Mutagenetix > Incidental Mutation

Incidental Mutation 'R4190:Vmn2r84'

319927

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r84

Ensembl Gene

ENSMUSG00000070601

Gene Name

vomeronasal 2, receptor 84

Synonyms

EG625068

MMRRC Submission

041021-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R4190 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

130221669-130230110 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 130227163 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 225 (L225*)

Ref Sequence

ENSEMBL: ENSMUSP00000092079 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094502]

AlphaFold

D3YWE3

Predicted Effect

probably null
Transcript: ENSMUST00000094502
AA Change: L225*

SMART Domains

Protein: ENSMUSP00000092079
Gene: ENSMUSG00000070601
AA Change: L225*

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	77	448	1.3e-27	PFAM
Pfam:NCD3G	508	561	6.9e-21	PFAM
Pfam:7tm_3	594	830	4.6e-55	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

96% (50/52)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acot4	T	C	12: 84,089,948 (GRCm39)		probably benign	Het
Adgre1	A	G	17: 57,709,811 (GRCm39)	Y156C	unknown	Het
Ankrd13b	G	A	11: 77,367,201 (GRCm39)	A21V	probably damaging	Het
Atf7ip	T	C	6: 136,564,499 (GRCm39)	V914A	probably damaging	Het
C230029F24Rik	AGAAAG	A	1: 49,350,088 (GRCm39)		noncoding transcript	Het
Calcr	T	A	6: 3,717,106 (GRCm39)	D118V	possibly damaging	Het
Cdc42bpg	T	C	19: 6,371,711 (GRCm39)	V1330A	probably damaging	Het
Cfap54	T	C	10: 92,720,885 (GRCm39)	E2567G	unknown	Het
Ctse	T	A	1: 131,590,479 (GRCm39)	V67D	probably benign	Het
Ddx19b	A	T	8: 111,737,980 (GRCm39)	L256Q	probably damaging	Het
Dipk1b	T	C	2: 26,526,193 (GRCm39)	Y376H	probably damaging	Het
Fahd2a	C	T	2: 127,280,486 (GRCm39)	V156I	probably benign	Het
Fam185a	T	A	5: 21,630,122 (GRCm39)		probably benign	Het
Fam234a	A	T	17: 26,432,834 (GRCm39)	L467Q	probably damaging	Het
Gabra2	G	A	5: 71,165,341 (GRCm39)	P210S	probably benign	Het
Heatr3	T	C	8: 88,864,888 (GRCm39)		probably benign	Het
Herc2	T	C	7: 55,772,196 (GRCm39)	V1125A	probably benign	Het
Itga11	T	A	9: 62,639,391 (GRCm39)	C129S	probably damaging	Het
Lama2	A	G	10: 27,142,660 (GRCm39)	L606P	probably damaging	Het
Macf1	A	G	4: 123,366,835 (GRCm39)	F1077S	possibly damaging	Het
Mtmr10	G	T	7: 63,963,934 (GRCm39)	W223L	probably benign	Het
Nlgn1	T	A	3: 25,488,062 (GRCm39)	T758S	probably benign	Het
Nr4a1	T	C	15: 101,171,993 (GRCm39)	S556P	probably damaging	Het
Or4c126	C	A	2: 89,823,918 (GRCm39)	Y60*	probably null	Het
Or5p64	T	C	7: 107,855,330 (GRCm39)	D5G	probably benign	Het
Or6c201	T	C	10: 128,968,836 (GRCm39)	N267S	possibly damaging	Het
Or6c33	A	T	10: 129,853,847 (GRCm39)	I206F	probably damaging	Het
Pcdhgb8	G	C	18: 37,896,594 (GRCm39)	D555H	probably damaging	Het
Plscr5	T	C	9: 92,080,651 (GRCm39)	S46P	probably benign	Het
Ppp2r3d	T	C	9: 124,424,123 (GRCm38)		probably benign	Het
Prodh	C	T	16: 17,891,504 (GRCm39)	V480I	probably benign	Het
Senp2	G	T	16: 21,865,417 (GRCm39)	W580L	probably damaging	Het
Sorcs3	T	C	19: 48,737,812 (GRCm39)	V743A	possibly damaging	Het
Sp110	C	G	1: 85,516,839 (GRCm39)	E219D	probably damaging	Het
Stab2	G	T	10: 86,714,808 (GRCm39)	N119K	probably damaging	Het
Telo2	T	C	17: 25,320,987 (GRCm39)	T743A	probably benign	Het
Trav6-1	A	G	14: 52,875,976 (GRCm39)	N2S	probably benign	Het
Trmt44	C	A	5: 35,732,314 (GRCm39)	V26L	possibly damaging	Het
Ttbk1	A	T	17: 46,790,173 (GRCm39)	C91S	probably damaging	Het
Vit	A	G	17: 78,894,255 (GRCm39)	H219R	probably benign	Het
Vmn2r87	A	G	10: 130,308,556 (GRCm39)	Y561H	probably damaging	Het
Yars1	T	A	4: 129,093,820 (GRCm39)	C60*	probably null	Het
Zfp202	T	C	9: 40,122,633 (GRCm39)	V465A	probably benign	Het

