Mutagenetix > Incidental Mutation

Incidental Mutation 'R4084:Zfp1005'

317006

Institutional Source

Beutler Lab

Gene Symbol

Zfp1005

Ensembl Gene

ENSMUSG00000079008

Gene Name

zinc finger protein 1005

Synonyms

Gm10749, EG640962, Gm14124

MMRRC Submission

040857-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R4084 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

150099437-150112220 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 150108122 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 27 (N27K)

Ref Sequence

ENSEMBL: ENSMUSP00000105548 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109922]

AlphaFold

A2AU83

Predicted Effect

possibly damaging
Transcript: ENSMUST00000109922
AA Change: N27K

PolyPhen 2 Score 0.847 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000105548
Gene: ENSMUSG00000079008
AA Change: N27K

Domain	Start	End	E-Value	Type
KRAB	4	66	9.26e-19	SMART
ZnF_C2H2	103	125	7.49e-5	SMART
ZnF_C2H2	131	151	9.46e0	SMART
ZnF_C2H2	159	181	5.9e-3	SMART
ZnF_C2H2	187	209	6.67e-2	SMART
ZnF_C2H2	215	237	4.87e-4	SMART
ZnF_C2H2	243	265	2.82e0	SMART
ZnF_C2H2	271	293	2.2e2	SMART
ZnF_C2H2	299	321	1.4e-4	SMART
ZnF_C2H2	327	349	1.6e-4	SMART
ZnF_C2H2	355	377	1.18e-2	SMART
ZnF_C2H2	383	405	1.38e-3	SMART
ZnF_C2H2	411	433	9.56e1	SMART
ZnF_C2H2	439	461	6.99e-5	SMART
ZnF_C2H2	467	489	2.99e-4	SMART
ZnF_C2H2	495	517	7.78e-3	SMART
ZnF_C2H2	523	545	1.04e-3	SMART
ZnF_C2H2	551	573	1.6e-4	SMART
ZnF_C2H2	579	601	1.18e-2	SMART
ZnF_C2H2	607	629	4.54e-4	SMART
ZnF_C2H2	635	657	4.24e-4	SMART
ZnF_C2H2	663	685	1.2e-3	SMART
ZnF_C2H2	691	713	8.47e-4	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Amotl2	A	T	9: 102,601,884 (GRCm39)		probably null	Het
Arhgdig	T	C	17: 26,418,799 (GRCm39)	D114G	possibly damaging	Het
Btnl1	C	T	17: 34,600,133 (GRCm39)	T212I	possibly damaging	Het
Camkk1	C	T	11: 72,928,691 (GRCm39)	T410I	probably damaging	Het
Capn13	C	T	17: 73,644,444 (GRCm39)	G362R	probably benign	Het
Catsperd	A	G	17: 56,961,453 (GRCm39)	T392A	probably benign	Het
Ccdc180	A	G	4: 45,950,632 (GRCm39)	I1626V	probably benign	Het
Cdon	C	A	9: 35,389,427 (GRCm39)	T844K	probably damaging	Het
Col28a1	G	A	6: 8,013,131 (GRCm39)	Q974*	probably null	Het
Col28a1	C	G	6: 8,013,132 (GRCm39)	K973N	possibly damaging	Het
Dnhd1	T	C	7: 105,358,795 (GRCm39)	L3428P	probably damaging	Het
Ecm1	A	T	3: 95,641,676 (GRCm39)	N519K	probably damaging	Het
Fbxw11	T	C	11: 32,689,248 (GRCm39)	V457A	probably damaging	Het
Flna	C	T	X: 73,280,531 (GRCm39)	V1009M	possibly damaging	Het
Fmnl2	A	T	2: 52,997,507 (GRCm39)	K486I	possibly damaging	Het
Gja1	A	C	10: 56,264,607 (GRCm39)	Q322P	possibly damaging	Het
Gtpbp3	A	G	8: 71,943,156 (GRCm39)	Q189R	probably benign	Het
H2-Eb1	T	C	17: 34,533,417 (GRCm39)	V213A	probably damaging	Het
