Mutagenetix > Incidental Mutation

Incidental Mutation 'R4232:Olfr1535'

320923

Institutional Source

Beutler Lab

Gene Symbol

Olfr1535

Ensembl Gene

ENSMUSG00000054890

Gene Name

olfactory receptor 1535

Synonyms

MOR256-36, Olfr1365, MOR256-63, GA_x6K02T2QHY8-11688984-11689964, MOR256-36

MMRRC Submission

041051-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R4232 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

21553894-21559893 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 21555461 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 187 (D187G)

Ref Sequence

ENSEMBL: ENSMUSP00000149589 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068163] [ENSMUST00000217519]

AlphaFold

Q7TQU0

Predicted Effect

probably damaging
Transcript: ENSMUST00000068163
AA Change: D187G

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000064142
Gene: ENSMUSG00000054890
AA Change: D187G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	320	5.4e-46	PFAM
Pfam:7tm_1	41	302	1.3e-19	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000217519
AA Change: D187G

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.9%

Validation Efficiency

92% (58/63)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930567H17Rik	C	T	X: 70,394,529 (GRCm38)	A53T	probably benign	Het
Ajuba	T	C	14: 54,569,526 (GRCm38)	R490G	probably damaging	Het
Akap6	T	A	12: 53,139,671 (GRCm38)	N1289K	probably damaging	Het
Arhgap21	A	G	2: 20,887,137 (GRCm38)	V161A	probably damaging	Het
Atg14	T	C	14: 47,551,345 (GRCm38)	K184E	probably benign	Het
Aven	A	G	2: 112,627,768 (GRCm38)	D167G	probably damaging	Het
Celsr2	A	G	3: 108,413,772 (GRCm38)	F575L	probably benign	Het
Cflar	A	T	1: 58,740,993 (GRCm38)	Q249L	possibly damaging	Het
Col7a1	G	A	9: 108,972,813 (GRCm38)		probably null	Het
Dmxl2	T	C	9: 54,419,909 (GRCm38)	D944G	possibly damaging	Het
Dnah1	T	C	14: 31,304,916 (GRCm38)	N717S	probably benign	Het
Dnaja3	T	A	16: 4,699,871 (GRCm38)	N322K	possibly damaging	Het
Dnajb3	A	G	1: 88,205,252 (GRCm38)	S143P	possibly damaging	Het
Dtx3	A	G	10: 127,193,189 (GRCm38)	I60T	possibly damaging	Het
Dvl3	A	G	16: 20,524,233 (GRCm38)		probably benign	Het
Fam71b	C	A	11: 46,407,405 (GRCm38)	T512K	possibly damaging	Het
Fkbp7	A	T	2: 76,663,317 (GRCm38)	D177E	possibly damaging	Het
Galnt7	T	C	8: 57,652,966 (GRCm38)	I5V	probably benign	Het
Helz2	A	T	2: 181,229,902 (GRCm38)	L2639Q	probably damaging	Het
Hnrnpr	T	C	4: 136,339,189 (GRCm38)	M394T	probably benign	Het
Ip6k2	G	A	9: 108,805,648 (GRCm38)	R319Q	probably benign	Het
Kif14	T	A	1: 136,516,363 (GRCm38)	C1364*	probably null	Het
Macf1	T	C	4: 123,432,392 (GRCm38)	E5104G	probably damaging	Het
Mkl1	T	A	15: 81,023,595 (GRCm38)	K29M	probably damaging	Het
Mrps30	T	C	13: 118,386,840 (GRCm38)	D132G	probably damaging	Het
Mtbp	CATGA	CATGAATGA	15: 55,620,677 (GRCm38)		probably null	Het
Nfkb1	A	G	3: 135,603,770 (GRCm38)	V521A	probably damaging	Het
Olfr850	G	T	9: 19,477,726 (GRCm38)	L175M	probably damaging	Het
P2rx6	T	C	16: 17,570,767 (GRCm38)	L335P	probably damaging	Het
Pcdhga5	A	G	18: 37,695,948 (GRCm38)	D483G	possibly damaging	Het
Pgpep1l	T	C	7: 68,237,079 (GRCm38)	T161A	probably benign	Het
Prss40	A	G	1: 34,560,792 (GRCm38)	V38A	probably benign	Het
Ptk2	C	A	15: 73,309,849 (GRCm38)	R104L	possibly damaging	Het
Ralgapa1	C	T	12: 55,640,644 (GRCm38)	R2019Q	probably damaging	Het
Rin2	C	T	2: 145,860,446 (GRCm38)	T354I	probably benign	Het
Rtp4	A	G	16: 23,613,083 (GRCm38)	N122D	possibly damaging	Het
Samd8	A	G	14: 21,780,145 (GRCm38)	Y213C	probably benign	Het
Shcbp1	T	A	8: 4,736,372 (GRCm38)	T577S	probably benign	Het
Tiprl	A	G	1: 165,222,587 (GRCm38)	V153A	probably damaging	Het
Tmprss7	A	T	16: 45,656,573 (GRCm38)	D775E	probably damaging	Het
Tnr	T	C	1: 159,886,215 (GRCm38)	S738P	possibly damaging	Het
Tsc22d4	T	C	5: 137,751,370 (GRCm38)		probably null	Het
Ttc21a	A	T	9: 119,942,618 (GRCm38)	H161L	probably benign	Het
Ubqln3	T	A	7: 104,141,803 (GRCm38)	E360V	probably benign	Het
Ugt1a10	C	A	1: 88,056,210 (GRCm38)	D243E	probably benign	Het
Vmn2r19	G	A	6: 123,329,912 (GRCm38)	V460I	probably benign	Het
Vmn2r58	T	A	7: 41,837,587 (GRCm38)	Y628F	possibly damaging	Het
Wnk1	A	T	6: 119,949,261 (GRCm38)	S1588T	possibly damaging	Het
Wscd2	A	G	5: 113,560,984 (GRCm38)	D200G	probably benign	Het
Zfp831	G	C	2: 174,705,654 (GRCm38)	W1543C	possibly damaging	Het

