Mutagenetix > Incidental Mutation

Incidental Mutation 'R0395:Tle3'

31957

Institutional Source

Beutler Lab

Gene Symbol

Tle3

Ensembl Gene

ENSMUSG00000032280

Gene Name

transducin-like enhancer of split 3

Synonyms

2610103N05Rik, ESG, Grg3a, Grg3b

MMRRC Submission

038601-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0395 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

61279648-61325779 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 61317353 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 334 (M334K)

Ref Sequence

ENSEMBL: ENSMUSP00000124150 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034820] [ENSMUST00000159050] [ENSMUST00000159386] [ENSMUST00000159630] [ENSMUST00000160724] [ENSMUST00000160882] [ENSMUST00000178113] [ENSMUST00000161207] [ENSMUST00000161689] [ENSMUST00000162973] [ENSMUST00000162127] [ENSMUST00000162583] [ENSMUST00000161993]

AlphaFold

Q08122

Predicted Effect

possibly damaging
Transcript: ENSMUST00000034820
AA Change: M396K

PolyPhen 2 Score 0.464 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000034820
Gene: ENSMUSG00000032280
AA Change: M396K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	136	4.3e-77	PFAM
low complexity region	161	179	N/A	INTRINSIC
low complexity region	195	207	N/A	INTRINSIC
low complexity region	283	302	N/A	INTRINSIC
WD40	468	505	2.96e-2	SMART
WD40	511	552	4.48e-2	SMART
WD40	557	596	2.84e-4	SMART
WD40	599	638	7.55e-9	SMART
WD40	641	679	3.07e1	SMART
WD40	681	720	4.18e-2	SMART
WD40	721	761	1.79e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000159050
AA Change: M399K

PolyPhen 2 Score 0.503 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000125032
Gene: ENSMUSG00000032280
AA Change: M399K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	136	2.1e-77	PFAM
low complexity region	161	179	N/A	INTRINSIC
low complexity region	195	207	N/A	INTRINSIC
low complexity region	283	302	N/A	INTRINSIC
WD40	471	508	2.96e-2	SMART
WD40	514	555	4.48e-2	SMART
WD40	560	599	2.84e-4	SMART
WD40	602	641	7.55e-9	SMART
WD40	644	682	3.07e1	SMART
WD40	684	723	4.18e-2	SMART
WD40	724	764	1.79e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159140

Predicted Effect

possibly damaging
Transcript: ENSMUST00000159386
AA Change: M387K

PolyPhen 2 Score 0.464 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000125049
Gene: ENSMUSG00000032280
AA Change: M387K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	136	2.1e-77	PFAM
low complexity region	161	179	N/A	INTRINSIC
low complexity region	195	207	N/A	INTRINSIC
low complexity region	283	302	N/A	INTRINSIC
WD40	464	501	2.96e-2	SMART
WD40	507	548	4.48e-2	SMART
WD40	553	592	2.84e-4	SMART
WD40	595	634	7.55e-9	SMART
WD40	637	675	3.07e1	SMART
WD40	677	716	4.18e-2	SMART
WD40	717	757	1.79e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000159630
AA Change: M339K

PolyPhen 2 Score 0.777 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000123723
Gene: ENSMUSG00000032280
AA Change: M339K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	79	1.7e-43	PFAM
low complexity region	104	122	N/A	INTRINSIC
low complexity region	138	150	N/A	INTRINSIC
low complexity region	226	245	N/A	INTRINSIC
WD40	416	453	2.96e-2	SMART
WD40	459	500	4.48e-2	SMART
WD40	505	544	2.84e-4	SMART
WD40	547	586	7.55e-9	SMART
WD40	589	627	3.07e1	SMART
WD40	629	668	4.18e-2	SMART
WD40	669	709	1.79e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000160724

SMART Domains

Protein: ENSMUSP00000124055
Gene: ENSMUSG00000032280

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	145	1.9e-77	PFAM
low complexity region	170	188	N/A	INTRINSIC
low complexity region	204	216	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160882
AA Change: M409K

