|Institutional Source||Beutler Lab|
|Gene Name||angiopoietin 4|
|Is this an essential gene?||Probably non essential (E-score: 0.092)|
|Stock #||R4192 (G1)|
|Chromosomal Location||151911210-151945337 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to C at 151943318 bp|
|Amino Acid Change||Aspartic acid to Alanine at position 418 (D418A)|
|Ref Sequence||ENSEMBL: ENSMUSP00000028955 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000028955]|
|Predicted Effect||probably benign
AA Change: D418A
PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
AA Change: D418A
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.0898|
|Coding Region Coverage||
|Validation Efficiency||100% (40/40)|
|MGI Phenotype||FUNCTION: This gene is a member of the angiopoietin family of secreted growth factors comprising Angiopoietins-1, -2, and -3, all of which bind the TEK Receptor Tyrosine Kinase. Angiopoietin/TEK Receptor Tyrosine Kinase signaling is involved in survival and migration of endothelial cells and regulates vascular remodeling and maintenance of vascular integrity. Angiopoietin/TEK Receptor Tyrosine Kinase signaling is also required for lymphangiogenesis. Family members bind TEK Receptor Tyrosine Kinase as multimeric clusters but their oligomerization properties differ from one another and this is thought to provide a mechanistic basis for their distinct physiologic roles. [provided by RefSeq, Jul 2016]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Angpt4||
(F):5'- TAAAAGTCCCAGGAGCGGTG -3'
(R):5'- TCGGATACACTCACCTCCAG -3'
(F):5'- CCCCAGGGTGGCAAATAAG -3'
(R):5'- TCAGAACACATTCACCTCCAGATATC -3'