Incidental Mutation 'R4329:Zfp827'
| ID |
324523 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp827
|
| Ensembl Gene |
ENSMUSG00000071064 |
| Gene Name |
zinc finger protein 827 |
| Synonyms |
D630040G17Rik, 2810449M09Rik |
| MMRRC Submission |
041663-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably essential
(E-score: 0.920)
|
| Stock # |
R4329 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
8 |
| Chromosomal Location |
79755066-79920395 bp(+) (GRCm39) |
| Type of Mutation |
utr 3 prime |
| DNA Base Change (assembly) |
G to A
at 79916463 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000120913
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000098614]
[ENSMUST00000119254]
[ENSMUST00000148713]
|
| AlphaFold |
Q505G8 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000098614
|
| SMART Domains |
Protein: ENSMUSP00000096214
Gene: ENSMUSG00000071064
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
102 |
128 |
N/A |
INTRINSIC |
|
low complexity region
|
226 |
256 |
N/A |
INTRINSIC |
|
low complexity region
|
271 |
298 |
N/A |
INTRINSIC |
|
low complexity region
|
306 |
342 |
N/A |
INTRINSIC |
|
low complexity region
|
343 |
348 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
371 |
393 |
6.78e-3 |
SMART |
|
ZnF_C2H2
|
399 |
421 |
2.99e-4 |
SMART |
|
ZnF_C2H2
|
430 |
452 |
5.42e-2 |
SMART |
|
internal_repeat_2
|
561 |
585 |
3.31e-7 |
PROSPERO |
|
low complexity region
|
613 |
627 |
N/A |
INTRINSIC |
|
internal_repeat_2
|
719 |
743 |
3.31e-7 |
PROSPERO |
|
ZnF_C2H2
|
814 |
836 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
842 |
864 |
4.72e-2 |
SMART |
|
ZnF_C2H2
|
894 |
916 |
1.64e-1 |
SMART |
|
ZnF_C2H2
|
926 |
949 |
7.89e0 |
SMART |
|
low complexity region
|
956 |
974 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
1016 |
1038 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
1044 |
1066 |
3.07e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000119254
|
| SMART Domains |
Protein: ENSMUSP00000113199
Gene: ENSMUSG00000071064
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
102 |
128 |
N/A |
INTRINSIC |
|
low complexity region
|
226 |
256 |
N/A |
INTRINSIC |
|
low complexity region
|
271 |
298 |
N/A |
INTRINSIC |
|
low complexity region
|
306 |
342 |
N/A |
INTRINSIC |
|
low complexity region
|
343 |
348 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
371 |
393 |
6.78e-3 |
SMART |
|
ZnF_C2H2
|
399 |
421 |
2.99e-4 |
SMART |
|
ZnF_C2H2
|
430 |
452 |
5.42e-2 |
SMART |
|
internal_repeat_2
|
561 |
585 |
3.25e-7 |
PROSPERO |
|
low complexity region
|
613 |
627 |
N/A |
INTRINSIC |
|
internal_repeat_2
|
719 |
743 |
3.25e-7 |
PROSPERO |
|
ZnF_C2H2
|
814 |
836 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
842 |
864 |
4.72e-2 |
SMART |
|
ZnF_C2H2
|
894 |
916 |
1.64e-1 |
SMART |
|
ZnF_C2H2
|
926 |
949 |
7.89e0 |
SMART |
|
low complexity region
|
956 |
974 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
1016 |
1038 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
1044 |
1066 |
3.07e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000129613
|
| SMART Domains |
Protein: ENSMUSP00000119511
Gene: ENSMUSG00000071064
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
35 |
57 |
1.64e-1 |
SMART |
|
ZnF_C2H2
|
67 |
90 |
7.89e0 |
SMART |
|
low complexity region
|
97 |
115 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
157 |
179 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
185 |
207 |
3.07e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000145827
|
| SMART Domains |
Protein: ENSMUSP00000114208
Gene: ENSMUSG00000071064
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
77 |
99 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
111 |
133 |
4.72e-2 |
SMART |
|
ZnF_C2H2
|
163 |
185 |
1.64e-1 |
SMART |
|
ZnF_C2H2
|
195 |
218 |
7.89e0 |
