Mutagenetix > Incidental Mutation

Incidental Mutation 'R4166:Gm5174'

320592

Institutional Source

Beutler Lab

Gene Symbol

Gm5174

Ensembl Gene

ENSMUSG00000090308

Gene Name

predicted gene 5174

Synonyms

MMRRC Submission

041639-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.102) question?

Stock #

R4166 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

86491822-86493244 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

A to G at 86492797 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000171131

SMART Domains

Protein: ENSMUSP00000129952
Gene: ENSMUSG00000090308

Domain	Start	End	E-Value	Type
S_TKc	14	262	5.59e-86	SMART
UBA	276	313	1.28e-3	SMART
low complexity region	447	462	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219608

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.8%

Validation Efficiency

100% (56/56)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	T	C	14: 32,382,912 (GRCm39)	T1018A	possibly damaging	Het
Aak1	T	A	6: 86,827,044 (GRCm39)	F4I	probably damaging	Het
Adamts8	G	T	9: 30,862,684 (GRCm39)	E296D	probably benign	Het
Ankrd35	T	A	3: 96,586,471 (GRCm39)		probably null	Het
Arhgap20	G	A	9: 51,738,135 (GRCm39)		probably null	Het
Bhmt	T	A	13: 93,762,007 (GRCm39)		probably benign	Het
Cacna1b	C	A	2: 24,567,923 (GRCm39)	R1055L	probably benign	Het
Cdk5r1	A	G	11: 80,369,035 (GRCm39)	Y234C	probably damaging	Het
Cdx2	G	T	5: 147,243,539 (GRCm39)	A85D	possibly damaging	Het
Cenpe	G	A	3: 134,949,479 (GRCm39)	G88D	probably damaging	Het
Cfap300	A	T	9: 8,026,071 (GRCm39)	L167Q	probably damaging	Het
Dennd5a	A	C	7: 109,526,032 (GRCm39)		probably null	Het
Erich6	G	A	3: 58,526,229 (GRCm39)	A591V	probably damaging	Het
Espl1	A	G	15: 102,221,424 (GRCm39)	I944V	probably damaging	Het
Fbln5	A	G	12: 101,723,618 (GRCm39)	V374A	probably damaging	Het
Fmn1	G	A	2: 113,467,080 (GRCm39)	S1327N	probably benign	Het
Fnip2	A	T	3: 79,369,442 (GRCm39)	V1081E	probably damaging	Het
Gpaa1	A	C	15: 76,216,667 (GRCm39)		probably benign	Het
Grina	T	A	15: 76,133,529 (GRCm39)	L334Q	probably damaging	Het
Gulp1	T	A	1: 44,747,829 (GRCm39)	Y27*	probably null	Het
Kdm3b	A	T	18: 34,928,797 (GRCm39)	I183F	probably benign	Het
Loxhd1	A	T	18: 77,460,025 (GRCm39)	I758F	probably damaging	Het
Msi2	T	C	11: 88,237,914 (GRCm39)	H346R	probably benign	Het
Naip6	T	A	13: 100,452,657 (GRCm39)	I135F	probably benign	Het
Npr3	T	G	15: 11,848,599 (GRCm39)	E202A	probably benign	Het
Nsun4	A	T	4: 115,891,248 (GRCm39)	L377Q	probably damaging	Het
Oog2	A	C	4: 143,921,411 (GRCm39)	H107P	probably damaging	Het
Or14c40	C	A	7: 86,313,602 (GRCm39)	T244N	probably damaging	Het
Otof	A	G	5: 30,539,762 (GRCm39)	L1032P	probably damaging	Het
Pcdh9	T	A	14: 94,124,956 (GRCm39)	R405*	probably null	Het
Pcdhb19	T	A	18: 37,632,243 (GRCm39)	N679K	probably benign	Het
Pigr	G	A	1: 130,769,554 (GRCm39)	D122N	probably benign	Het
Prol1	A	T	5: 88,476,530 (GRCm39)	I307F	unknown	Het
Pycr1	T	C	11: 120,532,949 (GRCm39)	I104V	probably benign	Het
Ralgapa1	C	T	12: 55,687,429 (GRCm39)	R2019Q	probably damaging	Het
Rb1cc1	T	C	1: 6,335,887 (GRCm39)		probably benign	Het
Skint11	C	A	4: 114,101,856 (GRCm39)	Q99K	probably benign	Het
Skint3	A	G	4: 112,112,832 (GRCm39)	I147M	possibly damaging	Het
Slc22a14	T	G	9: 119,007,498 (GRCm39)	M304L	probably benign	Het
Slc22a14	T	C	9: 119,008,934 (GRCm39)	N178S	possibly damaging	Het
Slc2a1	T	G	4: 118,990,313 (GRCm39)	I179S	probably damaging	Het
Slco2b1	G	T	7: 99,309,333 (GRCm39)	T666N	probably benign	Het
Smap1	T	C	1: 23,887,506 (GRCm39)	M258V	probably benign	Het
Smr3a	A	T	5: 88,156,013 (GRCm39)		probably benign	Het
Stt3b	T	A	9: 115,083,969 (GRCm39)	E402D	probably damaging	Het
Sync	T	C	4: 129,200,535 (GRCm39)		probably benign	Het
Tbx20	T	A	9: 24,681,040 (GRCm39)	I151F	probably damaging	Het
Tomm22	C	A	15: 79,555,206 (GRCm39)		probably benign	Het
Trank1	C	A	9: 111,202,592 (GRCm39)	Y1652*	probably null	Het
Trim34a	T	A	7: 103,910,223 (GRCm39)	C342S	probably benign	Het
Txnl4a	T	A	18: 80,265,471 (GRCm39)	M112K	probably benign	Het
Zfp146	A	G	7: 29,861,865 (GRCm39)	V59A	possibly damaging	Het

