Mutagenetix > Incidental Mutations

Incidental Mutation 'R4080:Zfp667'

316813

Institutional Source

Beutler Lab

Gene Symbol

Zfp667

Ensembl Gene

ENSMUSG00000054893

Gene Name

zinc finger protein 667

Synonyms

A830025F02Rik

MMRRC Submission

040856-MU

Accession Numbers

Genbank: NM_001024928

Is this an essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R4080 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

6286579-6307883 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 6305106 bp

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Glycine at position 258 (C258G)

Ref Sequence

ENSEMBL: ENSMUSP00000128658 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086327] [ENSMUST00000108562] [ENSMUST00000153840] [ENSMUST00000170776]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000086327
AA Change: C258G

PolyPhen 2 Score 0.711 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000083507
Gene: ENSMUSG00000054893
AA Change: C258G

Domain	Start	End	E-Value	Type
KRAB	14	74	4.77e-30	SMART
ZnF_C2H2	144	166	5.42e-2	SMART
ZnF_C2H2	172	194	3.11e-2	SMART
ZnF_C2H2	200	222	1.67e-2	SMART
ZnF_C2H2	253	275	2.57e-3	SMART
ZnF_C2H2	329	351	2.4e-3	SMART
ZnF_C2H2	357	379	3.16e-3	SMART
ZnF_C2H2	385	407	8.94e-3	SMART
ZnF_C2H2	414	436	5.06e-2	SMART
ZnF_C2H2	442	464	2.4e-3	SMART
ZnF_C2H2	470	492	5.29e-5	SMART
ZnF_C2H2	498	520	7.37e-4	SMART
ZnF_C2H2	526	548	1.38e-3	SMART
ZnF_C2H2	554	576	1.13e-4	SMART
ZnF_C2H2	582	604	1.38e-3	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000108562
AA Change: C258G

PolyPhen 2 Score 0.711 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000104202
Gene: ENSMUSG00000054893
AA Change: C258G

Domain	Start	End	E-Value	Type
KRAB	14	74	4.77e-30	SMART
ZnF_C2H2	144	166	5.42e-2	SMART
ZnF_C2H2	172	194	3.11e-2	SMART
ZnF_C2H2	200	222	1.67e-2	SMART
ZnF_C2H2	253	275	2.57e-3	SMART
ZnF_C2H2	329	351	2.4e-3	SMART
ZnF_C2H2	357	379	3.16e-3	SMART
ZnF_C2H2	385	407	8.94e-3	SMART
ZnF_C2H2	414	436	5.06e-2	SMART
ZnF_C2H2	442	464	2.4e-3	SMART
ZnF_C2H2	470	492	5.29e-5	SMART
ZnF_C2H2	498	520	7.37e-4	SMART
ZnF_C2H2	526	548	1.38e-3	SMART
ZnF_C2H2	554	576	1.13e-4	SMART
ZnF_C2H2	582	604	1.38e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000153840

Predicted Effect

possibly damaging
Transcript: ENSMUST00000170776
AA Change: C258G

PolyPhen 2 Score 0.711 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000128658
Gene: ENSMUSG00000054893
AA Change: C258G

Domain	Start	End	E-Value	Type
KRAB	14	74	4.77e-30	SMART
ZnF_C2H2	144	166	5.42e-2	SMART
ZnF_C2H2	172	194	3.11e-2	SMART
ZnF_C2H2	200	222	1.67e-2	SMART
ZnF_C2H2	253	275	2.57e-3	SMART
ZnF_C2H2	329	351	2.4e-3	SMART
ZnF_C2H2	357	379	3.16e-3	SMART
ZnF_C2H2	385	407	8.94e-3	SMART
ZnF_C2H2	414	436	5.06e-2	SMART
ZnF_C2H2	442	464	2.4e-3	SMART
ZnF_C2H2	470	492	5.29e-5	SMART
ZnF_C2H2	498	520	7.37e-4	SMART
ZnF_C2H2	526	548	1.38e-3	SMART
ZnF_C2H2	554	576	1.13e-4	SMART
ZnF_C2H2	582	604	1.38e-3	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