Herc4	G	T	10: 63,119,016 (GRCm39)	G322V	probably damaging	Het
Hgf	G	T	5: 16,820,856 (GRCm39)	G668*	probably null	Het
Htra1	T	C	7: 130,538,074 (GRCm39)	S25P	probably benign	Het
Ifi44	A	G	3: 151,451,126 (GRCm39)		probably null	Het
Klhl24	T	A	16: 19,933,312 (GRCm39)	S308T	probably damaging	Het
Lamb2	A	G	9: 108,365,217 (GRCm39)	N1291S	probably benign	Het
Lgals9	A	T	11: 78,860,589 (GRCm39)	F162Y	possibly damaging	Het
Lig3	T	A	11: 82,686,250 (GRCm39)	I634N	probably damaging	Het
Lipn	T	C	19: 34,056,340 (GRCm39)	F229L	probably benign	Het
Lmtk3	T	A	7: 45,442,716 (GRCm39)	S466R	probably damaging	Het
Lonrf2	G	A	1: 38,860,232 (GRCm39)	T22I	probably benign	Het
Macf1	T	C	4: 123,343,865 (GRCm39)	H2119R	probably damaging	Het
Muc6	C	T	7: 141,234,920 (GRCm39)	C634Y	probably damaging	Het
Nap1l1	G	A	10: 111,325,938 (GRCm39)	V86I	possibly damaging	Het
Noxred1	A	G	12: 87,280,258 (GRCm39)	Y25H	possibly damaging	Het
Nphp4	T	C	4: 152,573,248 (GRCm39)	L62P	probably damaging	Het
Or11h6	T	A	14: 50,880,305 (GRCm39)	I189N	probably damaging	Het
Or2b2	G	A	13: 21,887,238 (GRCm39)	W22*	probably null	Het
Or2b2	C	A	13: 21,887,239 (GRCm39)	L23M	probably damaging	Het
Or52ab4	T	G	7: 102,987,527 (GRCm39)	F89V	probably damaging	Het
Pcdh10	A	C	3: 45,347,142 (GRCm39)	D979A	probably damaging	Het
Pla2g4f	C	G	2: 120,142,806 (GRCm39)	Q101H	probably benign	Het
Ppp1r15b	T	C	1: 133,060,805 (GRCm39)	F441L	probably damaging	Het
Prkaca	C	A	8: 84,721,939 (GRCm39)	P309T	probably damaging	Het
Ptch1	T	G	13: 63,672,773 (GRCm39)	E944A	probably benign	Het
Ripor3	A	G	2: 167,826,386 (GRCm39)	Y720H	possibly damaging	Het
Rpgrip1	A	G	14: 52,386,808 (GRCm39)	E751G	possibly damaging	Het
Rsf1	GCGGCGGCGGCGGCGGC	GCGGCGGCGGCGGCGGCGGCGGCGGC	7: 97,229,126 (GRCm39)		probably benign	Het
Ryr3	A	G	2: 112,731,253 (GRCm39)	S686P	probably damaging	Het
Seh1l	T	C	18: 67,921,860 (GRCm39)	V240A	possibly damaging	Het
Slc10a2	T	G	8: 5,139,126 (GRCm39)	I273L	possibly damaging	Het
Slc23a2	A	T	2: 131,933,137 (GRCm39)	L107*	probably null	Het
Slc44a4	T	C	17: 35,136,323 (GRCm39)	L38P	probably damaging	Het
Slc6a18	C	T	13: 73,815,148 (GRCm39)	V387I	probably benign	Het
Slu7	G	A	11: 43,334,218 (GRCm39)	A415T	probably benign	Het
Tlr5	A	T	1: 182,802,413 (GRCm39)	R572S	possibly damaging	Het
Tmem45a2	C	T	16: 56,891,387 (GRCm39)	G3D	probably benign	Het
Trim24	A	G	6: 37,892,192 (GRCm39)	T242A	probably damaging	Het
Triobp	T	A	15: 78,857,871 (GRCm39)	N1157K	probably benign	Het
Ugt1a6a	A	T	1: 88,066,899 (GRCm39)	D235V	probably benign	Het
Vmn2r37	C	T	7: 9,218,984 (GRCm39)	V467I	probably benign	Het
Vmn2r7	C	T	3: 64,600,414 (GRCm39)	E495K	probably benign	Het
Vstm2a	A	G	11: 16,213,098 (GRCm39)	E161G	probably damaging	Het
Ypel3	T	C	7: 126,377,537 (GRCm39)	V74A	possibly damaging	Het
Zfp27	C	T	7: 29,594,792 (GRCm39)	R391H	possibly damaging	Het

