Mutagenetix > Incidental Mutation

Incidental Mutation 'R4093:Zfp64'

317708

Institutional Source

Beutler Lab

Gene Symbol

Zfp64

Ensembl Gene

ENSMUSG00000027551

Gene Name

zinc finger protein 64

Synonyms

MMRRC Submission

041627-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.828) question?

Stock #

R4093 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

168735251-168797507 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 168767855 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 586 (T586A)

Ref Sequence

ENSEMBL: ENSMUSP00000085285 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087971] [ENSMUST00000109162]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000087971
AA Change: T586A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000085285
Gene: ENSMUSG00000027551
AA Change: T586A

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART
ZnF_C2H2	257	279	3.89e-3	SMART
ZnF_C2H2	285	308	6.32e-3	SMART
ZnF_C2H2	313	335	2.05e-2	SMART
ZnF_C2H2	341	363	1.95e-3	SMART
ZnF_C2H2	369	392	1.36e-2	SMART
ZnF_C2H2	423	445	1.47e-3	SMART
low complexity region	503	522	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000109162

SMART Domains

Protein: ENSMUSP00000104790
Gene: ENSMUSG00000027551

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART
ZnF_C2H2	297	322	5.2e0	SMART
ZnF_C2H2	328	350	4.17e-3	SMART
ZnF_C2H2	356	378	6.78e-3	SMART
ZnF_C2H2	384	406	2.24e-3	SMART
ZnF_C2H2	412	434	1.79e-2	SMART
ZnF_C2H2	440	463	9.58e-3	SMART
ZnF_C2H2	465	487	3.78e-1	SMART
ZnF_C2H2	493	515	3.89e-3	SMART
ZnF_C2H2	521	544	1.92e-2	SMART
ZnF_C2H2	578	600	5.34e-1	SMART

