Mutagenetix > Incidental Mutation

Incidental Mutation 'R4206:Gpr149'

318906

Institutional Source

Beutler Lab

Gene Symbol

Gpr149

Ensembl Gene

ENSMUSG00000043441

Gene Name

G protein-coupled receptor 149

Synonyms

PGR10, R35, Ieda, 9630018L10Rik

MMRRC Submission

041035-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R4206 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

62529077-62605140 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 62604503 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Alanine at position 25 (D25A)

Ref Sequence

ENSEMBL: ENSMUSP00000060893 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058535]

AlphaFold

Q3UVY1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000058535
AA Change: D25A

PolyPhen 2 Score 0.923 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000060893
Gene: ENSMUSG00000043441
AA Change: D25A

Domain	Start	End	E-Value	Type
Pfam:7tm_1	52	363	7.2e-7	PFAM
coiled coil region	694	730	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149007

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.5%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Female mice homozygous for a knock-out allele exhibit increased fertility with increased litter size and frequency. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921509C19Rik	T	C	2: 151,473,515	K81R	probably benign	Het
Acacb	T	C	5: 114,213,651	F1150L	probably benign	Het
Acat3	A	G	17: 12,927,386	Y237H	possibly damaging	Het
Arfgap3	T	C	15: 83,322,668	T240A	probably benign	Het
Arl6ip1	AAAATAAATAAATAAATAAATAAATA	AAAATAAATAAATAAATAAATAAATAAATA	7: 118,121,899		probably benign	Het
Cabin1	T	C	10: 75,754,841	S23G	possibly damaging	Het
Clasp1	A	G	1: 118,578,906	N949S	probably damaging	Het
Csrp1	T	G	1: 135,745,327	C61G	probably damaging	Het
Dgka	A	T	10: 128,721,195	L637Q	probably damaging	Het
Dst	G	A	1: 34,212,247	R1801H	probably damaging	Het
Edil3	T	C	13: 89,180,278	S284P	probably damaging	Het
Eif2d	A	G	1: 131,154,363	Y64C	probably damaging	Het
Ell2	A	G	13: 75,761,948	D139G	probably damaging	Het
Fam187a	A	G	11: 102,886,212	R281G	probably damaging	Het
Gin1	A	G	1: 97,792,420	D380G	possibly damaging	Het
Gyg	A	G	3: 20,152,737	S90P	probably benign	Het
Hpx	C	T	7: 105,595,147	M190I	probably null	Het
Irf2bpl	C	T	12: 86,883,036	V288I	probably benign	Het
Lyst	A	G	13: 13,635,989	H748R	probably benign	Het
Mmrn1	G	A	6: 60,958,180	G220D	probably damaging	Het
Mpdz	A	T	4: 81,381,762	M333K	probably benign	Het
Muc5ac	C	T	7: 141,817,110	S2556F	possibly damaging	Het
Nfix	CAAAAA	CAAAA	8: 84,716,247		probably null	Het
Ntsr1	A	G	2: 180,500,752	D112G	probably damaging	Het
Olfr1410	A	C	1: 92,608,595	T253P	possibly damaging	Het
Olfr657	T	A	7: 104,636,149	N158K	possibly damaging	Het
Olfr8	T	C	10: 78,955,283	F26S	probably benign	Het
Parg	A	G	14: 32,254,536	K178R	probably benign	Het
Pde8b	C	A	13: 95,222,545	C90F	probably benign	Het
Pld1	G	A	3: 28,120,783	V857I	probably benign	Het
Rad54l2	A	C	9: 106,717,795	V321G	probably damaging	Het
Rcn2	A	G	9: 56,045,207	Y112C	possibly damaging	Het
Rnf123	A	T	9: 108,063,963	D639E	probably benign	Het
Rufy2	C	T	10: 63,004,772	Q441*	probably null	Het
Scgb2b12	T	C	7: 32,326,638	Y43C	probably damaging	Het
Scrn3	T	A	2: 73,319,501		probably null	Het
Shroom3	G	T	5: 92,943,086	V1151F	probably damaging	Het
Slc22a8	T	C	19: 8,608,233	S321P	probably benign	Het
Tex11	C	A	X: 100,933,415	A487S	possibly damaging	Het
Tex2	A	G	11: 106,567,572		probably benign	Het
Tmem2	G	T	19: 21,842,115	R1090L	probably damaging	Het
Trip13	A	G	13: 73,932,890	I119T	probably benign	Het
Tsku	C	T	7: 98,352,998	R42H	probably damaging	Het
Ttn	A	G	2: 76,772,567	I16691T	possibly damaging	Het
Tyr	A	G	7: 87,429,068	L528P	possibly damaging	Het
Ubxn2b	T	C	4: 6,204,565	V142A	probably damaging	Het
Uggt2	C	T	14: 119,049,262	D221N	probably damaging	Het
Wnt4	A	G	4: 137,296,343	K207R	possibly damaging	Het
Zfp608	T	C	18: 54,988,195	R107G	probably damaging	Het

