Mutagenetix > Incidental Mutation

Incidental Mutation 'R4273:Or10al7'

322303

Institutional Source

Beutler Lab

Gene Symbol

Or10al7

Ensembl Gene

ENSMUSG00000081724

Gene Name

olfactory receptor family 10 subfamily AL member 7

Synonyms

MOR263-9, GA_x6K02T2PSCP-2503741-2502776, Olfr129

MMRRC Submission

041645-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.136) question?

Stock #

R4273 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

38365490-38370675 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 38366163 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 98 (I98N)

Ref Sequence

ENSEMBL: ENSMUSP00000149327 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000122318] [ENSMUST00000174675] [ENSMUST00000216476]

AlphaFold

Q8VEY1

Predicted Effect

probably damaging
Transcript: ENSMUST00000122318
AA Change: I107N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000113564
Gene: ENSMUSG00000081724
AA Change: I107N

Domain	Start	End	E-Value	Type
Pfam:7tm_4	46	323	4.6e-56	PFAM
Pfam:7TM_GPCR_Srsx	50	319	1.1e-5	PFAM
Pfam:7tm_1	56	305	3.2e-21	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000174675
AA Change: I98N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000133865
Gene: ENSMUSG00000060017
AA Change: I98N

Domain	Start	End	E-Value	Type
transmembrane domain	33	55	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000216476
AA Change: I98N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.7%
20x: 96.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acer2	T	A	4: 86,792,835 (GRCm39)		probably null	Het
Adgrf2	T	A	17: 43,021,013 (GRCm39)	T604S	probably damaging	Het
Akap8l	T	A	17: 32,540,905 (GRCm39)	K533*	probably null	Het
Appbp2	T	C	11: 85,125,502 (GRCm39)	Y45C	probably damaging	Het
Arap2	T	C	5: 62,828,322 (GRCm39)	I950V	possibly damaging	Het
Arhgap31	T	C	16: 38,422,697 (GRCm39)	E1123G	possibly damaging	Het
Atp2c1	G	T	9: 105,312,339 (GRCm39)	N493K	probably benign	Het
Bcr	T	C	10: 74,960,943 (GRCm39)	I458T	probably damaging	Het
Brd4	G	A	17: 32,433,756 (GRCm39)	T468I	probably benign	Het
Cdh23	T	A	10: 60,146,940 (GRCm39)	D2774V	possibly damaging	Het
Cfdp1	T	C	8: 112,495,417 (GRCm39)	Y267C	probably damaging	Het
Chd6	C	A	2: 160,803,211 (GRCm39)	A2156S	probably benign	Het
Dazap2	C	A	15: 100,515,971 (GRCm39)	P100T	probably damaging	Het
Disp1	T	A	1: 182,869,208 (GRCm39)	I1071F	possibly damaging	Het
Dlgap1	T	A	17: 71,073,038 (GRCm39)	S686T	probably benign	Het
Dst	C	T	1: 34,231,421 (GRCm39)	R3183C	possibly damaging	Het
Enpp4	T	C	17: 44,412,698 (GRCm39)	N279D	probably benign	Het
Exoc3l	T	C	8: 106,016,593 (GRCm39)	*740W	probably null	Het
Exoc5	A	T	14: 49,252,937 (GRCm39)	C625*	probably null	Het
Fam98b	A	T	2: 117,090,712 (GRCm39)	N137Y	possibly damaging	Het
Fat4	T	A	3: 38,945,776 (GRCm39)	D1556E	probably damaging	Het
Fcer2a	C	A	8: 3,732,848 (GRCm39)	V319L	possibly damaging	Het
Fer1l6	T	C	15: 58,499,371 (GRCm39)	V1247A	probably benign	Het
Fmo4	A	G	1: 162,632,748 (GRCm39)	V201A	probably damaging	Het
Fras1	G	A	5: 96,762,763 (GRCm39)	G755D	probably benign	Het
Grid2	T	C	6: 63,886,029 (GRCm39)	Y142H	probably damaging	Het
Hspg2	C	T	4: 137,246,251 (GRCm39)	R1010C	probably damaging	Het
Ibtk	T	C	9: 85,608,784 (GRCm39)	Q376R	probably damaging	Het
Impdh2	T	C	9: 108,442,155 (GRCm39)	M414T	probably damaging	Het
Itm2c	A	G	1: 85,834,750 (GRCm39)	T160A	probably damaging	Het
Kcna2	T	A	3: 107,012,509 (GRCm39)	D363E	probably benign	Het
Lama2	C	T	10: 27,223,050 (GRCm39)	C412Y	probably damaging	Het
Lims2	C	G	18: 32,089,390 (GRCm39)	T151S	probably benign	Het
Mier1	T	C	4: 103,019,628 (GRCm39)	S423P	possibly damaging	Het
Mrgpra3	A	T	7: 47,239,180 (GRCm39)	W249R	probably benign	Het
Mtor	A	G	4: 148,634,609 (GRCm39)	H2410R	probably benign	Het
Mvp	C	T	7: 126,588,875 (GRCm39)	A631T	probably benign	Het
Nepro	T	C	16: 44,556,192 (GRCm39)	V450A	possibly damaging	Het
Ngrn	T	C	7: 79,914,269 (GRCm39)	V140A	probably damaging	Het
Nobox	T	C	6: 43,282,942 (GRCm39)	E231G	probably benign	Het
P3h2	T	A	16: 25,923,971 (GRCm39)	I155F	probably benign	Het
Pcdha3	C	T	18: 37,081,144 (GRCm39)	R629C	probably damaging	Het
Pramel24	A	T	4: 143,453,416 (GRCm39)	K175*	probably null	Het
Riok3	AGAAGCGG	AG	18: 12,268,998 (GRCm39)		probably benign	Het
Rttn	T	C	18: 89,110,020 (GRCm39)	I1675T	probably benign	Het
Sall2	C	A	14: 52,551,260 (GRCm39)	R643L	probably damaging	Het
Slc35f4	G	T	14: 49,541,758 (GRCm39)	T182N	possibly damaging	Het
Slc52a3	T	A	2: 151,847,660 (GRCm39)	I256N	possibly damaging	Het
Sox9	T	C	11: 112,675,980 (GRCm39)	S390P	possibly damaging	Het
Tango2	T	C	16: 18,120,654 (GRCm39)		probably benign	Het
Tas1r1	T	C	4: 152,116,614 (GRCm39)	E340G	possibly damaging	Het
Tek	G	A	4: 94,718,207 (GRCm39)	G524R	probably damaging	Het
Tmem260	A	T	14: 48,742,761 (GRCm39)	Y532F	probably benign	Het
Tsks	C	A	7: 44,607,353 (GRCm39)	L559I	probably damaging	Het
Unc79	C	A	12: 103,088,612 (GRCm39)	L1702I	probably damaging	Het
Vmn1r14	T	A	6: 57,211,133 (GRCm39)	I237N	probably damaging	Het
Vmn2r17	G	A	5: 109,600,832 (GRCm39)	C710Y	probably benign	Het
Zfp119b	G	T	17: 56,245,926 (GRCm39)	T420K	possibly damaging	Het
Zfp202	C	T	9: 40,118,790 (GRCm39)	R68*	probably null	Het
Zfp229	T	A	17: 21,965,802 (GRCm39)	S677R	probably benign	Het
Zfp462	C	T	4: 55,008,411 (GRCm39)	H126Y	probably benign	Het
Zfp52	C	A	17: 21,780,459 (GRCm39)	Y102*	probably null	Het
Zfp616	T	A	11: 73,974,526 (GRCm39)	M265K	probably benign	Het
Zfyve9	A	T	4: 108,538,173 (GRCm39)	I1031N	probably damaging	Het
Zmynd11	T	C	13: 9,747,726 (GRCm39)	Y203C	probably damaging	Het

