Incidental Mutation 'R4076:Or5b3'
| ID |
316585 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Or5b3
|
| Ensembl Gene |
ENSMUSG00000063777 |
| Gene Name |
olfactory receptor family 5 subfamily B member 3 |
| Synonyms |
MOR202-11, Olfr1469, GA_x6K02T2RE5P-3743369-3744289 |
| MMRRC Submission |
040975-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.101)
|
| Stock # |
R4076 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
19 |
| Chromosomal Location |
13407849-13414540 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
G to A
at 13410935 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Histidine
at position 122
(R122H)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000150006
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000077538]
[ENSMUST00000216910]
|
| AlphaFold |
Q8VFW5 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000077538
AA Change: R122H
PolyPhen 2
Score 0.488 (Sensitivity: 0.88; Specificity: 0.90)
|
| SMART Domains |
Protein: ENSMUSP00000076741
Gene: ENSMUSG00000063777
AA Change: R122H
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_4
|
32 |
308 |
2e-47 |
PFAM |
|
Pfam:7tm_1
|
42 |
290 |
2.3e-19 |
PFAM |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000216910
AA Change: R122H
PolyPhen 2
Score 0.488 (Sensitivity: 0.88; Specificity: 0.90)
|
| Meta Mutation Damage Score |
0.2696 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.7%
- 10x: 97.5%
- 20x: 95.9%
|
| Validation Efficiency |
|
| MGI Phenotype |
FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 38 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Acsm4 |
T |
A |
7: 119,698,758 (GRCm38) |
L206H |
probably benign |
Het |
| Adam34l |
A |
T |
8: 43,626,350 (GRCm38) |
F279Y |
probably damaging |
Het |
| Anxa5 |
T |
C |
3: 36,450,380 (GRCm38) |
I277V |
probably benign |
Het |
| Atg12 |
A |
G |
18: 46,737,424 (GRCm38) |
F92L |
probably benign |
Het |
| Cd48 |
T |
A |
1: 171,695,883 (GRCm38) |
V98D |
probably damaging |
Het |
| Dnah11 |
A |
G |
12: 118,045,678 (GRCm38) |
M2083T |
probably benign |
Het |
| Dnah9 |
T |
C |
11: 66,084,904 (GRCm38) |
T1440A |
probably benign |
Het |
| Dnali1 |
T |
C |
4: 125,059,470 (GRCm38) |
D188G |
probably damaging |
Het |
| Dst |
A |
G |
1: 34,192,269 (GRCm38) |
E2656G |
probably benign |
Het |
| Eps15l1 |
A |
G |
8: 72,380,284 (GRCm38) |
I482T |
probably damaging |
Het |
| Ercc4 |
G |
A |
16: 13,130,685 (GRCm38) |
V499I |
probably damaging |
Het |
| Eva1c |
T |
A |
16: 90,904,131 (GRCm38) |
F331Y |
probably damaging |
Het |
| Fcho1 |
T |
C |
8: 71,710,369 (GRCm38) |
H672R |
probably damaging |
Het |
| Fras1 |
G |
C |
5: 96,743,158 (GRCm38) |
D2849H |
probably damaging |
Het |
| Hdc |
C |
T |
2: 126,616,261 (GRCm38) |
R47Q |
possibly damaging |
Het |
| Ighv1-30 |
C |
T |
12: 114,817,401 (GRCm38) |
|
noncoding transcript |
Het |
| Krtap31-1 |
T |
C |
11: 99,908,232 (GRCm38) |
I87T |
possibly damaging |
Het |
| Lamp3 |
A |
G |
16: 19,700,716 (GRCm38) |
L239P |
possibly damaging |
Het |
| Ltb4r2 |
A |
T |
14: 55,762,941 (GRCm38) |
R340W |
probably benign |
Het |
| Map10 |
T |
C |
8: 125,671,845 (GRCm38) |
V659A |
probably benign |
Het |
| Nars2 |
T |
A |
7: 96,958,094 (GRCm38) |
S91T |
probably damaging |
Het |
| Nlrp4c |
A |
G |
7: 6,072,710 (GRCm38) |
K667E |
probably benign |
Het |
| Obox3 |
G |
T |
7: 15,625,799 (GRCm38) |
T315N |
possibly damaging |
Het |
| Or52e18 |
A |
T |
7: 104,960,716 (GRCm38) |
N5K |
probably damaging |
Het |
| Osgin1 |
A |
C |
8: 119,445,033 (GRCm38) |
S189R |
possibly damaging |
Het |
| Pidd1 |
A |
G |
7: 141,440,826 (GRCm38) |
F453L |
probably damaging |
Het |
| Pik3c2g |
A |
T |
6: 139,852,863 (GRCm38) |
N373I |
probably damaging |
Het |
| Pou2f2 |
T |
C |
7: 25,097,288 (GRCm38) |
T270A |
probably damaging |
Het |
| Rad51b |
T |
G |
12: 79,314,882 (GRCm38) |
S122R |
probably damaging |
Het |
| Rev1 |
T |
G |
1: 38,054,238 (GRCm38) |
K1075T |
possibly damaging |
Het |
| Rrbp1 |
T |
G |
