Incidental Mutation 'R4177:Mrgpra9'
| ID |
319557 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Mrgpra9
|
| Ensembl Gene |
ENSMUSG00000074111 |
| Gene Name |
MAS-related GPR, member A9 |
| Synonyms |
MrgA9, EG668725 |
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.049)
|
| Stock # |
R4177 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
7 |
| Chromosomal Location |
46884667-46902627 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 46885302 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Tyrosine to Histidine
at position 122
(Y122H)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000136396
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000098436]
[ENSMUST00000179005]
|
| AlphaFold |
A0A140T8U8 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000098436
AA Change: Y121H
PolyPhen 2
Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000096035
Gene: ENSMUSG00000074111
AA Change: Y121H
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
37 |
48 |
N/A |
INTRINSIC |
|
Pfam:7tm_1
|
56 |
225 |
1.5e-11 |
PFAM |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000179005
AA Change: Y122H
PolyPhen 2
Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000136396
Gene: ENSMUSG00000074111
AA Change: Y122H
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_1
|
12 |
178 |
3.4e-9 |
PFAM |
|
| Meta Mutation Damage Score |
0.6467 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.3%
- 20x: 95.2%
|
| Validation Efficiency |
100% (46/46) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 37 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Aicda |
A |
T |
6: 122,538,043 (GRCm39) |
D67V |
probably benign |
Het |
| Arhgap32 |
A |
G |
9: 32,158,510 (GRCm39) |
R182G |
probably null |
Het |
| Bbx |
T |
C |
16: 50,045,221 (GRCm39) |
K447E |
probably damaging |
Het |
| Bscl2 |
A |
G |
19: 8,817,120 (GRCm39) |
D16G |
possibly damaging |
Het |
| Celsr2 |
A |
G |
3: 108,321,294 (GRCm39) |
V506A |
probably damaging |
Het |
| Cenpf |
T |
A |
1: 189,400,816 (GRCm39) |
Q441L |
possibly damaging |
Het |
| Crebbp |
A |
T |
16: 3,937,663 (GRCm39) |
M700K |
possibly damaging |
Het |
| Dhcr7 |
A |
G |
7: 143,394,910 (GRCm39) |
Y142C |
probably damaging |
Het |
| Dnajc2 |
A |
G |
5: 21,962,394 (GRCm39) |
M602T |
probably benign |
Het |
| Dysf |
C |
T |
6: 84,044,013 (GRCm39) |
R254* |
probably null |
Het |
| Ednra |
A |
C |
8: 78,401,677 (GRCm39) |
I204M |
possibly damaging |
Het |
| Fabp2 |
A |
G |
3: 122,690,547 (GRCm39) |
T77A |
possibly damaging |
Het |
| Gm10322 |
C |
A |
10: 59,452,052 (GRCm39) |
N56K |
probably benign |
Het |
| Gnb1 |
T |
C |
4: 155,625,113 (GRCm39) |
|
probably benign |
Het |
| Gpc2 |
T |
C |
5: 138,275,621 (GRCm39) |
|
probably benign |
Het |
| Kmt2a |
T |
C |
9: 44,732,280 (GRCm39) |
|
probably benign |
Het |
| Lypla2 |
T |
C |
4: 135,696,403 (GRCm39) |
|
probably benign |
Het |
| Maf |
A |
T |
8: 116,433,210 (GRCm39) |
Y131* |
probably null |
Het |
| Mfsd4a |
T |
A |
1: 131,968,295 (GRCm39) |
H335L |
probably damaging |
Het |
| Myo10 |
A |
T |
15: 25,734,137 (GRCm39) |
Q342L |
possibly damaging |
Het |
| N4bp2 |
A |
G |
5: 65,955,513 (GRCm39) |
|
probably null |
Het |
| Or5t15 |
A |
T |
2: 86,681,745 (GRCm39) |
M99K |
possibly damaging |
Het |
| Pomt1 |
A |
G |
2: 32,138,689 (GRCm39) |
N435S |
probably damaging |
Het |
| Pram1 |
A |
G |
17: 33,860,203 (GRCm39) |
I257V |
probably benign |
Het |
| Rab9 |
G |
T |
X: 165,241,296 (GRCm39) |
S5* |
probably null |
Het |
| Ralgapa2 |
A |
T |
2: 146,327,083 (GRCm39) |
Y59N |
probably damaging |
Het |
| Rasgrf2 |
C |
T |
13: 92,038,717 (GRCm39) |