Other mutations in Vmn2r84

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01461:Vmn2r84	APN	10	130,227,094 (GRCm39)	missense	possibly damaging	0.65
IGL01590:Vmn2r84	APN	10	130,221,964 (GRCm39)	missense	probably damaging	1.00
IGL01639:Vmn2r84	APN	10	130,225,141 (GRCm39)	nonsense	probably null
IGL01843:Vmn2r84	APN	10	130,222,148 (GRCm39)	missense	probably benign
IGL01911:Vmn2r84	APN	10	130,222,277 (GRCm39)	missense	probably damaging	0.99
IGL01937:Vmn2r84	APN	10	130,221,755 (GRCm39)	missense	probably damaging	1.00
IGL01977:Vmn2r84	APN	10	130,229,935 (GRCm39)	missense	probably benign	0.11
IGL02177:Vmn2r84	APN	10	130,227,881 (GRCm39)	missense	probably benign	0.00
IGL02291:Vmn2r84	APN	10	130,226,617 (GRCm39)	missense	probably damaging	1.00
IGL02590:Vmn2r84	APN	10	130,227,356 (GRCm39)	splice site	probably benign
IGL02727:Vmn2r84	APN	10	130,229,995 (GRCm39)	missense	possibly damaging	0.95
IGL02900:Vmn2r84	APN	10	130,223,861 (GRCm39)	splice site	probably benign
IGL03383:Vmn2r84	APN	10	130,222,556 (GRCm39)	missense	probably damaging	1.00
PIT4378001:Vmn2r84	UTSW	10	130,221,784 (GRCm39)	missense	probably damaging	1.00
R0076:Vmn2r84	UTSW	10	130,230,062 (GRCm39)	missense	probably damaging	1.00
R0089:Vmn2r84	UTSW	10	130,222,588 (GRCm39)	splice site	probably benign
R0153:Vmn2r84	UTSW	10	130,227,877 (GRCm39)	missense	probably benign	0.06
R0611:Vmn2r84	UTSW	10	130,221,991 (GRCm39)	missense	probably damaging	1.00
R0883:Vmn2r84	UTSW	10	130,226,984 (GRCm39)	missense	probably damaging	0.99
R1237:Vmn2r84	UTSW	10	130,223,725 (GRCm39)	splice site	probably null
R1295:Vmn2r84	UTSW	10	130,225,008 (GRCm39)	missense	probably benign	0.12
R1401:Vmn2r84	UTSW	10	130,227,859 (GRCm39)	missense	possibly damaging	0.89
R1521:Vmn2r84	UTSW	10	130,225,137 (GRCm39)	missense	probably benign	0.10
R1590:Vmn2r84	UTSW	10	130,227,349 (GRCm39)	critical splice acceptor site	probably null
R1710:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R1891:Vmn2r84	UTSW	10	130,221,938 (GRCm39)	missense	possibly damaging	0.78
R1956:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1957:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1962:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
R1994:Vmn2r84	UTSW	10	130,221,878 (GRCm39)	missense	probably damaging	1.00
R2124:Vmn2r84	UTSW	10	130,227,100 (GRCm39)	missense	probably damaging	0.99
R2409:Vmn2r84	UTSW	10	130,227,940 (GRCm39)	missense	probably damaging	0.99
R2474:Vmn2r84	UTSW	10	130,222,392 (GRCm39)	missense	possibly damaging	0.50
R2851:Vmn2r84	UTSW	10	130,230,036 (GRCm39)	missense	probably benign	0.05
R3508:Vmn2r84	UTSW	10	130,226,777 (GRCm39)	missense	probably damaging	1.00
R3792:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R4051:Vmn2r84	UTSW	10	130,226,767 (GRCm39)	missense	probably damaging	1.00
R4061:Vmn2r84	UTSW	10	130,221,898 (GRCm39)	missense	probably damaging	1.00
R4091:Vmn2r84	UTSW	10	130,227,238 (GRCm39)	missense	probably damaging	1.00