Meta Mutation Damage Score

0.0934

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.5%

Validation Efficiency

92% (69/75)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap9	A	G	5: 4,093,996 (GRCm39)	M2173V	probably damaging	Het
Alas1	G	T	9: 106,119,000 (GRCm39)		probably null	Het
Angpt1	T	C	15: 42,386,941 (GRCm39)	T138A	probably damaging	Het
Atp2c2	G	A	8: 120,476,610 (GRCm39)		probably null	Het
Cadm2	A	G	16: 66,581,675 (GRCm39)	V210A	possibly damaging	Het
Cfap70	A	T	14: 20,459,181 (GRCm39)	D671E	probably damaging	Het
Chd2	C	T	7: 73,150,764 (GRCm39)	E322K	possibly damaging	Het
Chrnd	C	T	1: 87,118,729 (GRCm39)	Q29*	probably null	Het
Cip2a	T	C	16: 48,821,339 (GRCm39)		probably benign	Het
Cym	A	G	3: 107,121,582 (GRCm39)	S237P	probably benign	Het
D030068K23Rik	A	G	8: 109,978,091 (GRCm39)		noncoding transcript	Het
Gsdma2	T	A	11: 98,541,677 (GRCm39)	S135T	probably benign	Het
Hc	A	T	2: 34,873,819 (GRCm39)	Y158*	probably null	Het
Hoxb3	C	A	11: 96,236,926 (GRCm39)	H335N	probably damaging	Het
Htr2a	G	A	14: 74,943,789 (GRCm39)	M456I	probably benign	Het
Ighv1-19	G	A	12: 114,672,350 (GRCm39)	T90I	probably damaging	Het
Kansl3	C	A	1: 36,384,035 (GRCm39)	S729I	probably damaging	Het
Kcnab1	T	C	3: 65,207,035 (GRCm39)	I137T	possibly damaging	Het
Kcnt1	A	T	2: 25,767,927 (GRCm39)	E12V	probably damaging	Het
Klk15	A	T	7: 43,588,204 (GRCm39)	S171C	possibly damaging	Het
Lcn2	A	T	2: 32,277,728 (GRCm39)	M1K	probably null	Het
Lrig1	T	C	6: 94,590,559 (GRCm39)	D487G	probably benign	Het
Lrp8	T	A	4: 107,700,468 (GRCm39)	C135*	probably null	Het
Lrrc31	C	A	3: 30,749,671 (GRCm39)	S54I	probably damaging	Het
Ltbp4	A	G	7: 27,024,641 (GRCm39)	V663A	possibly damaging	Het
Med27	G	A	2: 29,267,920 (GRCm39)		probably benign	Het
Mical1	T	C	10: 41,362,933 (GRCm39)		probably benign	Het
Myt1	A	T	2: 181,453,191 (GRCm39)	M799L	probably damaging	Het
Nlrc4	T	C	17: 74,752,953 (GRCm39)	M477V	probably benign	Het
Npy4r	T	C	14: 33,869,098 (GRCm39)	I63M	probably benign	Het
Or2t43	A	T	11: 58,457,655 (GRCm39)	I172N	probably damaging	Het
Or52e15	C	T	7: 104,645,842 (GRCm39)	G90S	probably benign	Het
Or8b1b	T	A	9: 38,375,379 (GRCm39)	L14Q	probably null	Het
Or8s10	T	C	15: 98,335,563 (GRCm39)	L71P	probably damaging	Het
Piezo1	A	C	8: 123,227,899 (GRCm39)		probably null	Het
Ppp1r12c	T	C	7: 4,486,366 (GRCm39)	E601G	probably damaging	Het
Proser1	T	C	3: 53,387,133 (GRCm39)		probably null	Het
Psme2	A	T	14: 55,825,734 (GRCm39)	N143K	probably benign	Het
Pygo2	C	A	3: 89,340,571 (GRCm39)	P323Q	probably damaging	Het
Rab11b	T	C	17: 33,968,763 (GRCm39)	T77A	possibly damaging	Het
Recql5	A	G	11: 115,795,714 (GRCm39)	S190P	probably benign	Het
Serpina12	A	G	12: 104,004,183 (GRCm39)	F150L	probably damaging	Het
Sfswap	A	G	5: 129,637,805 (GRCm39)	S821G	possibly damaging	Het
Slc22a2	C	T	17: 12,831,281 (GRCm39)	T357M	probably damaging	Het
Spag6l	A	C	16: 16,646,888 (GRCm39)	N39K	probably benign	Het
Srl	A	G	16: 4,315,316 (GRCm39)	S109P	possibly damaging	Het
Tagap	A	T	17: 8,148,255 (GRCm39)	H152L	probably damaging	Het
Tbx3	T	A	5: 119,818,813 (GRCm39)	S463T	probably benign	Het
Tcaf2	A	G	6: 42,619,772 (GRCm39)	L85P	probably damaging	Het
Tenm4	A	T	7: 96,544,979 (GRCm39)	S2332C	probably damaging	Het
Trim60	A	T	8: 65,454,030 (GRCm39)	M73K	probably benign	Het
Trpc1	T	C	9: 95,588,918 (GRCm39)	T769A	probably benign	Het
Tubgcp4	T	A	2: 121,025,958 (GRCm39)	L601Q	probably benign	Het
Unc5d	T	C	8: 29,334,865 (GRCm39)	Y154C	possibly damaging	Het
Vmn1r185	A	T	7: 26,311,208 (GRCm39)	V99E	probably damaging	Het
Vmn1r57	G	T	7: 5,223,856 (GRCm39)	S127I	possibly damaging	Het
Vmn1r62	T	A	7: 5,678,943 (GRCm39)	M208K	probably damaging	Het
Vmn2r110	T	C	17: 20,803,642 (GRCm39)	D311G	possibly damaging	Het
Vmn2r48	C	A	7: 9,676,185 (GRCm39)	S432I	probably benign	Het
Vmn2r48	T	A	7: 9,676,186 (GRCm39)	S432C	probably damaging	Het
Vmn2r71	T	C	7: 85,270,442 (GRCm39)	V536A	probably benign	Het
Vstm2a	A	G	11: 16,209,884 (GRCm39)	T37A	probably damaging	Het
Wfs1	T	C	5: 37,124,809 (GRCm39)	H694R	probably damaging	Het
Zdbf2	T	C	1: 63,348,940 (GRCm39)	S2440P	possibly damaging	Het
Zfp61	A	G	7: 23,990,700 (GRCm39)		probably null	Het
Zfp943	T	A	17: 22,211,963 (GRCm39)	C350S	probably damaging	Het

