Mutagenetix > Incidental Mutation

Incidental Mutation 'R4087:Sertad2'

317426

Institutional Source

Beutler Lab

Gene Symbol

Sertad2

Ensembl Gene

ENSMUSG00000049800

Gene Name

SERTA domain containing 2

Synonyms

SEI-2, Trip-Br2, Sei2

MMRRC Submission

040980-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.483) question?

Stock #

R4087 (G1)

Quality Score

153

Status

Validated

Chromosome

Chromosomal Location

20493253-20603021 bp(+) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

GCCCC to GCCCCC at 20598664 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000105215 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000093292] [ENSMUST00000109585] [ENSMUST00000109586]

AlphaFold

Q9JJG5

Predicted Effect

probably null
Transcript: ENSMUST00000093292
AA Change: 288

SMART Domains

Protein: ENSMUSP00000090981
Gene: ENSMUSG00000049800
AA Change: 288

Domain	Start	End	E-Value	Type
Pfam:SERTA	40	77	1.6e-20	PFAM
low complexity region	89	99	N/A	INTRINSIC
low complexity region	175	189	N/A	INTRINSIC
low complexity region	226	248	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000109585
AA Change: 288

SMART Domains

Protein: ENSMUSP00000105214
Gene: ENSMUSG00000049800
AA Change: 288

Domain	Start	End	E-Value	Type
Pfam:SERTA	40	77	5.3e-20	PFAM
low complexity region	89	99	N/A	INTRINSIC
low complexity region	175	189	N/A	INTRINSIC
low complexity region	226	248	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000109586

SMART Domains

Protein: ENSMUSP00000105215
Gene: ENSMUSG00000049800

Domain	Start	End	E-Value	Type
Pfam:SERTA	40	77	5.3e-20	PFAM
low complexity region	89	99	N/A	INTRINSIC
low complexity region	175	189	N/A	INTRINSIC
low complexity region	226	248	N/A	INTRINSIC

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.7%
20x: 96.3%

Validation Efficiency

96% (50/52)

MGI Phenotype

PHENOTYPE: Mice homozygous for a gene trap allele exhibit enhanced lipolysis, thermogenesis, and oxidative metabolism with resistance to diet induced obesity and steatosis and improved when fed a high fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A530064D06Rik	A	G	17: 48,473,678 (GRCm39)	S80P	probably damaging	Het
Acss3	T	G	10: 106,889,313 (GRCm39)	Y169S	probably damaging	Het
Cep250	G	A	2: 155,834,552 (GRCm39)	R2159K	probably damaging	Het
Cldn34c4	A	T	X: 126,629,011 (GRCm39)	V153E	probably damaging	Het
Col4a4	T	A	1: 82,501,643 (GRCm39)	Y370F	unknown	Het
Col6a6	A	T	9: 105,661,155 (GRCm39)	I318N	possibly damaging	Het
Csmd1	T	A	8: 16,042,738 (GRCm39)	I2332F	probably damaging	Het
Dnajc28	G	A	16: 91,413,755 (GRCm39)	T187M	probably damaging	Het
Dym	T	A	18: 75,363,172 (GRCm39)	Y559N	probably damaging	Het
Eif3g	A	G	9: 20,809,248 (GRCm39)	V59A	possibly damaging	Het
Fam171a1	G	A	2: 3,227,333 (GRCm39)	R697Q	probably damaging	Het
Fermt3	T	A	19: 6,980,945 (GRCm39)		probably null	Het
Git2	A	G	5: 114,902,466 (GRCm39)	Y189H	probably damaging	Het
Gkn3	C	T	6: 87,360,507 (GRCm39)	A163T	probably damaging	Het
Gm7713	C	T	15: 59,866,258 (GRCm39)		noncoding transcript	Het
Gpr108	A	G	17: 57,544,925 (GRCm39)	Y313H	probably damaging	Het
Itprid2	C	T	2: 79,488,691 (GRCm39)	Q925*	probably null	Het
Kcnh8	T	C	17: 53,110,428 (GRCm39)	I213T	possibly damaging	Het
Lpgat1	A	T	1: 191,495,728 (GRCm39)	I306F	possibly damaging	Het
Mapk8	T	C	14: 33,112,205 (GRCm39)	T228A	probably benign	Het
Med12l	T	C	3: 59,205,342 (GRCm39)	V2101A	probably benign	Het
Mettl13	T	C	1: 162,375,771 (GRCm39)	K19E	possibly damaging	Het
Mta1	A	G	12: 113,075,802 (GRCm39)	Y22C	probably damaging	Het
Notch3	T	C	17: 32,377,087 (GRCm39)	T273A	possibly damaging	Het
Notch4	T	C	17: 34,803,409 (GRCm39)	W1443R	probably damaging	Het
Npy5r	T	A	8: 67,134,697 (GRCm39)	D32V	probably damaging	Het
Or8k36-ps1	C	A	2: 86,437,297 (GRCm39)	*206L	probably null	Het
Rbm47	A	G	5: 66,180,080 (GRCm39)	M409T	probably benign	Het
Rnf144a	C	T	12: 26,377,591 (GRCm39)	V51I	probably damaging	Het
Rxfp1	T	A	3: 79,552,256 (GRCm39)	T682S	probably damaging	Het
Sos1	A	G	17: 80,756,781 (GRCm39)	V257A	probably benign	Het
Tdrd9	C	T	12: 111,979,920 (GRCm39)	Q256*	probably null	Het
Tmprss11d	A	T	5: 86,457,138 (GRCm39)	S174T	probably damaging	Het
Tor1b	T	A	2: 30,846,531 (GRCm39)	I238N	probably damaging	Het
Tppp2	T	C	14: 52,156,957 (GRCm39)		probably null	Het
Traf3ip3	A	G	1: 192,863,628 (GRCm39)	V414A	probably damaging	Het
Trim14	T	A	4: 46,523,709 (GRCm39)	T110S	probably benign	Het
Trim30d	T	C	7: 104,137,007 (GRCm39)	N66D	probably damaging	Het
Usp48	A	G	4: 137,350,651 (GRCm39)	N46S	possibly damaging	Het
Vmn2r115	A	C	17: 23,565,358 (GRCm39)	Q415P	probably benign	Het
Wbp2nl	A	G	15: 82,192,762 (GRCm39)	M149V	probably benign	Het
Zfp106	T	C	2: 120,357,380 (GRCm39)		probably null	Het
Zfp281	T	A	1: 136,553,859 (GRCm39)	I279N	probably damaging	Het

