Mutagenetix > Incidental Mutation

Incidental Mutation 'R4235:Vmn1r215'

321084

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r215

Ensembl Gene

ENSMUSG00000099917

Gene Name

vomeronasal 1 receptor 215

Synonyms

V1ri2

MMRRC Submission

041052-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R4235 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

23259962-23260864 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 23260101 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 47 (V47E)

Ref Sequence

ENSEMBL: ENSMUSP00000154763 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072972] [ENSMUST00000228092]

AlphaFold

Q8R264

Predicted Effect

probably benign
Transcript: ENSMUST00000072972
AA Change: V47E

PolyPhen 2 Score 0.314 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000072739
Gene: ENSMUSG00000099917
AA Change: V47E

Domain	Start	End	E-Value	Type
Pfam:V1R	35	298	2.4e-37	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000228092
AA Change: V47E

PolyPhen 2 Score 0.314 (Sensitivity: 0.90; Specificity: 0.89)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.5%

Validation Efficiency

97% (68/70)

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930567H17Rik	C	T	X: 69,438,135 (GRCm39)	A53T	probably benign	Het
5730596B20Rik	G	T	6: 52,155,941 (GRCm39)		probably benign	Het
Akap6	T	A	12: 53,186,454 (GRCm39)	N1289K	probably damaging	Het
Arhgap21	A	G	2: 20,891,948 (GRCm39)	V161A	probably damaging	Het
Arhgef18	T	C	8: 3,500,317 (GRCm39)	I541T	possibly damaging	Het
Atp7b	A	G	8: 22,501,039 (GRCm39)	Y955H	possibly damaging	Het
Bnc2	A	G	4: 84,211,751 (GRCm39)	V231A	probably damaging	Het
Bod1l	A	G	5: 41,978,798 (GRCm39)	S839P	probably damaging	Het
Casp8	T	A	1: 58,872,857 (GRCm39)	H264Q	possibly damaging	Het
Cc2d1b	A	T	4: 108,482,549 (GRCm39)		probably benign	Het
Cpne6	A	T	14: 55,751,057 (GRCm39)		probably benign	Het
Ddx1	C	T	12: 13,273,858 (GRCm39)	V590I	possibly damaging	Het
Dgkd	T	A	1: 87,859,704 (GRCm39)	L774*	probably null	Het
Fbxl2	T	A	9: 113,818,231 (GRCm39)	N205I	probably benign	Het
Fcgbpl1	T	A	7: 27,856,073 (GRCm39)	D1953E	probably damaging	Het
Fkbp15	A	T	4: 62,254,693 (GRCm39)	I269K	probably benign	Het
Gm26678	T	C	3: 54,540,504 (GRCm39)		noncoding transcript	Het
Has1	G	T	17: 18,070,298 (GRCm39)	R208S	possibly damaging	Het
Hecw1	C	G	13: 14,491,724 (GRCm39)	A423P	probably benign	Het
Hspa12b	T	C	2: 130,980,932 (GRCm39)	V162A	probably benign	Het
Ifi208	T	G	1: 173,510,477 (GRCm39)	S211A	probably benign	Het
Ighv6-3	T	C	12: 114,355,494 (GRCm39)	E65G	probably damaging	Het
Igkv9-120	A	T	6: 68,027,317 (GRCm39)	D77V	probably benign	Het
Impg1	A	G	9: 80,252,611 (GRCm39)	L523P	probably damaging	Het
Ip6k2	G	A	9: 108,682,847 (GRCm39)	R319Q	probably benign	Het
Kdm2a	A	G	19: 4,372,549 (GRCm39)	I932T	probably damaging	Het
Krt17	T	C	11: 100,148,694 (GRCm39)	T279A	possibly damaging	Het
Lamp1	T	C	8: 13,217,192 (GRCm39)	V67A	possibly damaging	Het
Limk1	T	C	5: 134,699,332 (GRCm39)	I142V	probably benign	Het
Mamdc2	T	C	19: 23,351,381 (GRCm39)	N182D	possibly damaging	Het
Mcpt1	G	A	14: 56,256,017 (GRCm39)		probably null	Het
Med12l	T	G	3: 59,164,644 (GRCm39)		probably null	Het
Mfsd10	G	T	5: 34,792,969 (GRCm39)	T44N	probably damaging	Het
Mrps27	T	C	13: 99,541,549 (GRCm39)	S218P	probably damaging	Het
Mrps30	T	C	13: 118,523,376 (GRCm39)	D132G	probably damaging	Het
Neil2	A	C	14: 63,429,290 (GRCm39)	M1R	probably null	Het
Nelfcd	T	C	2: 174,268,841 (GRCm39)	F587L	probably damaging	Het
Nfil3	T	C	13: 53,122,835 (GRCm39)	D23G	probably benign	Het
Nit2	T	C	16: 56,977,523 (GRCm39)	K169R	probably benign	Het
Nxt1	T	C	2: 148,517,267 (GRCm39)	S3P	probably benign	Het
Ogt	A	G	X: 100,711,131 (GRCm39)	N434D	probably damaging	Het
Or56a3	T	A	7: 104,734,994 (GRCm39)	S24T	possibly damaging	Het
Pcdhga5	A	G	18: 37,829,001 (GRCm39)	D483G	possibly damaging	Het
Pramel25	G	C	4: 143,521,344 (GRCm39)	C320S	probably damaging	Het
Ralgapa1	C	T	12: 55,687,429 (GRCm39)	R2019Q	probably damaging	Het
Rsl1	T	C	13: 67,325,226 (GRCm39)		probably null	Het
Sobp	G	A	10: 42,898,896 (GRCm39)	H230Y	probably damaging	Het
Sptan1	A	G	2: 29,916,600 (GRCm39)	E2096G	probably damaging	Het
Tie1	G	T	4: 118,335,602 (GRCm39)	S797*	probably null	Het
Tmem266	T	C	9: 55,325,391 (GRCm39)	I186T	probably damaging	Het
Tmem38b	T	C	4: 53,840,710 (GRCm39)	C66R	probably damaging	Het
Tnfaip6	T	C	2: 51,940,876 (GRCm39)	F139S	probably damaging	Het
Tnrc6a	T	C	7: 122,770,903 (GRCm39)	S898P	probably benign	Het
Trim24	A	G	6: 37,941,675 (GRCm39)	D911G	probably damaging	Het
Tyw5	T	C	1: 57,427,647 (GRCm39)		probably benign	Het
Ubr3	C	T	2: 69,846,729 (GRCm39)	Q1651*	probably null	Het
Unc13c	T	A	9: 73,438,234 (GRCm39)	I1943F	possibly damaging	Het
Usp47	T	A	7: 111,709,255 (GRCm39)	S1334T	probably damaging	Het
Vmn1r224	T	A	17: 20,639,624 (GRCm39)	M67K	possibly damaging	Het
Wdfy3	T	A	5: 102,070,500 (GRCm39)		probably null	Het
Zfc3h1	T	C	10: 115,254,704 (GRCm39)	Y1433H	probably benign	Het

