Mutagenetix > Incidental Mutation

Incidental Mutation 'R0391:Pcdh20'

31724

Institutional Source

Beutler Lab

Gene Symbol

Pcdh20

Ensembl Gene

ENSMUSG00000050505

Gene Name

protocadherin 20

Synonyms

PCDH13, C630015B17Rik

MMRRC Submission

038597-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0391 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

88702183-88708832 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 88706104 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 399 (I399V)

Ref Sequence

ENSEMBL: ENSMUSP00000141860 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061628] [ENSMUST00000192557]

AlphaFold

Q8BIZ0

Predicted Effect

probably benign
Transcript: ENSMUST00000061628
AA Change: I399V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000054774
Gene: ENSMUSG00000050505
AA Change: I399V

Domain	Start	End	E-Value	Type
CA	108	208	9.02e-1	SMART
CA	233	319	6.63e-16	SMART
CA	343	425	9.94e-2	SMART
CA	455	534	7.48e-3	SMART
CA	558	638	5.46e-26	SMART
CA	662	741	7.2e-26	SMART
CA	768	852	9.6e-13	SMART
low complexity region	860	871	N/A	INTRINSIC
transmembrane domain	890	912	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000192557
AA Change: I399V

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000141860
Gene: ENSMUSG00000050505
AA Change: I399V

Domain	Start	End	E-Value	Type
CA	108	208	9.02e-1	SMART
CA	233	319	6.63e-16	SMART
CA	343	425	9.94e-2	SMART
CA	455	534	7.48e-3	SMART
CA	558	638	5.46e-26	SMART
CA	662	741	7.2e-26	SMART
CA	768	852	9.6e-13	SMART
low complexity region	860	871	N/A	INTRINSIC
transmembrane domain	890	912	N/A	INTRINSIC

Meta Mutation Damage Score

0.0960

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 95.9%
20x: 91.8%

Validation Efficiency

97% (97/100)

MGI Phenotype

FUNCTION: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein contains six extracellular cadherin domains, a transmembrane domain, and a cytoplasmic tail differing from those of the classical cadherins. The encoded protein may play a role in cell adhesion in the nervous system and has been shown to be specifically expressed in newly differentiated olfactory sensory neurons and their axons during development. In adult mice, the expression of this protein in the olfactory system is more restricted but shows a gender difference with higher expression in the male than in the female. [provided by RefSeq, Sep 2009]

