|Institutional Source||Beutler Lab|
|Gene Name||complement component 3a receptor 1|
|Synonyms||C3aR, anaphylatoxin C3a receptor|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R4345 (G1)|
|Chromosomal Location||122847138-122856161 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 122850700 bp|
|Amino Acid Change||Aspartic acid to Glycine at position 186 (D186G)|
|Ref Sequence||ENSEMBL: ENSMUSP00000048092 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000042081]|
|Predicted Effect||probably damaging
AA Change: D186G
PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
AA Change: D186G
|Meta Mutation Damage Score||0.0925|
|Coding Region Coverage||
|Validation Efficiency||96% (47/49)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] C3a is an anaphylatoxin released during activation of the complement system. The protein encoded by this gene is an orphan G protein-coupled receptor for C3a. Binding of C3a by the encoded receptor activates chemotaxis, granule enzyme release, superoxide anion production, and bacterial opsonization. [provided by RefSeq, May 2016]
PHENOTYPE: Homozygous targeted mutants display protective effects against the changes in lung physiology after allergen challenge, increased lethality to endotoxin shock, and elevated IL1B following LPS challenge, supporting the role of C3arin proinflammatory responses. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in C3ar1||
(F):5'- TCCACCATAATGGGGTTGTTG -3'
(R):5'- ATAAGCCAATCTGGTGCCAG -3'
(F):5'- GCTGAATCTAGAGAGAATGAGTCTTC -3'
(R):5'- CTGGTGCCAGAATCATCGAAACG -3'