Mutagenetix > Incidental Mutation

Incidental Mutation 'R4163:Zfp831'

321596

Institutional Source

Beutler Lab

Gene Symbol

Zfp831

Ensembl Gene

ENSMUSG00000050600

Gene Name

zinc finger protein 831

Synonyms

ENSMUSG00000050600, OTTMUSG00000017459

MMRRC Submission

041006-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4163 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

174485327-174552625 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 174485822 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 166 (T166A)

Ref Sequence

ENSEMBL: ENSMUSP00000060255 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059452]

AlphaFold

A2ADM8

Predicted Effect

possibly damaging
Transcript: ENSMUST00000059452
AA Change: T166A

PolyPhen 2 Score 0.528 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000060255
Gene: ENSMUSG00000050600
AA Change: T166A

Domain	Start	End	E-Value	Type
low complexity region	120	135	N/A	INTRINSIC
ZnF_C2H2	143	165	5.06e-2	SMART
ZnF_C2H2	171	195	7.78e-3	SMART
low complexity region	201	216	N/A	INTRINSIC
low complexity region	237	248	N/A	INTRINSIC
low complexity region	345	371	N/A	INTRINSIC
low complexity region	383	392	N/A	INTRINSIC
low complexity region	447	459	N/A	INTRINSIC
low complexity region	645	657	N/A	INTRINSIC
low complexity region	717	736	N/A	INTRINSIC
low complexity region	856	870	N/A	INTRINSIC
low complexity region	1520	1529	N/A	INTRINSIC

