Mutagenetix > Incidental Mutation

Incidental Mutation 'R4326:Zfp934'

324380

Institutional Source

Beutler Lab

Gene Symbol

Zfp934

Ensembl Gene

ENSMUSG00000074865

Gene Name

zinc finger protein 934

Synonyms

6720457D02Rik

MMRRC Submission

041096-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.126) question?

Stock #

R4326 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

62664609-62727170 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 62665373 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Histidine at position 312 (N312H)

Ref Sequence

ENSEMBL: ENSMUSP00000080837 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076195] [ENSMUST00000082203] [ENSMUST00000101547] [ENSMUST00000220648] [ENSMUST00000221250] [ENSMUST00000222429] [ENSMUST00000222852] [ENSMUST00000222746]

AlphaFold

K7N6H3

Predicted Effect

probably benign
Transcript: ENSMUST00000076195

SMART Domains

Protein: ENSMUSP00000075552
Gene: ENSMUSG00000055228

Domain	Start	End	E-Value	Type
KRAB	4	66	8.26e-16	SMART
ZnF_C2H2	133	155	2.09e-3	SMART
ZnF_C2H2	161	183	1.4e-4	SMART
ZnF_C2H2	189	211	2.57e-3	SMART
ZnF_C2H2	217	239	5.5e-3	SMART
ZnF_C2H2	245	267	7.26e-3	SMART
ZnF_C2H2	273	295	1.4e-4	SMART
ZnF_C2H2	301	323	8.6e-5	SMART
ZnF_C2H2	329	351	1.2e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000082203
AA Change: N312H

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000080837
Gene: ENSMUSG00000074865
AA Change: N312H

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	3e-16	BLAST
ZnF_C2H2	101	123	1.04e-3	SMART
ZnF_C2H2	129	149	1.68e1	SMART
ZnF_C2H2	157	179	2.75e-3	SMART
ZnF_C2H2	185	207	1.98e-4	SMART
ZnF_C2H2	213	235	3.21e-4	SMART
ZnF_C2H2	241	263	3.44e-4	SMART
ZnF_C2H2	269	292	4.98e-1	SMART
ZnF_C2H2	298	320	4.87e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000101547
AA Change: N455H

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000099083
Gene: ENSMUSG00000074865
AA Change: N455H

Domain	Start	End	E-Value	Type
KRAB	3	65	5.01e-15	SMART
ZnF_C2H2	132	154	1.04e-3	SMART
ZnF_C2H2	160	180	1.68e1	SMART
ZnF_C2H2	188	210	2.75e-3	SMART
ZnF_C2H2	216	238	1.98e-4	SMART
ZnF_C2H2	244	266	3.21e-4	SMART
ZnF_C2H2	272	294	3.44e-4	SMART
ZnF_C2H2	300	322	2.27e-4	SMART
ZnF_C2H2	328	350	3.44e-4	SMART
ZnF_C2H2	356	378	9.22e-5	SMART
ZnF_C2H2	384	406	2.43e-4	SMART
ZnF_C2H2	412	434	1.3e-4	SMART
ZnF_C2H2	440	462	4.87e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000220648
AA Change: N423H