SMART |
|
low complexity region
|
225 |
243 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
285 |
307 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
313 |
335 |
3.07e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000148713
|
| SMART Domains |
Protein: ENSMUSP00000120913
Gene: ENSMUSG00000071064
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
106 |
128 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
134 |
156 |
4.72e-2 |
SMART |
|
ZnF_C2H2
|
186 |
208 |
1.64e-1 |
SMART |
|
ZnF_C2H2
|
218 |
241 |
7.89e0 |
SMART |
|
low complexity region
|
248 |
266 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
308 |
330 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
336 |
358 |
3.07e-1 |
SMART |
|
| Predicted Effect |
|
| SMART Domains |
Protein: ENSMUSP00000120562
Gene: ENSMUSG00000071064
AA Change: C346Y
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
31 |
53 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
59 |
81 |
4.72e-2 |
SMART |
|
ZnF_C2H2
|
111 |
133 |
1.64e-1 |
SMART |
|
ZnF_C2H2
|
143 |
166 |
7.89e0 |
SMART |
|
low complexity region
|
173 |
191 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
233 |
255 |
1.26e-2 |
SMART |
|
ZnF_C2H2
|
261 |
283 |
3.07e-1 |
SMART |
|
ZnF_C2H2
|
290 |
312 |
9.44e-2 |
SMART |
|
ZnF_C2H2
|
317 |
339 |
4.34e-1 |
SMART |
|
ZnF_C2H2
|
345 |
367 |
7.9e-4 |
SMART |
|
ZnF_C2H2
|
373 |
396 |
2.53e-2 |
SMART |
|
ZnF_C2H2
|
402 |
424 |
2.45e0 |
SMART |
|
low complexity region
|
444 |
466 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.2752 |
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.5%
- 20x: 95.9%
|
| Validation Efficiency |
96% (68/71) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 56 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 2900026A02Rik |
C |
A |
5: 113,243,455 (GRCm39) |
|
probably null |
Het |
| Adamts15 |
T |
C |
9: 30,815,814 (GRCm39) |
S681G |
probably benign |
Het |
| Ampd2 |
C |
A |
3: 107,985,103 (GRCm39) |
|
probably benign |
Het |
| Ang2 |
A |
T |
14: 51,433,325 (GRCm39) |
I19N |
possibly damaging |
Het |
| Brd10 |
G |
T |
19: 29,720,961 (GRCm39) |
T688K |
probably benign |
Het |
| Cacna1s |
A |
C |
1: 136,046,771 (GRCm39) |
D1819A |
probably benign |
Het |
| Cand2 |
C |
A |
6: 115,776,949 (GRCm39) |
H1112Q |
possibly damaging |
Het |
| Catsperd |
T |
A |
17: 56,961,517 (GRCm39) |
I413K |
possibly damaging |
Het |
| Ccdc142 |
T |
A |
6: 83,083,997 (GRCm39) |
|
probably benign |
Het |
| Cd55 |
A |
T |
1: 130,380,220 (GRCm39) |
C253S |
probably damaging |
Het |
| Celsr3 |
C |
T |
9: 108,723,248 (GRCm39) |
R2847C |
probably benign |
Het |
| Cep290 |
C |
T |
10: 100,373,530 (GRCm39) |
S1270L |
probably damaging |
Het |
| Chka |
T |
C |
19: 3,925,803 (GRCm39) |
|
probably benign |
Het |
| Cntnap5b |
T |
A |
1: 99,999,888 (GRCm39) |
D215E |
probably damaging |
Het |
| Col13a1 |
T |
C |
10: 61,699,758 (GRCm39) |
T476A |
unknown |
Het |
| Cspg4 |
T |
C |
9: 56,799,749 (GRCm39) |
F1539L |
probably damaging |
Het |
| Cwf19l2 |
A |
T |
9: 3,458,878 (GRCm39) |
I776F |
probably damaging |
Het |
| Dchs1 |
A |
T |
7: 105,402,966 (GRCm39) |
I3192N |
probably damaging |
Het |
| Dnah7c |
T |
A |
1: 46,688,441 (GRCm39) |
D1870E |
probably benign |
Het |
| Drc1 |
A |
T |
5: 30,513,002 (GRCm39) |
I359L |
probably benign |
Het |
| Esp1 |
A |
G |
17: 41,039,768 (GRCm39) |
M18V |
probably benign |
Het |
| Exoc1 |
T |
A |
5: 76,715,822 (GRCm39) |
F789L |
probably damaging |
Het |
| Fbxw22 |
T |
A |
9: 109,213,111 (GRCm39) |
T279S |
probably damaging |
Het |
| Frem1 |
G |
A |
4: 82,904,774 (GRCm39) |
A880V |
probably benign |
Het |
| Gm29514 |
A |
G |
1: 146,296,429 (GRCm39) |
|
noncoding transcript |
Het |
| Grm7 |
T |
C |
6: 110,891,325 (GRCm39) |
L186P |
probably damaging |
Het |
| Ipo8 |
A |
T |
6: 148,701,662 (GRCm39) |
|
probably benign |
