Mutagenetix > Incidental Mutation

Incidental Mutation 'R4357:Vmn2r59'

324606

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r59

Ensembl Gene

ENSMUSG00000092032

Gene Name

vomeronasal 2, receptor 59

Synonyms

EG628444

MMRRC Submission

041109-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R4357 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

41661216-41708405 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 41661644 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 724 (P724S)

Ref Sequence

ENSEMBL: ENSMUSP00000131856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168489]

AlphaFold

E9PUT5

Predicted Effect

probably damaging
Transcript: ENSMUST00000168489
AA Change: P724S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000131856
Gene: ENSMUSG00000092032
AA Change: P724S

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.8e-44	PFAM
Pfam:NCD3G	514	567	4.3e-23	PFAM
Pfam:7tm_3	600	835	5.4e-53	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam20	A	G	8: 41,248,084 (GRCm39)	T65A	possibly damaging	Het
Alpk1	C	T	3: 127,523,022 (GRCm39)	V7M	probably damaging	Het
Ap4m1	A	G	5: 138,171,311 (GRCm39)	E125G	probably damaging	Het
Atp13a2	C	A	4: 140,729,215 (GRCm39)	D599E	probably benign	Het
Brme1	T	C	8: 84,886,221 (GRCm39)	L59P	probably benign	Het
Camkv	G	A	9: 107,825,145 (GRCm39)	G354E	probably benign	Het
Casp8ap2	T	A	4: 32,646,150 (GRCm39)	M1741K	probably benign	Het
Chtf18	T	C	17: 25,938,106 (GRCm39)	D119G	probably benign	Het
Cnga1	T	A	5: 72,775,595 (GRCm39)	D42V	probably damaging	Het
Cysltr2	T	C	14: 73,267,084 (GRCm39)	I209V	probably benign	Het
Defa27	A	T	8: 21,805,608 (GRCm39)	Q16L	probably null	Het
Dnaja3	T	C	16: 4,517,731 (GRCm39)	I321T	probably damaging	Het
Eef2	GCCC	GCCCC	10: 81,014,601 (GRCm39)		probably null	Het
Eif2ak3	T	A	6: 70,861,859 (GRCm39)	I467N	probably damaging	Het
Eif5b	T	A	1: 38,089,339 (GRCm39)	V1105E	probably damaging	Het
Gfod2	T	C	8: 106,444,177 (GRCm39)	N122S	possibly damaging	Het
Gm10803	A	G	2: 93,394,350 (GRCm39)	R41G	unknown	Het
Gm38392	A	T	3: 88,154,741 (GRCm39)	I221N	probably damaging	Het
Grm7	G	T	6: 110,623,309 (GRCm39)	V161F	probably damaging	Het
Hsd17b6	G	T	10: 127,829,637 (GRCm39)		probably null	Het
Ids	C	A	X: 69,389,950 (GRCm39)	G506C	probably damaging	Het
Ifna15	G	T	4: 88,476,079 (GRCm39)	T135N	probably benign	Het
Igsf9b	G	A	9: 27,220,774 (GRCm39)	V47I	possibly damaging	Het
Kif24	A	G	4: 41,413,827 (GRCm39)		probably null	Het
Lats2	A	G	14: 57,936,840 (GRCm39)	S550P	probably damaging	Het
Lmo7	A	G	14: 102,125,091 (GRCm39)	R406G	probably null	Het
Lpcat2	C	A	8: 93,599,734 (GRCm39)	P234Q	probably benign	Het
Lrp10	C	T	14: 54,705,823 (GRCm39)	R338C	probably damaging	Het
Lrrfip2	A	G	9: 111,028,755 (GRCm39)	E326G	probably damaging	Het
Megf8	T	A	7: 25,055,174 (GRCm39)	I1969N	probably benign	Het
Mib1	A	G	18: 10,751,844 (GRCm39)	N242S	probably benign	Het
Ngf	A	G	3: 102,427,521 (GRCm39)	E94G	probably benign	Het
Oas2	C	T	5: 120,876,734 (GRCm39)		probably null	Het
Odf2	A	G	2: 29,782,256 (GRCm39)	T75A	probably benign	Het
Or4f60	A	G	2: 111,902,583 (GRCm39)	L115P	probably damaging	Het
Or5w11	T	A	2: 87,458,810 (GRCm39)	M1K	probably null	Het
Pi4ka	T	A	16: 17,185,303 (GRCm39)	I266F	probably benign	Het
Prps2	T	A	X: 166,146,545 (GRCm39)	K176*	probably null	Het
Psmd2	T	G	16: 20,475,402 (GRCm39)	D393E	probably benign	Het
Rabep2	A	G	7: 126,047,397 (GRCm39)	I753T	probably damaging	Het
Rara	A	C	11: 98,858,937 (GRCm39)	I129L	probably damaging	Het
Rasgrf2	C	A	13: 92,038,796 (GRCm39)	D1017Y	probably damaging	Het
Slc15a4	A	G	5: 127,681,600 (GRCm39)		probably null	Het
Spmip4	T	C	6: 50,551,190 (GRCm39)	T420A	probably benign	Het
Spsb1	T	C	4: 149,991,232 (GRCm39)	H112R	probably damaging	Het
Sptlc1	A	T	13: 53,528,068 (GRCm39)	I32K	probably damaging	Het
Srd5a3	T	A	5: 76,295,547 (GRCm39)	F79Y	probably damaging	Het
Ssrp1	A	T	2: 84,871,495 (GRCm39)	M306L	probably benign	Het
Sulf2	T	C	2: 165,919,497 (GRCm39)	E817G	probably benign	Het
Tiam2	C	A	17: 3,501,128 (GRCm39)	D922E	probably damaging	Het
Tle4	A	G	19: 14,445,625 (GRCm39)	V207A	probably benign	Het
Ttll12	A	T	15: 83,465,958 (GRCm39)	C413S	probably damaging	Het
Usp45	T	A	4: 21,834,350 (GRCm39)	Y809*	probably null	Het
Vrk3	C	T	7: 44,424,866 (GRCm39)	T427M	probably benign	Het