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000221250

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221494

Predicted Effect

probably benign
Transcript: ENSMUST00000222429

Predicted Effect

probably benign
Transcript: ENSMUST00000222852

Predicted Effect

probably benign
Transcript: ENSMUST00000222746

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.4%

Validation Efficiency

98% (57/58)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcd3	A	G	3: 121,555,119 (GRCm39)	V616A	probably benign	Het
Abhd17a	A	G	10: 80,419,884 (GRCm39)	S241P	probably benign	Het
Adamts15	T	C	9: 30,815,814 (GRCm39)	S681G	probably benign	Het
Arap2	A	T	5: 62,779,206 (GRCm39)	H1461Q	possibly damaging	Het
Armc3	A	G	2: 19,305,284 (GRCm39)	K681E	probably damaging	Het
Arrdc5	T	C	17: 56,601,420 (GRCm39)	E235G	possibly damaging	Het
Atxn1	T	C	13: 46,119,443 (GRCm39)		probably benign	Het
C9orf72	T	A	4: 35,225,985 (GRCm39)		probably benign	Het
Capn13	C	A	17: 73,638,103 (GRCm39)	K433N	probably benign	Het
Ccdc113	G	A	8: 96,283,896 (GRCm39)	M323I	probably benign	Het
Cd55	A	T	1: 130,380,220 (GRCm39)	C253S	probably damaging	Het
Cyp2j8	T	A	4: 96,395,566 (GRCm39)	T20S	probably benign	Het
Dnah8	G	A	17: 30,971,066 (GRCm39)	V2707M	probably benign	Het
Erlec1	C	T	11: 30,899,972 (GRCm39)	E166K	probably benign	Het
Fastkd2	A	G	1: 63,791,516 (GRCm39)	K675E	probably benign	Het
Gid4	G	A	11: 60,308,662 (GRCm39)	V61M	possibly damaging	Het
Glt28d2	G	A	3: 85,779,393 (GRCm39)	Q27*	probably null	Het
Ipo8	A	T	6: 148,701,662 (GRCm39)		probably benign	Het
Isx	A	G	8: 75,600,284 (GRCm39)	I6V	probably benign	Het
Itsn1	A	G	16: 91,650,743 (GRCm39)		probably benign	Het
Klhl40	A	G	9: 121,607,956 (GRCm39)	D372G	probably benign	Het
Krt80	C	T	15: 101,250,189 (GRCm39)	V19M	possibly damaging	Het
Lingo2	T	A	4: 35,708,462 (GRCm39)	D506V	probably damaging	Het
Lmo7	A	T	14: 102,137,510 (GRCm39)	E504D	possibly damaging	Het
Lpcat4	G	A	2: 112,076,737 (GRCm39)	E454K	probably benign	Het
Macf1	C	T	4: 123,276,005 (GRCm39)	A4176T	probably damaging	Het
Noxo1	G	A	17: 24,917,937 (GRCm39)	R81H	probably benign	Het
Or10ab4	T	C	7: 107,654,362 (GRCm39)	Y58H	probably damaging	Het
Or7g34	T	A	9: 19,478,318 (GRCm39)	M121L	possibly damaging	Het
Pafah1b1	T	C	11: 74,573,066 (GRCm39)	T333A	probably benign	Het
Pak1ip1	A	G	13: 41,158,232 (GRCm39)	D35G	possibly damaging	Het
Pak3	T	C	X: 142,516,205 (GRCm39)		probably null	Het
Pcdhb9	A	T	18: 37,534,875 (GRCm39)	S290C	probably benign	Het
Pcdhb9	G	T	18: 37,534,876 (GRCm39)	S290I	probably benign	Het
Ppp2r2d	T	A	7: 138,470,214 (GRCm39)	V25D	probably damaging	Het
Prdm15	G	T	16: 97,607,715 (GRCm39)	N709K	probably damaging	Het
Ptprs	C	A	17: 56,754,468 (GRCm39)	A191S	possibly damaging	Het
Rrs1	G	T	1: 9,616,566 (GRCm39)	R273L	possibly damaging	Het
Sin3a	T	C	9: 57,002,642 (GRCm39)	L178P	probably damaging	Het
Slc13a1	A	G	6: 24,103,478 (GRCm39)	V355A	probably benign	Het
Spen	T	G	4: 141,204,683 (GRCm39)	N1315H	unknown	Het
Ssrp1	A	G	2: 84,870,561 (GRCm39)		probably benign	Het
St7	G	A	6: 17,819,287 (GRCm39)	V64M	probably damaging	Het
Syne2	C	A	12: 75,999,516 (GRCm39)	A2304E	probably damaging	Het
Tcf25	T	A	8: 124,127,882 (GRCm39)	L622*	probably null	Het
Tmem107	G	T	11: 68,962,301 (GRCm39)		probably null	Het
Tmem62	T	A	2: 120,810,991 (GRCm39)	N156K	probably damaging	Het
Wdr90	G	T	17: 26,072,705 (GRCm39)	R884S	probably benign	Het
Zfp184	T	C	13: 22,144,072 (GRCm39)	Y593H	probably damaging	Het
Zfta	A	G	19: 7,398,591 (GRCm39)		probably benign	Het

Other mutations in Zfp934

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1915:Zfp934	UTSW	13	62,665,769 (GRCm39)	missense	probably damaging	1.00
R1957:Zfp934	UTSW	13	62,666,108 (GRCm39)	missense	possibly damaging	0.83
R2113:Zfp934	UTSW	13	62,666,507 (GRCm39)	missense	probably damaging	0.98
R2145:Zfp934	UTSW	13	62,665,648 (GRCm39)	missense	probably damaging	1.00
R3732:Zfp934	UTSW	13	62,665,599 (GRCm39)	missense	probably damaging	1.00
R3797:Zfp934	UTSW	13	62,665,702 (GRCm39)	missense	probably benign
R4327:Zfp934	UTSW	13	62,665,373 (GRCm39)	missense	probably benign	0.01
R4909:Zfp934	UTSW	13	62,665,768 (GRCm39)	missense	probably damaging	1.00
R5236:Zfp934	UTSW	13	62,665,527 (GRCm39)	missense	probably damaging	1.00
R5237:Zfp934	UTSW	13	62,665,652 (GRCm39)	missense	probably damaging	1.00
R6305:Zfp934	UTSW	13	62,666,370 (GRCm39)	missense	probably damaging	0.99
R6729:Zfp934	UTSW	13	62,640,746 (GRCm39)	missense	probably damaging	1.00
R7072:Zfp934	UTSW	13	62,668,339 (GRCm39)	missense	probably damaging	0.99
R7186:Zfp934	UTSW	13	62,640,204 (GRCm39)	missense	probably benign	0.12
R7235:Zfp934	UTSW	13	62,665,964 (GRCm39)	missense
R7453:Zfp934	UTSW	13	62,666,517 (GRCm39)	missense	probably benign	0.40
R7552:Zfp934	UTSW	13	62,640,705 (GRCm39)	missense	probably damaging	1.00
R7589:Zfp934	UTSW	13	62,666,130 (GRCm39)	missense
R7624:Zfp934	UTSW	13	62,666,338 (GRCm39)	missense	probably benign	0.27
R7651:Zfp934	UTSW	13	62,666,327 (GRCm39)	missense	probably benign
R7780:Zfp934	UTSW	13	62,666,358 (GRCm39)	missense	possibly damaging	0.92
R7857:Zfp934	UTSW	13	62,665,361 (GRCm39)	missense
R7891:Zfp934	UTSW	13	62,668,003 (GRCm39)	missense	probably benign	0.35
R8130:Zfp934	UTSW	13	62,667,985 (GRCm39)	critical splice donor site	probably null
R8298:Zfp934	UTSW	13	62,666,295 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- CACAATCCAATTCCTTCGTAGC -3'
(R):5'- CAGTGTGATAAAGCCTTTTCTGAG -3'

Sequencing Primer
(F):5'- ACACACACTGAATAAACTTGTAAGG -3'
(R):5'- AGCCTTTTCTGAGAAATGTAGTCTCC -3'

Posted On

2015-06-24