Other mutations in Vmn1r215

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00923:Vmn1r215	APN	13	23,260,419 (GRCm39)	missense	probably damaging	0.98
IGL02515:Vmn1r215	APN	13	23,259,990 (GRCm39)	missense	probably benign	0.21
IGL03061:Vmn1r215	APN	13	23,260,088 (GRCm39)	missense	probably damaging	0.97
PIT4651001:Vmn1r215	UTSW	13	23,260,530 (GRCm39)	missense	probably damaging	0.97
R0196:Vmn1r215	UTSW	13	23,260,254 (GRCm39)	missense	probably damaging	1.00
R0816:Vmn1r215	UTSW	13	23,260,124 (GRCm39)	missense	probably benign	0.00
R0817:Vmn1r215	UTSW	13	23,260,124 (GRCm39)	missense	probably benign	0.00
R0820:Vmn1r215	UTSW	13	23,260,124 (GRCm39)	missense	probably benign	0.00
R1104:Vmn1r215	UTSW	13	23,260,758 (GRCm39)	missense	possibly damaging	0.50
R1733:Vmn1r215	UTSW	13	23,260,848 (GRCm39)	missense	probably benign	0.43
R1912:Vmn1r215	UTSW	13	23,260,673 (GRCm39)	missense	possibly damaging	0.94
R2010:Vmn1r215	UTSW	13	23,260,378 (GRCm39)	missense	probably damaging	1.00
R2510:Vmn1r215	UTSW	13	23,260,343 (GRCm39)	missense	probably benign	0.00
R2511:Vmn1r215	UTSW	13	23,260,343 (GRCm39)	missense	probably benign	0.00
R2877:Vmn1r215	UTSW	13	23,260,731 (GRCm39)	missense	probably benign	0.01
R3429:Vmn1r215	UTSW	13	23,260,378 (GRCm39)	missense	probably damaging	1.00
R3854:Vmn1r215	UTSW	13	23,260,058 (GRCm39)	missense	probably benign
R4124:Vmn1r215	UTSW	13	23,260,163 (GRCm39)	missense	probably benign	0.42
R4979:Vmn1r215	UTSW	13	23,260,064 (GRCm39)	missense	probably benign	0.04
R4991:Vmn1r215	UTSW	13	23,260,697 (GRCm39)	missense	probably damaging	1.00
R5011:Vmn1r215	UTSW	13	23,260,721 (GRCm39)	missense	probably damaging	1.00
R5026:Vmn1r215	UTSW	13	23,260,449 (GRCm39)	missense	probably benign	0.30
R5070:Vmn1r215	UTSW	13	23,260,666 (GRCm39)	missense	probably benign	0.03
R5589:Vmn1r215	UTSW	13	23,260,190 (GRCm39)	missense	probably damaging	1.00
R5589:Vmn1r215	UTSW	13	23,260,189 (GRCm39)	missense	probably damaging	1.00
R5651:Vmn1r215	UTSW	13	23,259,981 (GRCm39)	missense	possibly damaging	0.93
R5928:Vmn1r215	UTSW	13	23,260,487 (GRCm39)	missense	possibly damaging	0.50
R6176:Vmn1r215	UTSW	13	23,260,528 (GRCm39)	missense	probably damaging	1.00
R6228:Vmn1r215	UTSW	13	23,260,633 (GRCm39)	missense	probably benign	0.19
R7270:Vmn1r215	UTSW	13	23,260,089 (GRCm39)	missense	possibly damaging	0.77
R7313:Vmn1r215	UTSW	13	23,260,484 (GRCm39)	missense	probably benign	0.01
R7820:Vmn1r215	UTSW	13	23,260,715 (GRCm39)	missense	probably damaging	1.00
R8492:Vmn1r215	UTSW	13	23,260,056 (GRCm39)	missense	possibly damaging	0.79
R8515:Vmn1r215	UTSW	13	23,260,037 (GRCm39)	missense	probably benign	0.00
R8835:Vmn1r215	UTSW	13	23,260,409 (GRCm39)	missense	possibly damaging	0.68
R9428:Vmn1r215	UTSW	13	23,260,749 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TGTGACCAGGCATCCTCAAAG -3'
(R):5'- TGATAGCCTGGACCACACTGAG -3'

Sequencing Primer
(F):5'- CGTGTGTTTCTCAAAAGATTTTTCC -3'
(R):5'- CTGGACCACACTGAGGAGAC -3'

Posted On

2015-06-12