Allele List at MGI

All alleles(4) : Targeted, other(2) Gene trapped(2)

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110008L16Rik	G	T	12: 55,304,613	V236F	possibly damaging	Het
Aass	A	T	6: 23,109,498	D324E	possibly damaging	Het
Adam7	T	C	14: 68,520,539	T245A	probably benign	Het
Adgrf3	G	A	5: 30,197,369	Q554*	probably null	Het
Aim2	T	C	1: 173,459,851		probably null	Het
Arhgef1	G	T	7: 24,925,846	D850Y	probably damaging	Het
Aspm	C	A	1: 139,470,755	Q1024K	probably damaging	Het
C7	T	C	15: 4,990,464	S734G	probably benign	Het
Ccdc158	A	T	5: 92,623,396	S987T	probably benign	Het
Chrna2	G	T	14: 66,143,417	G45V	probably benign	Het
Chrna2	C	A	14: 66,143,424	Y47*	probably null	Het
Clec2g	C	A	6: 128,981,324	Q117K	probably damaging	Het
Cntnap5a	A	G	1: 116,101,574	S253G	probably benign	Het
Cttn	T	A	7: 144,457,724	D116V	probably damaging	Het
Cyp2c40	A	G	19: 39,802,529	V286A	probably benign	Het
Dcbld2	T	A	16: 58,465,373	S632T	probably damaging	Het
Dscam	A	T	16: 96,683,772	N1118K	probably benign	Het
Eif2a	C	T	3: 58,539,629	T92M	possibly damaging	Het
Frmpd1	T	A	4: 45,284,382	C1068S	probably benign	Het
Fstl1	G	A	16: 37,822,603	V110I	probably benign	Het
Gpat2	T	C	2: 127,433,622	I465T	probably damaging	Het
Gpr137	C	T	19: 6,940,423		probably benign	Het
Hgsnat	A	G	8: 25,946,343	I561T	probably benign	Het
Ift74	T	A	4: 94,652,912		probably null	Het
Ilf3	A	G	9: 21,403,134		probably null	Het
Kcnh8	GAGACCAACGAGCAGCTGATGCTTCAGA	GAGA	17: 52,725,906		probably benign	Het
Klk14	G	A	7: 43,692,077	C51Y	probably damaging	Het
Lrrc41	C	A	4: 116,080,546		probably null	Het
Myh11	C	A	16: 14,224,059	R700L	possibly damaging	Het
Myo16	A	G	8: 10,562,240	D1295G	probably damaging	Het
Myo5b	A	G	18: 74,740,488	M1488V	probably benign	Het
Naip6	A	T	13: 100,299,307	Y903N	probably damaging	Het
Nek6	A	G	2: 38,550,637	H19R	probably damaging	Het
Nktr	A	C	9: 121,741,126	T127P	probably damaging	Het
Noc4l	A	T	5: 110,649,872	D335E	probably benign	Het
Nsd1	A	G	13: 55,301,809	D1993G	probably damaging	Het
Olfr1377	G	A	11: 50,984,856	D52N	probably damaging	Het
Olfr190	A	T	16: 59,074,256	F275I	probably damaging	Het
Pabpc2	A	T	18: 39,775,530	Q616L	possibly damaging	Het
Pcdhga4	A	T	18: 37,685,779	D127V	probably damaging	Het
Phrf1	T	C	7: 141,259,720		probably benign	Het
Phtf2	T	A	5: 20,813,296	I16F	probably damaging	Het
Plekhg3	G	T	12: 76,577,981	R1200L	probably benign	Het
Plod3	G	C	5: 136,988,146	A50P	probably benign	Het
Prss12	A	G	3: 123,485,485	N404D	probably benign	Het
Ptch2	C	G	4: 117,111,206	A926G	probably damaging	Het
Ptpa	T	C	2: 30,443,305	F6L	probably damaging	Het
Reck	T	C	4: 43,942,293	I853T	possibly damaging	Het
Reep6	G	A	10: 80,330,162		probably benign	Het
Rex2	T	A	4: 147,058,697	S547R	probably benign	Het
Rgs22	C	T	15: 36,107,076	E55K	probably damaging	Het
Rtl6	T	C	15: 84,557,001	T65A	possibly damaging	Het
Scarb1	G	T	5: 125,277,795	P491Q	probably damaging	Het
Scfd1	A	G	12: 51,431,519	S505G	probably benign	Het
Scube1	T	G	15: 83,608,747	Q904P	probably damaging	Het
Sis	T	C	3: 72,921,184	Y1186C	probably damaging	Het
Spta1	A	G	1: 174,214,066	D1334G	probably benign	Het
Spty2d1	T	C	7: 46,998,581	E200G	probably damaging	Het
Stard13	C	A	5: 151,092,829		probably null	Het
Sybu	T	C	15: 44,718,943	K95R	probably damaging	Het
Trappc9	T	A	15: 72,941,947	D488V	probably damaging	Het
Txk	G	A	5: 72,700,663	P381S	probably damaging	Het
Ubr2	A	T	17: 46,988,722	M198K	probably benign	Het
Unc5a	A	T	13: 55,004,481	T786S	possibly damaging	Het
Unc93b1	G	A	19: 3,941,959	R231Q	probably damaging	Het
Wfdc1	T	A	8: 119,683,793		probably null	Het
Zfr	G	A	15: 12,162,233	R823H	probably benign	Het

