Mutagenetix > Incidental Mutation

Incidental Mutation 'R4207:Vmn2r85'

318978

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r85

Ensembl Gene

ENSMUSG00000092048

Gene Name

vomeronasal 2, receptor 85

Synonyms

EG623734

MMRRC Submission

041036-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R4207 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

130417772-130429612 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 130418705 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Tryptophan at position 703 (C703W)

Ref Sequence

ENSEMBL: ENSMUSP00000128792 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171811]

AlphaFold

G3UW56

Predicted Effect

probably damaging
Transcript: ENSMUST00000171811
AA Change: C703W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000128792
Gene: ENSMUSG00000092048
AA Change: C703W

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	9e-26	PFAM
Pfam:NCD3G	508	562	1.1e-18	PFAM
Pfam:7tm_3	595	831	3.7e-54	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.5%

Validation Efficiency

100% (60/60)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8b	G	A	11: 109,981,725	Q17*	probably null	Het
Acap2	T	A	16: 31,119,427	N293I	probably damaging	Het
Adgrg4	G	A	X: 56,918,749	V1893I	possibly damaging	Het
Aff1	T	C	5: 103,818,988		probably null	Het
Ap1b1	A	G	11: 5,031,637	D515G	probably damaging	Het
Brk1	T	C	6: 113,615,844	Y63H	possibly damaging	Het
Cand1	T	C	10: 119,211,845	D580G	probably damaging	Het
Casp4	A	G	9: 5,328,451	D311G	probably benign	Het
Crygs	C	T	16: 22,805,551	G102D	possibly damaging	Het
Ctnnd2	G	T	15: 30,972,827	V1033F	probably damaging	Het
Dhx29	G	T	13: 112,927,949	A53S	probably benign	Het
Dis3	T	G	14: 99,095,316	I227L	probably benign	Het
Efhc2	T	C	X: 17,230,550	N186S	possibly damaging	Het
Efl1	T	C	7: 82,750,816	V592A	probably damaging	Het
Elovl7	A	T	13: 108,282,506	Q224L	possibly damaging	Het
Fcgr3	T	A	1: 171,054,075	K160N	probably benign	Het
Flg	A	G	3: 93,279,862	Y207C	probably benign	Het
Fmn2	A	G	1: 174,581,955	T585A	unknown	Het
Gm7135	T	C	1: 97,469,895		noncoding transcript	Het
Gm8104	G	T	14: 43,101,634	D94Y	probably damaging	Het
Hjurp	G	C	1: 88,277,215		probably benign	Het
Ino80b	G	C	6: 83,122,333	P178R	probably damaging	Het
Kbtbd4	T	C	2: 90,909,755	F495L	probably damaging	Het
Lingo2	T	C	4: 35,709,810	I57V	probably benign	Het
Me2	T	C	18: 73,791,085	K352R	probably benign	Het
Mthfsd	A	G	8: 121,105,626	V133A	probably damaging	Het
Nav2	T	A	7: 49,572,298		probably null	Het
Nav2	T	A	7: 49,597,231	I2168N	probably damaging	Het
Nlrp10	T	A	7: 108,924,341	D644V	possibly damaging	Het
Olfr1472	T	C	19: 13,454,471	I15M	probably benign	Het
Olfr148	A	G	9: 39,613,957	Y130C	possibly damaging	Het
Olfr15	T	C	16: 3,839,570	L199P	probably damaging	Het
Oplah	C	T	15: 76,302,710	R635H	probably damaging	Het
Peli1	A	G	11: 21,147,115		probably null	Het
Pfkfb1	A	T	X: 150,622,188	D208V	possibly damaging	Het
Pld5	T	G	1: 175,993,875	T242P	probably damaging	Het
Rbm5	A	G	9: 107,750,483	S420P	probably benign	Het
Rhag	A	T	17: 40,831,653	I250F	probably damaging	Het
Rnase4	A	G	14: 51,105,005	K62R	probably benign	Het
Scaf4	C	T	16: 90,260,215	V83I	unknown	Het
Slc24a2	A	G	4: 87,227,205	V204A	probably damaging	Het
Slc5a8	G	T	10: 88,911,413	L409F	probably damaging	Het
Spns3	A	T	11: 72,538,361	V199E	probably damaging	Het
Sspo	A	G	6: 48,478,293	T3030A	probably benign	Het
Sstr2	A	C	11: 113,624,656	T134P	probably damaging	Het
Stk39	G	T	2: 68,220,920	T527K	probably benign	Het
Sult2a1	T	A	7: 13,801,547	T194S	probably benign	Het
Tamm41	AGGG	AGG	6: 115,012,359		probably benign	Het
Trav7-3	A	G	14: 53,443,746	T82A	probably benign	Het
Umodl1	G	A	17: 30,959,367	V106I	probably damaging	Het
Vmn2r92	G	A	17: 18,184,261	V556M	possibly damaging	Het
Zfp292	T	C	4: 34,806,079	I2322V	probably benign	Het
Zfp644	T	C	5: 106,618,276	E93G	probably damaging	Het
Zfp81	C	T	17: 33,334,916	C308Y	probably damaging	Het

