Mutagenetix > Incidental Mutations

Incidental Mutation 'R4088:Zfp9'

317465

Institutional Source

Beutler Lab

Gene Symbol

Zfp9

Ensembl Gene

ENSMUSG00000072623

Gene Name

zinc finger protein 9

Synonyms

Krox-4, 1810048F22Rik, Zfp-9

MMRRC Submission

040981-MU

Accession Numbers

Is this an essential gene?

Possibly essential (E-score: 0.533) question?

Stock #

R4088 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

118461950-118479320 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 118464769 bp

Zygosity

Heterozygous

Amino Acid Change

Proline to Threonine at position 311 (P311T)

Ref Sequence

ENSEMBL: ENSMUSP00000123810 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000161170]

Predicted Effect

probably damaging
Transcript: ENSMUST00000161170
AA Change: P311T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000123810
Gene: ENSMUSG00000072623
AA Change: P311T

Domain	Start	End	E-Value	Type
KRAB	8	68	2.26e-31	SMART
ZnF_C2H2	117	139	1.82e-3	SMART
ZnF_C2H2	144	166	4.17e-3	SMART
ZnF_C2H2	172	194	5.99e-4	SMART
ZnF_C2H2	200	222	2.79e-4	SMART
ZnF_C2H2	228	250	1.56e-2	SMART
ZnF_C2H2	256	278	6.88e-4	SMART
ZnF_C2H2	284	306	7.9e-4	SMART
ZnF_C2H2	312	334	2.75e-3	SMART
ZnF_C2H2	340	362	5.14e-3	SMART
ZnF_C2H2	368	390	1.26e-2	SMART
ZnF_C2H2	396	418	1.6e-4	SMART
ZnF_C2H2	424	446	9.08e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204066

Meta Mutation Damage Score

0.1784

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.5%

Validation Efficiency

96% (55/57)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430110L20Rik	T	C	1: 181,227,709		noncoding transcript	Het
Alox12b	G	T	11: 69,158,385	V87L	probably benign	Het
Arsk	T	G	13: 76,098,414	N32T	probably benign	Het
Baz1b	T	A	5: 135,216,940	N414K	probably damaging	Het
Cacna1e	A	T	1: 154,412,183		probably null	Het
Cdc73	A	G	1: 143,608,514		probably benign	Het
Cdhr2	A	G	13: 54,717,888	D233G	probably null	Het
Cep250	G	A	2: 155,992,632	R2159K	probably damaging	Het
Col4a4	T	A	1: 82,523,922	Y370F	unknown	Het
Cyp2b9	T	C	7: 26,173,456	L8P	probably damaging	Het
Ddx4	A	G	13: 112,613,761	V386A	probably benign	Het
Dnajc28	G	A	16: 91,616,867	T187M	probably damaging	Het
Dnajc6	T	A	4: 101,639,396	V920E	probably damaging	Het
Eef2kmt	A	T	16: 5,253,035	I50N	probably damaging	Het
Esyt3	T	C	9: 99,324,237	M40V	probably benign	Het
Gkn3	C	T	6: 87,383,525	A163T	probably damaging	Het
Gpr26	G	T	7: 131,967,076	C50F	probably benign	Het
Hmcn1	A	G	1: 150,703,216	I1993T	possibly damaging	Het
Hsdl2	T	A	4: 59,610,636	L211Q	unknown	Het
Khdrbs2	A	G	1: 32,333,524	E93G	probably damaging	Het
Kif13b	T	C	14: 64,767,455		probably null	Het
Kif15	T	A	9: 122,986,189	L423I	probably benign	Het
Kmt2c	A	G	5: 25,287,713	V834A	probably benign	Het
Lama3	T	A	18: 12,504,308	Y28*	probably null	Het
Map4k2	A	T	19: 6,353,156	H763L	probably damaging	Het
Mkl2	T	C	16: 13,384,200	F120S	probably damaging	Het
Ndufs3	C	T	2: 90,898,345		probably benign	Het
Nfasc	A	T	1: 132,595,591	V887E	probably damaging	Het
Npsr1	G	A	9: 24,313,769	E358K	possibly damaging	Het
Nrcam	T	C	12: 44,572,202	L828P	possibly damaging	Het
Olfr344	T	A	2: 36,569,018	L140H	probably damaging	Het
Oprm1	T	C	10: 6,830,234	Y299H	probably damaging	Het
Osbpl8	T	C	10: 111,289,790	V777A	possibly damaging	Het
Pcnt	T	C	10: 76,428,014	E372G	probably damaging	Het
Plekhh2	T	C	17: 84,617,999	V1408A	probably benign	Het
Sbk2	T	C	7: 4,957,628	Y181C	probably damaging	Het
Sf1	G	A	19: 6,368,440		probably null	Het
Slc26a9	A	G	1: 131,767,849	E765G	possibly damaging	Het
Stab2	A	G	10: 86,922,185	L1073P	probably damaging	Het
Sypl2	A	G	3: 108,217,676	I123T	possibly damaging	Het
Taar3	A	G	10: 23,949,859	D101G	possibly damaging	Het
Thsd4	T	C	9: 59,997,222	R204G	probably benign	Het
Traf3ip3	A	G	1: 193,181,320	V414A	probably damaging	Het
Trim14	T	A	4: 46,523,709	T110S	probably benign	Het
Trim65	G	A	11: 116,126,479	Q386*	probably null	Het
Ttn	G	A	2: 76,917,770	Q4312*	probably null	Het
Ubtfl1	T	C	9: 18,409,968	V264A	probably damaging	Het
Vmn1r58	C	A	7: 5,410,655	W192L	probably damaging	Het
Washc5	C	A	15: 59,339,862	C969F	probably damaging	Het
Zdhhc14	A	G	17: 5,726,856	K296R	probably benign	Het
Zfp281	T	A	1: 136,626,121	I279N	probably damaging	Het