2: 143,963,110 (GRCm38) |
Q1045P |
probably benign |
Het |
| Scg2 |
A |
C |
1: 79,436,857 (GRCm38) |
F50V |
probably damaging |
Het |
| Slco1a5 |
A |
T |
6: 142,268,224 (GRCm38) |
I57K |
possibly damaging |
Het |
| Tfb1m |
T |
A |
17: 3,521,670 (GRCm38) |
R257W |
probably damaging |
Het |
| Tm2d3 |
T |
A |
7: 65,697,750 (GRCm38) |
L49* |
probably null |
Het |
| Usp31 |
A |
G |
7: 121,667,782 (GRCm38) |
|
probably null |
Het |
| Zbtb11 |
C |
T |
16: 55,998,064 (GRCm38) |
T617I |
possibly damaging |
Het |
| Zfp608 |
A |
T |
18: 54,898,108 (GRCm38) |
V920E |
probably damaging |
Het |
|
| Other mutations in Or5b3 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01064:Or5b3
|
APN |
19 |
13,411,226 (GRCm38) |
missense |
probably benign |
0.01 |
|
IGL01520:Or5b3
|
APN |
19 |
13,410,750 (GRCm38) |
missense |
probably damaging |
0.97 |
|
IGL01671:Or5b3
|
APN |
19 |
13,410,891 (GRCm38) |
missense |
probably benign |
0.29 |
|
IGL02247:Or5b3
|
APN |
19 |
13,411,467 (GRCm38) |
missense |
probably benign |
0.01 |
|
IGL02297:Or5b3
|
APN |
19 |
13,411,475 (GRCm38) |
missense |
probably benign |
0.00 |
|
IGL02417:Or5b3
|
APN |
19 |
13,410,895 (GRCm38) |
missense |
possibly damaging |
0.52 |
|
IGL02442:Or5b3
|
APN |
19 |
13,410,987 (GRCm38) |
missense |
probably benign |
0.00 |
|
IGL02989:Or5b3
|
APN |
19 |
13,411,486 (GRCm38) |
missense |
probably benign |
|
|
IGL03269:Or5b3
|
APN |
19 |
13,411,428 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL02988:Or5b3
|
UTSW |
19 |
13,411,462 (GRCm38) |
missense |
possibly damaging |
0.75 |
|
R0707:Or5b3
|
UTSW |
19 |
13,411,420 (GRCm38) |
missense |
probably benign |
0.22 |
|
R1055:Or5b3
|
UTSW |
19 |
13,411,390 (GRCm38) |
missense |
probably benign |
0.10 |
|
R1102:Or5b3
|
UTSW |
19 |
13,411,090 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1946:Or5b3
|
UTSW |
19 |
13,410,779 (GRCm38) |
missense |
possibly damaging |
0.64 |
|
R2111:Or5b3
|
UTSW |
19 |
13,410,943 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R4072:Or5b3
|
UTSW |
19 |
13,410,935 (GRCm38) |
missense |
possibly damaging |
0.49 |
|
R4073:Or5b3
|
UTSW |
19 |
13,410,935 (GRCm38) |
missense |
possibly damaging |
0.49 |
|
R4726:Or5b3
|
UTSW |
19 |
13,411,105 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4939:Or5b3
|
UTSW |
19 |
13,410,855 (GRCm38) |
missense |
probably benign |
0.10 |
|
R5914:Or5b3
|
UTSW |
19 |
13,410,962 (GRCm38) |
missense |
probably benign |
0.31 |
|
R6003:Or5b3
|
UTSW |
19 |
13,411,039 (GRCm38) |
missense |
probably benign |
0.34 |
|
R6743:Or5b3
|
UTSW |
19 |
13,410,593 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6825:Or5b3
|
UTSW |
19 |
13,411,150 (GRCm38) |
missense |
probably benign |
0.01 |
|
R6826:Or5b3
|
UTSW |
19 |
13,411,088 (GRCm38) |
missense |
probably benign |
0.05 |
|
R6970:Or5b3
|
UTSW |
19 |
13,411,428 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R7558:Or5b3
|
UTSW |
19 |
13,410,991 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7596:Or5b3
|
UTSW |
19 |
13,411,147 (GRCm38) |
missense |
probably benign |
0.01 |
|
R7923:Or5b3
|
UTSW |
19 |
13,410,818 (GRCm38) |
missense |
probably benign |
0.17 |
|
R8014:Or5b3
|
UTSW |
19 |
13,410,811 (GRCm38) |
missense |
not run |
|
|
R8506:Or5b3
|
UTSW |
19 |
13,411,240 (GRCm38) |
missense |
possibly damaging |
0.49 |
|
R8746:Or5b3
|
UTSW |
19 |
13,410,728 (GRCm38) |
missense |
probably benign |
0.44 |
|
R8803:Or5b3
|
UTSW |
19 |
13,410,673 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R9112:Or5b3
|
UTSW |
19 |
13,411,111 (GRCm38) |
missense |
probably benign |
0.05 |
|
R9721:Or5b3
|
UTSW |
19 |
13,410,970 (GRCm38) |
missense |
probably benign |
0.17 |
|
Z1177:Or5b3
|
UTSW |
19 |
13,410,719 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
| Predicted Primers |
PCR Primer
(F):5'- TTCTGCTGATTGTCCTGGAC -3'
(R):5'- TCACAGAAGAAGTGATGCACTAC -3'
Sequencing Primer
(F):5'- GCTGATTGTCCTGGACTCTCG -3'
(R):5'- GTGATGCACTACATTAGACCTACAG -3'
|
| Posted On |
2015-05-15 |
Incidental Mutation