Meta Mutation Damage Score

0.0965

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

100% (59/59)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930555F03Rik	G	A	8: 49,948,531 (GRCm39)		noncoding transcript	Het
Abca8a	C	T	11: 109,941,808 (GRCm39)	D1154N	probably benign	Het
Adgrf4	A	G	17: 42,978,477 (GRCm39)	F289L	probably benign	Het
Alkbh2	A	G	5: 114,265,613 (GRCm39)	L63S	probably damaging	Het
Atp13a4	A	T	16: 29,360,068 (GRCm39)	N19K	possibly damaging	Het
Birc6	T	A	17: 74,933,975 (GRCm39)	Y2544N	probably damaging	Het
Bnip5	T	C	17: 29,118,503 (GRCm39)	Y643C	probably benign	Het
Bpifa3	A	G	2: 153,977,516 (GRCm39)	N118S	probably damaging	Het
Chst10	T	A	1: 38,910,904 (GRCm39)	M87L	probably benign	Het
Cnot9	C	T	1: 74,568,006 (GRCm39)	P298L	probably damaging	Het
Cops5	T	C	1: 10,100,912 (GRCm39)	Y228C	probably damaging	Het
Dcaf8	T	G	1: 172,020,137 (GRCm39)	L492R	probably damaging	Het
Dis3l2	A	G	1: 86,748,959 (GRCm39)	S128G	probably benign	Het
Dock1	A	G	7: 134,346,051 (GRCm39)	R198G	possibly damaging	Het
Elovl7	T	A	13: 108,403,904 (GRCm39)	M82K	possibly damaging	Het
Entrep1	G	A	19: 23,952,993 (GRCm39)	A439V	probably damaging	Het
Entrep1	C	T	19: 23,953,002 (GRCm39)	S436N	probably damaging	Het
Fcgr2b	T	C	1: 170,791,016 (GRCm39)	D259G	possibly damaging	Het
Flnb	A	T	14: 7,915,374 (GRCm38)	I1502F	possibly damaging	Het
Frk	C	T	10: 34,467,868 (GRCm39)	P294L	probably damaging	Het
Gkn3	C	T	6: 87,360,507 (GRCm39)	A163T	probably damaging	Het
Gm128	C	A	3: 95,147,802 (GRCm39)	R164L	probably benign	Het
Gm2223	C	T	X: 32,943,247 (GRCm39)		noncoding transcript	Het
Hexd	C	A	11: 121,111,975 (GRCm39)	A423E	probably benign	Het
Itgax	G	T	7: 127,743,872 (GRCm39)	V878F	probably benign	Het
Kank2	A	T	9: 21,706,864 (GRCm39)	D51E	probably damaging	Het
Kcna4	G	C	2: 107,126,151 (GRCm39)	W295S	probably damaging	Het
Mfsd12	A	G	10: 81,196,931 (GRCm39)		probably null	Het
Mis12	A	G	11: 70,916,482 (GRCm39)	T172A	probably benign	Het
Myo10	T	A	15: 25,726,501 (GRCm39)		probably null	Het
Myo18a	G	T	11: 77,720,534 (GRCm39)	R1103L	possibly damaging	Het
Nfix	CAAAAA	CAAAA	8: 85,442,876 (GRCm39)		probably null	Het
Ntrk2	T	C	13: 59,008,054 (GRCm39)	V272A	probably damaging	Het
Or2ag17	A	T	7: 106,389,486 (GRCm39)	C241S	probably damaging	Het
Or5p64	A	T	7: 107,855,039 (GRCm39)	I102N	probably benign	Het
Or7e165	A	T	9: 19,695,086 (GRCm39)	Y219F	possibly damaging	Het
Pcdh20	C	T	14: 88,705,615 (GRCm39)	D562N	probably damaging	Het
Prep	T	C	10: 44,943,436 (GRCm39)	F4L	probably benign	Het
Prep	G	T	10: 44,943,458 (GRCm39)	R11L	possibly damaging	Het
Ptgs1	C	T	2: 36,141,346 (GRCm39)	R598C	possibly damaging	Het
Rasgrp1	T	C	2: 117,113,135 (GRCm39)	D759G	probably benign	Het
Setd5	T	C	6: 113,096,545 (GRCm39)	S584P	probably benign	Het
Shank3	A	G	15: 89,433,797 (GRCm39)	D1514G	probably damaging	Het
Shox2	T	C	3: 66,881,104 (GRCm39)		probably benign	Het
Slc44a5	A	T	3: 153,967,010 (GRCm39)	R565S	possibly damaging	Het
Slc7a5	A	G	8: 122,615,139 (GRCm39)	L236S	probably benign	Het
Slfn3	A	T	11: 83,103,596 (GRCm39)	I156F	probably damaging	Het
Spout1	C	T	2: 30,067,589 (GRCm39)		probably benign	Het
Trim72	T	C	7: 127,607,080 (GRCm39)	V203A	probably benign	Het
Vrk2	T	A	11: 26,497,915 (GRCm39)	I90L	probably benign	Het
Zc3h12c	A	T	9: 52,026,999 (GRCm39)	Y788N	probably damaging	Het