PolyPhen 2 Score 0.806 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000124131
Gene: ENSMUSG00000032280
AA Change: M409K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	146	4.8e-76	PFAM
low complexity region	171	189	N/A	INTRINSIC
low complexity region	205	217	N/A	INTRINSIC
low complexity region	293	312	N/A	INTRINSIC
WD40	486	523	2.96e-2	SMART
WD40	529	570	4.48e-2	SMART
WD40	575	614	2.84e-4	SMART
WD40	617	656	7.55e-9	SMART
WD40	659	697	3.07e1	SMART
WD40	699	738	4.18e-2	SMART
WD40	739	779	1.79e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000178113
AA Change: M410K

PolyPhen 2 Score 0.263 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000136010
Gene: ENSMUSG00000032280
AA Change: M410K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	143	6e-73	PFAM
low complexity region	171	189	N/A	INTRINSIC
low complexity region	204	218	N/A	INTRINSIC
low complexity region	294	313	N/A	INTRINSIC
WD40	487	524	2.96e-2	SMART
WD40	530	571	4.48e-2	SMART
WD40	576	615	2.84e-4	SMART
WD40	618	657	7.55e-9	SMART
WD40	660	698	3.07e1	SMART
WD40	700	739	4.18e-2	SMART
WD40	740	780	1.79e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000161207
AA Change: M389K

PolyPhen 2 Score 0.457 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000124557
Gene: ENSMUSG00000032280
AA Change: M389K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	129	1e-73	PFAM
low complexity region	154	172	N/A	INTRINSIC
low complexity region	188	200	N/A	INTRINSIC
low complexity region	276	295	N/A	INTRINSIC
WD40	466	503	2.96e-2	SMART
WD40	509	550	4.48e-2	SMART
WD40	555	594	2.84e-4	SMART
WD40	597	636	7.55e-9	SMART
WD40	639	677	3.07e1	SMART
WD40	679	718	4.18e-2	SMART
WD40	719	759	1.79e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000161689
AA Change: M343K

PolyPhen 2 Score 0.777 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000125011
Gene: ENSMUSG00000032280
AA Change: M343K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	80	4.8e-44	PFAM
low complexity region	105	123	N/A	INTRINSIC
low complexity region	139	151	N/A	INTRINSIC
low complexity region	227	246	N/A	INTRINSIC
WD40	420	457	2.96e-2	SMART
WD40	463	504	4.48e-2	SMART
WD40	509	548	2.84e-4	SMART
WD40	551	590	7.55e-9	SMART
WD40	593	631	3.07e1	SMART
WD40	633	672	4.18e-2	SMART
WD40	673	713	1.79e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000162973
AA Change: M399K

PolyPhen 2 Score 0.464 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000124173
Gene: ENSMUSG00000032280
AA Change: M399K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	136	3.9e-77	PFAM
low complexity region	161	179	N/A	INTRINSIC
low complexity region	195	207	N/A	INTRINSIC
low complexity region	283	302	N/A	INTRINSIC
WD40	476	513	2.96e-2	SMART
WD40	519	560	4.48e-2	SMART
WD40	565	604	2.84e-4	SMART
WD40	607	646	7.55e-9	SMART
WD40	649	687	3.07e1	SMART
WD40	689	728	4.18e-2	SMART
WD40	729	769	1.79e-1	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000162127
AA Change: M334K

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000124150
Gene: ENSMUSG00000032280
AA Change: M334K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	83	1.2e-37	PFAM
low complexity region	108	126	N/A	INTRINSIC
low complexity region	142	154	N/A	INTRINSIC
low complexity region	230	249	N/A	INTRINSIC
WD40	406	443	2.96e-2	SMART
WD40	449	490	4.48e-2	SMART
WD40	495	534	2.84e-4	SMART
WD40	537	576	7.55e-9	SMART
WD40	579	617	3.07e1	SMART
WD40	619	658	4.18e-2	SMART
WD40	659	699	1.79e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000162583
AA Change: M396K

