Mutagenetix > Incidental Mutation

Incidental Mutation 'R4389:Rpl7a-ps3'

326342

Institutional Source

Beutler Lab

Gene Symbol

Rpl7a-ps3

Ensembl Gene

ENSMUSG00000068579

Gene Name

ribosomal protein L7A, pseudogene 3

Synonyms

Gm5044

MMRRC Submission

041126-MU

Accession Numbers

Essential gene?

Not available question?

Stock #

R4389 (G1)

Quality Score

120

Status

Not validated

Chromosome

Chromosomal Location

36308295-36309090 bp(-) (GRCm39)

Type of Mutation

exon

DNA Base Change (assembly)

G to A at 36308429 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000087632 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090170]

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000090170

SMART Domains

Protein: ENSMUSP00000087632
Gene: ENSMUSG00000068579

Domain	Start	End	E-Value	Type
low complexity region	4	32	N/A	INTRINSIC
Pfam:Ribosomal_L7Ae	126	220	1.6e-25	PFAM

Coding Region Coverage

1x: 99.6%
3x: 98.7%
10x: 96.6%
20x: 92.6%

Validation Efficiency

87% (26/30)

Allele List at MGI

Other mutations in this stock

Total: 22 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	C	11: 9,247,878 (GRCm39)	T2542P	probably damaging	Het
Adprm	C	T	11: 66,929,019 (GRCm39)	R324K	probably benign	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,619,782 (GRCm39)		probably benign	Het
Cd209b	A	G	8: 3,975,960 (GRCm39)	L67P	probably damaging	Het
Cfap54	T	A	10: 92,803,362 (GRCm39)	K1560M	probably benign	Het
Ctps1	T	C	4: 120,415,987 (GRCm39)	D212G	probably damaging	Het
Ercc6	T	A	14: 32,296,865 (GRCm39)	L1285*	probably null	Het
Gzma	G	T	13: 113,234,922 (GRCm39)		probably null	Het
Kng2	T	C	16: 22,843,618 (GRCm39)	I120M	possibly damaging	Het
Lhx3	C	T	2: 26,091,102 (GRCm39)		probably benign	Het
Mtfr1l	T	C	4: 134,259,953 (GRCm39)		probably benign	Het
Ndufb4	T	C	16: 37,468,032 (GRCm39)	N126S	probably benign	Het
Nlrp4e	A	G	7: 23,020,652 (GRCm39)	I380V	probably benign	Het
Nptn	A	G	9: 58,551,055 (GRCm39)	K361E	probably damaging	Het
Or5an1c	A	G	19: 12,218,503 (GRCm39)	V174A	probably damaging	Het
Orc2	T	C	1: 58,514,020 (GRCm39)	D332G	probably benign	Het
Pcdha4	T	C	18: 37,087,842 (GRCm39)	V675A	probably benign	Het
Slc13a1	A	T	6: 24,092,397 (GRCm39)		probably null	Het
Tec	A	G	5: 72,939,350 (GRCm39)	Y222H	probably benign	Het
Ttll2	T	A	17: 7,618,599 (GRCm39)	R443*	probably null	Het
Vmn1r66	A	T	7: 10,008,715 (GRCm39)	L106*	probably null	Het
Zfp189	A	G	4: 49,529,934 (GRCm39)	R346G	probably damaging	Het

Other mutations in Rpl7a-ps3

Allele	Source	Chr	Coord	Type	Predicted Effect
R1659:Rpl7a-ps3	UTSW	15	36,308,309 (GRCm39)	exon	noncoding transcript
R3623:Rpl7a-ps3	UTSW	15	36,308,429 (GRCm39)	exon	noncoding transcript
R4350:Rpl7a-ps3	UTSW	15	36,308,283 (GRCm39)	unclassified	noncoding transcript
R4387:Rpl7a-ps3	UTSW	15	36,308,429 (GRCm39)	exon	noncoding transcript
R4486:Rpl7a-ps3	UTSW	15	36,308,429 (GRCm39)	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- GGCAACTTTCTTATGAAGTTCAGTG -3'
(R):5'- TAGACCCCATTGAGCTGGTG -3'

Sequencing Primer
(F):5'- TCAGTGTGTGTCCAGCAGC -3'
(R):5'- CCATTGAGCTGGTGGTTTTCCTAC -3'

Posted On

2015-07-06