Other mutations in Zfp831

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Zfp831	APN	2	174,488,078 (GRCm39)	missense	possibly damaging	0.86
IGL00091:Zfp831	APN	2	174,487,451 (GRCm39)	missense	possibly damaging	0.73
IGL00764:Zfp831	APN	2	174,487,701 (GRCm39)	missense	possibly damaging	0.72
IGL01538:Zfp831	APN	2	174,486,399 (GRCm39)	missense	possibly damaging	0.72
IGL01700:Zfp831	APN	2	174,486,711 (GRCm39)	missense	possibly damaging	0.86
IGL01718:Zfp831	APN	2	174,485,631 (GRCm39)	missense	possibly damaging	0.86
IGL02221:Zfp831	APN	2	174,485,519 (GRCm39)	missense	probably benign	0.33
IGL02250:Zfp831	APN	2	174,489,994 (GRCm39)	missense	possibly damaging	0.53
IGL03209:Zfp831	APN	2	174,487,059 (GRCm39)	missense	probably benign	0.40
D4043:Zfp831	UTSW	2	174,487,059 (GRCm39)	missense	probably benign	0.40
FR4304:Zfp831	UTSW	2	174,487,274 (GRCm39)	small insertion	probably benign
FR4340:Zfp831	UTSW	2	174,487,273 (GRCm39)	small insertion	probably benign
FR4449:Zfp831	UTSW	2	174,487,275 (GRCm39)	small insertion	probably benign
FR4449:Zfp831	UTSW	2	174,487,264 (GRCm39)	small insertion	probably benign
FR4589:Zfp831	UTSW	2	174,487,261 (GRCm39)	small insertion	probably benign
FR4737:Zfp831	UTSW	2	174,487,276 (GRCm39)	small insertion	probably benign
FR4737:Zfp831	UTSW	2	174,487,269 (GRCm39)	small insertion	probably benign
FR4737:Zfp831	UTSW	2	174,487,264 (GRCm39)	small insertion	probably benign
IGL02802:Zfp831	UTSW	2	174,486,945 (GRCm39)	missense	possibly damaging	0.73
P0028:Zfp831	UTSW	2	174,487,139 (GRCm39)	missense	possibly damaging	0.53
PIT4531001:Zfp831	UTSW	2	174,488,516 (GRCm39)	missense	possibly damaging	0.90
R0631:Zfp831	UTSW	2	174,487,083 (GRCm39)	missense	possibly damaging	0.53
R0644:Zfp831	UTSW	2	174,487,656 (GRCm39)	missense	probably benign	0.33
R0782:Zfp831	UTSW	2	174,488,423 (GRCm39)	missense	probably benign	0.06
R1156:Zfp831	UTSW	2	174,488,710 (GRCm39)	missense	possibly damaging	0.53
R1280:Zfp831	UTSW	2	174,545,852 (GRCm39)	missense	probably benign	0.00
R1709:Zfp831	UTSW	2	174,487,683 (GRCm39)	missense	probably benign	0.33
R1883:Zfp831	UTSW	2	174,545,870 (GRCm39)	missense	possibly damaging	0.53
R1884:Zfp831	UTSW	2	174,545,870 (GRCm39)	missense	possibly damaging	0.53
R2127:Zfp831	UTSW	2	174,489,917 (GRCm39)	missense	probably benign	0.33
R2137:Zfp831	UTSW	2	174,547,539 (GRCm39)	missense	possibly damaging	0.53
R2268:Zfp831	UTSW	2	174,486,034 (GRCm39)	missense	probably benign	0.01
R2330:Zfp831	UTSW	2	174,489,882 (GRCm39)	nonsense	probably null
R3547:Zfp831	UTSW	2	174,499,476 (GRCm39)	missense	probably benign
R3821:Zfp831	UTSW	2	174,485,816 (GRCm39)	missense	possibly damaging	0.73
R4232:Zfp831	UTSW	2	174,547,447 (GRCm39)	missense	possibly damaging	0.96
R4778:Zfp831	UTSW	2	174,488,600 (GRCm39)	missense	possibly damaging	0.53
R4820:Zfp831	UTSW	2	174,547,097 (GRCm39)	missense	possibly damaging	0.73
R4912:Zfp831	UTSW	2	174,486,417 (GRCm39)	missense	probably damaging	1.00