Other mutations in Vmn2r59

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Vmn2r59	APN	7	41,661,488 (GRCm39)	missense	possibly damaging	0.91
IGL01432:Vmn2r59	APN	7	41,661,983 (GRCm39)	missense	possibly damaging	0.82
IGL02119:Vmn2r59	APN	7	41,695,593 (GRCm39)	missense	probably benign	0.36
IGL02216:Vmn2r59	APN	7	41,661,817 (GRCm39)	missense	probably damaging	1.00
IGL02327:Vmn2r59	APN	7	41,661,655 (GRCm39)	missense	probably benign
IGL03346:Vmn2r59	APN	7	41,693,253 (GRCm39)	missense	probably benign	0.00
IGL03411:Vmn2r59	APN	7	41,708,340 (GRCm39)	missense	probably benign	0.43
IGL03412:Vmn2r59	APN	7	41,661,862 (GRCm39)	missense	probably benign
PIT4366001:Vmn2r59	UTSW	7	41,695,205 (GRCm39)	missense	possibly damaging	0.91
R0068:Vmn2r59	UTSW	7	41,695,725 (GRCm39)	missense	probably damaging	0.99
R0094:Vmn2r59	UTSW	7	41,661,722 (GRCm39)	missense	probably benign	0.07
R0179:Vmn2r59	UTSW	7	41,696,432 (GRCm39)	nonsense	probably null
R0370:Vmn2r59	UTSW	7	41,662,150 (GRCm39)	missense	probably benign	0.23
R0412:Vmn2r59	UTSW	7	41,695,916 (GRCm39)	splice site	probably benign
R0465:Vmn2r59	UTSW	7	41,696,332 (GRCm39)	missense	probably benign
R0487:Vmn2r59	UTSW	7	41,696,528 (GRCm39)	nonsense	probably null
R0576:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R0632:Vmn2r59	UTSW	7	41,708,308 (GRCm39)	missense	probably damaging	1.00
R1356:Vmn2r59	UTSW	7	41,661,218 (GRCm39)	makesense	probably null
R1387:Vmn2r59	UTSW	7	41,695,521 (GRCm39)	missense	probably damaging	1.00
R1388:Vmn2r59	UTSW	7	41,695,133 (GRCm39)	missense	probably benign	0.01
R1435:Vmn2r59	UTSW	7	41,695,629 (GRCm39)	missense	possibly damaging	0.50
R1750:Vmn2r59	UTSW	7	41,695,251 (GRCm39)	missense	possibly damaging	0.50
R2020:Vmn2r59	UTSW	7	41,693,203 (GRCm39)	missense	probably damaging	1.00
R2249:Vmn2r59	UTSW	7	41,708,326 (GRCm39)	missense	probably benign	0.00
R2256:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2257:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2441:Vmn2r59	UTSW	7	41,695,570 (GRCm39)	missense	probably benign	0.00
R2511:Vmn2r59	UTSW	7	41,693,190 (GRCm39)	missense	probably damaging	1.00
R2860:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R2861:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R3690:Vmn2r59	UTSW	7	41,661,370 (GRCm39)	missense	possibly damaging	0.77
R3912:Vmn2r59	UTSW	7	41,695,744 (GRCm39)	missense	probably benign	0.00
R4167:Vmn2r59	UTSW	7	41,670,732 (GRCm39)	intron	probably benign
R4445:Vmn2r59	UTSW	7	41,691,874 (GRCm39)	missense	probably damaging	1.00
R4542:Vmn2r59	UTSW	7	41,695,497 (GRCm39)	missense	possibly damaging	0.93
R4587:Vmn2r59	UTSW	7	41,695,648 (GRCm39)	missense	probably benign	0.00
R4616:Vmn2r59	UTSW	7	41,661,862 (GRCm39)	missense	probably benign
R4653:Vmn2r59	UTSW	7	41,693,228 (GRCm39)	missense	probably benign	0.19
R4703:Vmn2r59	UTSW	7	41,661,686 (GRCm39)	missense	probably benign	0.01
R4895:Vmn2r59	UTSW	7	41,695,218 (GRCm39)	missense	probably damaging	0.98
R4910:Vmn2r59	UTSW	7	41,693,077 (GRCm39)	missense	probably benign
R5045:Vmn2r59	UTSW	7	41,695,496 (GRCm39)	missense	possibly damaging	0.93
