Mutagenetix > Incidental Mutation

Incidental Mutation 'R4256:Vmn1r64'

321818

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r64

Ensembl Gene

ENSMUSG00000058399

Gene Name

vomeronasal 1 receptor 64

Synonyms

V1rd11

MMRRC Submission

041069-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R4256 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

5886579-5887541 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 5886895 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 216 (H216L)

Ref Sequence

ENSEMBL: ENSMUSP00000077568 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078475]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000078475
AA Change: H216L

PolyPhen 2 Score 0.046 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000077568
Gene: ENSMUSG00000058399
AA Change: H216L

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	296	3.3e-13	PFAM
Pfam:V1R	41	296	1.5e-19	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.0%

Validation Efficiency

93% (41/44)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930578I06Rik	C	T	14: 64,210,658 (GRCm39)	R190H	probably benign	Het
Arsi	T	C	18: 61,050,388 (GRCm39)	W424R	probably damaging	Het
Atad2	A	G	15: 57,980,252 (GRCm39)	S411P	probably damaging	Het
Cdhr2	G	A	13: 54,861,818 (GRCm39)	V72I	probably damaging	Het
Celf4	T	C	18: 25,624,258 (GRCm39)	I414V	probably damaging	Het
Cfap43	G	A	19: 47,770,844 (GRCm39)	T689I	probably benign	Het
Cpne9	C	T	6: 113,259,984 (GRCm39)		probably benign	Het
Cyp3a11	A	T	5: 145,806,005 (GRCm39)	S121T	probably benign	Het
Dip2c	C	A	13: 9,659,092 (GRCm39)	Q864K	probably damaging	Het
Fbxo3	A	G	2: 103,881,510 (GRCm39)	T281A	probably damaging	Het
Gm5148	T	A	3: 37,768,758 (GRCm39)	H154L	unknown	Het
Gsdma2	T	A	11: 98,542,758 (GRCm39)		probably null	Het
Hfm1	T	C	5: 107,052,663 (GRCm39)	I273M	possibly damaging	Het
Hspa4l	A	G	3: 40,700,435 (GRCm39)	E14G	probably benign	Het
Inava	G	T	1: 136,142,088 (GRCm39)	N670K	probably benign	Het
Lgals12	T	G	19: 7,584,081 (GRCm39)	E5D	possibly damaging	Het
Lsg1	T	G	16: 30,392,061 (GRCm39)	I237L	probably benign	Het
Mettl14	T	C	3: 123,177,254 (GRCm39)	E49G	probably damaging	Het
Nbeal1	A	G	1: 60,370,107 (GRCm39)	I2675V	probably benign	Het
Or2y1e	A	T	11: 49,218,304 (GRCm39)	Q22L	probably benign	Het
Or4b1b	A	T	2: 90,112,406 (GRCm39)	V171E	probably damaging	Het
Or5k15	T	G	16: 58,710,143 (GRCm39)	S147R	probably benign	Het
Padi1	A	T	4: 140,542,089 (GRCm39)	L611Q	probably damaging	Het
Pcdhac2	A	G	18: 37,277,764 (GRCm39)	D248G	probably damaging	Het
Plekhm1	C	A	11: 103,261,760 (GRCm39)	R940L	probably damaging	Het
Rasa3	A	G	8: 13,664,532 (GRCm39)		probably null	Het
Rspo2	C	A	15: 42,939,307 (GRCm39)	R161L	probably benign	Het
Sacs	A	G	14: 61,443,786 (GRCm39)	Y1944C	probably damaging	Het
Slc7a10	G	T	7: 34,898,140 (GRCm39)	M297I	probably damaging	Het
Spata31e3	A	C	13: 50,404,141 (GRCm39)	S54A	probably benign	Het
Ssh2	A	G	11: 77,299,009 (GRCm39)	T112A	possibly damaging	Het
Ttc7	A	T	17: 87,628,829 (GRCm39)		probably null	Het
Vmn2r112	A	G	17: 22,837,393 (GRCm39)	K618R	probably damaging	Het
Vmp1	T	A	11: 86,552,014 (GRCm39)	I117L	probably benign	Het
Vsnl1	A	T	12: 11,382,056 (GRCm39)	Y108*	probably null	Het
Wdr31	A	G	4: 62,375,675 (GRCm39)		probably null	Het
Zfp329	A	G	7: 12,541,840 (GRCm39)	V284A	probably benign	Het
Zfp551	G	A	7: 12,150,318 (GRCm39)	H364Y	possibly damaging	Het

Other mutations in Vmn1r64

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00161:Vmn1r64	APN	7	5,886,827 (GRCm39)	missense	probably damaging	0.98
IGL01070:Vmn1r64	APN	7	5,886,941 (GRCm39)	missense	probably benign	0.04
IGL01817:Vmn1r64	APN	7	5,887,222 (GRCm39)	missense	probably damaging	1.00
IGL02026:Vmn1r64	APN	7	5,886,649 (GRCm39)	missense	possibly damaging	0.84
IGL02657:Vmn1r64	APN	7	5,886,727 (GRCm39)	missense	probably benign	0.33
IGL03352:Vmn1r64	APN	7	5,887,070 (GRCm39)	missense	probably benign	0.12
R0200:Vmn1r64	UTSW	7	5,886,817 (GRCm39)	missense	probably benign	0.38
R0540:Vmn1r64	UTSW	7	5,887,096 (GRCm39)	missense	probably damaging	1.00
R0655:Vmn1r64	UTSW	7	5,887,207 (GRCm39)	missense	probably benign	0.25
R1212:Vmn1r64	UTSW	7	5,887,210 (GRCm39)	missense	probably damaging	1.00
R1579:Vmn1r64	UTSW	7	5,886,803 (GRCm39)	missense	probably damaging	1.00
R2034:Vmn1r64	UTSW	7	5,886,988 (GRCm39)	missense	probably benign	0.09
R2221:Vmn1r64	UTSW	7	5,887,448 (GRCm39)	missense	probably benign	0.00
R2240:Vmn1r64	UTSW	7	5,887,369 (GRCm39)	nonsense	probably null
R2305:Vmn1r64	UTSW	7	5,887,535 (GRCm39)	missense	probably benign	0.04
R3019:Vmn1r64	UTSW	7	5,887,226 (GRCm39)	missense	probably damaging	1.00
R4666:Vmn1r64	UTSW	7	5,887,357 (GRCm39)	missense	probably damaging	0.99
R4996:Vmn1r64	UTSW	7	5,887,052 (GRCm39)	missense	probably benign	0.03
R5682:Vmn1r64	UTSW	7	5,886,622 (GRCm39)	missense	possibly damaging	0.79
R5730:Vmn1r64	UTSW	7	5,887,522 (GRCm39)	missense	probably benign	0.01
R7397:Vmn1r64	UTSW	7	5,887,013 (GRCm39)	missense	possibly damaging	0.87
R9697:Vmn1r64	UTSW	7	5,886,859 (GRCm39)	missense	probably benign	0.04
X0063:Vmn1r64	UTSW	7	5,887,349 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CAGTGGGGAAGCTTAGATGC -3'
(R):5'- TGCTTACATGCCAATGTATGTCAG -3'

Sequencing Primer
(F):5'- GGAAGCTTAGATGCAAAAGTTCTATG -3'
(R):5'- CAATGTATGTCAGTGGTCCACAG -3'

Posted On

2015-06-20