R5119:Zfp831	UTSW	2	174,547,103 (GRCm39)	missense	probably benign	0.18
R5152:Zfp831	UTSW	2	174,486,357 (GRCm39)	missense	probably benign	0.33
R5723:Zfp831	UTSW	2	174,487,200 (GRCm39)	missense	probably benign	0.23
R5741:Zfp831	UTSW	2	174,486,945 (GRCm39)	missense	possibly damaging	0.73
R5888:Zfp831	UTSW	2	174,485,420 (GRCm39)	missense	probably benign	0.18
R5975:Zfp831	UTSW	2	174,485,885 (GRCm39)	missense	possibly damaging	0.93
R6092:Zfp831	UTSW	2	174,547,299 (GRCm39)	missense	probably damaging	0.98
R6158:Zfp831	UTSW	2	174,485,651 (GRCm39)	missense	possibly damaging	0.53
R6212:Zfp831	UTSW	2	174,487,661 (GRCm39)	missense	possibly damaging	0.53
R6233:Zfp831	UTSW	2	174,488,490 (GRCm39)	missense	possibly damaging	0.85
R6248:Zfp831	UTSW	2	174,486,308 (GRCm39)	missense	possibly damaging	0.53
R6255:Zfp831	UTSW	2	174,488,214 (GRCm39)	missense	possibly damaging	0.96
R6460:Zfp831	UTSW	2	174,488,360 (GRCm39)	missense	possibly damaging	0.46
R6477:Zfp831	UTSW	2	174,545,960 (GRCm39)	missense	probably benign
R6864:Zfp831	UTSW	2	174,488,533 (GRCm39)	missense	possibly damaging	0.72
R7396:Zfp831	UTSW	2	174,487,002 (GRCm39)	missense	possibly damaging	0.73
R7447:Zfp831	UTSW	2	174,487,896 (GRCm39)	missense	possibly damaging	0.88
R7499:Zfp831	UTSW	2	174,485,816 (GRCm39)	missense	possibly damaging	0.73
R7662:Zfp831	UTSW	2	174,487,934 (GRCm39)	missense	possibly damaging	0.85
R7857:Zfp831	UTSW	2	174,547,035 (GRCm39)	missense	probably benign	0.33
R7889:Zfp831	UTSW	2	174,487,097 (GRCm39)	missense	possibly damaging	0.53
R7896:Zfp831	UTSW	2	174,488,921 (GRCm39)	missense	possibly damaging	0.53
R8074:Zfp831	UTSW	2	174,486,528 (GRCm39)	missense	possibly damaging	0.72
R8089:Zfp831	UTSW	2	174,486,717 (GRCm39)	missense	possibly damaging	0.96
R8438:Zfp831	UTSW	2	174,486,796 (GRCm39)	missense	possibly damaging	0.53
R8716:Zfp831	UTSW	2	174,547,049 (GRCm39)	missense	possibly damaging	0.53
R8757:Zfp831	UTSW	2	174,487,874 (GRCm39)	missense	probably benign
R8759:Zfp831	UTSW	2	174,487,874 (GRCm39)	missense	probably benign
R8899:Zfp831	UTSW	2	174,485,978 (GRCm39)	missense	probably damaging	0.97
R8976:Zfp831	UTSW	2	174,487,079 (GRCm39)	missense	possibly damaging	0.76
R9146:Zfp831	UTSW	2	174,487,461 (GRCm39)	missense	possibly damaging	0.72
R9257:Zfp831	UTSW	2	174,488,156 (GRCm39)	missense	possibly damaging	0.53
R9324:Zfp831	UTSW	2	174,547,113 (GRCm39)	missense	probably benign	0.33
R9467:Zfp831	UTSW	2	174,486,789 (GRCm39)	missense	probably benign	0.33
R9729:Zfp831	UTSW	2	174,487,938 (GRCm39)	missense	possibly damaging	0.96
X0021:Zfp831	UTSW	2	174,547,662 (GRCm39)	missense	possibly damaging	0.85
Z1177:Zfp831	UTSW	2	174,485,981 (GRCm39)	missense	possibly damaging	0.93

Predicted Primers

PCR Primer
(F):5'- CGCCCTGACTGTGAACATTG -3'
(R):5'- CTTTCCCTGTTCTGAGCAGCAAG -3'

Sequencing Primer
(F):5'- CCTGACTGTGAACATTGTAGGCAC -3'
(R):5'- ACTATCCACTGAGGCGGTCTC -3'

Posted On

2015-06-12