Other mutations in Gpr149

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00330:Gpr149	APN	3	62530673	missense	probably damaging	1.00
IGL01339:Gpr149	APN	3	62604297	missense	probably damaging	1.00
IGL01399:Gpr149	APN	3	62604431	missense	probably damaging	1.00
IGL01954:Gpr149	APN	3	62530927	missense	probably benign	0.36
IGL02115:Gpr149	APN	3	62594915	missense	probably benign	0.02
IGL02218:Gpr149	APN	3	62530531	utr 3 prime	probably benign
IGL02592:Gpr149	APN	3	62603810	missense	possibly damaging	0.75
IGL03393:Gpr149	APN	3	62603945	missense	probably benign	0.15
R0578:Gpr149	UTSW	3	62602689	missense	possibly damaging	0.81
R1173:Gpr149	UTSW	3	62604467	missense	probably damaging	1.00
R1174:Gpr149	UTSW	3	62604467	missense	probably damaging	1.00
R1175:Gpr149	UTSW	3	62604467	missense	probably damaging	1.00
R1432:Gpr149	UTSW	3	62531018	missense	probably damaging	1.00
R1484:Gpr149	UTSW	3	62595171	missense	probably benign	0.00
R1972:Gpr149	UTSW	3	62530795	missense	probably benign	0.39
R1973:Gpr149	UTSW	3	62530795	missense	probably benign	0.39
R2180:Gpr149	UTSW	3	62604068	missense	probably damaging	1.00
R2241:Gpr149	UTSW	3	62604053	missense	probably benign	0.00
R3118:Gpr149	UTSW	3	62595022	missense	probably benign	0.00
R3547:Gpr149	UTSW	3	62595128	missense	probably benign	0.01
R3548:Gpr149	UTSW	3	62595128	missense	probably benign	0.01
R4332:Gpr149	UTSW	3	62604373	missense	possibly damaging	0.93
R4531:Gpr149	UTSW	3	62602678	missense	probably benign	0.00
R4557:Gpr149	UTSW	3	62530870	missense	probably damaging	1.00
R4557:Gpr149	UTSW	3	62604497	missense	probably benign	0.02
R4593:Gpr149	UTSW	3	62602730	intron	probably benign
R5397:Gpr149	UTSW	3	62530805	missense	probably damaging	1.00
R6592:Gpr149	UTSW	3	62530540	missense	probably benign	0.02
R6642:Gpr149	UTSW	3	62530574	missense	probably damaging	1.00
R6845:Gpr149	UTSW	3	62604521	missense	possibly damaging	0.58
R7303:Gpr149	UTSW	3	62595070	missense	possibly damaging	0.59
R7659:Gpr149	UTSW	3	62603835	missense	probably benign	0.01
R7682:Gpr149	UTSW	3	62530739	missense	probably damaging	1.00
R7803:Gpr149	UTSW	3	62530715	missense	probably damaging	1.00
R7904:Gpr149	UTSW	3	62594935	missense	probably benign	0.00
R7943:Gpr149	UTSW	3	62530711	missense	probably damaging	1.00
R8844:Gpr149	UTSW	3	62595151	missense	probably benign	0.05
R8919:Gpr149	UTSW	3	62531057	missense	probably damaging	1.00
R9043:Gpr149	UTSW	3	62603939	missense	probably damaging	1.00
R9209:Gpr149	UTSW	3	62603672	missense	probably benign	0.40
Z1177:Gpr149	UTSW	3	62603959	frame shift	probably null
Z1190:Gpr149	UTSW	3	62604551	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TTGGCCACTGTAAAACCATGAAG -3'
(R):5'- GGAGACATGTCTGGACCAAC -3'

Sequencing Primer
(F):5'- CCATGAAGATGGCCACGGAC -3'
(R):5'- GAGACATGTCTGGACCAACACATG -3'

Posted On

2015-06-10