Incidental Mutation 'R4093:Trim60'
ID317739
Institutional Source Beutler Lab
Gene Symbol Trim60
Ensembl Gene ENSMUSG00000053490
Gene Nametripartite motif-containing 60
SynonymsRnf33, 2czf45
MMRRC Submission 041627-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.067) question?
Stock #R4093 (G1)
Quality Score225
Status Validated
Chromosome8
Chromosomal Location64999307-65018584 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 65001378 bp
ZygosityHeterozygous
Amino Acid Change Methionine to Lysine at position 73 (M73K)
Ref Sequence ENSEMBL: ENSMUSP00000040299 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000048565]
Predicted Effect probably benign
Transcript: ENSMUST00000048565
AA Change: M73K

PolyPhen 2 Score 0.055 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000040299
Gene: ENSMUSG00000053490
AA Change: M73K

DomainStartEndE-ValueType
RING 15 55 1.48e-7 SMART
low complexity region 81 90 N/A INTRINSIC
BBOX 91 132 3.12e-6 SMART
PRY 289 341 4.11e-15 SMART
Pfam:SPRY 344 459 2e-14 PFAM
Meta Mutation Damage Score 0.1232 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.5%
Validation Efficiency 92% (69/75)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Pseudogenes of this gene are located on more than six chromosomes including chromosome 4. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2013]
Allele List at MGI
Other mutations in this stock
Total: 66 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Akap9 A G 5: 4,043,996 M2173V probably damaging Het
Alas1 G T 9: 106,241,801 probably null Het
Angpt1 T C 15: 42,523,545 T138A probably damaging Het
Atp2c2 G A 8: 119,749,871 probably null Het
C330027C09Rik T C 16: 49,000,976 probably benign Het
Cadm2 A G 16: 66,784,788 V210A possibly damaging Het
Cfap70 A T 14: 20,409,113 D671E probably damaging Het
Chd2 C T 7: 73,501,016 E322K possibly damaging Het
Chrnd C T 1: 87,191,007 Q29* probably null Het
Cym A G 3: 107,214,266 S237P probably benign Het
D030068K23Rik A G 8: 109,251,459 noncoding transcript Het
Gsdma2 T A 11: 98,650,851 S135T probably benign Het
Hc A T 2: 34,983,807 Y158* probably null Het
Hoxb3 C A 11: 96,346,100 H335N probably damaging Het
Htr2a G A 14: 74,706,349 M456I probably benign Het
Ighv1-19 G A 12: 114,708,730 T90I probably damaging Het
Kansl3 C A 1: 36,344,954 S729I probably damaging Het
Kcnab1 T C 3: 65,299,614 I137T possibly damaging Het
Kcnt1 A T 2: 25,877,915 E12V probably damaging Het
Klk15 A T 7: 43,938,780 S171C possibly damaging Het
Lcn2 A T 2: 32,387,716 M1K probably null Het
Lrig1 T C 6: 94,613,578 D487G probably benign Het
Lrp8 T A 4: 107,843,271 C135* probably null Het
Lrrc31 C A 3: 30,695,522 S54I probably damaging Het
Ltbp4 A G 7: 27,325,216 V663A possibly damaging Het
Med27 G A 2: 29,377,908 probably benign Het
Mical1 T C 10: 41,486,937 probably benign Het
Myt1 A T 2: 181,811,398 M799L probably damaging Het
Nlrc4 T C 17: 74,445,958 M477V probably benign Het
Npy4r T C 14: 34,147,141 I63M probably benign Het
Olfr224 A T 11: 58,566,829 I172N probably damaging Het
Olfr282 T C 15: 98,437,682 L71P probably damaging Het
Olfr672 C T 7: 104,996,635 G90S probably benign Het
