Mutagenetix > Incidental Mutations

Incidental Mutation 'R4235:Tmem266'

321072

Institutional Source

Beutler Lab

Gene Symbol

Tmem266

Ensembl Gene

ENSMUSG00000032313

Gene Name

transmembrane protein 266

Synonyms

AI118078

MMRRC Submission

041052-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R4235 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

55326913-55438345 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 55418107 bp

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 186 (I186T)

Ref Sequence

ENSEMBL: ENSMUSP00000082906 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034862] [ENSMUST00000085754]

Predicted Effect

probably damaging
Transcript: ENSMUST00000034862
AA Change: I251T

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000034862
Gene: ENSMUSG00000032313
AA Change: I251T

Domain	Start	End	E-Value	Type
low complexity region	16	27	N/A	INTRINSIC
transmembrane domain	95	117	N/A	INTRINSIC
transmembrane domain	137	159	N/A	INTRINSIC
transmembrane domain	172	191	N/A	INTRINSIC
low complexity region	260	283	N/A	INTRINSIC
low complexity region	298	309	N/A	INTRINSIC
low complexity region	381	396	N/A	INTRINSIC
low complexity region	423	439	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000085754
AA Change: I186T

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000082906
Gene: ENSMUSG00000032313
AA Change: I186T

Domain	Start	End	E-Value	Type
transmembrane domain	30	52	N/A	INTRINSIC
transmembrane domain	72	94	N/A	INTRINSIC
low complexity region	195	218	N/A	INTRINSIC
low complexity region	233	244	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130465

Meta Mutation Damage Score

0.6129

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.5%

Validation Efficiency

97% (68/70)

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930567H17Rik	C	T	X: 70,394,529	A53T	probably benign	Het
5730596B20Rik	G	T	6: 52,178,961		probably benign	Het
9530053A07Rik	T	A	7: 28,156,648	D1953E	probably damaging	Het
Akap6	T	A	12: 53,139,671	N1289K	probably damaging	Het
Arhgap21	A	G	2: 20,887,137	V161A	probably damaging	Het
Arhgef18	T	C	8: 3,450,317	I541T	possibly damaging	Het
Atp7b	A	G	8: 22,011,023	Y955H	possibly damaging	Het
Bnc2	A	G	4: 84,293,514	V231A	probably damaging	Het
Bod1l	A	G	5: 41,821,455	S839P	probably damaging	Het
Casp8	T	A	1: 58,833,698	H264Q	possibly damaging	Het
Cc2d1b	A	T	4: 108,625,352		probably benign	Het
Cpne6	A	T	14: 55,513,600		probably benign	Het
Ddx1	C	T	12: 13,223,857	V590I	possibly damaging	Het
Dgkd	T	A	1: 87,931,982	L774*	probably null	Het
Fbxl2	T	A	9: 113,989,163	N205I	probably benign	Het
Fkbp15	A	T	4: 62,336,456	I269K	probably benign	Het
Gm13023	G	C	4: 143,794,774	C320S	probably damaging	Het
Gm26678	T	C	3: 54,633,083		noncoding transcript	Het
Has1	G	T	17: 17,850,036	R208S	possibly damaging	Het
Hecw1	C	G	13: 14,317,139	A423P	probably benign	Het
Hspa12b	T	C	2: 131,139,012	V162A	probably benign	Het
Ifi208	T	G	1: 173,682,911	S211A	probably benign	Het
Ighv6-3	T	C	12: 114,391,874	E65G	probably damaging	Het
Igkv9-120	A	T	6: 68,050,333	D77V	probably benign	Het
Impg1	A	G	9: 80,345,329	L523P	probably damaging	Het
Ip6k2	G	A	9: 108,805,648	R319Q	probably benign	Het
Kdm2a	A	G	19: 4,322,521	I932T	probably damaging	Het
Krt17	T	C	11: 100,257,868	T279A	possibly damaging	Het
Lamp1	T	C	8: 13,167,192	V67A	possibly damaging	Het
Limk1	T	C	5: 134,670,478	I142V	probably benign	Het
Mamdc2	T	C	19: 23,374,017	N182D	possibly damaging	Het
Mcpt1	G	A	14: 56,018,560		probably null	Het
Med12l	T	G	3: 59,257,223		probably null	Het
Mfsd10	G	T	5: 34,635,625	T44N	probably damaging	Het
Mrps27	T	C	13: 99,405,041	S218P	probably damaging	Het
Mrps30	T	C	13: 118,386,840	D132G	probably damaging	Het
Neil2	A	C	14: 63,191,841	M1R	probably null	Het
Nelfcd	T	C	2: 174,427,048	F587L	probably damaging	Het
Nfil3	T	C	13: 52,968,799	D23G	probably benign	Het
Nit2	T	C	16: 57,157,160	K169R	probably benign	Het
Nxt1	T	C	2: 148,675,347	S3P	probably benign	Het
Ogt	A	G	X: 101,667,525	N434D	probably damaging	Het
Olfr679	T	A	7: 105,085,787	S24T	possibly damaging	Het
Pcdhga5	A	G	18: 37,695,948	D483G	possibly damaging	Het
Ralgapa1	C	T	12: 55,640,644	R2019Q	probably damaging	Het
Rsl1	T	C	13: 67,177,162		probably null	Het
Sobp	G	A	10: 43,022,900	H230Y	probably damaging	Het
Sptan1	A	G	2: 30,026,588	E2096G	probably damaging	Het
Tie1	G	T	4: 118,478,405	S797*	probably null	Het
Tmem38b	T	C	4: 53,840,710	C66R	probably damaging	Het
Tnfaip6	T	C	2: 52,050,864	F139S	probably damaging	Het
Tnrc6a	T	C	7: 123,171,680	S898P	probably benign	Het
Trim24	A	G	6: 37,964,740	D911G	probably damaging	Het
Tyw5	T	C	1: 57,388,488		probably benign	Het
Ubr3	C	T	2: 70,016,385	Q1651*	probably null	Het
Unc13c	T	A	9: 73,530,952	I1943F	possibly damaging	Het
Usp47	T	A	7: 112,110,048	S1334T	probably damaging	Het
Vmn1r215	T	A	13: 23,075,931	V47E	probably benign	Het
Vmn1r224	T	A	17: 20,419,362	M67K	possibly damaging	Het
Wdfy3	T	A	5: 101,922,634		probably null	Het
Zfc3h1	T	C	10: 115,418,799	Y1433H	probably benign	Het