R5105:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R5153:Vmn2r59	UTSW	7	41,691,834 (GRCm39)	critical splice donor site	probably null
R5566:Vmn2r59	UTSW	7	41,696,247 (GRCm39)	missense	possibly damaging	0.92
R5586:Vmn2r59	UTSW	7	41,695,105 (GRCm39)	missense	probably benign	0.12
R5606:Vmn2r59	UTSW	7	41,695,318 (GRCm39)	missense	probably benign	0.27
R5616:Vmn2r59	UTSW	7	41,708,191 (GRCm39)	splice site	probably null
R5625:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R5696:Vmn2r59	UTSW	7	41,695,468 (GRCm39)	missense	probably benign	0.00
R5982:Vmn2r59	UTSW	7	41,695,491 (GRCm39)	missense	probably benign	0.00
R6106:Vmn2r59	UTSW	7	41,661,749 (GRCm39)	nonsense	probably null
R6196:Vmn2r59	UTSW	7	41,661,679 (GRCm39)	missense	probably benign	0.36
R6228:Vmn2r59	UTSW	7	41,691,835 (GRCm39)	critical splice donor site	probably null
R6590:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6625:Vmn2r59	UTSW	7	41,693,177 (GRCm39)	missense	probably benign	0.02
R6690:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6768:Vmn2r59	UTSW	7	41,661,392 (GRCm39)	missense	probably benign	0.17
R6830:Vmn2r59	UTSW	7	41,693,171 (GRCm39)	missense	probably benign	0.10
R6859:Vmn2r59	UTSW	7	41,693,277 (GRCm39)	missense	probably damaging	1.00
R7034:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7036:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7145:Vmn2r59	UTSW	7	41,695,188 (GRCm39)	missense	probably damaging	1.00
R7556:Vmn2r59	UTSW	7	41,695,233 (GRCm39)	missense	probably damaging	1.00
R7733:Vmn2r59	UTSW	7	41,661,443 (GRCm39)	missense	probably benign	0.17
R7770:Vmn2r59	UTSW	7	41,708,336 (GRCm39)	missense	probably damaging	1.00
R7812:Vmn2r59	UTSW	7	41,695,196 (GRCm39)	nonsense	probably null
R7867:Vmn2r59	UTSW	7	41,661,707 (GRCm39)	missense	probably damaging	1.00
R7975:Vmn2r59	UTSW	7	41,693,199 (GRCm39)	missense	probably damaging	1.00
R7999:Vmn2r59	UTSW	7	41,696,256 (GRCm39)	missense	probably damaging	1.00
R8267:Vmn2r59	UTSW	7	41,661,521 (GRCm39)	missense	probably damaging	0.97
R8367:Vmn2r59	UTSW	7	41,661,247 (GRCm39)	missense	probably benign	0.44
R9106:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R9135:Vmn2r59	UTSW	7	41,693,127 (GRCm39)	missense
R9135:Vmn2r59	UTSW	7	41,693,125 (GRCm39)	missense	probably benign	0.33
R9234:Vmn2r59	UTSW	7	41,661,907 (GRCm39)	missense	possibly damaging	0.67
R9273:Vmn2r59	UTSW	7	41,695,286 (GRCm39)	nonsense	probably null
R9432:Vmn2r59	UTSW	7	41,696,254 (GRCm39)	missense	probably damaging	1.00
R9433:Vmn2r59	UTSW	7	41,695,590 (GRCm39)	missense	probably damaging	0.99
R9616:Vmn2r59	UTSW	7	41,661,299 (GRCm39)	missense	probably damaging	1.00
R9654:Vmn2r59	UTSW	7	41,693,217 (GRCm39)	missense	probably benign	0.10
R9741:Vmn2r59	UTSW	7	41,708,209 (GRCm39)	missense	probably damaging	0.99
X0025:Vmn2r59	UTSW	7	41,695,365 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r59	UTSW	7	41,661,838 (GRCm39)	missense	possibly damaging	0.85
Z1176:Vmn2r59	UTSW	7	41,691,941 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCCTTTGTGCTATGGTAGACAG -3'
(R):5'- TCTCCACAGTGTTGGCCAAAG -3'

Sequencing Primer
(F):5'- CACAGAAGAATACCTGTATGCTG -3'
(R):5'- GTTGGCCAAAGCTATTACAGTCG -3'

Posted On

2015-06-24