PolyPhen 2 Score 0.599 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000124977
Gene: ENSMUSG00000032280
AA Change: M396K

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	136	3.9e-77	PFAM
low complexity region	161	179	N/A	INTRINSIC
low complexity region	195	207	N/A	INTRINSIC
low complexity region	283	302	N/A	INTRINSIC
WD40	473	510	2.96e-2	SMART
WD40	516	557	4.48e-2	SMART
WD40	562	601	2.84e-4	SMART
WD40	604	643	7.55e-9	SMART
WD40	646	684	3.07e1	SMART
WD40	686	725	4.18e-2	SMART
WD40	726	766	1.79e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162962

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162281

Predicted Effect

probably benign
Transcript: ENSMUST00000161993

SMART Domains

Protein: ENSMUSP00000124432
Gene: ENSMUSG00000032280

Domain	Start	End	E-Value	Type
Pfam:TLE_N	1	135	2e-77	PFAM
low complexity region	160	178	N/A	INTRINSIC
low complexity region	194	206	N/A	INTRINSIC
low complexity region	282	301	N/A	INTRINSIC

Meta Mutation Damage Score

0.2683

Coding Region Coverage

1x: 99.1%
3x: 98.1%
10x: 95.9%
20x: 91.4%

Validation Efficiency

98% (103/105)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]
PHENOTYPE: Mice homzoygous for a gene trap allele exhibit embryonic lethality. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921517D22Rik	T	C	13: 59,837,470 (GRCm39)	K205R	possibly damaging	Het
9630041A04Rik	A	T	9: 101,819,934 (GRCm39)	N118I	probably damaging	Het
AAdacl4fm3	A	G	4: 144,429,765 (GRCm39)	V408A	probably benign	Het
Acsm2	G	A	7: 119,174,969 (GRCm39)	D245N	probably damaging	Het
Adgrv1	A	T	13: 81,534,072 (GRCm39)	H5836Q	probably benign	Het
Ahcyl2	T	C	6: 29,886,167 (GRCm39)	V391A	probably damaging	Het
Alcam	A	T	16: 52,130,227 (GRCm39)	M41K	probably benign	Het
Aldh3b3	A	C	19: 4,016,472 (GRCm39)	E363D	probably benign	Het
Alk	A	G	17: 72,910,526 (GRCm39)	V60A	probably damaging	Het
Als2cl	G	A	9: 110,727,152 (GRCm39)	R906H	probably damaging	Het
Ap5z1	T	C	5: 142,456,317 (GRCm39)		probably benign	Het
Apba2	T	A	7: 64,393,156 (GRCm39)	I547N	probably benign	Het
Apol10b	A	T	15: 77,469,840 (GRCm39)	D112E	probably damaging	Het
Ash1l	C	A	3: 88,965,896 (GRCm39)	R2433S	probably damaging	Het
Cachd1	T	C	4: 100,810,402 (GRCm39)	F335L	probably damaging	Het
Cbfa2t3	G	T	8: 123,365,690 (GRCm39)	Q181K	probably benign	Het
Cct6b	A	G	11: 82,630,506 (GRCm39)	M265T	probably benign	Het
Cd151	G	A	7: 141,050,304 (GRCm39)	V180I	probably damaging	Het
Ces1h	T	A	8: 94,083,706 (GRCm39)	N412I	unknown	Het
Chmp7	T	C	14: 69,969,905 (GRCm39)	T12A	probably benign	Het