Olfr904 T A 9: 38,464,083 L14Q probably null Het
Piezo1 A C 8: 122,501,160 probably null Het
Ppp1r12c T C 7: 4,483,367 E601G probably damaging Het
Proser1 T C 3: 53,479,712 probably null Het
Psme2 A T 14: 55,588,277 N143K probably benign Het
Pygo2 C A 3: 89,433,264 P323Q probably damaging Het
Rab11b T C 17: 33,749,789 T77A possibly damaging Het
Recql5 A G 11: 115,904,888 S190P probably benign Het
Serpina12 A G 12: 104,037,924 F150L probably damaging Het
Sfswap A G 5: 129,560,741 S821G possibly damaging Het
Slc22a2 C T 17: 12,612,394 T357M probably damaging Het
Spag6l A C 16: 16,829,024 N39K probably benign Het
Srl A G 16: 4,497,452 S109P possibly damaging Het
Tagap A T 17: 7,929,423 H152L probably damaging Het
Tbx3 T A 5: 119,680,748 S463T probably benign Het
Tcaf2 A G 6: 42,642,838 L85P probably damaging Het
Tenm4 A T 7: 96,895,772 S2332C probably damaging Het
Trpc1 T C 9: 95,706,865 T769A probably benign Het
Tubgcp4 T A 2: 121,195,477 L601Q probably benign Het
Unc5d T C 8: 28,844,837 Y154C possibly damaging Het
Vmn1r185 A T 7: 26,611,783 V99E probably damaging Het
Vmn1r57 G T 7: 5,220,857 S127I possibly damaging Het
Vmn1r62 T A 7: 5,675,944 M208K probably damaging Het
Vmn2r110 T C 17: 20,583,380 D311G possibly damaging Het
Vmn2r48 C A 7: 9,942,258 S432I probably benign Het
Vmn2r48 T A 7: 9,942,259 S432C probably damaging Het
Vmn2r71 T C 7: 85,621,234 V536A probably benign Het
Vstm2a A G 11: 16,259,884 T37A probably damaging Het
Wfs1 T C 5: 36,967,465 H694R probably damaging Het
Zdbf2 T C 1: 63,309,781 S2440P possibly damaging Het
Zfp61 A G 7: 24,291,275 probably null Het
Zfp64 T C 2: 168,925,935 T586A probably benign Het
Zfp943 T A 17: 21,992,982 C350S probably damaging Het
Other mutations in Trim60
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00467:Trim60 APN 8 65000719 missense possibly damaging 0.61
IGL03145:Trim60 APN 8 65000572 missense probably damaging 0.99
R0054:Trim60 UTSW 8 65001321 missense probably benign 0.05
R0054:Trim60 UTSW 8 65001321 missense probably benign 0.05
R0080:Trim60 UTSW 8 65000599 missense probably damaging 1.00
R0244:Trim60 UTSW 8 65001048 missense probably benign 0.07
R0348:Trim60 UTSW 8 65001216 missense probably damaging 1.00
R1104:Trim60 UTSW 8 65001419 missense probably benign 0.04
R1615:Trim60 UTSW 8 65000510 nonsense probably null
R1667:Trim60 UTSW 8 65001464 missense probably benign 0.00
R1944:Trim60 UTSW 8 65001312 missense possibly damaging 0.95
R2009:Trim60 UTSW 8 65001323 missense probably damaging 0.99
R4400:Trim60 UTSW 8 65001212 nonsense probably null
R5171:Trim60 UTSW 8 65000524 missense probably benign 0.05
R5898:Trim60 UTSW 8 65000364 nonsense probably null
R6586:Trim60 UTSW 8 65000596 missense possibly damaging 0.61
R7012:Trim60 UTSW 8 65000391 missense possibly damaging 0.93
R7092:Trim60 UTSW 8 65001048 missense probably benign 0.07
R7274:Trim60 UTSW 8 65000481 missense possibly damaging 0.76
R7567:Trim60 UTSW 8 65001525 missense probably damaging 0.97
Predicted Primers PCR Primer
(F):5'- TGGCGCTCTCTAGGACTTTC -3'
(R):5'- TCCTCTCAGACCAATGGACTC -3'

Sequencing Primer
(F):5'- ACTTTCCTGTGATAAGAGCTGGC -3'
(R):5'- CTTGCAGGACAAATGTATCTGTTAC -3'
Posted On2015-05-15