Other mutations in Sertad2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03008:Sertad2	APN	11	20,597,798 (GRCm39)	splice site	probably benign
wisteria	UTSW	11	20,598,664 (GRCm39)	frame shift	probably null
PIT4366001:Sertad2	UTSW	11	20,598,116 (GRCm39)	missense	probably benign	0.03
R1171:Sertad2	UTSW	11	20,598,091 (GRCm39)	missense	probably benign	0.01
R1306:Sertad2	UTSW	11	20,598,388 (GRCm39)	missense	probably benign
R3834:Sertad2	UTSW	11	20,598,482 (GRCm39)	missense	probably benign	0.15
R4940:Sertad2	UTSW	11	20,597,899 (GRCm39)	missense	possibly damaging	0.52
R5232:Sertad2	UTSW	11	20,598,344 (GRCm39)	missense	possibly damaging	0.72
R5621:Sertad2	UTSW	11	20,598,061 (GRCm39)	missense	possibly damaging	0.61
R5891:Sertad2	UTSW	11	20,597,884 (GRCm39)	missense	probably benign	0.30
R5956:Sertad2	UTSW	11	20,597,884 (GRCm39)	missense	probably benign	0.30
R6006:Sertad2	UTSW	11	20,597,884 (GRCm39)	missense	probably benign	0.30
R6007:Sertad2	UTSW	11	20,597,884 (GRCm39)	missense	probably benign	0.30
R6048:Sertad2	UTSW	11	20,598,436 (GRCm39)	missense	probably benign
R9135:Sertad2	UTSW	11	20,598,425 (GRCm39)	missense	probably benign	0.00
R9136:Sertad2	UTSW	11	20,598,425 (GRCm39)	missense	probably benign	0.00
R9137:Sertad2	UTSW	11	20,598,425 (GRCm39)	missense	probably benign	0.00
R9138:Sertad2	UTSW	11	20,598,425 (GRCm39)	missense	probably benign	0.00
R9431:Sertad2	UTSW	11	20,598,425 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GGGTTTCCTGACAGACTTGAC -3'
(R):5'- AAGGCACTCCAACTCTGCTTG -3'

Sequencing Primer
(F):5'- AGACTTGACCCTGGATGACATCTTG -3'
(R):5'- GCTTGAAGAAAATACTAGTGTGATCC -3'

Posted On

2015-05-15