Mutagenetix > Incidental Mutations

Incidental Mutation 'R4305:Zdhhc4'

323993

Institutional Source

Beutler Lab

Gene Symbol

Zdhhc4

Ensembl Gene

ENSMUSG00000001844

Gene Name

zinc finger, DHHC domain containing 4

Synonyms

MMRRC Submission

041091-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.120) question?

Stock #

R4305 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

143316489-143329256 bp(-) (GRCm38)

Type of Mutation

intron

DNA Base Change (assembly)

C to T at 143324344 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000124997 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000001900] [ENSMUST00000159813] [ENSMUST00000159941] [ENSMUST00000161915] [ENSMUST00000162066] [ENSMUST00000162941] [ENSMUST00000162358]

AlphaFold

Q9D6H5

Predicted Effect

probably benign
Transcript: ENSMUST00000001900

SMART Domains

Protein: ENSMUSP00000001900
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
low complexity region	97	110	N/A	INTRINSIC
Pfam:zf-DHHC	112	294	5e-38	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159718

Predicted Effect

probably benign
Transcript: ENSMUST00000159813

SMART Domains

Protein: ENSMUSP00000137935
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
low complexity region	97	110	N/A	INTRINSIC
Pfam:zf-DHHC	112	175	3.6e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000159941

SMART Domains

Protein: ENSMUSP00000124026
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
low complexity region	97	110	N/A	INTRINSIC
Pfam:zf-DHHC	112	178	2.2e-16	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160061

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160231

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161199

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161333

Predicted Effect

probably benign
Transcript: ENSMUST00000161915

SMART Domains

Protein: ENSMUSP00000124813
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
transmembrane domain	100	122	N/A	INTRINSIC
Pfam:zf-DHHC	144	294	9.7e-37	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000162066

SMART Domains

Protein: ENSMUSP00000125130
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162284

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162287

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162890

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000198455

Predicted Effect

probably benign
Transcript: ENSMUST00000162941

SMART Domains

Protein: ENSMUSP00000124997
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
low complexity region	97	110	N/A	INTRINSIC
Pfam:zf-DHHC	112	176	4.2e-14	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000162358

SMART Domains

Protein: ENSMUSP00000124416
Gene: ENSMUSG00000001844

Domain	Start	End	E-Value	Type
transmembrane domain	5	24	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
transmembrane domain	100	122	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.2%

Validation Efficiency

92% (36/39)

Allele List at MGI

Other mutations in this stock

Total: 26 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A830018L16Rik	C	A	1: 11,972,076	S440*	probably null	Het
Abl2	T	A	1: 156,641,563	M695K	probably damaging	Het
Asap2	T	C	12: 21,229,481	I426T	probably damaging	Het
Atxn7l1	T	C	12: 33,341,992	M93T	probably damaging	Het
Ccr5	T	C	9: 124,125,074	L238P	possibly damaging	Het
Cd27	A	G	6: 125,234,670	V98A	probably benign	Het
Chrdl2	A	G	7: 100,022,022	T116A	probably damaging	Het
Epha6	T	C	16: 60,526,520		probably null	Het
Fam71f1	A	G	6: 29,326,612	S243G	probably damaging	Het
Gart	T	C	16: 91,633,992	E394G	possibly damaging	Het
Gm5155	T	A	7: 17,905,193	Y372N	probably benign	Het
Gm7173	C	G	X: 79,498,029	K469N	probably damaging	Het
Krt1	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	15: 101,850,378		probably benign	Het
Lpar6	G	A	14: 73,238,941	R114Q	probably damaging	Het
Med23	G	T	10: 24,904,270	E573*	probably null	Het
Mtch2	A	G	2: 90,859,483	I183V	probably benign	Het
Nlrp3	C	T	11: 59,548,010	R138*	probably null	Het
Notch1	T	C	2: 26,477,924	D657G	probably damaging	Het
Olfr1281	A	G	2: 111,329,298	D293G	probably null	Het
Rbm20	A	G	19: 53,843,260	S642G	probably damaging	Het
Tex11	C	A	X: 100,933,415	A487S	possibly damaging	Het
Ttn	T	C	2: 76,918,337	S4123G	probably benign	Het
Ugt3a1	A	C	15: 9,306,274	S170R	possibly damaging	Het
Vmn2r71	G	T	7: 85,624,152	D725Y	probably damaging	Het
Vps9d1	G	T	8: 123,248,237		probably benign	Het
Yeats2	A	G	16: 20,208,422	T808A	probably damaging	Het

Other mutations in Zdhhc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02686:Zdhhc4	APN	5	143320391	missense	probably damaging	1.00
R2022:Zdhhc4	UTSW	5	143321783	missense	probably damaging	1.00
R2138:Zdhhc4	UTSW	5	143324262	nonsense	probably null
R2228:Zdhhc4	UTSW	5	143320407	missense	probably damaging	0.98
R4298:Zdhhc4	UTSW	5	143324242	missense	probably damaging	0.96
R4722:Zdhhc4	UTSW	5	143321781	missense	probably damaging	1.00
R4773:Zdhhc4	UTSW	5	143326176	missense	possibly damaging	0.50
R5000:Zdhhc4	UTSW	5	143324933	missense	probably damaging	0.98
R5063:Zdhhc4	UTSW	5	143316622	missense	probably damaging	1.00
R5341:Zdhhc4	UTSW	5	143326160	missense	probably benign	0.01
R5945:Zdhhc4	UTSW	5	143324886	missense	probably damaging	1.00
R5956:Zdhhc4	UTSW	5	143324849	intron	probably benign
R7284:Zdhhc4	UTSW	5	143321891	missense	probably benign	0.01
R7843:Zdhhc4	UTSW	5	143320276	missense	probably damaging	1.00
R7955:Zdhhc4	UTSW	5	143321864	missense	probably damaging	1.00
R8261:Zdhhc4	UTSW	5	143321833	missense	probably benign	0.17

Predicted Primers

PCR Primer
(F):5'- TTAGCAGCACATAGGGCAG -3'
(R):5'- CCTACATGGCCAGTTTGTTGTC -3'

Sequencing Primer
(F):5'- CAGCACATAGGGCAGGAGAAG -3'
(R):5'- TCTGAGACATGCTTCTGAGACAG -3'

Posted On

2015-06-24