Allele List at MGI

Other mutations in this stock

Total: 97 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9530002B09Rik	T	A	4: 122,594,970 (GRCm39)		probably benign	Het
Abcc2	G	A	19: 43,810,044 (GRCm39)		probably benign	Het
Abcc8	C	G	7: 45,771,597 (GRCm39)	G838A	probably damaging	Het
Akr1c21	G	A	13: 4,631,199 (GRCm39)	A245T	probably damaging	Het
Anapc15-ps	T	C	10: 95,509,139 (GRCm39)	E47G	probably damaging	Het
Apoa1	A	G	9: 46,141,140 (GRCm39)	T79A	probably benign	Het
Atp6v1b1	A	G	6: 83,733,903 (GRCm39)	H378R	possibly damaging	Het
C4b	A	G	17: 34,954,588 (GRCm39)		probably benign	Het
Catsperd	A	T	17: 56,969,821 (GRCm39)	E638D	probably benign	Het
Cckar	C	T	5: 53,863,595 (GRCm39)		probably null	Het
Cfap100	C	T	6: 90,382,321 (GRCm39)		probably benign	Het
Chd1	G	T	17: 15,970,156 (GRCm39)	G970C	probably damaging	Het
Col14a1	A	G	15: 55,309,655 (GRCm39)		probably benign	Het
Col17a1	C	T	19: 47,652,263 (GRCm39)	V698M	probably damaging	Het
Cpeb1	T	C	7: 81,011,473 (GRCm39)	D156G	possibly damaging	Het
Cryl1	A	G	14: 57,541,232 (GRCm39)	Y151H	possibly damaging	Het
Csmd3	C	A	15: 47,520,969 (GRCm39)	V1881L	probably damaging	Het
Ctnnal1	C	T	4: 56,847,921 (GRCm39)	A73T	probably damaging	Het
Cyp2c37	T	C	19: 39,982,950 (GRCm39)	S180P	probably damaging	Het
Cyp2c54	T	C	19: 40,060,613 (GRCm39)	T123A	possibly damaging	Het
Dennd6b	T	C	15: 89,071,417 (GRCm39)	D304G	probably damaging	Het
Dnmt3l	T	C	10: 77,887,750 (GRCm39)		probably benign	Het
Eci1	G	A	17: 24,652,234 (GRCm39)		probably null	Het
Efhc1	A	G	1: 21,030,412 (GRCm39)	Y115C	probably damaging	Het
Ern1	T	A	11: 106,298,004 (GRCm39)	K706*	probably null	Het
Ghrl	T	C	6: 113,696,299 (GRCm39)	E31G	probably damaging	Het
Gpr108	A	C	17: 57,550,101 (GRCm39)	V179G	probably benign	Het
Henmt1	A	G	3: 108,865,851 (GRCm39)		probably benign	Het
Ift172	A	G	5: 31,444,011 (GRCm39)	V69A	probably damaging	Het
Il17ra	T	C	6: 120,453,940 (GRCm39)		probably benign	Het
Il17rb	T	C	14: 29,726,304 (GRCm39)	N95D	probably benign	Het
Il17rb	G	T	14: 29,728,112 (GRCm39)		probably null	Het
Iqub	G	A	6: 24,446,154 (GRCm39)	L757F	probably benign	Het
Itpr1	T	C	6: 108,355,128 (GRCm39)	V473A	probably benign	Het
Itpr2	T	G	6: 146,131,271 (GRCm39)	N1978H	probably damaging	Het
Klk1b26	T	A	7: 43,662,151 (GRCm39)	F3Y	probably damaging	Het
Lars1	A	G	18: 42,384,428 (GRCm39)	V50A	probably benign	Het
Lax1	G	T	1: 133,607,804 (GRCm39)	H312Q	probably benign	Het
Lctl	T	C	9: 64,029,596 (GRCm39)		probably benign	Het
Lrp2	T	A	2: 69,287,202 (GRCm39)	D3745V	probably damaging	Het
Lrp2	G	A	2: 69,290,681 (GRCm39)		probably benign	Het
Lvrn	A	T	18: 46,983,533 (GRCm39)	H92L	probably benign	Het
Marchf1	A	G	8: 66,871,625 (GRCm39)	T385A	probably damaging	Het
Marf1	C	T	16: 13,960,398 (GRCm39)	A549T	probably damaging	Het
Mbd5	T	C	2: 49,162,428 (GRCm39)	V970A	possibly damaging	Het
Mccc1	A	G	3: 36,017,719 (GRCm39)		probably benign	Het
Mpp4	A	T	1: 59,182,988 (GRCm39)		probably benign	Het
Mrnip	G	A	11: 50,090,747 (GRCm39)	A304T	probably damaging	Het