R4520:Vmn2r84	UTSW	10	130,222,391 (GRCm39)	missense	probably damaging	1.00
R4584:Vmn2r84	UTSW	10	130,226,582 (GRCm39)	missense	probably benign	0.00
R4588:Vmn2r84	UTSW	10	130,221,809 (GRCm39)	missense	probably damaging	0.98
R4655:Vmn2r84	UTSW	10	130,229,973 (GRCm39)	nonsense	probably null
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R5022:Vmn2r84	UTSW	10	130,222,417 (GRCm39)	missense	probably damaging	1.00
R5146:Vmn2r84	UTSW	10	130,221,971 (GRCm39)	missense	probably damaging	1.00
R5237:Vmn2r84	UTSW	10	130,221,863 (GRCm39)	missense	probably damaging	0.99
R5695:Vmn2r84	UTSW	10	130,225,064 (GRCm39)	missense	probably benign	0.12
R5793:Vmn2r84	UTSW	10	130,221,754 (GRCm39)	missense	probably damaging	0.99
R6210:Vmn2r84	UTSW	10	130,222,114 (GRCm39)	missense	probably damaging	1.00
R6286:Vmn2r84	UTSW	10	130,226,737 (GRCm39)	missense	possibly damaging	0.65
R6580:Vmn2r84	UTSW	10	130,225,110 (GRCm39)	missense	possibly damaging	0.93
R6607:Vmn2r84	UTSW	10	130,226,731 (GRCm39)	missense	possibly damaging	0.87
R6818:Vmn2r84	UTSW	10	130,222,147 (GRCm39)	missense	probably benign	0.09
R6956:Vmn2r84	UTSW	10	130,225,136 (GRCm39)	missense	probably damaging	0.98
R6994:Vmn2r84	UTSW	10	130,226,876 (GRCm39)	missense	possibly damaging	0.90
R7075:Vmn2r84	UTSW	10	130,226,941 (GRCm39)	missense	probably damaging	0.99
R7225:Vmn2r84	UTSW	10	130,222,552 (GRCm39)	missense	probably damaging	0.99
R7252:Vmn2r84	UTSW	10	130,222,279 (GRCm39)	missense	probably damaging	1.00
R7263:Vmn2r84	UTSW	10	130,225,077 (GRCm39)	missense	probably damaging	1.00
R7297:Vmn2r84	UTSW	10	130,227,119 (GRCm39)	missense	probably benign	0.19
R7439:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7441:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7857:Vmn2r84	UTSW	10	130,226,738 (GRCm39)	missense	probably benign	0.00
R8263:Vmn2r84	UTSW	10	130,227,037 (GRCm39)	missense	probably damaging	1.00
R8555:Vmn2r84	UTSW	10	130,230,100 (GRCm39)	missense	probably benign	0.28
R8766:Vmn2r84	UTSW	10	130,222,241 (GRCm39)	missense	probably damaging	0.98
R8821:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8831:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8970:Vmn2r84	UTSW	10	130,222,244 (GRCm39)	missense	probably damaging	0.98
R9164:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R9190:Vmn2r84	UTSW	10	130,226,843 (GRCm39)	missense	probably benign	0.03
R9261:Vmn2r84	UTSW	10	130,229,976 (GRCm39)	missense	probably benign	0.00
R9310:Vmn2r84	UTSW	10	130,227,993 (GRCm39)	missense	possibly damaging	0.81
R9434:Vmn2r84	UTSW	10	130,221,745 (GRCm39)	missense	possibly damaging	0.65
R9613:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r84	UTSW	10	130,227,771 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCATTGTGAGGTTGTGATCCAG -3'
(R):5'- GACCATTTAATCCTAAACTGAGTGACC -3'

Sequencing Primer
(F):5'- GAGGTTGTGATCCAGATCCTCC -3'
(R):5'- AACTGAGTGACCATGACCATTTTCC -3'

Posted On

2015-06-10