Mutagenetix > Incidental Mutation

Incidental Mutation 'R4424:Mapk11'

327286

Institutional Source

Beutler Lab

Gene Symbol

Mapk11

Ensembl Gene

ENSMUSG00000053137

Gene Name

mitogen-activated protein kinase 11

Synonyms

p38beta, Sapk2, Prkm11, P38b

MMRRC Submission

041696-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4424 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

89026685-89033809 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 89029576 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000086204 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088823] [ENSMUST00000088827]

AlphaFold

Q9WUI1

Predicted Effect

probably null
Transcript: ENSMUST00000088823

SMART Domains

Protein: ENSMUSP00000086204
Gene: ENSMUSG00000053137

Domain	Start	End	E-Value	Type
S_TKc	24	308	1.67e-84	SMART
low complexity region	313	325	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000088827

SMART Domains

Protein: ENSMUSP00000086207
Gene: ENSMUSG00000022610

Domain	Start	End	E-Value	Type
S_TKc	27	311	1.63e-96	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000228967

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000229193

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000230266

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000230634

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000230734

Meta Mutation Damage Score

0.9594

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.3%

Validation Efficiency

95% (69/73)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of a family of protein kinases that are involved in the integration of biochemical signals for a wide variety of cellular processes, including cell proliferation, differentiation, transcriptional regulation, and development. The encoded protein can be activated by proinflammatory cytokines and environmental stresses through phosphorylation by mitogen activated protein kinase kinases (MKKs). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit a normal phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933409G03Rik	A	T	2: 68,445,491 (GRCm39)		probably benign	Het
Aimp1	A	T	3: 132,373,253 (GRCm39)	L229Q	probably benign	Het
Ankrd16	T	A	2: 11,789,215 (GRCm39)	D267E	possibly damaging	Het
Apol11b	A	G	15: 77,522,133 (GRCm39)		probably null	Het
Arfgap1	C	A	2: 180,622,869 (GRCm39)	D327E	probably benign	Het
Arid3b	A	T	9: 57,741,151 (GRCm39)	D98E	probably benign	Het
Art2b	T	A	7: 101,229,129 (GRCm39)	I257F	probably benign	Het
Atp5f1a	T	A	18: 77,867,766 (GRCm39)		probably benign	Het
Carmil3	A	G	14: 55,738,928 (GRCm39)	T861A	probably benign	Het
Cep164	A	T	9: 45,691,002 (GRCm39)	F1259L	possibly damaging	Het
Cfap91	G	T	16: 38,140,727 (GRCm39)	P409T	probably damaging	Het
Chrnd	T	C	1: 87,123,512 (GRCm39)	V350A	probably benign	Het
Clcn7	T	C	17: 25,379,150 (GRCm39)	L744P	probably damaging	Het
Csl	T	A	10: 99,594,453 (GRCm39)	D204V	possibly damaging	Het
Cstdc3	A	G	16: 36,132,951 (GRCm39)	D76G	probably null	Het
Cyp2c29	G	T	19: 39,275,620 (GRCm39)	W20L	probably damaging	Het
Dhrs13	G	T	11: 77,927,951 (GRCm39)	G266*	probably null	Het
Dll3	T	C	7: 27,995,716 (GRCm39)	N362D	probably damaging	Het
Efcab3	T	G	11: 104,626,940 (GRCm39)		probably null	Het