Het |
| Klk4 |
A |
G |
7: 43,533,830 (GRCm39) |
D202G |
probably damaging |
Het |
| Marchf6 |
C |
T |
15: 31,498,887 (GRCm39) |
E137K |
probably benign |
Het |
| Mpp3 |
T |
C |
11: 101,914,337 (GRCm39) |
|
probably benign |
Het |
| Mroh2b |
T |
A |
15: 4,960,861 (GRCm39) |
Y808N |
probably damaging |
Het |
| Myom1 |
C |
T |
17: 71,343,348 (GRCm39) |
L182F |
probably damaging |
Het |
| Nova1 |
A |
T |
12: 46,767,615 (GRCm39) |
I102N |
unknown |
Het |
| Or7g34 |
T |
A |
9: 19,478,318 (GRCm39) |
M121L |
possibly damaging |
Het |
| Pak3 |
T |
C |
X: 142,516,205 (GRCm39) |
|
probably null |
Het |
| Palm |
G |
A |
10: 79,643,520 (GRCm39) |
G83S |
probably benign |
Het |
| Patl2 |
C |
T |
2: 121,958,018 (GRCm39) |
S80N |
probably benign |
Het |
| Pcdhb9 |
A |
T |
18: 37,534,875 (GRCm39) |
S290C |
probably benign |
Het |
| Pcdhb9 |
G |
T |
18: 37,534,876 (GRCm39) |
S290I |
probably benign |
Het |
| Pdgfrb |
A |
T |
18: 61,204,792 (GRCm39) |
I551F |
possibly damaging |
Het |
| Pkmyt1 |
T |
A |
17: 23,951,709 (GRCm39) |
Y88N |
probably damaging |
Het |
| Pnkp |
T |
C |
7: 44,508,018 (GRCm39) |
S114P |
probably benign |
Het |
| Rdm1 |
T |
A |
11: 101,521,734 (GRCm39) |
V92E |
probably damaging |
Het |
| Sema4d |
T |
C |
13: 51,857,340 (GRCm39) |
R631G |
probably benign |
Het |
| Sin3a |
T |
C |
9: 57,002,642 (GRCm39) |
L178P |
probably damaging |
Het |
| Skint9 |
A |
C |
4: 112,249,062 (GRCm39) |
L122R |
probably damaging |
Het |
| Slc16a13 |
T |
C |
11: 70,108,723 (GRCm39) |
N369S |
probably benign |
Het |
| Strada |
A |
G |
11: 106,077,999 (GRCm39) |
|
probably benign |
Het |
| Tnxb |
C |
A |
17: 34,912,838 (GRCm39) |
S1784Y |
probably damaging |
Het |
| Trpc2 |
T |
C |
7: 101,736,727 (GRCm39) |
V318A |
probably damaging |
Het |
| Ttc3 |
G |
A |
16: 94,267,820 (GRCm39) |
R1589H |
probably damaging |
Het |
| Ttyh1 |
A |
T |
7: 4,133,580 (GRCm39) |
D295V |
probably damaging |
Het |
| Unc5b |
A |
G |
10: 60,618,969 (GRCm39) |
Y58H |
probably damaging |
Het |
| Xylt1 |
G |
A |
7: 117,255,684 (GRCm39) |
G752D |
probably damaging |
Het |
| Yipf7 |
A |
T |
5: 69,678,465 (GRCm39) |
L55Q |
probably damaging |
Het |
| Zfta |
A |
G |
19: 7,398,591 (GRCm39) |
|
probably benign |
Het |
|
| Other mutations in Zfp827 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01448:Zfp827
|
APN |
8 |
79,787,362 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
IGL01545:Zfp827
|
APN |
8 |
79,797,063 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01552:Zfp827
|
APN |
8 |
79,802,820 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02261:Zfp827
|
APN |
8 |
79,906,708 (GRCm39) |
missense |
probably damaging |
0.97 |
|
IGL02451:Zfp827
|
APN |
8 |
79,787,601 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03130:Zfp827
|
APN |
8 |
79,787,586 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03411:Zfp827
|
APN |
8 |
79,803,116 (GRCm39) |
missense |
probably damaging |
0.99 |
|
E0354:Zfp827
|
UTSW |
8 |
79,863,206 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0502:Zfp827
|
UTSW |
8 |
79,905,706 (GRCm39) |
splice site |
probably null |
|
|
R0547:Zfp827
|
UTSW |
8 |
79,786,939 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0926:Zfp827
|
UTSW |
8 |
79,844,821 (GRCm39) |
missense |
probably benign |
0.00 |
|
R0975:Zfp827
|
UTSW |
8 |
79,787,814 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1305:Zfp827
|
UTSW |
8 |
79,787,523 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R1462:Zfp827
|
UTSW |
8 |
79,803,108 (GRCm39) |
missense |
probably benign |
|
|
R1462:Zfp827
|
UTSW |
8 |
79,803,108 (GRCm39) |
missense |
probably benign |
|
|
R1638:Zfp827
|
UTSW |
8 |
79,802,975 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R1714:Zfp827
|
UTSW |
8 |
79,787,202 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2044:Zfp827
|
UTSW |