Muc5b	T	C	7: 141,418,819 (GRCm39)	S3922P	possibly damaging	Het
Myh3	T	A	11: 66,987,333 (GRCm39)		probably benign	Het
Nbea	A	T	3: 55,944,698 (GRCm39)	H555Q	probably damaging	Het
Niban3	T	A	8: 72,055,143 (GRCm39)		probably benign	Het
Nlrp9c	A	T	7: 26,070,901 (GRCm39)		probably benign	Het
Nmur1	A	T	1: 86,315,400 (GRCm39)	V178E	probably damaging	Het
Nod2	T	G	8: 89,390,406 (GRCm39)	S238A	probably benign	Het
Ogfod1	A	T	8: 94,789,651 (GRCm39)	T451S	probably damaging	Het
Or1e17	T	C	11: 73,831,935 (GRCm39)	F288L	probably damaging	Het
Or2d36	T	A	7: 106,747,394 (GRCm39)	Y290*	probably null	Het
Or2z8	C	T	8: 72,812,244 (GRCm39)	T240M	probably damaging	Het
Or8b8	G	A	9: 37,809,138 (GRCm39)	G146D	probably benign	Het
Pdlim1	G	T	19: 40,232,017 (GRCm39)	H120Q	probably damaging	Het
Plg	T	C	17: 12,637,968 (GRCm39)	V798A	probably damaging	Het
Polr2c	A	G	8: 95,584,403 (GRCm39)	I39V	possibly damaging	Het
Ppfia2	C	A	10: 106,666,575 (GRCm39)		probably benign	Het
Ppp1r3a	A	T	6: 14,719,696 (GRCm39)	I406N	probably benign	Het
Psg28	A	T	7: 18,160,098 (GRCm39)	M366K	probably benign	Het
Rad54b	T	C	4: 11,601,702 (GRCm39)	I419T	probably damaging	Het
Rnf43	A	G	11: 87,622,108 (GRCm39)	Q403R	possibly damaging	Het
Sema6a	G	A	18: 47,423,112 (GRCm39)		probably null	Het
Slc28a3	A	G	13: 58,717,229 (GRCm39)		probably benign	Het
Smad2	A	T	18: 76,422,108 (GRCm39)		probably null	Het
Smad4	G	A	18: 73,791,720 (GRCm39)	P274S	probably benign	Het
Smchd1	A	T	17: 71,710,149 (GRCm39)	V906D	probably damaging	Het
Soat2	C	A	15: 102,067,188 (GRCm39)	R320S	possibly damaging	Het
Spata33	C	T	8: 123,948,626 (GRCm39)	A57V	probably damaging	Het
Stab1	A	G	14: 30,865,375 (GRCm39)	L1814P	probably benign	Het
Stab2	T	C	10: 86,783,008 (GRCm39)	K680R	probably benign	Het
Stil	A	G	4: 114,898,369 (GRCm39)		probably null	Het
Sympk	T	A	7: 18,780,774 (GRCm39)	L759H	probably benign	Het
Tet1	A	T	10: 62,650,325 (GRCm39)		probably null	Het
Tfpi2	A	T	6: 3,965,460 (GRCm39)	N117K	probably benign	Het
Tle3	A	G	9: 61,323,943 (GRCm39)	Y766C	probably damaging	Het
Trpt1	C	A	19: 6,975,298 (GRCm39)		probably null	Het
Tshz1	A	G	18: 84,034,174 (GRCm39)	F78S	possibly damaging	Het
Ttc1	T	C	11: 43,629,635 (GRCm39)	D177G	probably damaging	Het
Ttc13	T	A	8: 125,401,140 (GRCm39)	Y741F	probably damaging	Het
Ulk3	C	T	9: 57,502,115 (GRCm39)	S462L	probably benign	Het
Utrn	C	T	10: 12,401,077 (GRCm39)		probably benign	Het
V1rd19	A	C	7: 23,703,010 (GRCm39)	T159P	probably damaging	Het
Vars1	T	C	17: 35,230,462 (GRCm39)	V515A	possibly damaging	Het
Vmn1r85	A	G	7: 12,818,515 (GRCm39)	Y210H	probably benign	Het
Vmn2r89	A	G	14: 51,693,435 (GRCm39)	T262A	probably damaging	Het
Vps53	G	A	11: 76,012,405 (GRCm39)	T209I	probably benign	Het
Wdfy2	T	C	14: 63,162,582 (GRCm39)	F95L	possibly damaging	Het
Wwp1	G	T	4: 19,627,911 (GRCm39)	S694Y	probably damaging	Het
Zbtb8b	T	A	4: 129,326,463 (GRCm39)	D201V	probably damaging	Het
Zmym5	A	C	14: 57,041,908 (GRCm39)	N123K	possibly damaging	Het

