Incidental Mutation 'R4233:Rufy4'
ID 320939
Institutional Source Beutler Lab
Gene Symbol Rufy4
Ensembl Gene ENSMUSG00000061815
Gene Name RUN and FYVE domain containing 4
Synonyms F930048N03Rik
MMRRC Submission 041642-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.202) question?
Stock # R4233 (G1)
Quality Score 225
Status Validated
Chromosome 1
Chromosomal Location 74164700-74187382 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 74186822 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Cysteine to Arginine at position 537 (C537R)
Ref Sequence ENSEMBL: ENSMUSP00000115873 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000080167] [ENSMUST00000127134]
AlphaFold Q3TYX8
Predicted Effect probably damaging
Transcript: ENSMUST00000080167
AA Change: C453R

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000079062
Gene: ENSMUSG00000061815
AA Change: C453R

DomainStartEndE-ValueType
Pfam:RUN 2 81 1.5e-8 PFAM
coiled coil region 331 404 N/A INTRINSIC
Blast:FYVE 415 472 2e-6 BLAST
SCOP:d1vfya_ 428 473 4e-7 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000127134
AA Change: C537R

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000115873
Gene: ENSMUSG00000061815
AA Change: C537R

DomainStartEndE-ValueType
Pfam:RUN 41 165 6.2e-10 PFAM
coiled coil region 415 488 N/A INTRINSIC
Blast:FYVE 499 556 2e-6 BLAST
SCOP:d1vfya_ 512 557 3e-7 SMART
Meta Mutation Damage Score 0.8605 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.8%
  • 10x: 97.6%
  • 20x: 96.2%
Validation Efficiency 100% (53/53)
Allele List at MGI
Other mutations in this stock
Total: 49 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700123K08Rik A T 5: 138,562,454 (GRCm39) Y96N probably damaging Het
Abca15 C T 7: 120,002,202 (GRCm39) Q1572* probably null Het
Ajuba T C 14: 54,806,983 (GRCm39) R490G probably damaging Het
Akap6 T A 12: 53,186,454 (GRCm39) N1289K probably damaging Het
Ankfy1 G A 11: 72,605,310 (GRCm39) probably null Het
Aph1c A G 9: 66,740,603 (GRCm39) F41S probably damaging Het
Arhgap21 A G 2: 20,891,948 (GRCm39) V161A probably damaging Het
Arhgef18 T C 8: 3,500,317 (GRCm39) I541T possibly damaging Het
Arhgef40 T C 14: 52,227,628 (GRCm39) V458A possibly damaging Het
Atg14 T C 14: 47,788,802 (GRCm39) K184E probably benign Het
Casp8 T C 1: 58,883,929 (GRCm39) V432A probably damaging Het
Ccar2 T C 14: 70,388,540 (GRCm39) T169A possibly damaging Het
Cfap58 G A 19: 47,963,994 (GRCm39) V541M possibly damaging Het
Coro2b T C 9: 62,333,467 (GRCm39) E376G possibly damaging Het
Dnah11 T C 12: 117,880,526 (GRCm39) T3865A probably damaging Het
Dync2h1 T C 9: 7,134,360 (GRCm39) E1549G probably benign Het
Ercc6l2 T C 13: 64,019,982 (GRCm39) probably benign Het
Fbxo32 G T 15: 58,055,729 (GRCm39) T135K possibly damaging Het
Fhip2b A C 14: 70,824,318 (GRCm39) V473G probably damaging Het
Fras1 A T 5: 96,862,235 (GRCm39) I2205F possibly damaging Het
Gm12034 A G 11: 20,396,785 (GRCm39) noncoding transcript Het
Gm26678 T C 3: 54,540,504 (GRCm39) noncoding transcript Het
Gm5108 C A 5: 68,132,496 (GRCm39) T55K unknown Het
Gpa33 A T 1: 165,974,340 (GRCm39) D59V probably damaging Het
Gpn2 T A 4: 133,312,016 (GRCm39) Y83N probably damaging Het
Gzf1 C T 2: 148,528,453 (GRCm39) P460S possibly damaging Het
Ifi203 A T 1: 173,764,099 (GRCm39) S124R possibly damaging Het
Impg1 A G 9: 80,252,611 (GRCm39) L523P probably damaging Het
Ldlrap1 T A 4: 134,484,649 (GRCm39) probably null Het
Madd T C 2: 91,008,581 (GRCm39) E107G probably benign Het
Med12l T G 3: 59,164,644 (GRCm39) probably null Het
Nxpe4 A T 9: 48,310,137 (GRCm39) T467S probably damaging Het
Or52n4 A G 7: 104,294,195 (GRCm39) V126A probably benign Het
Or7g30 T A 9: 19,352,886 (GRCm39) L226I probably damaging Het
Pam A T 1: 97,792,119 (GRCm39) V434D possibly damaging Het
Plxnd1 T C 6: 115,942,914 (GRCm39) N1257D probably benign Het
Prkdc C T 16: 15,653,783 (GRCm39) A3870V probably benign Het
Ralgapa1 C T 12: 55,687,429 (GRCm39) R2019Q probably damaging Het
Ryr2 T C 13: 11,765,611 (GRCm39) D1375G probably benign Het
Skint11 C A 4: 114,101,856 (GRCm39) Q99K probably benign Het
Slc13a2 A G 11: 78,294,361 (GRCm39) probably benign Het
Slc7a13 T C 4: 19,819,070 (GRCm39) F90S probably damaging Het
Spc24 T C 9: 21,667,498 (GRCm39) probably null Het
Stard13 A G 5: 150,986,164 (GRCm39) S331P probably benign Het
Sult2a8 T A 7: 14,147,608 (GRCm39) I228L probably benign Het
Tas2r140 A T 6: 133,031,915 (GRCm39) V281D probably damaging Het
Tmem38b T C 4: 53,840,710 (GRCm39) C66R probably damaging Het
Tshz1 C A 18: 84,034,320 (GRCm39) E29D probably benign Het
Zfp599 T A 9: 22,161,041 (GRCm39) K375* probably null Het
Other mutations in Rufy4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01617:Rufy4 APN 1 74,168,513 (GRCm39) missense probably damaging 1.00
IGL02075:Rufy4 APN 1 74,168,518 (GRCm39) missense probably damaging 1.00
IGL02604:Rufy4 APN 1 74,173,348 (GRCm39) missense probably damaging 1.00
IGL02606:Rufy4 APN 1 74,172,509 (GRCm39) splice site probably benign
IGL02928:Rufy4 APN 1 74,168,241 (GRCm39) unclassified probably benign
R0091:Rufy4 UTSW 1 74,168,095 (GRCm39) unclassified probably benign
R0507:Rufy4 UTSW 1 74,185,875 (GRCm39) missense probably benign 0.02
R0589:Rufy4 UTSW 1 74,172,042 (GRCm39) missense probably damaging 1.00
R0595:Rufy4 UTSW 1 74,180,089 (GRCm39) missense possibly damaging 0.94
R0742:Rufy4 UTSW 1 74,185,875 (GRCm39) missense probably benign 0.02
R1533:Rufy4 UTSW 1 74,169,002 (GRCm39) critical splice donor site probably null
R1666:Rufy4 UTSW 1 74,186,837 (GRCm39) missense probably benign 0.06
R1668:Rufy4 UTSW 1 74,186,837 (GRCm39) missense probably benign 0.06
R1827:Rufy4 UTSW 1 74,173,279 (GRCm39) missense probably damaging 1.00
R2018:Rufy4 UTSW 1 74,180,106 (GRCm39) missense possibly damaging 0.49