8 |
79,802,865 (GRCm39) |
missense |
probably benign |
|
|
R2132:Zfp827
|
UTSW |
8 |
79,912,350 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R3844:Zfp827
|
UTSW |
8 |
79,863,248 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4629:Zfp827
|
UTSW |
8 |
79,787,011 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4873:Zfp827
|
UTSW |
8 |
79,787,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4875:Zfp827
|
UTSW |
8 |
79,787,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4936:Zfp827
|
UTSW |
8 |
79,787,812 (GRCm39) |
missense |
probably benign |
|
|
R4965:Zfp827
|
UTSW |
8 |
79,787,910 (GRCm39) |
missense |
probably benign |
|
|
R5103:Zfp827
|
UTSW |
8 |
79,797,032 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5366:Zfp827
|
UTSW |
8 |
79,912,333 (GRCm39) |
missense |
possibly damaging |
0.94 |
|
R5794:Zfp827
|
UTSW |
8 |
79,797,071 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5825:Zfp827
|
UTSW |
8 |
79,905,645 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6118:Zfp827
|
UTSW |
8 |
79,803,067 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R6236:Zfp827
|
UTSW |
8 |
79,797,105 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6263:Zfp827
|
UTSW |
8 |
79,905,702 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6306:Zfp827
|
UTSW |
8 |
79,787,324 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6490:Zfp827
|
UTSW |
8 |
79,916,606 (GRCm39) |
utr 3 prime |
probably benign |
|
|
R6497:Zfp827
|
UTSW |
8 |
79,906,757 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7250:Zfp827
|
UTSW |
8 |
79,916,721 (GRCm39) |
missense |
|
|
|
R7290:Zfp827
|
UTSW |
8 |
79,916,442 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R7443:Zfp827
|
UTSW |
8 |
79,917,047 (GRCm39) |
missense |
|
|
|
R7708:Zfp827
|
UTSW |
8 |
79,902,591 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7754:Zfp827
|
UTSW |
8 |
79,916,958 (GRCm39) |
missense |
|
|
|
R7836:Zfp827
|
UTSW |
8 |
79,912,979 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7995:Zfp827
|
UTSW |
8 |
79,844,887 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R8162:Zfp827
|
UTSW |
8 |
79,787,206 (GRCm39) |
nonsense |
probably null |
|
|
R8747:Zfp827
|
UTSW |
8 |
79,755,316 (GRCm39) |
start codon destroyed |
probably null |
|
|
R8798:Zfp827
|
UTSW |
8 |
79,916,463 (GRCm39) |
utr 3 prime |
probably benign |
|
|
R8980:Zfp827
|
UTSW |
8 |
79,803,092 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9099:Zfp827
|
UTSW |
8 |
79,917,107 (GRCm39) |
missense |
|
|
|
R9178:Zfp827
|
UTSW |
8 |
79,818,564 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9246:Zfp827
|
UTSW |
8 |
79,803,132 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R9277:Zfp827
|
UTSW |
8 |
79,787,029 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R9318:Zfp827
|
UTSW |
8 |
79,844,982 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R9339:Zfp827
|
UTSW |
8 |
79,844,887 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9425:Zfp827
|
UTSW |
8 |
79,905,588 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9499:Zfp827
|
UTSW |
8 |
79,787,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9544:Zfp827
|
UTSW |
8 |
79,905,604 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9551:Zfp827
|
UTSW |
8 |
79,787,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9552:Zfp827
|
UTSW |
8 |
79,787,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9588:Zfp827
|
UTSW |
8 |
79,905,604 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9665:Zfp827
|
UTSW |
8 |
79,906,756 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
| Predicted Primers |
PCR Primer
(F):5'- GGACCTTCCATACTAAAGCTCC -3'
(R):5'- TCTTGAACAGGCACTCTCCG -3'
Sequencing Primer
(F):5'- TACTAAAGCTCCCTCCAATTGC -3'
(R):5'- TCTCCGCAGCTGAAGGTCTG -3'
|
| Posted On |
2015-06-24 |
Incidental Mutation