Mutagenetix > Incidental Mutations

Incidental Mutation 'R4230:Gm2035'

320802

Institutional Source

Beutler Lab

Gene Symbol

Gm2035

Ensembl Gene

ENSMUSG00000096619

Gene Name

predicted pseudogene 2035

Synonyms

MMRRC Submission

041049-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.222) question?

Stock #

R4230 (G1)

Quality Score

102

Status

Not validated

Chromosome

Chromosomal Location

87919423-87919857 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 87919797 bp

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 21 (S21T)

Ref Sequence

ENSEMBL: ENSMUSP00000137146 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000180053]

Predicted Effect

unknown
Transcript: ENSMUST00000180053
AA Change: S21T

SMART Domains

Protein: ENSMUSP00000137146
Gene: ENSMUSG00000096619
AA Change: S21T

Domain	Start	End	E-Value	Type
low complexity region	3	25	N/A	INTRINSIC
eIF1a	28	110	4.13e-45	SMART
low complexity region	125	144	N/A	INTRINSIC

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	C	T	6: 128,580,386	A115T	probably benign	Het
Alg11	T	A	8: 22,065,518	I223N	probably damaging	Het
Ankrd26	G	C	6: 118,559,388		probably null	Het
Apbb1	T	C	7: 105,567,684	D264G	probably damaging	Het
Arhgap32	T	C	9: 32,257,474	Y918H	probably benign	Het
Astn2	T	G	4: 65,911,682	T584P	probably damaging	Het
Atxn7	G	A	14: 14,100,381	S689N	probably benign	Het
Cacna1h	T	C	17: 25,387,863	N873S	probably damaging	Het
Cadps	T	A	14: 12,488,987	I857F	probably damaging	Het
Ccdc189	T	A	7: 127,586,830	M102L	probably benign	Het
Cog6	T	C	3: 52,992,808	T511A	probably benign	Het
Dppa3	A	T	6: 122,629,332	N118I	probably damaging	Het
Dst	A	G	1: 34,195,828	N3663D	probably benign	Het
Eme1	G	A	11: 94,647,992	T354M	possibly damaging	Het
Gm10735	T	C	13: 113,041,209		probably benign	Het
Ifi44l	A	T	3: 151,762,877	C5*	probably null	Het
Itln1	G	A	1: 171,534,807	T2I	probably benign	Het
Jam2	T	A	16: 84,821,292	M309K	possibly damaging	Het
Kcnk5	C	A	14: 20,144,784	C162F	probably damaging	Het
Metrn	A	G	17: 25,796,941		probably benign	Het
Muc5b	T	A	7: 141,863,522	Y3402N	probably benign	Het
Ncoa7	A	T	10: 30,698,257		probably null	Het
Nxpe4	A	G	9: 48,392,822	R70G	possibly damaging	Het
Olfr1281	T	G	2: 111,329,130	L237R	probably damaging	Het
Olfr134	A	C	17: 38,175,881	I266L	possibly damaging	Het
Olfr373	T	C	8: 72,100,344	Y195H	probably damaging	Het
Olfr670	A	T	7: 104,960,594	I46N	probably benign	Het
Onecut3	T	C	10: 80,513,959	I429T	probably damaging	Het
Pds5a	A	G	5: 65,629,986	S858P	possibly damaging	Het
Phf11c	A	T	14: 59,393,067	V63E	probably benign	Het
Rasa3	A	G	8: 13,570,264	F802L	possibly damaging	Het
Sec24b	G	T	3: 130,040,719	Q141K	probably benign	Het
Sec61a1	A	T	6: 88,515,431		probably null	Het
Slc45a3	T	A	1: 131,981,661	L532Q	probably damaging	Het
Slc46a2	T	C	4: 59,914,048	I292V	probably benign	Het
Smg1	C	T	7: 118,148,733		probably null	Het
Spag6	T	A	2: 18,715,638		probably null	Het
Spi1	T	C	2: 91,115,335	F254L	probably damaging	Het
Sspo	T	C	6: 48,490,934	S4272P	probably benign	Het
Tgm5	T	C	2: 121,070,735	T292A	probably damaging	Het
Tmem229a	T	A	6: 24,954,832	I308F	probably damaging	Het
Ttn	T	C	2: 76,724,120	I30784V	possibly damaging	Het
Urod	T	A	4: 116,992,683	T173S	probably benign	Het
Vmn1r223	A	T	13: 23,249,415	M60L	probably benign	Het
Vmn2r2	T	A	3: 64,134,491	I268L	probably benign	Het
Vwa8	C	T	14: 79,082,852	T1101M	probably benign	Het
Zc3h12c	T	A	9: 52,144,428		probably null	Het

Other mutations in Gm2035

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02314:Gm2035	APN	12	87919607	missense	probably benign	0.00
IGL02347:Gm2035	APN	12	87919589	missense	probably damaging	1.00
IGL02639:Gm2035	APN	12	87919499	missense	probably benign	0.04
R5969:Gm2035	UTSW	12	87919478	missense	unknown
R6159:Gm2035	UTSW	12	87919751	missense	probably damaging	1.00
R6359:Gm2035	UTSW	12	87919505	missense	probably benign	0.09
R7181:Gm2035	UTSW	12	87919722	missense	possibly damaging	0.49
R7183:Gm2035	UTSW	12	87919722	missense	possibly damaging	0.49
R7184:Gm2035	UTSW	12	87919722	missense	possibly damaging	0.49
R7653:Gm2035	UTSW	12	87919478	missense	unknown
R8046:Gm2035	UTSW	12	87919565	missense	probably benign	0.02
R8353:Gm2035	UTSW	12	87919553	missense	probably benign
R8453:Gm2035	UTSW	12	87919553	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- AGGCCTTCAGACTTCTTGC -3'
(R):5'- GATACTTCCTGGCACCCATC -3'

Sequencing Primer
(F):5'- ACATCAGCTTTGTTATCTTGATAGTC -3'
(R):5'- CCTAAAACTGTTGGAACTGAGTCAC -3'

Posted On

2015-06-12