Mutagenetix > Incidental Mutation

Incidental Mutation 'R4283:Tas2r143'

321945

Institutional Source

Beutler Lab

Gene Symbol

Tas2r143

Ensembl Gene

ENSMUSG00000046652

Gene Name

taste receptor, type 2, member 143

Synonyms

Tas2r43, mt2r36

MMRRC Submission

041651-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.048) question?

Stock #

R4283 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

42377172-42378053 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 42378007 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000070247 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057398] [ENSMUST00000070178]

AlphaFold

Q7TQB9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000057398
AA Change: L279Q

PolyPhen 2 Score 0.891 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000057910
Gene: ENSMUSG00000046652
AA Change: L279Q

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	293	6.7e-68	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000070178

SMART Domains

Protein: ENSMUSP00000070247
Gene: ENSMUSG00000056203

Domain	Start	End	E-Value	Type
Pfam:TAS2R	22	320	1.3e-63	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.4%

Validation Efficiency

100% (41/41)

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6030468B19Rik	A	G	11: 117,697,087 (GRCm39)	T229A	probably benign	Het
Adgrg5	T	G	8: 95,664,326 (GRCm39)	V312G	probably benign	Het
Arl6ip1	AAAATAAATAAATAAATAAATAAATA	AAAATAAATAAATAAATAAATAAATAAATA	7: 117,721,122 (GRCm39)		probably benign	Het
Camk2b	A	C	11: 5,937,099 (GRCm39)	S327A	probably benign	Het
Card9	A	G	2: 26,247,309 (GRCm39)	I280T	possibly damaging	Het
Cd274	A	T	19: 29,357,871 (GRCm39)	M188L	probably benign	Het
Cga	T	C	4: 34,905,264 (GRCm39)		probably null	Het
Copg1	C	T	6: 87,885,527 (GRCm39)	T723M	probably damaging	Het
Cplx2	A	G	13: 54,527,377 (GRCm39)	E87G	probably damaging	Het
Crtc2	A	G	3: 90,166,543 (GRCm39)		probably benign	Het
Defa27	A	G	8: 21,805,632 (GRCm39)	N24S	probably benign	Het
Defb40	A	G	8: 19,028,093 (GRCm39)	S14P	probably damaging	Het
Dnmt3a	G	A	12: 3,951,665 (GRCm39)	G681R	probably damaging	Het
Ghr	C	A	15: 3,362,930 (GRCm39)	V181L	possibly damaging	Het
Gm10750	A	G	2: 148,857,916 (GRCm39)	F112L	unknown	Het
Gm17541	A	G	12: 4,739,656 (GRCm39)		probably benign	Het
Gmip	T	A	8: 70,266,251 (GRCm39)		probably benign	Het
Hdac10	A	G	15: 89,009,826 (GRCm39)	L402P	possibly damaging	Het
Hsd17b12	G	C	2: 93,863,931 (GRCm39)	R304G	unknown	Het
Iqgap3	T	C	3: 88,006,167 (GRCm39)	V526A	probably benign	Het
Ncaph	A	G	2: 126,963,005 (GRCm39)		probably benign	Het
Ninl	T	C	2: 150,795,336 (GRCm39)		probably benign	Het
Pcgf1	T	A	6: 83,056,714 (GRCm39)	L90Q	probably damaging	Het
Pcsk4	C	A	10: 80,165,287 (GRCm39)		probably benign	Het
Ptpn21	T	C	12: 98,699,734 (GRCm39)	E60G	probably damaging	Het
Rec8	A	G	14: 55,856,091 (GRCm39)	H11R	probably damaging	Het
Ric1	A	G	19: 29,563,950 (GRCm39)	Y568C	probably damaging	Het
Slc17a6	A	G	7: 51,294,824 (GRCm39)	Y177C	probably damaging	Het
Tas2r104	C	T	6: 131,662,374 (GRCm39)	A112T	probably damaging	Het
Tas2r123	G	A	6: 132,825,008 (GRCm39)	V302I	possibly damaging	Het
Tfeb	A	G	17: 48,100,699 (GRCm39)	E305G	probably damaging	Het
Ttn	T	A	2: 76,585,168 (GRCm39)	I22042F	probably damaging	Het
Upf2	A	G	2: 5,978,369 (GRCm39)	N411S	unknown	Het
Vmn2r52	C	T	7: 9,904,565 (GRCm39)	G425R	possibly damaging	Het
Zfp28	A	T	7: 6,396,700 (GRCm39)	Q378H	probably benign	Het
Zfp429	A	C	13: 67,538,914 (GRCm39)	C177G	probably damaging	Het

Other mutations in Tas2r143

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02216:Tas2r143	APN	6	42,377,268 (GRCm39)	nonsense	probably null
IGL02832:Tas2r143	APN	6	42,377,259 (GRCm39)	missense	possibly damaging	0.55
R0125:Tas2r143	UTSW	6	42,377,889 (GRCm39)	missense	probably benign	0.01
R1035:Tas2r143	UTSW	6	42,377,199 (GRCm39)	missense	probably benign	0.16
R1073:Tas2r143	UTSW	6	42,377,694 (GRCm39)	missense	probably benign	0.01
R1400:Tas2r143	UTSW	6	42,377,317 (GRCm39)	missense	probably benign	0.35
R1774:Tas2r143	UTSW	6	42,377,305 (GRCm39)	missense	probably damaging	1.00
R2391:Tas2r143	UTSW	6	42,377,810 (GRCm39)	missense	probably damaging	0.99
R3617:Tas2r143	UTSW	6	42,377,997 (GRCm39)	missense	probably benign	0.20
R3693:Tas2r143	UTSW	6	42,377,910 (GRCm39)	missense	probably benign	0.00
R4486:Tas2r143	UTSW	6	42,377,628 (GRCm39)	missense	probably benign	0.15
R5005:Tas2r143	UTSW	6	42,377,658 (GRCm39)	missense	probably benign	0.02
R6360:Tas2r143	UTSW	6	42,377,769 (GRCm39)	missense	probably benign	0.40
R7163:Tas2r143	UTSW	6	42,377,202 (GRCm39)	missense	probably benign
R7827:Tas2r143	UTSW	6	42,377,656 (GRCm39)	missense	probably damaging	1.00
R8899:Tas2r143	UTSW	6	42,377,888 (GRCm39)	nonsense	probably null
R8989:Tas2r143	UTSW	6	42,377,828 (GRCm39)	missense	probably damaging	1.00
R9264:Tas2r143	UTSW	6	42,377,673 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- ACTCAGGCACATACCATGGC -3'
(R):5'- TTGGAACTCAGACCAGGAAC -3'

Sequencing Primer
(F):5'- GGCACATACCATGGCTCTGAAATC -3'
(R):5'- CTCAGACCAGGAACTAACATAAATG -3'

Posted On

2015-06-20