Mutagenetix > Incidental Mutation

Incidental Mutation 'R4275:Zfp651'

324761

Institutional Source

Beutler Lab

Gene Symbol

Zfp651

Ensembl Gene

ENSMUSG00000013419

Gene Name

zinc finger protein 651

Synonyms

4732420M22Rik

MMRRC Submission

041646-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R4275 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

121759330-121771742 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 121766539 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 576 (V576D)

Ref Sequence

ENSEMBL: ENSMUSP00000091286 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000093772]

AlphaFold

E9PZ11

Predicted Effect

probably damaging
Transcript: ENSMUST00000093772
AA Change: V576D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000091286
Gene: ENSMUSG00000013419
AA Change: V576D

Domain	Start	End	E-Value	Type
BTB	45	141	3.69e-19	SMART
low complexity region	159	164	N/A	INTRINSIC
low complexity region	197	210	N/A	INTRINSIC
low complexity region	217	231	N/A	INTRINSIC
low complexity region	302	339	N/A	INTRINSIC
low complexity region	355	372	N/A	INTRINSIC
ZnF_C2H2	447	470	1.28e-3	SMART
ZnF_C2H2	474	494	8.4e1	SMART
ZnF_C2H2	501	524	1.76e-1	SMART
ZnF_C2H2	531	553	3.34e-2	SMART
ZnF_C2H2	559	581	6.78e-3	SMART
ZnF_C2H2	587	609	3.63e-3	SMART
ZnF_C2H2	615	637	1.95e-3	SMART
ZnF_C2H2	643	665	6.62e-6	SMART
ZnF_C2H2	671	698	4.16e1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000213743

Predicted Effect

probably benign
Transcript: ENSMUST00000214732

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000215081

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.4%
20x: 95.7%

Validation Efficiency

98% (43/44)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Cacna1e	T	C	1: 154,493,325	Y322C	probably damaging	Het
Camsap2	A	G	1: 136,270,876	V1462A	probably benign	Het
D3Ertd751e	C	A	3: 41,756,154		probably benign	Het
Dhcr7	C	T	7: 143,843,227	A152V	probably damaging	Het
Enpep	A	T	3: 129,332,278	N68K	probably benign	Het
Fam126b	T	C	1: 58,529,933	T440A	probably benign	Het
Fam131b	G	A	6: 42,321,307	L43F	probably damaging	Het
Fbxl5	T	A	5: 43,762,772		probably benign	Het
Hspg2	C	T	4: 137,518,940	R1010C	probably damaging	Het
Igf2	T	C	7: 142,655,786	M46V	probably benign	Het
Kntc1	T	A	5: 123,767,779	Y367N	probably damaging	Het
Mapk8ip2	T	C	15: 89,458,995	W647R	probably damaging	Het
Mettl21c	C	T	1: 44,010,556	V110I	probably damaging	Het
Mrgprh	T	C	17: 12,877,227	L118P	probably damaging	Het
Myadm	A	G	7: 3,297,102	T127A	probably benign	Het
Myh10	A	T	11: 68,751,940		probably null	Het
Nadk	T	A	4: 155,584,255	Y128N	probably benign	Het
Olfr1089	T	C	2: 86,733,592	T7A	probably damaging	Het
Olfr996	T	C	2: 85,579,863	V208A	probably benign	Het
Papolg	A	G	11: 23,868,378	I500T	probably benign	Het
Pkhd1	A	T	1: 20,058,384	C4032S	probably benign	Het
Rnase1	A	T	14: 51,145,870	L9Q	probably damaging	Het
Rspry1	G	T	8: 94,649,761	V304L	probably benign	Het
Sall2	C	A	14: 52,313,803	R643L	probably damaging	Het
Scpep1	T	C	11: 88,947,142		probably null	Het
Serpina3m	T	A	12: 104,389,116	I14N	probably damaging	Het
Smg6	A	C	11: 74,993,874		probably benign	Het
Suz12	T	C	11: 80,030,053	M593T	probably damaging	Het
Tmem139	A	G	6: 42,264,105	E208G	probably damaging	Het
Tnxb	A	G	17: 34,698,231	Y2200C	probably damaging	Het
Usp19	T	G	9: 108,498,694	V911G	probably damaging	Het
Vipr1	T	C	9: 121,664,618	L245P	probably damaging	Het
Vmn2r105	T	A	17: 20,228,640	I92F	probably damaging	Het
Zfp518b	A	G	5: 38,671,728	V978A	probably damaging	Het

Other mutations in Zfp651

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01913:Zfp651	APN	9	121763969	missense	probably damaging	1.00
R0193:Zfp651	UTSW	9	121767666	missense	probably damaging	0.98
R0270:Zfp651	UTSW	9	121767575	missense	probably benign	0.27
R0347:Zfp651	UTSW	9	121763102	missense	probably damaging	1.00
R0654:Zfp651	UTSW	9	121763261	missense	probably benign	0.01
R2202:Zfp651	UTSW	9	121762637	missense	possibly damaging	0.53
R2203:Zfp651	UTSW	9	121762637	missense	possibly damaging	0.53
R2204:Zfp651	UTSW	9	121762637	missense	possibly damaging	0.53
R2205:Zfp651	UTSW	9	121762637	missense	possibly damaging	0.53
R2364:Zfp651	UTSW	9	121767594	missense	probably damaging	0.98
R3843:Zfp651	UTSW	9	121763433	missense	possibly damaging	0.86
R4934:Zfp651	UTSW	9	121763979	missense	probably damaging	0.99
R5358:Zfp651	UTSW	9	121765595	missense	probably damaging	1.00
R5462:Zfp651	UTSW	9	121767663	missense	probably damaging	0.99
R5613:Zfp651	UTSW	9	121767519	missense	probably damaging	1.00
R5843:Zfp651	UTSW	9	121767339	missense	possibly damaging	0.47
R5863:Zfp651	UTSW	9	121767530	missense	probably benign	0.22
R6009:Zfp651	UTSW	9	121762871	missense	possibly damaging	0.86
R6063:Zfp651	UTSW	9	121763532	missense	probably benign	0.01
R6114:Zfp651	UTSW	9	121765595	missense	probably damaging	1.00
R6223:Zfp651	UTSW	9	121763787	missense	possibly damaging	0.61
R6414:Zfp651	UTSW	9	121763659	missense	probably benign
R6811:Zfp651	UTSW	9	121766529	missense	probably damaging	1.00
R7394:Zfp651	UTSW	9	121767345	missense	probably damaging	0.98
R7430:Zfp651	UTSW	9	121763666	missense	probably benign	0.02
R8215:Zfp651	UTSW	9	121767278	missense	probably benign	0.40
R8900:Zfp651	UTSW	9	121767639	missense	probably damaging	0.99
R8982:Zfp651	UTSW	9	121763268	missense	probably benign	0.26
R9623:Zfp651	UTSW	9	121762924	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TCTAGAAAAGCGTGGGGTGC -3'
(R):5'- AGGCCTGCAAAGAAACATTTCC -3'

Sequencing Primer
(F):5'- CCTGAAGAAGTGACGTCT -3'
(R):5'- GAATAGAAGCCCTGCTCCTCCTG -3'

Posted On

2015-06-24