Other mutations in Olfr1535

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01593:Olfr1535	APN	13	21,555,219 (GRCm38)	missense	probably damaging	0.98
IGL01866:Olfr1535	APN	13	21,555,173 (GRCm38)	missense	probably benign	0.07
IGL02289:Olfr1535	APN	13	21,555,588 (GRCm38)	missense	probably benign	0.02
IGL02350:Olfr1535	APN	13	21,555,602 (GRCm38)	missense	probably damaging	1.00
IGL02357:Olfr1535	APN	13	21,555,602 (GRCm38)	missense	probably damaging	1.00
IGL02793:Olfr1535	APN	13	21,556,002 (GRCm38)	missense	probably damaging	1.00
IGL02875:Olfr1535	APN	13	21,556,002 (GRCm38)	missense	probably damaging	1.00
R0785:Olfr1535	UTSW	13	21,555,788 (GRCm38)	missense	probably benign	0.02
R1773:Olfr1535	UTSW	13	21,555,812 (GRCm38)	missense	probably damaging	1.00
R3429:Olfr1535	UTSW	13	21,555,805 (GRCm38)	nonsense	probably null
R3430:Olfr1535	UTSW	13	21,555,805 (GRCm38)	nonsense	probably null
R4883:Olfr1535	UTSW	13	21,555,488 (GRCm38)	missense	probably benign	0.22
R5586:Olfr1535	UTSW	13	21,555,096 (GRCm38)	missense	probably damaging	1.00
R6032:Olfr1535	UTSW	13	21,555,907 (GRCm38)	missense	probably benign	0.01
R6032:Olfr1535	UTSW	13	21,555,907 (GRCm38)	missense	probably benign	0.01
R6542:Olfr1535	UTSW	13	21,555,507 (GRCm38)	missense	probably damaging	0.99
R7014:Olfr1535	UTSW	13	21,555,938 (GRCm38)	missense	probably benign	0.02
R7579:Olfr1535	UTSW	13	21,556,006 (GRCm38)	missense	probably benign	0.08
R7598:Olfr1535	UTSW	13	21,555,188 (GRCm38)	missense	probably damaging	1.00
R7982:Olfr1535	UTSW	13	21,555,966 (GRCm38)	missense	probably benign
R8239:Olfr1535	UTSW	13	21,555,618 (GRCm38)	missense	probably benign	0.01
R8339:Olfr1535	UTSW	13	21,555,826 (GRCm38)	missense	probably damaging	0.99
R8698:Olfr1535	UTSW	13	21,555,720 (GRCm38)	missense	probably damaging	1.00
R8956:Olfr1535	UTSW	13	21,555,999 (GRCm38)	missense	probably benign	0.00
R8977:Olfr1535	UTSW	13	21,555,846 (GRCm38)	missense	possibly damaging	0.57
RF051:Olfr1535	UTSW	13	21,555,523 (GRCm38)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- ACAGGTTCCAAAGGCCTTCC -3'
(R):5'- ATGATCGCTATGCTGCTGTCTG -3'

Sequencing Primer
(F):5'- CGCCTTCCCTGTGCTGAC -3'
(R):5'- ATGCTGCTGTCTGCCGAC -3'

Posted On

2015-06-12