Other mutations in Vmn2r85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Vmn2r85	APN	10	130418821	missense	probably benign	0.22
IGL01298:Vmn2r85	APN	10	130418821	missense	probably benign	0.22
IGL01361:Vmn2r85	APN	10	130418821	missense	probably benign	0.22
IGL02185:Vmn2r85	APN	10	130418692	missense	probably benign	0.13
IGL02505:Vmn2r85	APN	10	130425580	missense	probably damaging	1.00
IGL02607:Vmn2r85	APN	10	130426421	missense	possibly damaging	0.89
IGL02755:Vmn2r85	APN	10	130425512	missense	probably damaging	0.98
IGL03188:Vmn2r85	APN	10	130418743	missense	probably benign	0.16
IGL03366:Vmn2r85	APN	10	130426459	missense	probably benign	0.00
IGL03397:Vmn2r85	APN	10	130425394	missense	probably damaging	1.00
PIT4445001:Vmn2r85	UTSW	10	130425703	missense	probably benign	0.00
R0066:Vmn2r85	UTSW	10	130425901	missense	probably damaging	1.00
R0128:Vmn2r85	UTSW	10	130419185	splice site	probably benign
R0130:Vmn2r85	UTSW	10	130419185	splice site	probably benign
R0503:Vmn2r85	UTSW	10	130422740	missense	probably damaging	1.00
R0827:Vmn2r85	UTSW	10	130429518	missense	possibly damaging	0.89
R1432:Vmn2r85	UTSW	10	130425286	missense	possibly damaging	0.74
R1521:Vmn2r85	UTSW	10	130425919	missense	probably damaging	0.99
R2029:Vmn2r85	UTSW	10	130425574	nonsense	probably null
R2034:Vmn2r85	UTSW	10	130426373	splice site	probably benign
R2852:Vmn2r85	UTSW	10	130419166	missense	probably benign	0.03
R2853:Vmn2r85	UTSW	10	130419166	missense	probably benign	0.03
R3084:Vmn2r85	UTSW	10	130425212	missense	probably benign	0.00
R3085:Vmn2r85	UTSW	10	130425212	missense	probably benign	0.00
R3430:Vmn2r85	UTSW	10	130418889	missense	probably damaging	0.97
R3694:Vmn2r85	UTSW	10	130418302	missense	probably damaging	0.99
R3932:Vmn2r85	UTSW	10	130418467	missense	probably damaging	1.00
R4628:Vmn2r85	UTSW	10	130425366	missense	probably benign	0.00
R4814:Vmn2r85	UTSW	10	130418698	missense	probably benign	0.12
R4948:Vmn2r85	UTSW	10	130419121	missense	probably damaging	1.00
R4951:Vmn2r85	UTSW	10	130425244	missense	probably damaging	1.00
R4959:Vmn2r85	UTSW	10	130421433	missense	probably damaging	1.00
R5336:Vmn2r85	UTSW	10	130422705	missense	possibly damaging	0.63
R5643:Vmn2r85	UTSW	10	130426474	missense	probably damaging	1.00
R6061:Vmn2r85	UTSW	10	130425662	missense	probably benign	0.09
R6115:Vmn2r85	UTSW	10	130422803	missense	probably damaging	1.00
R6190:Vmn2r85	UTSW	10	130425461	missense	possibly damaging	0.88
R6518:Vmn2r85	UTSW	10	130429412	missense	probably benign	0.00
R6533:Vmn2r85	UTSW	10	130426660	missense	probably benign	0.00
R6610:Vmn2r85	UTSW	10	130425969	missense	probably damaging	0.97
R6809:Vmn2r85	UTSW	10	130425926	missense	probably benign
R6962:Vmn2r85	UTSW	10	130425583	missense	probably damaging	0.99
R7075:Vmn2r85	UTSW	10	130422688	missense	probably benign	0.06
R7104:Vmn2r85	UTSW	10	130426507	missense	probably benign
R7424:Vmn2r85	UTSW	10	130418980	missense	probably damaging	1.00
R7516:Vmn2r85	UTSW	10	130418983	missense	probably damaging	1.00
R7537:Vmn2r85	UTSW	10	130422866	missense	probably benign	0.01
R7768:Vmn2r85	UTSW	10	130418693	missense	probably damaging	1.00
R7810:Vmn2r85	UTSW	10	130425212	missense	probably benign	0.00
R8078:Vmn2r85	UTSW	10	130429495	nonsense	probably null
R8115:Vmn2r85	UTSW	10	130425951	missense	probably benign	0.06
R8262:Vmn2r85	UTSW	10	130418869	missense	probably damaging	0.98
R8395:Vmn2r85	UTSW	10	130425928	missense	probably damaging	0.99
R8409:Vmn2r85	UTSW	10	130425388	missense	probably benign	0.16
R8547:Vmn2r85	UTSW	10	130425442	missense	probably damaging	1.00
R8875:Vmn2r85	UTSW	10	130418302	missense	probably damaging	0.99
R9035:Vmn2r85	UTSW	10	130425610	missense	probably benign
R9040:Vmn2r85	UTSW	10	130418442	missense	probably damaging	1.00
R9115:Vmn2r85	UTSW	10	130418284	missense	probably benign	0.00
R9182:Vmn2r85	UTSW	10	130429481	missense	probably benign	0.00
R9245:Vmn2r85	UTSW	10	130419164	missense	possibly damaging	0.92
R9245:Vmn2r85	UTSW	10	130425665	missense	probably damaging	1.00
R9405:Vmn2r85	UTSW	10	130425346	missense	probably damaging	0.99
R9502:Vmn2r85	UTSW	10	130425518	missense	probably damaging	0.99
R9520:Vmn2r85	UTSW	10	130419124	missense	probably benign
R9653:Vmn2r85	UTSW	10	130425825	missense	probably damaging	0.99
Z1176:Vmn2r85	UTSW	10	130425844	missense	probably damaging	0.99
Z1177:Vmn2r85	UTSW	10	130418907	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAAAGCCACAGTGAAGCTCC -3'
(R):5'- TGAAGGCCAATAACTGCATTCTC -3'

Sequencing Primer
(F):5'- ACAGTGAAGCTCCCCAGAG -3'
(R):5'- GGCCAATAACTGCATTCTCAGCTAC -3'

Posted On

2015-06-10