R2095:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R2306:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R2307:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R2472:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R2475:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3022:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3054:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3055:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3056:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3117:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3118:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3236:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3237:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3545:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3546:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3547:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3548:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3767:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3768:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3770:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3816:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3817:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3818:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3819:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R3895:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4050:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4091:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4117:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4124:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4125:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4127:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4231:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4234:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4236:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4254:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4255:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4319:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4320:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4321:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4321:Rufy4 UTSW 1 74,171,943 (GRCm39) missense possibly damaging 0.93
R4322:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4323:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4324:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4360:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4361:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4406:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4408:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4516:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4517:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4520:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4522:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4524:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4531:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4533:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4617:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4661:Rufy4 UTSW 1 74,172,266 (GRCm39) missense probably damaging 0.99
R4778:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4779:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4840:Rufy4 UTSW 1 74,168,198 (GRCm39) missense possibly damaging 0.82
R4897:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4898:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4899:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4915:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4917:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4918:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R4997:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5092:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5097:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5189:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5191:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5195:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5196:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5197:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5226:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5227:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5228:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5230:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5372:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5373:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5374:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5375:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5376:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5377:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5378:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5699:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5748:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5750:Rufy4 UTSW 1 74,172,068 (GRCm39) missense probably benign 0.01
R5767:Rufy4 UTSW 1 74,186,822 (GRCm39) missense probably damaging 0.99
R5865:Rufy4 UTSW 1 74,185,914 (GRCm39) missense probably damaging 0.99
R6083:Rufy4 UTSW 1 74,168,556 (GRCm39) missense probably damaging 0.99
R6149:Rufy4 UTSW 1 74,186,892 (GRCm39) missense probably benign 0.15
R6279:Rufy4 UTSW 1 74,172,383 (GRCm39) missense probably benign 0.00
R6300:Rufy4 UTSW 1 74,172,383 (GRCm39) missense probably benign 0.00
R6629:Rufy4 UTSW 1 74,171,526 (GRCm39) splice site probably null
R6809:Rufy4 UTSW 1 74,172,206 (GRCm39) missense probably benign 0.00
R7179:Rufy4 UTSW 1 74,172,035 (GRCm39) missense probably benign 0.12
R7218:Rufy4 UTSW 1 74,172,174 (GRCm39) missense probably damaging 0.99
R7453:Rufy4 UTSW 1 74,168,493 (GRCm39) splice site probably null
R9377:Rufy4 UTSW 1 74,171,879 (GRCm39) missense probably benign 0.08
X0023:Rufy4 UTSW 1 74,180,208 (GRCm39) missense probably benign 0.04
X0025:Rufy4 UTSW 1 74,172,178 (GRCm39) missense probably benign
Predicted Primers PCR Primer
(F):5'- AAGCCATCAGGAGAAGCTGC -3'
(R):5'- AGCTAGGCCTATCTGTGGACTG -3'

Sequencing Primer
(F):5'- GGAGAAGCTGCCACCTCAATTAG -3'
(R):5'- TGGACTGCCAGCTGTTCC -3'
Posted On 2015-06-12