Other mutations in Zfp667

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00821:Zfp667	APN	7	6305397	missense	possibly damaging	0.53
IGL01325:Zfp667	APN	7	6290546	missense	probably damaging	1.00
IGL01386:Zfp667	APN	7	6304870	missense	probably benign	0.00
IGL01960:Zfp667	APN	7	6305337	missense	probably benign	0.00
IGL03394:Zfp667	APN	7	6289439	critical splice donor site	probably null
B5639:Zfp667	UTSW	7	6290545	missense	probably damaging	1.00
R0458:Zfp667	UTSW	7	6304845	missense	probably benign	0.40
R0845:Zfp667	UTSW	7	6306092	missense	possibly damaging	0.85
R1768:Zfp667	UTSW	7	6305067	missense	possibly damaging	0.53
R1953:Zfp667	UTSW	7	6305088	missense	probably benign	0.04
R2023:Zfp667	UTSW	7	6305417	missense	possibly damaging	0.85
R3159:Zfp667	UTSW	7	6306000	missense	probably damaging	1.00
R4476:Zfp667	UTSW	7	6304599	missense	possibly damaging	0.53
R4584:Zfp667	UTSW	7	6290625	missense	possibly damaging	0.84
R4783:Zfp667	UTSW	7	6305685	missense	possibly damaging	0.83
R5037:Zfp667	UTSW	7	6305950	missense	possibly damaging	0.71
R5300:Zfp667	UTSW	7	6304636	missense	probably benign
R5311:Zfp667	UTSW	7	6305716	missense	probably benign	0.10
R5312:Zfp667	UTSW	7	6305467	missense	probably benign
R5340:Zfp667	UTSW	7	6305253	missense	possibly damaging	0.53
R6262:Zfp667	UTSW	7	6304974	missense	probably benign	0.03
R7386:Zfp667	UTSW	7	6305950	missense	possibly damaging	0.86
R8383:Zfp667	UTSW	7	6305371	missense	probably damaging	0.98
Z1177:Zfp667	UTSW	7	6304857	missense	possibly damaging	0.91

Predicted Primers

PCR Primer
(F):5'- CCTCATTCTTCATCAGCGGG -3'
(R):5'- GCCTGGCACTCTGAATTAAGG -3'

Sequencing Primer
(F):5'- CATCAGCGGGTTCACAATTG -3'
(R):5'- GGCACTCTGAATTAAGGCCTTC -3'

Posted On

2015-05-15