Other mutations in Zfp64

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00155:Zfp64	APN	2	168,768,601 (GRCm39)	missense	probably benign	0.06
IGL01479:Zfp64	APN	2	168,793,599 (GRCm39)	missense	probably damaging	1.00
IGL02320:Zfp64	APN	2	168,768,118 (GRCm39)	missense	probably damaging	0.98
IGL03411:Zfp64	APN	2	168,793,462 (GRCm39)	splice site	probably null
PIT4362001:Zfp64	UTSW	2	168,767,735 (GRCm39)	missense	probably benign	0.23
R0033:Zfp64	UTSW	2	168,767,635 (GRCm39)	missense	possibly damaging	0.94
R0033:Zfp64	UTSW	2	168,767,635 (GRCm39)	missense	possibly damaging	0.94
R0230:Zfp64	UTSW	2	168,754,150 (GRCm39)	splice site	probably benign
R0325:Zfp64	UTSW	2	168,767,960 (GRCm39)	missense	probably benign	0.00
R0689:Zfp64	UTSW	2	168,777,121 (GRCm39)	splice site	probably benign
R1741:Zfp64	UTSW	2	168,768,238 (GRCm39)	missense	probably benign	0.05
R2054:Zfp64	UTSW	2	168,767,728 (GRCm39)	missense	probably damaging	1.00
R2133:Zfp64	UTSW	2	168,782,663 (GRCm39)	missense	probably damaging	1.00
R2254:Zfp64	UTSW	2	168,768,662 (GRCm39)	missense	probably damaging	0.96
R4094:Zfp64	UTSW	2	168,767,855 (GRCm39)	missense	probably benign	0.00
R4257:Zfp64	UTSW	2	168,768,298 (GRCm39)	missense	probably damaging	1.00
R4630:Zfp64	UTSW	2	168,768,463 (GRCm39)	missense	possibly damaging	0.94
R4642:Zfp64	UTSW	2	168,776,851 (GRCm39)	missense	probably benign	0.22
R4817:Zfp64	UTSW	2	168,768,032 (GRCm39)	missense	probably benign
R4880:Zfp64	UTSW	2	168,736,297 (GRCm39)	missense	probably damaging	1.00
R5051:Zfp64	UTSW	2	168,768,304 (GRCm39)	missense	probably damaging	0.98
R5155:Zfp64	UTSW	2	168,748,885 (GRCm39)	missense	probably benign	0.17
R5562:Zfp64	UTSW	2	168,767,642 (GRCm39)	missense	probably benign	0.00
R5619:Zfp64	UTSW	2	168,741,734 (GRCm39)	missense	probably damaging	0.99
R5619:Zfp64	UTSW	2	168,741,735 (GRCm39)	missense	probably damaging	0.97
R5620:Zfp64	UTSW	2	168,741,888 (GRCm39)	missense	possibly damaging	0.70
R5919:Zfp64	UTSW	2	168,768,419 (GRCm39)	missense	probably benign	0.05
R6156:Zfp64	UTSW	2	168,768,088 (GRCm39)	missense	probably benign	0.34
R6364:Zfp64	UTSW	2	168,754,186 (GRCm39)	missense	probably damaging	1.00
R6488:Zfp64	UTSW	2	168,777,129 (GRCm39)	critical splice donor site	probably null
R6512:Zfp64	UTSW	2	168,735,997 (GRCm39)	missense	probably benign	0.00
R6588:Zfp64	UTSW	2	168,768,827 (GRCm39)	missense	probably damaging	1.00
R7103:Zfp64	UTSW	2	168,768,357 (GRCm39)	missense	probably benign	0.00
R7313:Zfp64	UTSW	2	168,741,810 (GRCm39)	missense	probably damaging	1.00
R7470:Zfp64	UTSW	2	168,767,731 (GRCm39)	missense	probably damaging	1.00
R7529:Zfp64	UTSW	2	168,735,992 (GRCm39)	missense	probably benign
R7560:Zfp64	UTSW	2	168,767,585 (GRCm39)	missense	probably damaging	1.00
R7963:Zfp64	UTSW	2	168,793,538 (GRCm39)	missense	probably benign	0.32
R8037:Zfp64	UTSW	2	168,741,932 (GRCm39)	missense	probably damaging	1.00
R8038:Zfp64	UTSW	2	168,741,932 (GRCm39)	missense	probably damaging	1.00
R8825:Zfp64	UTSW	2	168,793,552 (GRCm39)	missense	probably benign
R8840:Zfp64	UTSW	2	168,768,635 (GRCm39)	missense	probably benign
R8891:Zfp64	UTSW	2	168,797,083 (GRCm39)	start codon destroyed	probably null	0.37
R9062:Zfp64	UTSW	2	168,767,747 (GRCm39)	missense	probably benign
R9592:Zfp64	UTSW	2	168,768,118 (GRCm39)	missense	probably damaging	1.00
R9681:Zfp64	UTSW	2	168,793,680 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCCTGCTGGGTAGAGAAGATAGAG -3'
(R):5'- CAGCTGCCGTCAACATTGTG -3'

Sequencing Primer
(F):5'- AGATAGAGGGTGCCGTGGTG -3'
(R):5'- TAGCCCAGACCCCAGAGGAG -3'

Posted On

2015-05-15