Mutagenetix > Incidental Mutations

Incidental Mutation 'R4334:Zfp810'

323698

Institutional Source

Beutler Lab

Gene Symbol

Zfp810

Ensembl Gene

ENSMUSG00000066829

Gene Name

zinc finger protein 810

Synonyms

MMRRC Submission

041664-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R4334 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

22276748-22307648 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 22278784 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 276 (Y276C)

Ref Sequence

ENSEMBL: ENSMUSP00000083459 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086278] [ENSMUST00000215202]

AlphaFold

Q99K45

Predicted Effect

probably benign
Transcript: ENSMUST00000086278
AA Change: Y276C

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000083459
Gene: ENSMUSG00000066829
AA Change: Y276C

Domain	Start	End	E-Value	Type
KRAB	4	64	1.09e-33	SMART
ZnF_C2H2	126	148	2.44e2	SMART
ZnF_C2H2	182	204	3.07e-1	SMART
ZnF_C2H2	210	232	8.47e-4	SMART
ZnF_C2H2	238	260	6.78e-3	SMART
ZnF_C2H2	266	288	6.13e-1	SMART
ZnF_C2H2	294	316	5.06e-2	SMART
ZnF_C2H2	322	344	4.79e-3	SMART
ZnF_C2H2	350	372	2.99e-4	SMART
ZnF_C2H2	378	400	1.33e-1	SMART
ZnF_C2H2	406	428	2.75e-3	SMART
ZnF_C2H2	434	456	1.58e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214499

Predicted Effect

probably benign
Transcript: ENSMUST00000215202

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	A	T	17: 24,318,268	V447E	probably damaging	Het
Adcyap1	T	C	17: 93,202,268	L49P	probably benign	Het
B4galt4	T	C	16: 38,752,259	I102T	probably damaging	Het
Cc2d2a	A	G	5: 43,683,134	D110G	probably benign	Het
Ccdc141	C	T	2: 77,170,432	V19I	probably damaging	Het
Ccdc39	A	G	3: 33,837,882	L147P	probably damaging	Het
Cdc5l	A	T	17: 45,410,786	D519E	probably benign	Het
Cdh23	G	T	10: 60,385,059	T1305K	probably damaging	Het
Cfi	T	A	3: 129,850,829	V158D	possibly damaging	Het
Clstn2	T	G	9: 97,463,528	Y589S	probably damaging	Het
Dsp	A	G	13: 38,196,664	K1863E	possibly damaging	Het
Enpp3	A	T	10: 24,793,589	M491K	probably damaging	Het
Fhdc1	C	A	3: 84,444,826	V1031F	probably benign	Het
Fkbp15	G	A	4: 62,303,219	A1170V	possibly damaging	Het
Foxa2	T	C	2: 148,044,703	N64S	possibly damaging	Het
Foxm1	T	C	6: 128,365,967	I88T	probably damaging	Het
Gm3173	A	T	14: 4,514,779	I8L	possibly damaging	Het
Gpatch3	A	G	4: 133,582,481	D375G	probably damaging	Het
Herc2	T	C	7: 56,226,654	F4436L	probably damaging	Het
Htr3b	G	A	9: 48,945,509	A223V	probably damaging	Het
Iars2	T	A	1: 185,303,394	T550S	probably benign	Het
Igsf9	A	T	1: 172,494,212	K149*	probably null	Het
Khnyn	A	G	14: 55,894,042	D536G	probably damaging	Het
Kng1	T	C	16: 23,079,620	V409A	possibly damaging	Het
Krt1	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	15: 101,850,378		probably benign	Het
Madd	C	T	2: 91,140,572	V1508I	probably benign	Het
Micall2	T	C	5: 139,713,350	E527G	probably damaging	Het
Myo7b	A	G	18: 31,976,987	S1141P	probably damaging	Het
Ncoa2	G	A	1: 13,174,963	P504S	possibly damaging	Het
Notch1	G	A	2: 26,460,036	T2364I	probably benign	Het
Pde4c	T	A	8: 70,749,826		probably null	Het
Pik3cb	A	G	9: 99,061,851	L633P	probably damaging	Het
Ranbp2	T	A	10: 58,463,994	D483E	probably damaging	Het
Sh3tc2	A	G	18: 61,990,321	N718D	probably damaging	Het
Spast	G	A	17: 74,352,015	A126T	probably damaging	Het
Ssc5d	T	C	7: 4,943,664	S1006P	probably benign	Het
Tmprss6	T	A	15: 78,459,427		probably null	Het
Ulk1	C	T	5: 110,789,357	R691Q	probably benign	Het
Unc79	C	T	12: 103,078,974	T803M	probably benign	Het
Vmn2r118	A	T	17: 55,610,347	F388L	possibly damaging	Het
Vmn2r71	T	C	7: 85,619,834	V415A	probably benign	Het
Wdr4	A	T	17: 31,499,152	F316Y	possibly damaging	Het

Other mutations in Zfp810

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00484:Zfp810	APN	9	22278309	nonsense	probably null
IGL03079:Zfp810	APN	9	22284127	missense	probably damaging	1.00
IGL03402:Zfp810	APN	9	22279145	splice site	probably null
H8562:Zfp810	UTSW	9	22279091	missense	probably benign	0.42
R1116:Zfp810	UTSW	9	22279085	missense	probably benign	0.11
R1160:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R1171:Zfp810	UTSW	9	22278826	missense	possibly damaging	0.95
R1393:Zfp810	UTSW	9	22280514	missense	probably benign
R1608:Zfp810	UTSW	9	22278920	missense	probably benign	0.00
R1644:Zfp810	UTSW	9	22279028	missense	possibly damaging	0.67
R1766:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R2568:Zfp810	UTSW	9	22279238	missense	probably benign	0.01
R3684:Zfp810	UTSW	9	22278235	missense	probably benign	0.01
R4002:Zfp810	UTSW	9	22278892	missense	probably damaging	1.00
R4134:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4135:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4545:Zfp810	UTSW	9	22278745	missense	probably damaging	0.96
R5399:Zfp810	UTSW	9	22278829	missense	possibly damaging	0.91
R5622:Zfp810	UTSW	9	22279096	missense	probably benign	0.00
R5643:Zfp810	UTSW	9	22283171	missense	probably benign	0.26
R7375:Zfp810	UTSW	9	22290537	critical splice donor site	probably null
R7441:Zfp810	UTSW	9	22279272	nonsense	probably null
R7809:Zfp810	UTSW	9	22278982	missense	possibly damaging	0.51
R8422:Zfp810	UTSW	9	22283222	nonsense	probably null
R8526:Zfp810	UTSW	9	22278290	missense	probably damaging	1.00
R8719:Zfp810	UTSW	9	22279275	missense	probably benign	0.00
R9177:Zfp810	UTSW	9	22278640	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AACGGACTTATTAAGTATTACCCTGCT -3'
(R):5'- ACTGCAAGTCTCACCTCAC -3'

Sequencing Primer
(F):5'- ACTCGGGACATTCATATGGC -3'
(R):5'- TGCAGGAAGGCTTTCTACAGC -3'

Posted On

2015-06-24