|Institutional Source||Beutler Lab|
|Gene Name||serine/threonine kinase 4|
|Synonyms||Ysk3, sterile 20-like kinase 1, Kas-2, Mst1|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R4360 (G1)|
|Chromosomal Location||164070322-164155524 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 164088959 bp|
|Amino Acid Change||Glutamic Acid to Glycine at position 160 (E160G)|
|Ref Sequence||ENSEMBL: ENSMUSP00000018353 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000018353] [ENSMUST00000134078]|
|Predicted Effect||possibly damaging
AA Change: E160G
PolyPhen 2 Score 0.939 (Sensitivity: 0.80; Specificity: 0.94)
AA Change: E160G
|Predicted Effect||probably benign
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.7825|
|Coding Region Coverage||
|Validation Efficiency||100% (50/50)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a gene trap allele have low numbers of naï¿½ve T cells that are hyper-responsive to stimulation. Mice homozygous for knock-out alleles exhibit decreased peripheral T cell numbers due to impaired emigration and homing. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Stk4||
(F):5'- TGAAGCAGCTGTGTGTCAC -3'
(R):5'- TGAGTCAACAGGCATGCATG -3'
(F):5'- TCACGGAGTTTGGATGTAGC -3'
(R):5'- TGCATGCGCACACACACG -3'