Mutagenetix > Incidental Mutation

Incidental Mutation 'R4170:Lysmd3'

320746

Institutional Source

Beutler Lab

Gene Symbol

Lysmd3

Ensembl Gene

ENSMUSG00000035840

Gene Name

LysM, putative peptidoglycan-binding, domain containing 3

Synonyms

1110030H10Rik

MMRRC Submission

040863-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R4170 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

81805782-81820990 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 81817529 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 169 (Q169*)

Ref Sequence

ENSEMBL: ENSMUSP00000152940 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048993] [ENSMUST00000049055] [ENSMUST00000224300] [ENSMUST00000224592]

AlphaFold

Q99LE3

Predicted Effect

probably benign
Transcript: ENSMUST00000048993

SMART Domains

Protein: ENSMUSP00000035289
Gene: ENSMUSG00000035834

Domain	Start	End	E-Value	Type
Pfam:RNA_pol_3_Rpc31	1	223	7e-41	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000049055

SMART Domains

Protein: ENSMUSP00000038275
Gene: ENSMUSG00000035840

Domain	Start	End	E-Value	Type
LysM	66	110	4.6e-4	SMART
low complexity region	149	158	N/A	INTRINSIC
transmembrane domain	214	236	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162535

Predicted Effect

probably null
Transcript: ENSMUST00000224300
AA Change: Q169*

Predicted Effect

probably benign
Transcript: ENSMUST00000224420

Predicted Effect

probably benign
Transcript: ENSMUST00000224592

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.5%
20x: 96.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700047A11Rik	T	A	8: 26,585,815 (GRCm39)	T63S	probably benign	Het
Acap3	T	A	4: 155,984,458 (GRCm39)	S189T	possibly damaging	Het
Acsbg2	G	A	17: 57,160,846 (GRCm39)	T266I	probably benign	Het
Cacna1s	A	G	1: 136,035,933 (GRCm39)	D1144G	probably damaging	Het
Diaph3	T	C	14: 87,223,143 (GRCm39)	D365G	probably damaging	Het
Dock7	T	A	4: 98,854,638 (GRCm39)	D1542V	probably damaging	Het
Efr3a	T	A	15: 65,717,831 (GRCm39)	V337D	probably damaging	Het
Elk3	A	G	10: 93,101,197 (GRCm39)		probably null	Het
Ercc6	T	C	14: 32,288,754 (GRCm39)	L867P	probably damaging	Het
Gdf6	A	G	4: 9,859,650 (GRCm39)	D244G	probably benign	Het
Get1	G	A	16: 95,954,176 (GRCm39)	A92T	probably benign	Het
Gzmn	T	C	14: 56,404,261 (GRCm39)	D192G	possibly damaging	Het
Krt81	T	A	15: 101,359,193 (GRCm39)	M242L	probably benign	Het
Man2c1	A	G	9: 57,045,310 (GRCm39)	D473G	probably benign	Het
Mmp23	C	T	4: 155,735,767 (GRCm39)	R268Q	probably damaging	Het
Or1j17	C	A	2: 36,578,734 (GRCm39)	T240N	probably damaging	Het
Or5p58	T	A	7: 107,694,280 (GRCm39)	I166L	probably benign	Het
Prmt3	G	T	7: 49,476,524 (GRCm39)	A378S	probably benign	Het
Ptf1a	C	T	2: 19,451,819 (GRCm39)	L273F	possibly damaging	Het
Ptpru	A	T	4: 131,503,659 (GRCm39)	Y1124N	probably damaging	Het
Rtkn	T	G	6: 83,119,376 (GRCm39)	M1R	probably null	Het
Rttn	T	A	18: 88,993,847 (GRCm39)	F175I	probably damaging	Het
Slc5a7	T	C	17: 54,583,886 (GRCm39)	D468G	probably benign	Het
Slc9a9	A	G	9: 95,110,952 (GRCm39)	Y590C	probably damaging	Het
Smarcd1	T	C	15: 99,605,812 (GRCm39)	L320P	probably damaging	Het
Sox2	A	T	3: 34,704,703 (GRCm39)	M47L	probably damaging	Het
Sptan1	T	C	2: 29,920,037 (GRCm39)	M2258T	possibly damaging	Het
Tnrc6b	T	A	15: 80,800,988 (GRCm39)	D1431E	probably benign	Het
Trav8n-2	A	T	14: 53,583,875 (GRCm39)	T111S	possibly damaging	Het
Tut4	A	G	4: 108,405,256 (GRCm39)	Y1293C	probably damaging	Het
Zbtb46	T	C	2: 181,066,148 (GRCm39)	M1V	probably null	Het
Zfp7	T	C	15: 76,775,818 (GRCm39)	V620A	probably benign	Het
Zfp804a	T	A	2: 82,083,832 (GRCm39)	F95I	probably damaging	Het

Other mutations in Lysmd3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00326:Lysmd3	APN	13	81,813,363 (GRCm39)	missense	probably damaging	1.00
IGL00327:Lysmd3	APN	13	81,813,197 (GRCm39)	missense	probably benign	0.00
IGL00813:Lysmd3	APN	13	81,813,361 (GRCm39)	missense	probably damaging	1.00
R1509:Lysmd3	UTSW	13	81,817,390 (GRCm39)	missense	probably benign	0.11
R2158:Lysmd3	UTSW	13	81,817,737 (GRCm39)	missense	probably damaging	1.00
R4720:Lysmd3	UTSW	13	81,817,584 (GRCm39)	missense	possibly damaging	0.81
R5120:Lysmd3	UTSW	13	81,817,311 (GRCm39)	missense	probably damaging	1.00
R5728:Lysmd3	UTSW	13	81,817,380 (GRCm39)	missense	probably damaging	1.00
R5979:Lysmd3	UTSW	13	81,813,393 (GRCm39)	splice site	probably null
R5990:Lysmd3	UTSW	13	81,817,707 (GRCm39)	missense	probably damaging	1.00
R8223:Lysmd3	UTSW	13	81,817,386 (GRCm39)	missense
R9013:Lysmd3	UTSW	13	81,817,580 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCTCTTAGGTCTATCAAAATTCCAG -3'
(R):5'- AGAACCCACTGTTGAATGGTG -3'

Sequencing Primer
(F):5'- CCAGTTAAAAGGTTTAGTTCTTTGAC -3'
(R):5'- CCCACTGTTGAATGGTGACTAAC -3'

Posted On

2015-06-12