 (GRCm39)	Y375N	probably damaging	Het
Flvcr2	T	C	12: 85,832,903 (GRCm39)	S308P	probably benign	Het
Fyco1	T	C	9: 123,654,691 (GRCm39)	Y1065C	probably benign	Het
Gfra2	A	G	14: 71,203,715 (GRCm39)	D31G	probably damaging	Het
Gm17175	A	T	14: 51,810,534 (GRCm39)	I31N	probably damaging	Het
Gm6003	T	A	7: 32,864,976 (GRCm39)		noncoding transcript	Het
Gm9951	T	C	8: 34,522,993 (GRCm39)		noncoding transcript	Het
Hspa2	A	G	12: 76,452,008 (GRCm39)	E234G	probably damaging	Het
Ip6k2	G	A	9: 108,682,847 (GRCm39)	R319Q	probably benign	Het
Iqsec1	A	T	6: 90,639,584 (GRCm39)	Y1065*	probably null	Het
Ltk	T	A	2: 119,588,429 (GRCm39)		probably benign	Het
Map2	G	A	1: 66,455,063 (GRCm39)	A1318T	probably damaging	Het
Mas1	A	T	17: 13,061,233 (GRCm39)	N63K	probably damaging	Het
Myo15b	T	C	11: 115,764,318 (GRCm39)	S1311P	probably benign	Het
Nav3	A	G	10: 109,739,605 (GRCm39)		probably null	Het
Nlrp1c-ps	T	C	11: 71,133,359 (GRCm39)		noncoding transcript	Het
Oca2	A	T	7: 55,904,630 (GRCm39)	D32V	probably damaging	Het
Or51v8	T	C	7: 103,320,221 (GRCm39)	T6A	probably benign	Het
Or8b9	T	C	9: 37,766,705 (GRCm39)	L197S	possibly damaging	Het
Otub2	T	C	12: 103,370,489 (GRCm39)	V257A	probably damaging	Het
Pink1	G	A	4: 138,042,822 (GRCm39)	R461*	probably null	Het
Plxna2	T	C	1: 194,462,935 (GRCm39)	C901R	probably damaging	Het
Ptchd3	T	A	11: 121,721,572 (GRCm39)	N148K	probably damaging	Het
Ralgapa1	C	T	12: 55,687,429 (GRCm39)	R2019Q	probably damaging	Het
Ripor1	T	A	8: 106,345,489 (GRCm39)		probably benign	Het
Sec13	T	C	6: 113,711,637 (GRCm39)	N107S	probably damaging	Het
Slc12a2	A	G	18: 58,032,427 (GRCm39)	M376V	possibly damaging	Het
Sp140l2	C	T	1: 85,237,542 (GRCm39)	V81M	possibly damaging	Het
Sptbn5	T	A	2: 119,895,010 (GRCm39)	D798V	possibly damaging	Het
Stradb	T	A	1: 59,019,168 (GRCm39)	Y30N	possibly damaging	Het
Syne1	T	A	10: 5,359,798 (GRCm39)	Q328L	probably damaging	Het
Ttc23l	A	C	15: 10,540,006 (GRCm39)	V159G	probably damaging	Het
Vmn2r100	T	C	17: 19,752,215 (GRCm39)	S753P	probably damaging	Het
Vmn2r129	G	T	4: 156,686,778 (GRCm39)		noncoding transcript	Het
Vmn2r39	G	A	7: 9,026,673 (GRCm39)	H443Y	probably benign	Het
Zkscan3	T	C	13: 21,578,119 (GRCm39)	E256G	possibly damaging	Het

Other mutations in Zfp936

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02231:Zfp936	APN	7	42,836,909 (GRCm39)	splice site	probably null
IGL02245:Zfp936	APN	7	42,836,722 (GRCm39)	critical splice donor site	probably null
IGL02335:Zfp936	APN	7	42,836,691 (GRCm39)	missense	probably damaging	1.00
FR4340:Zfp936	UTSW	7	42,838,913 (GRCm39)	missense	possibly damaging	0.63
R0437:Zfp936	UTSW	7	42,838,734 (GRCm39)	missense	probably benign	0.00
R3899:Zfp936	UTSW	7	42,839,158 (GRCm39)	missense	possibly damaging	0.93
R4406:Zfp936	UTSW	7	42,839,748 (GRCm39)	missense	possibly damaging	0.82
R4959:Zfp936	UTSW	7	42,839,034 (GRCm39)	missense	probably damaging	1.00
R5023:Zfp936	UTSW	7	42,836,681 (GRCm39)	missense	probably damaging	1.00
R5163:Zfp936	UTSW	7	42,839,664 (GRCm39)	missense	probably damaging	1.00
R5182:Zfp936	UTSW	7	42,839,331 (GRCm39)	missense	probably damaging	1.00
R5292:Zfp936	UTSW	7	42,838,759 (GRCm39)	nonsense	probably null
R5668:Zfp936	UTSW	7	42,839,858 (GRCm39)	missense	possibly damaging	0.93
R6057:Zfp936	UTSW	7	42,839,787 (GRCm39)	missense	probably benign	0.00
R6901:Zfp936	UTSW	7	42,839,467 (GRCm39)	missense	probably damaging	1.00
R7139:Zfp936	UTSW	7	42,839,715 (GRCm39)	missense	possibly damaging	0.54
R7258:Zfp936	UTSW	7	42,839,803 (GRCm39)	missense	probably damaging	1.00
R7440:Zfp936	UTSW	7	42,836,685 (GRCm39)	missense	probably damaging	1.00
R7537:Zfp936	UTSW	7	42,839,239 (GRCm39)	nonsense	probably null
R7561:Zfp936	UTSW	7	42,839,339 (GRCm39)	missense	probably damaging	0.98
R7662:Zfp936	UTSW	7	42,839,336 (GRCm39)	nonsense	probably null
R7775:Zfp936	UTSW	7	42,839,720 (GRCm39)	missense	possibly damaging	0.70
R7778:Zfp936	UTSW	7	42,839,720 (GRCm39)	missense	possibly damaging	0.70
R8016:Zfp936	UTSW	7	42,838,848 (GRCm39)	missense	possibly damaging	0.61
R8121:Zfp936	UTSW	7	42,839,547 (GRCm39)	missense	possibly damaging	0.55
R9012:Zfp936	UTSW	7	42,839,416 (GRCm39)	nonsense	probably null
R9058:Zfp936	UTSW	7	42,839,196 (GRCm39)	missense	probably benign	0.32
R9188:Zfp936	UTSW	7	42,839,768 (GRCm39)	missense	probably benign	0.00
R9236:Zfp936	UTSW	7	42,836,922 (GRCm39)	missense	probably benign	0.00
R9596:Zfp936	UTSW	7	42,839,834 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCCTACCCACAGCAGAATTATCT -3'
(R):5'- TGTGTAAAAGCCTTACCACATTG -3'

Sequencing Primer
(F):5'- GTGATAAAGCCTTTTCACACCAGAG -3'
(R):5'- AGCCTTACCACATTGATCACATTTG -3'

Posted On

2015-05-14