G1043D |
probably damaging |
Het |
| Rps6kc1 |
G |
A |
1: 190,532,616 (GRCm39) |
T462M |
possibly damaging |
Het |
| Selenoo |
C |
T |
15: 88,983,662 (GRCm39) |
|
probably benign |
Het |
| Sptb |
G |
C |
12: 76,659,953 (GRCm39) |
D982E |
probably benign |
Het |
| Tab2 |
A |
G |
10: 7,795,123 (GRCm39) |
V453A |
probably damaging |
Het |
| Ubald1 |
A |
G |
16: 4,693,745 (GRCm39) |
|
probably benign |
Het |
| Ube2d1 |
T |
C |
10: 71,094,033 (GRCm39) |
K101R |
probably damaging |
Het |
| Unc5cl |
A |
G |
17: 48,769,298 (GRCm39) |
T261A |
probably benign |
Het |
| Zcwpw1 |
G |
T |
5: 137,798,395 (GRCm39) |
K197N |
probably damaging |
Het |
| Zfp268 |
T |
C |
4: 145,347,225 (GRCm39) |
L43S |
probably damaging |
Het |
| Zfyve19 |
A |
G |
2: 119,046,693 (GRCm39) |
T296A |
possibly damaging |
Het |
|
| Other mutations in Mrgpra9 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00095:Mrgpra9
|
APN |
7 |
46,884,839 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
IGL00575:Mrgpra9
|
APN |
7 |
46,885,053 (GRCm39) |
missense |
possibly damaging |
0.76 |
|
IGL01649:Mrgpra9
|
APN |
7 |
46,884,900 (GRCm39) |
missense |
probably benign |
0.22 |
|
IGL03207:Mrgpra9
|
APN |
7 |
46,885,385 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R0388:Mrgpra9
|
UTSW |
7 |
46,902,542 (GRCm39) |
start codon destroyed |
probably null |
0.08 |
|
R0972:Mrgpra9
|
UTSW |
7 |
46,885,203 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1270:Mrgpra9
|
UTSW |
7 |
46,902,531 (GRCm39) |
critical splice donor site |
probably null |
|
|
R1381:Mrgpra9
|
UTSW |
7 |
46,885,050 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R1403:Mrgpra9
|
UTSW |
7 |
46,885,386 (GRCm39) |
missense |
probably benign |
0.24 |
|
R1403:Mrgpra9
|
UTSW |
7 |
46,885,386 (GRCm39) |
missense |
probably benign |
0.24 |
|
R1448:Mrgpra9
|
UTSW |
7 |
46,885,561 (GRCm39) |
missense |
probably benign |
0.02 |
|
R2045:Mrgpra9
|
UTSW |
7 |
46,885,583 (GRCm39) |
missense |
probably benign |
|
|
R2144:Mrgpra9
|
UTSW |
7 |
46,885,211 (GRCm39) |
missense |
probably benign |
0.31 |
|
R2187:Mrgpra9
|
UTSW |
7 |
46,884,797 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2507:Mrgpra9
|
UTSW |
7 |
46,885,242 (GRCm39) |
missense |
possibly damaging |
0.63 |
|
R2913:Mrgpra9
|
UTSW |
7 |
46,884,828 (GRCm39) |
missense |
probably benign |
|
|
R3810:Mrgpra9
|
UTSW |
7 |
46,885,527 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4521:Mrgpra9
|
UTSW |
7 |
46,884,938 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4781:Mrgpra9
|
UTSW |
7 |
46,884,795 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R4926:Mrgpra9
|
UTSW |
7 |
46,884,759 (GRCm39) |
missense |
possibly damaging |
0.62 |
|
R6469:Mrgpra9
|
UTSW |
7 |
46,884,854 (GRCm39) |
missense |
probably benign |
0.02 |
|
R6505:Mrgpra9
|
UTSW |
7 |
46,884,884 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6724:Mrgpra9
|
UTSW |
7 |
46,884,786 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7398:Mrgpra9
|
UTSW |
7 |
46,885,385 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R8737:Mrgpra9
|
UTSW |
7 |
46,885,624 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8824:Mrgpra9
|
UTSW |
7 |
46,885,041 (GRCm39) |
missense |
probably benign |
0.06 |
|
R8881:Mrgpra9
|
UTSW |
7 |
46,885,242 (GRCm39) |
missense |
possibly damaging |
0.63 |
|
R9320:Mrgpra9
|
UTSW |
7 |
46,885,392 (GRCm39) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- ATGCCATACACCCATAGTCATTTAC -3'
(R):5'- AAACGCCATTGTGTTCTGGC -3'
Sequencing Primer
(F):5'- TGGTATCTAAGAAACCGCAGAAATAC -3'
(R):5'- CCATTGTGTTCTGGCTCCTGG -3'
|
| Posted On |
2015-06-10 |
Incidental Mutation