Other mutations in Or10al7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02140:Or10al7	APN	17	38,366,481 (GRCm39)	start codon destroyed	probably null	0.15
IGL02163:Or10al7	APN	17	38,365,641 (GRCm39)	missense	probably benign	0.06
IGL02374:Or10al7	APN	17	38,366,412 (GRCm39)	missense	probably damaging	1.00
R0840:Or10al7	UTSW	17	38,366,463 (GRCm39)	missense	probably benign	0.00
R1774:Or10al7	UTSW	17	38,366,328 (GRCm39)	missense	probably benign	0.00
R3720:Or10al7	UTSW	17	38,366,259 (GRCm39)	missense	probably damaging	1.00
R3794:Or10al7	UTSW	17	38,365,786 (GRCm39)	missense	probably damaging	1.00
R3840:Or10al7	UTSW	17	38,366,239 (GRCm39)	missense	probably damaging	1.00
R4002:Or10al7	UTSW	17	38,365,879 (GRCm39)	missense	probably damaging	1.00
R4872:Or10al7	UTSW	17	38,366,467 (GRCm39)	missense	probably benign
R5606:Or10al7	UTSW	17	38,365,693 (GRCm39)	missense	probably damaging	0.98
R6309:Or10al7	UTSW	17	38,366,043 (GRCm39)	missense	probably damaging	1.00
R7269:Or10al7	UTSW	17	38,366,442 (GRCm39)	missense	probably damaging	1.00
R7450:Or10al7	UTSW	17	38,366,000 (GRCm39)	missense	probably benign	0.00
R7829:Or10al7	UTSW	17	38,366,220 (GRCm39)	missense	possibly damaging	0.64
R8103:Or10al7	UTSW	17	38,365,903 (GRCm39)	missense	probably damaging	1.00
R8994:Or10al7	UTSW	17	38,366,220 (GRCm39)	missense	possibly damaging	0.64
R9388:Or10al7	UTSW	17	38,366,148 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACAATGCAACCTGTTCCCCATG -3'
(R):5'- TCCCTGGAGAATGCTTCCTC -3'

Sequencing Primer
(F):5'- CCATGAAACTATTGCCAAATGGG -3'
(R):5'- GGAGAATGCTTCCTCCTGTTTAC -3'

Posted On

2015-06-20