Mutagenetix > Incidental Mutations

Incidental Mutation 'R0010:Olfr835'

32486

Institutional Source

Beutler Lab

Gene Symbol

Olfr835

Ensembl Gene

ENSMUSG00000045204

Gene Name

olfactory receptor 835

Synonyms

MOR150-3, GA_x6K02T2PVTD-12771995-12772930

MMRRC Submission

038305-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R0010 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

19032735-19037670 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 19035322 bp

Zygosity

Heterozygous

Amino Acid Change

Leucine to Phenylalanine at position 66 (L66F)

Ref Sequence

ENSEMBL: ENSMUSP00000148416 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059315] [ENSMUST00000213018]

Predicted Effect

probably damaging
Transcript: ENSMUST00000059315
AA Change: L66F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000052610
Gene: ENSMUSG00000045204
AA Change: L66F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.8e-47	PFAM
Pfam:7TM_GPCR_Srsx	35	206	2.9e-7	PFAM
Pfam:7tm_1	41	303	1.3e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213018
AA Change: L66F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.0%
20x: 94.9%

Validation Efficiency

100% (89/89)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 82 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrf3	T	C	5: 30,205,609		probably benign	Het
Ahrr	G	A	13: 74,283,024		probably benign	Het
Bbs7	T	C	3: 36,607,717		probably null	Het
BC037034	T	C	5: 138,260,293		probably null	Het
Cacna1h	T	C	17: 25,380,844	K1566E	probably damaging	Het
Ccdc73	C	T	2: 104,980,987		probably benign	Het
Cd74	A	T	18: 60,809,071	H124L	probably benign	Het
Cd74	A	T	18: 60,803,896		probably benign	Het
Cdk5rap2	T	C	4: 70,243,459	E270G	probably benign	Het
Ces2a	G	A	8: 104,741,396	D520N	probably benign	Het
Cldnd1	T	A	16: 58,731,259		probably benign	Het
Cox17	T	A	16: 38,347,170	C24S	possibly damaging	Het
Cyp2b9	T	A	7: 26,186,753		probably benign	Het
Dennd4a	T	C	9: 64,896,715	L1112P	probably benign	Het
Dennd4c	T	C	4: 86,781,577	S222P	probably damaging	Het
Dhx37	T	A	5: 125,431,616	Q85L	probably benign	Het
Egfem1	G	T	3: 29,582,919	C192F	probably damaging	Het
Eif3f	A	T	7: 108,941,005	N336Y	possibly damaging	Het
Evc2	T	A	5: 37,417,449	L1016Q	probably damaging	Het
Fam114a2	G	T	11: 57,514,156	T40N	probably damaging	Het
Fam135b	T	C	15: 71,622,032	K16R	probably damaging	Het
Fcho1	A	G	8: 71,709,999	Y725H	probably damaging	Het
Frem1	T	C	4: 83,000,098	I536V	probably benign	Het
Ginm1	T	C	10: 7,775,374		probably benign	Het
Glrb	A	T	3: 80,860,315		probably benign	Het
Glt6d1	C	A	2: 25,794,727		probably null	Het
Gm10320	T	C	13: 98,489,546	Y110C	probably damaging	Het
Gm20388	G	A	8: 122,270,598		probably benign	Het
Gm3985	A	T	8: 32,942,456		noncoding transcript	Het
Gm5422	A	G	10: 31,249,754		noncoding transcript	Het
Igkv6-29	A	T	6: 70,138,770		probably benign	Het
Inpp5d	G	A	1: 87,697,546		probably null	Het
Itpr3	T	G	17: 27,120,977	V2610G	probably damaging	Het
Kmt5c	T	A	7: 4,746,208	M88K	probably benign	Het
Lrp12	C	T	15: 39,878,276	A367T	probably damaging	Het
Ltbp1	A	G	17: 75,363,391	T1476A	probably damaging	Het
Mcoln2	C	T	3: 146,183,561	T374M	probably damaging	Het
Milr1	T	G	11: 106,767,003	*209G	probably null	Het
Mitf	A	G	6: 97,807,281	K33R	probably benign	Het
Mon2	A	C	10: 123,032,694	S485A	probably damaging	Het
Mpdu1	C	T	11: 69,658,841	G47R	probably damaging	Het
Ms4a4d	A	G	19: 11,554,826	N112S	probably damaging	Het
Mybpc3	G	A	2: 91,134,833	W1082*	probably null	Het
Myl3	A	C	9: 110,767,929	D119A	probably damaging	Het
Naa15	A	T	3: 51,436,213		probably null	Het
Nav3	A	G	10: 109,823,226		probably benign	Het
Nek7	T	A	1: 138,544,204	Q66L	possibly damaging	Het
Nktr	G	A	9: 121,741,166		probably benign	Het
Nlgn1	G	T	3: 25,435,842		probably benign	Het
Npr1	T	C	3: 90,454,832	E1002G	probably damaging	Het
Nup133	A	T	8: 123,904,579	I1072N	probably damaging	Het
Oc90	C	T	15: 65,876,548	C371Y	probably damaging	Het
Olfr901	A	T	9: 38,430,920	I213F	possibly damaging	Het
Olfr994	A	T	2: 85,429,895	D311E	probably benign	Het
Pradc1	A	T	6: 85,447,231	N44K	probably damaging	Het
Pradc1	T	C	6: 85,447,620	D116G	probably damaging	Het
Ptprk	G	A	10: 28,585,969	C91Y	probably damaging	Het
Pus7	T	C	5: 23,747,845	I491V	probably benign	Het
Rock1	T	A	18: 10,084,380	D951V	probably damaging	Het
Scgb2b26	T	A	7: 33,944,349	E55D	probably damaging	Het
Scn8a	T	C	15: 101,013,573	V958A	probably damaging	Het
Sec14l1	T	C	11: 117,143,770		probably benign	Het
Sec24c	A	G	14: 20,689,261		probably benign	Het
Sema6b	C	T	17: 56,124,105	E853K	probably benign	Het
Sgk1	G	A	10: 21,997,438		probably null	Het
Shprh	C	T	10: 11,151,931	T94I	probably benign	Het
Slc16a3	T	C	11: 120,956,705	S240P	probably benign	Het
Slc5a8	T	C	10: 88,886,590	V95A	probably benign	Het
Smg1	A	T	7: 118,171,859		probably benign	Het
Spta1	G	A	1: 174,217,943	V1556I	probably benign	Het
Svs1	A	T	6: 48,988,906	H616L	probably damaging	Het
Trappc4	G	A	9: 44,405,231		probably benign	Het
Tubgcp6	A	G	15: 89,103,183	S1188P	probably benign	Het
Txlna	T	G	4: 129,629,086	D487A	probably benign	Het
Ube2d2b	T	C	5: 107,830,636	F51S	possibly damaging	Het
Vmn2r6	G	A	3: 64,559,545	Q178*	probably null	Het
Wdfy3	T	C	5: 101,848,349	T3234A	probably damaging	Het
Ylpm1	C	A	12: 85,029,026	Q384K	probably damaging	Het
Zbtb41	T	G	1: 139,423,530	V127G	probably damaging	Het
Zfp605	T	A	5: 110,127,534	C173S	probably benign	Het
Zfp608	A	T	18: 54,895,214		probably benign	Het
Zhx2	T	C	15: 57,821,274	V13A	possibly damaging	Het