Clasp1	A	G	1: 118,467,061 (GRCm39)	T534A	possibly damaging	Het
Cldn15	T	A	5: 136,997,052 (GRCm39)	V31E	possibly damaging	Het
Col16a1	G	A	4: 129,966,902 (GRCm39)	G583D	probably damaging	Het
Csmd1	T	C	8: 16,396,652 (GRCm39)	N426S	probably damaging	Het
Dapp1	C	T	3: 137,641,398 (GRCm39)	C199Y	possibly damaging	Het
Dchs1	A	G	7: 105,407,745 (GRCm39)	L2029P	probably damaging	Het
Dmc1	A	G	15: 79,472,973 (GRCm39)	F158S	probably damaging	Het
Dst	C	T	1: 34,228,200 (GRCm39)	P1606L	probably damaging	Het
Dthd1	T	A	5: 62,971,676 (GRCm39)	N166K	possibly damaging	Het
Enam	A	T	5: 88,649,367 (GRCm39)	Y292F	probably damaging	Het
Esrrg	A	T	1: 187,930,832 (GRCm39)	I285F	probably damaging	Het
Fam50b	A	G	13: 34,931,220 (GRCm39)	D232G	probably damaging	Het
Fam91a1	T	A	15: 58,326,641 (GRCm39)	S792T	probably benign	Het
Fbxw22	A	C	9: 109,210,753 (GRCm39)	C419W	probably damaging	Het
Flt4	G	A	11: 49,521,170 (GRCm39)	S393N	probably benign	Het
Fras1	A	T	5: 96,917,512 (GRCm39)	T3511S	possibly damaging	Het
Frat2	A	C	19: 41,836,263 (GRCm39)	S30A	probably damaging	Het
Glp1r	T	A	17: 31,155,312 (GRCm39)	M433K	probably benign	Het
Gm10300	G	A	4: 131,802,299 (GRCm39)		probably benign	Het
Gpatch4	A	G	3: 87,961,661 (GRCm39)		probably benign	Het
Gpr22	A	T	12: 31,759,461 (GRCm39)	S220R	possibly damaging	Het
Grn	T	C	11: 102,327,049 (GRCm39)	V549A	probably benign	Het
Gtf3c5	T	C	2: 28,467,930 (GRCm39)	D177G	probably damaging	Het
Htr1b	A	T	9: 81,513,704 (GRCm39)	M301K	probably benign	Het
Ifi207	A	T	1: 173,557,431 (GRCm39)	S436T	possibly damaging	Het
Ifnb1	A	T	4: 88,440,766 (GRCm39)	N82K	possibly damaging	Het
Ina	T	G	19: 47,010,358 (GRCm39)	N384K	probably damaging	Het
Kirrel2	T	C	7: 30,149,883 (GRCm39)	N541D	possibly damaging	Het
Lrp2	A	T	2: 69,263,421 (GRCm39)	I4377N	possibly damaging	Het
Lrrc37a	A	G	11: 103,355,221 (GRCm39)	V2532A	unknown	Het
Mast4	T	C	13: 102,871,781 (GRCm39)	E2529G	probably damaging	Het
Myh6	T	C	14: 55,183,777 (GRCm39)	H1719R	possibly damaging	Het
Myo5a	A	T	9: 75,101,259 (GRCm39)	H150L	probably benign	Het
Naglu	C	T	11: 100,964,933 (GRCm39)		probably benign	Het
Nags	G	A	11: 102,036,530 (GRCm39)	A40T	unknown	Het
Nav1	G	T	1: 135,460,359 (GRCm39)	Y321*	probably null	Het
Nav1	A	T	1: 135,460,361 (GRCm39)	Y321N	probably damaging	Het
Neu3	C	T	7: 99,462,985 (GRCm39)	S246N	probably benign	Het
Npy5r	C	T	8: 67,134,625 (GRCm39)	G56D	probably benign	Het
Nrxn1	A	G	17: 91,395,742 (GRCm39)	V138A	possibly damaging	Het
Nuggc	C	T	14: 65,850,921 (GRCm39)	Q264*	probably null	Het