Other mutations in Zfp1005

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01550:Zfp1005	APN	2	150,108,363 (GRCm39)	splice site	probably benign
R0220:Zfp1005	UTSW	2	150,110,595 (GRCm39)	missense	unknown
R0396:Zfp1005	UTSW	2	150,109,973 (GRCm39)	missense	probably damaging	1.00
R0402:Zfp1005	UTSW	2	150,111,136 (GRCm39)	missense	possibly damaging	0.93
R0446:Zfp1005	UTSW	2	150,109,993 (GRCm39)	missense	possibly damaging	0.71
R0462:Zfp1005	UTSW	2	150,111,122 (GRCm39)	missense	possibly damaging	0.80
R0507:Zfp1005	UTSW	2	150,110,044 (GRCm39)	missense	possibly damaging	0.69
R0605:Zfp1005	UTSW	2	150,110,523 (GRCm39)	missense	unknown
R0838:Zfp1005	UTSW	2	150,111,220 (GRCm39)	missense	possibly damaging	0.74
R1327:Zfp1005	UTSW	2	150,108,070 (GRCm39)	missense	possibly damaging	0.71
R1405:Zfp1005	UTSW	2	150,109,620 (GRCm39)	nonsense	probably null
R1405:Zfp1005	UTSW	2	150,109,620 (GRCm39)	nonsense	probably null
R2114:Zfp1005	UTSW	2	150,109,819 (GRCm39)	missense	unknown
R2140:Zfp1005	UTSW	2	150,111,281 (GRCm39)	missense	probably benign	0.33
R3683:Zfp1005	UTSW	2	150,109,976 (GRCm39)	missense	probably benign	0.41
R3917:Zfp1005	UTSW	2	150,108,039 (GRCm39)	splice site	probably benign
R4499:Zfp1005	UTSW	2	150,111,362 (GRCm39)	missense	possibly damaging	0.93
R4683:Zfp1005	UTSW	2	150,108,390 (GRCm39)	missense	possibly damaging	0.53
R4762:Zfp1005	UTSW	2	150,109,549 (GRCm39)	missense	possibly damaging	0.53
R4937:Zfp1005	UTSW	2	150,110,680 (GRCm39)	missense	unknown
R5678:Zfp1005	UTSW	2	150,110,425 (GRCm39)	nonsense	probably null
R5696:Zfp1005	UTSW	2	150,111,394 (GRCm39)	missense	possibly damaging	0.52
R5697:Zfp1005	UTSW	2	150,111,394 (GRCm39)	missense	possibly damaging	0.52
R5698:Zfp1005	UTSW	2	150,111,394 (GRCm39)	missense	possibly damaging	0.52
R5769:Zfp1005	UTSW	2	150,110,198 (GRCm39)	missense	possibly damaging	0.87
R5780:Zfp1005	UTSW	2	150,108,139 (GRCm39)	missense	probably benign	0.05
R5973:Zfp1005	UTSW	2	150,109,855 (GRCm39)	missense	unknown
R6662:Zfp1005	UTSW	2	150,108,172 (GRCm39)	critical splice donor site	probably null
R6878:Zfp1005	UTSW	2	150,108,406 (GRCm39)	missense	possibly damaging	0.86
R7037:Zfp1005	UTSW	2	150,108,376 (GRCm39)	missense	possibly damaging	0.86
R7081:Zfp1005	UTSW	2	150,110,189 (GRCm39)	missense	possibly damaging	0.66
R7413:Zfp1005	UTSW	2	150,108,081 (GRCm39)	missense	possibly damaging	0.93
R7725:Zfp1005	UTSW	2	150,110,468 (GRCm39)	missense	unknown
R7781:Zfp1005	UTSW	2	150,109,577 (GRCm39)	missense	possibly damaging	0.53
R8197:Zfp1005	UTSW	2	150,109,577 (GRCm39)	missense	possibly damaging	0.53
R8355:Zfp1005	UTSW	2	150,109,876 (GRCm39)	missense	unknown
R8517:Zfp1005	UTSW	2	150,110,043 (GRCm39)	missense	probably benign	0.33
R8812:Zfp1005	UTSW	2	150,109,624 (GRCm39)	missense	possibly damaging	0.83
R9108:Zfp1005	UTSW	2	150,109,969 (GRCm39)	missense	possibly damaging	0.61
R9488:Zfp1005	UTSW	2	150,110,477 (GRCm39)	missense	unknown
R9499:Zfp1005	UTSW	2	150,109,856 (GRCm39)	missense	unknown
R9551:Zfp1005	UTSW	2	150,109,856 (GRCm39)	missense	unknown
R9567:Zfp1005	UTSW	2	150,109,517 (GRCm39)	missense	possibly damaging	0.53
R9646:Zfp1005	UTSW	2	150,110,104 (GRCm39)	missense	probably benign	0.43
R9709:Zfp1005	UTSW	2	150,110,305 (GRCm39)	missense	possibly damaging	0.47
R9719:Zfp1005	UTSW	2	150,111,304 (GRCm39)	missense	possibly damaging	0.74
R9779:Zfp1005	UTSW	2	150,108,064 (GRCm39)	missense	possibly damaging	0.92
X0022:Zfp1005	UTSW	2	150,109,578 (GRCm39)	missense	possibly damaging	0.53
Z1177:Zfp1005	UTSW	2	150,110,244 (GRCm39)	missense	possibly damaging	0.94
Z1177:Zfp1005	UTSW	2	150,110,237 (GRCm39)	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- ACCCAGCATGCTCATCATTTTC -3'
(R):5'- CTTCAGTGAACTTTACAACCAAGC -3'

Sequencing Primer
(F):5'- TCCTCTTCAAGGGGTGTAGAAATGAC -3'
(R):5'- AAGCCTGATTAATTCACCTCATTC -3'

Posted On

2015-05-15