Other mutations in Olfr835

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01718:Olfr835	APN	9	19035288	nonsense	probably null
IGL02546:Olfr835	APN	9	19035354	missense	possibly damaging	0.88
R0107:Olfr835	UTSW	9	19035333	missense	probably damaging	1.00
R1867:Olfr835	UTSW	9	19035266	missense	probably benign	0.36
R1891:Olfr835	UTSW	9	19035978	missense	probably damaging	0.99
R1941:Olfr835	UTSW	9	19035866	missense	possibly damaging	0.93
R4152:Olfr835	UTSW	9	19035520	nonsense	probably null
R5451:Olfr835	UTSW	9	19035491	missense	probably damaging	1.00
R5677:Olfr835	UTSW	9	19035558	missense	possibly damaging	0.93
R5970:Olfr835	UTSW	9	19035147	missense	probably benign
R6187:Olfr835	UTSW	9	19035393	missense	probably benign	0.12
R6805:Olfr835	UTSW	9	19035301	missense	probably damaging	1.00
R7183:Olfr835	UTSW	9	19035332	missense	probably damaging	1.00
R8029:Olfr835	UTSW	9	19035794	missense	probably damaging	1.00
R8139:Olfr835	UTSW	9	19035575	missense	probably benign	0.07
R8736:Olfr835	UTSW	9	19035478	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAACAGATGATCCAACTGTGAAGCCTG -3'
(R):5'- CCATGAGACCAAGAAGTCCCATGTTC -3'

Sequencing Primer
(F):5'- AACTGTGAAGCCTGTGATCTTTTG -3'
(R):5'- GTCCCATGTTCAAAACGCTAATG -3'

Posted On

2013-05-09