Other mutations in Tmem266

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01726:Tmem266	APN	9	55435202	missense	probably benign	0.12
IGL03366:Tmem266	APN	9	55437233	missense	probably benign	0.06
R0088:Tmem266	UTSW	9	55437329	missense	probably damaging	1.00
R0418:Tmem266	UTSW	9	55437413	missense	probably benign	0.06
R0497:Tmem266	UTSW	9	55380884	splice site	probably null
R0763:Tmem266	UTSW	9	55414955	missense	probably damaging	1.00
R1657:Tmem266	UTSW	9	55418008	missense	probably damaging	1.00
R3617:Tmem266	UTSW	9	55400634	missense	probably damaging	0.99
R4767:Tmem266	UTSW	9	55380741	missense	probably damaging	1.00
R5587:Tmem266	UTSW	9	55437566	missense	probably damaging	1.00
R5732:Tmem266	UTSW	9	55380836	missense	probably damaging	1.00
R5783:Tmem266	UTSW	9	55397803	missense	probably damaging	1.00
R6193:Tmem266	UTSW	9	55437209	missense	probably benign	0.01
R6943:Tmem266	UTSW	9	55377567	start gained	probably benign
R7459:Tmem266	UTSW	9	55396599	missense	unknown
X0063:Tmem266	UTSW	9	55380749	missense	probably benign	0.00
Z1177:Tmem266	UTSW	9	55397767	missense	probably damaging	1.00
Z1177:Tmem266	UTSW	9	55437470	missense	possibly damaging	0.70

Predicted Primers

PCR Primer
(F):5'- ATGCCCACAAACTGAGTCTGG -3'
(R):5'- ACTGAGCTCTGCAGTGAGTG -3'

Sequencing Primer
(F):5'- CCACAAACTGAGTCTGGGCTTTG -3'
(R):5'- CTCTGCAGTGAGTGGGACATAG -3'

Posted On

2015-06-12