Other mutations in Pcdh20

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00566:Pcdh20	APN	14	88,705,317 (GRCm39)	missense	possibly damaging	0.65
IGL01112:Pcdh20	APN	14	88,704,636 (GRCm39)	missense	probably benign	0.01
IGL01586:Pcdh20	APN	14	88,708,344 (GRCm39)	missense	probably benign	0.37
IGL02007:Pcdh20	APN	14	88,707,031 (GRCm39)	missense	probably benign	0.09
IGL02545:Pcdh20	APN	14	88,706,280 (GRCm39)	missense	possibly damaging	0.74
IGL02935:Pcdh20	APN	14	88,704,438 (GRCm39)	utr 3 prime	probably benign
PIT4362001:Pcdh20	UTSW	14	88,704,462 (GRCm39)	missense	probably damaging	0.97
PIT4403001:Pcdh20	UTSW	14	88,704,462 (GRCm39)	missense	probably damaging	0.97
PIT4453001:Pcdh20	UTSW	14	88,704,744 (GRCm39)	missense	probably damaging	1.00
R0322:Pcdh20	UTSW	14	88,706,383 (GRCm39)	missense	probably benign	0.24
R0372:Pcdh20	UTSW	14	88,706,439 (GRCm39)	missense	probably damaging	1.00
R0575:Pcdh20	UTSW	14	88,705,048 (GRCm39)	missense	probably damaging	1.00
R0789:Pcdh20	UTSW	14	88,706,226 (GRCm39)	missense	probably damaging	1.00
R1370:Pcdh20	UTSW	14	88,705,737 (GRCm39)	missense	probably benign	0.00
R1465:Pcdh20	UTSW	14	88,706,673 (GRCm39)	missense	probably benign	0.00
R1465:Pcdh20	UTSW	14	88,706,673 (GRCm39)	missense	probably benign	0.00
R1664:Pcdh20	UTSW	14	88,705,758 (GRCm39)	missense	possibly damaging	0.63
R1677:Pcdh20	UTSW	14	88,705,410 (GRCm39)	missense	probably damaging	1.00
R1764:Pcdh20	UTSW	14	88,706,620 (GRCm39)	missense	possibly damaging	0.77
R1907:Pcdh20	UTSW	14	88,706,140 (GRCm39)	missense	probably benign	0.01
R2043:Pcdh20	UTSW	14	88,704,591 (GRCm39)	missense	probably benign	0.01
R2430:Pcdh20	UTSW	14	88,704,984 (GRCm39)	missense	probably damaging	1.00
R2471:Pcdh20	UTSW	14	88,704,672 (GRCm39)	missense	probably benign	0.00
R3838:Pcdh20	UTSW	14	88,705,899 (GRCm39)	missense	probably benign	0.00
R4163:Pcdh20	UTSW	14	88,705,615 (GRCm39)	missense	probably damaging	1.00
R4472:Pcdh20	UTSW	14	88,706,434 (GRCm39)	missense	probably benign	0.21
R4602:Pcdh20	UTSW	14	88,705,866 (GRCm39)	missense	probably damaging	1.00
R4681:Pcdh20	UTSW	14	88,705,052 (GRCm39)	missense	probably damaging	1.00
R4918:Pcdh20	UTSW	14	88,705,104 (GRCm39)	missense	probably damaging	1.00
R4921:Pcdh20	UTSW	14	88,707,162 (GRCm39)	missense	probably benign	0.01
R5204:Pcdh20	UTSW	14	88,706,351 (GRCm39)	missense	probably damaging	1.00
R5256:Pcdh20	UTSW	14	88,705,813 (GRCm39)	missense	probably benign
R5652:Pcdh20	UTSW	14	88,704,760 (GRCm39)	missense	probably damaging	1.00
R5815:Pcdh20	UTSW	14	88,708,312 (GRCm39)	missense	probably benign	0.03
R6195:Pcdh20	UTSW	14	88,705,488 (GRCm39)	missense	probably benign	0.39
R6233:Pcdh20	UTSW	14	88,705,488 (GRCm39)	missense	probably benign	0.39
R6848:Pcdh20	UTSW	14	88,704,690 (GRCm39)	missense	probably benign	0.03
R6913:Pcdh20	UTSW	14	88,706,038 (GRCm39)	missense	probably benign
R7044:Pcdh20	UTSW	14	88,706,607 (GRCm39)	missense	probably damaging	0.98
R7224:Pcdh20	UTSW	14	88,706,511 (GRCm39)	missense	possibly damaging	0.85
R7388:Pcdh20	UTSW	14	88,706,103 (GRCm39)	missense	probably benign	0.33
R7486:Pcdh20	UTSW	14	88,706,050 (GRCm39)	missense	possibly damaging	0.79
R7651:Pcdh20	UTSW	14	88,706,589 (GRCm39)	missense	probably damaging	1.00
R7664:Pcdh20	UTSW	14	88,706,803 (GRCm39)	missense	probably benign
R7706:Pcdh20	UTSW	14	88,704,793 (GRCm39)	missense	probably damaging	1.00
R7832:Pcdh20	UTSW	14	88,707,143 (GRCm39)	missense	probably null	0.02
R7892:Pcdh20	UTSW	14	88,704,867 (GRCm39)	nonsense	probably null
R8218:Pcdh20	UTSW	14	88,706,047 (GRCm39)	missense	probably damaging	0.96
R8545:Pcdh20	UTSW	14	88,706,601 (GRCm39)	missense	probably damaging	0.96
R8701:Pcdh20	UTSW	14	88,705,849 (GRCm39)	missense	possibly damaging	0.95
R9219:Pcdh20	UTSW	14	88,706,255 (GRCm39)	missense	probably benign	0.01
R9238:Pcdh20	UTSW	14	88,706,190 (GRCm39)	missense	probably benign	0.43
R9418:Pcdh20	UTSW	14	88,705,455 (GRCm39)	missense	probably benign
R9665:Pcdh20	UTSW	14	88,707,162 (GRCm39)	missense	probably benign	0.44
X0028:Pcdh20	UTSW	14	88,705,053 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGGCTCCAGTTCTTTCAGGTACAC -3'
(R):5'- CCAGGACCAGTATGTGAGCATCATC -3'

Sequencing Primer
(F):5'- GTTCTTTCAGGTACACAACACC -3'
(R):5'- CACCGTTTATGGGAATGCTACAG -3'

Posted On

2013-04-24