Ogfod1	G	A	8: 94,790,156 (GRCm39)		probably null	Het
Or1o11	T	A	17: 37,756,757 (GRCm39)	F115Y	probably damaging	Het
Or2t45	A	T	11: 58,669,195 (GRCm39)	M81L	probably benign	Het
Or5b101	A	G	19: 13,005,663 (GRCm39)	F10S	probably damaging	Het
Or5p66	A	G	7: 107,885,478 (GRCm39)	V285A	probably benign	Het
Per1	T	A	11: 68,993,103 (GRCm39)	I340N	probably damaging	Het
Pkhd1	C	T	1: 20,451,771 (GRCm39)	A2175T	probably benign	Het
Pogk	A	G	1: 166,231,171 (GRCm39)	V52A	probably damaging	Het
Pou2af3	C	T	9: 51,191,834 (GRCm39)		probably benign	Het
Ppib	A	T	9: 65,973,601 (GRCm39)	T185S	possibly damaging	Het
Ptar1	G	T	19: 23,697,563 (GRCm39)	M358I	probably damaging	Het
Qser1	A	C	2: 104,593,226 (GRCm39)	I1597S	probably damaging	Het
Ranbp17	T	C	11: 33,424,896 (GRCm39)	I487V	probably benign	Het
Repin1	C	A	6: 48,574,459 (GRCm39)	R460S	probably damaging	Het
Sfmbt1	T	C	14: 30,509,574 (GRCm39)		probably benign	Het
Sh3rf1	C	T	8: 61,846,696 (GRCm39)		probably benign	Het
Shroom3	C	T	5: 92,928,762 (GRCm39)	R106C	probably damaging	Het
Siglecf	T	A	7: 43,005,399 (GRCm39)	V453D	probably damaging	Het
Slc2a9	A	G	5: 38,610,512 (GRCm39)	S96P	probably damaging	Het
Slc5a2	A	G	7: 127,866,654 (GRCm39)	Y124C	probably damaging	Het
Slf1	A	G	13: 77,254,088 (GRCm39)		probably benign	Het
Smad1	T	G	8: 80,076,411 (GRCm39)	K269T	probably benign	Het
Srp54b	G	A	12: 55,296,884 (GRCm39)	R194H	probably damaging	Het
St8sia6	T	A	2: 13,670,247 (GRCm39)	S238C	probably damaging	Het
Stat3	A	T	11: 100,780,763 (GRCm39)		probably benign	Het
Tafa2	A	T	10: 123,429,497 (GRCm39)	H37L	probably benign	Het
Tas1r2	T	A	4: 139,382,665 (GRCm39)	M101K	possibly damaging	Het
Tesc	A	G	5: 118,191,647 (GRCm39)		probably null	Het
Tmem151a	A	T	19: 5,132,261 (GRCm39)	V315E	probably damaging	Het
Tmprss2	T	C	16: 97,368,245 (GRCm39)	D480G	probably damaging	Het
Trmt1	C	A	8: 85,423,741 (GRCm39)		probably null	Het
Tsr3	T	C	17: 25,461,198 (GRCm39)		probably null	Het
Ube2u	A	G	4: 100,338,845 (GRCm39)	K37E	probably benign	Het
Usp16	T	A	16: 87,272,334 (GRCm39)	D382E	probably damaging	Het
Usp9y	A	T	Y: 1,340,053 (GRCm39)	F1442Y	probably damaging	Het
Utp20	A	T	10: 88,654,457 (GRCm39)	M210K	probably damaging	Het
Utp25	T	C	1: 192,805,984 (GRCm39)	E187G	possibly damaging	Het
V1ra8	C	T	6: 90,179,991 (GRCm39)	L65F	possibly damaging	Het
Vmn2r10	T	C	5: 109,149,859 (GRCm39)	N395S	probably damaging	Het
Vmn2r100	C	A	17: 19,742,382 (GRCm39)	P252Q	possibly damaging	Het
Zfp217	C	T	2: 169,957,382 (GRCm39)	A539T	probably benign	Het
Zfp330	G	A	8: 83,491,511 (GRCm39)	Q221*	probably null	Het