Other mutations in Gm5174

Allele	Source	Chr	Coord	Type	Predicted Effect
Laco	UTSW	10	86,491,972 (GRCm39)	unclassified	noncoding transcript
R1083:Gm5174	UTSW	10	86,491,972 (GRCm39)	unclassified	noncoding transcript
R1199:Gm5174	UTSW	10	86,493,189 (GRCm39)	unclassified	noncoding transcript
R1296:Gm5174	UTSW	10	86,492,866 (GRCm39)	unclassified	noncoding transcript
R1715:Gm5174	UTSW	10	86,492,776 (GRCm39)	unclassified	noncoding transcript
R1957:Gm5174	UTSW	10	86,492,617 (GRCm39)	unclassified	noncoding transcript
R2221:Gm5174	UTSW	10	86,492,372 (GRCm39)	unclassified	noncoding transcript
R2223:Gm5174	UTSW	10	86,492,372 (GRCm39)	unclassified	noncoding transcript
R3104:Gm5174	UTSW	10	86,492,519 (GRCm39)	unclassified	noncoding transcript
R4165:Gm5174	UTSW	10	86,492,797 (GRCm39)	unclassified	noncoding transcript
R4243:Gm5174	UTSW	10	86,492,144 (GRCm39)	unclassified	noncoding transcript
R4244:Gm5174	UTSW	10	86,492,144 (GRCm39)	unclassified	noncoding transcript
R5024:Gm5174	UTSW	10	86,492,451 (GRCm39)	unclassified	noncoding transcript
R5292:Gm5174	UTSW	10	86,492,562 (GRCm39)	unclassified	noncoding transcript
R5586:Gm5174	UTSW	10	86,492,409 (GRCm39)	unclassified	noncoding transcript
R5864:Gm5174	UTSW	10	86,493,045 (GRCm39)	unclassified	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- CATTGAGAAACATCCTTGGGTC -3'
(R):5'- AGGCATGGAGTCACTTCTGG -3'

Sequencing Primer
(F):5'- ACATCCTTGGGTCACGAAATG -3'
(R):5'- GCATGGAGTCACTTCTGGTCAAC -3'

Posted On

2015-06-12