|Institutional Source||Beutler Lab|
|Gene Name||a disintegrin and metallopeptidase domain 25 (testase 2)|
|Is this an essential gene?||Probably non essential (E-score: 0.068)|
|Stock #||R4061 (G1)|
|Chromosomal Location||40752208-40756176 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 40753782 bp|
|Amino Acid Change||Isoleucine to Methionine at position 28 (I28M)|
|Ref Sequence||ENSEMBL: ENSMUSP00000094420 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000096663]|
|Predicted Effect||possibly damaging
AA Change: I28M
PolyPhen 2 Score 0.665 (Sensitivity: 0.86; Specificity: 0.91)
AA Change: I28M
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.1795|
|Coding Region Coverage||
|Validation Efficiency||94% (51/54)|
|MGI Phenotype||FUNCTION: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is expressed in a regulated fashion during spermatogenesis. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional metalloprotease enzyme. This gene is located adjacent to other ADAM genes on chromosome 8. [provided by RefSeq, May 2016]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Adam25||
(F):5'- TGCCTTTGAACAAACCTCAGC -3'
(R):5'- TGTGAATAAAATGTTTCTGGCCCC -3'
(F):5'- TGGCCTGCCCATACTGAAATACTC -3'
(R):5'- AATGTTTCTGGCCCCCAAAATG -3'