Other mutations in Tle3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00494:Tle3	APN	9	61,316,039 (GRCm39)	splice site	probably benign
IGL00671:Tle3	APN	9	61,319,652 (GRCm39)	missense	probably damaging	1.00
IGL01583:Tle3	APN	9	61,317,307 (GRCm39)	missense	probably benign	0.00
IGL01684:Tle3	APN	9	61,310,728 (GRCm39)	intron	probably benign
IGL02109:Tle3	APN	9	61,320,332 (GRCm39)	missense	probably damaging	1.00
IGL02386:Tle3	APN	9	61,301,941 (GRCm39)	missense	possibly damaging	0.88
IGL02517:Tle3	APN	9	61,322,063 (GRCm39)	missense	probably damaging	1.00
IGL02930:Tle3	APN	9	61,301,981 (GRCm39)	missense	possibly damaging	0.52
IGL03103:Tle3	APN	9	61,300,524 (GRCm39)	missense	possibly damaging	0.46
R0391:Tle3	UTSW	9	61,323,943 (GRCm39)	missense	probably damaging	1.00
R0621:Tle3	UTSW	9	61,317,387 (GRCm39)	nonsense	probably null
R1836:Tle3	UTSW	9	61,321,305 (GRCm39)	missense	probably damaging	1.00
R1921:Tle3	UTSW	9	61,318,622 (GRCm39)	critical splice donor site	probably null
R1978:Tle3	UTSW	9	61,301,915 (GRCm39)	missense	probably damaging	1.00
R3434:Tle3	UTSW	9	61,321,376 (GRCm39)	splice site	probably null
R4242:Tle3	UTSW	9	61,314,705 (GRCm39)	missense	probably benign
R4587:Tle3	UTSW	9	61,281,295 (GRCm39)	missense	probably damaging	0.99
R4811:Tle3	UTSW	9	61,281,279 (GRCm39)	unclassified	probably benign
R4877:Tle3	UTSW	9	61,280,781 (GRCm39)	intron	probably benign
R4913:Tle3	UTSW	9	61,281,275 (GRCm39)	missense	probably damaging	1.00
R5387:Tle3	UTSW	9	61,314,771 (GRCm39)	splice site	probably null
R5745:Tle3	UTSW	9	61,322,133 (GRCm39)	missense	probably damaging	1.00
R5752:Tle3	UTSW	9	61,314,753 (GRCm39)	missense	probably damaging	1.00
R5917:Tle3	UTSW	9	61,316,190 (GRCm39)	missense	probably benign	0.19
R6000:Tle3	UTSW	9	61,281,296 (GRCm39)	missense	probably damaging	1.00
R6339:Tle3	UTSW	9	61,309,206 (GRCm39)	splice site	probably null
R7210:Tle3	UTSW	9	61,319,587 (GRCm39)	missense	probably damaging	1.00
R7460:Tle3	UTSW	9	61,320,366 (GRCm39)	missense	probably damaging	0.99
R7545:Tle3	UTSW	9	61,301,984 (GRCm39)	missense	possibly damaging	0.62
R7698:Tle3	UTSW	9	61,320,138 (GRCm39)	missense	probably damaging	1.00
R7916:Tle3	UTSW	9	61,314,410 (GRCm39)	missense	probably benign
R8075:Tle3	UTSW	9	61,281,841 (GRCm39)	missense	probably benign	0.05
R8941:Tle3	UTSW	9	61,320,195 (GRCm39)	missense	probably damaging	1.00
R9018:Tle3	UTSW	9	61,319,750 (GRCm39)	missense	probably damaging	1.00
R9060:Tle3	UTSW	9	61,282,821 (GRCm39)	missense	probably damaging	1.00
R9109:Tle3	UTSW	9	61,319,562 (GRCm39)	missense	possibly damaging	0.55
R9122:Tle3	UTSW	9	61,314,755 (GRCm39)	unclassified	probably benign
R9298:Tle3	UTSW	9	61,319,562 (GRCm39)	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- GCTGCCCTTGGAAAAGACATGACTC -3'
(R):5'- CGGGGATATGTACTATACTAACCCTGCC -3'

Sequencing Primer
(F):5'- CCTCAGCTTTGTCTATCAGAAGGG -3'
(R):5'- GTACTATACTAACCCTGCCAGCTC -3'

Posted On

2013-04-24