Other mutations in Zfp9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00336:Zfp9	APN	6	118464475	missense	probably damaging	1.00
IGL01093:Zfp9	APN	6	118465839	missense	probably benign	0.14
IGL01512:Zfp9	APN	6	118467331	missense	probably damaging	1.00
R0491:Zfp9	UTSW	6	118465202	missense	probably damaging	0.96
R1716:Zfp9	UTSW	6	118464751	missense	probably damaging	1.00
R1858:Zfp9	UTSW	6	118465060	missense	probably benign	0.15
R4490:Zfp9	UTSW	6	118465312	missense	probably benign	0.06
R4627:Zfp9	UTSW	6	118464976	missense	probably damaging	1.00
R4951:Zfp9	UTSW	6	118464447	missense	probably damaging	1.00
R5344:Zfp9	UTSW	6	118465179	missense	probably damaging	1.00
R5594:Zfp9	UTSW	6	118465039	missense	probably damaging	0.98
R6198:Zfp9	UTSW	6	118477321	start codon destroyed	probably null
R6682:Zfp9	UTSW	6	118467241	missense	possibly damaging	0.82
R6986:Zfp9	UTSW	6	118464465	missense	possibly damaging	0.77
R7147:Zfp9	UTSW	6	118465002	missense	probably damaging	1.00
R7799:Zfp9	UTSW	6	118464882	missense	probably damaging	1.00
R7921:Zfp9	UTSW	6	118465071	missense	possibly damaging	0.70
R7934:Zfp9	UTSW	6	118464886	missense	probably damaging	0.99
R8111:Zfp9	UTSW	6	118464600	missense	probably damaging	1.00
R8369:Zfp9	UTSW	6	118464392	missense	probably damaging	0.99
R8916:Zfp9	UTSW	6	118465262	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACTTCTGGATAAAGCCTTCCC -3'
(R):5'- CACAGGGGAGAAGCCATTTG -3'

Sequencing Primer
(F):5'- TTCTGAGAGAAGGACTTCCCAC -3'
(R):5'- AGGCATACCTCCTGGTGCAC -3'

Posted On

2015-05-15