Fanca	A	G	8: 124,015,532 (GRCm39)	V715A	probably benign	Het
Fhod1	T	C	8: 106,063,983 (GRCm39)		probably benign	Het
Fpr2	C	T	17: 18,113,394 (GRCm39)	P130L	probably damaging	Het
Glce	A	G	9: 61,967,535 (GRCm39)	Y539H	probably damaging	Het
Hfe	A	T	13: 23,890,866 (GRCm39)	V91E	probably benign	Het
Hoxd13	A	T	2: 74,500,301 (GRCm39)	K281*	probably null	Het
Ighv1-66	A	T	12: 115,557,157 (GRCm39)	W3R	probably damaging	Het
Impg2	G	A	16: 56,080,388 (GRCm39)	V622I	possibly damaging	Het
Jun	A	G	4: 94,939,084 (GRCm39)	M142T	probably benign	Het
Krt78	T	C	15: 101,856,375 (GRCm39)	T479A	probably benign	Het
Lama3	T	A	18: 12,652,929 (GRCm39)	C216*	probably null	Het
Lin54	G	T	5: 100,594,419 (GRCm39)	T582K	probably damaging	Het
Mindy3	A	G	2: 12,353,010 (GRCm39)	M397T	probably benign	Het
Mrpl41	T	C	2: 24,864,418 (GRCm39)	T85A	possibly damaging	Het
Msh6	T	A	17: 88,298,217 (GRCm39)	L1354*	probably null	Het
Mtmr12	T	C	15: 12,230,400 (GRCm39)	V41A	probably damaging	Het
Myh2	A	T	11: 67,083,551 (GRCm39)	Q1478L	probably benign	Het
Myo6	A	G	9: 80,195,320 (GRCm39)	K897E	probably benign	Het
Naprt	G	T	15: 75,764,605 (GRCm39)		probably null	Het
Nrl	G	A	14: 55,759,675 (GRCm39)	S84L	probably benign	Het
Nxf1	A	G	19: 8,744,128 (GRCm39)		probably benign	Het
Or8g2b	A	T	9: 39,751,652 (GRCm39)	R307S	possibly damaging	Het
Panx2	G	T	15: 88,952,423 (GRCm39)	V305F	probably benign	Het
Pcdhga1	T	C	18: 37,795,632 (GRCm39)	L212P	probably damaging	Het
Pik3ap1	G	A	19: 41,364,320 (GRCm39)	T133I	probably benign	Het
Ppat	A	G	5: 77,063,061 (GRCm39)	W517R	probably damaging	Het
Ppp1r16b	C	T	2: 158,599,174 (GRCm39)	T382I	probably benign	Het
Prkdc	G	A	16: 15,653,946 (GRCm39)	R3901H	probably damaging	Het
Prkdc	A	G	16: 15,591,603 (GRCm39)	K2694E	probably damaging	Het
Psma8	A	G	18: 14,854,247 (GRCm39)	I42M	probably damaging	Het
Ptprd	A	T	4: 76,021,200 (GRCm39)	M599K	probably benign	Het
Rnmt	A	G	18: 68,444,742 (GRCm39)	D237G	probably null	Het
Rpl31-ps17	C	T	12: 54,748,397 (GRCm39)		noncoding transcript	Het
Scaf11	G	A	15: 96,316,309 (GRCm39)	T1085I	possibly damaging	Het
Sec14l3	G	A	11: 4,016,210 (GRCm39)	R43Q	probably damaging	Het
Shc4	G	T	2: 125,494,442 (GRCm39)	T131K	probably benign	Het
Snx27	A	G	3: 94,469,330 (GRCm39)	F4L	probably benign	Het
Sorcs1	T	C	19: 50,367,379 (GRCm39)	T228A	probably damaging	Het
Spmap2l	T	C	5: 77,202,383 (GRCm39)	I268T	possibly damaging	Het
Sptan1	C	T	2: 29,919,721 (GRCm39)		probably benign	Het
Syvn1	C	T	19: 6,099,951 (GRCm39)		probably benign	Het
Tex47	G	T	5: 7,355,364 (GRCm39)	A182S	probably benign	Het
Tpcn1	T	C	5: 120,680,583 (GRCm39)	K549R	probably damaging	Het
Upf3a	C	A	8: 13,846,573 (GRCm39)	P318T	probably benign	Het
Zbtb40	A	T	4: 136,726,005 (GRCm39)	M518K	probably damaging	Het
Zcchc14	G	A	8: 122,378,680 (GRCm39)		probably benign	Het
Zfp687	G	A	3: 94,916,439 (GRCm39)	P861L	probably damaging	Het

Other mutations in Mapk11

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01744:Mapk11	APN	15	89,031,046 (GRCm39)	splice site	probably benign
IGL02164:Mapk11	APN	15	89,029,651 (GRCm39)	critical splice acceptor site	probably null
IGL02825:Mapk11	APN	15	89,030,585 (GRCm39)	missense	probably damaging	1.00
E0370:Mapk11	UTSW	15	89,030,716 (GRCm39)	missense	probably damaging	1.00
R1764:Mapk11	UTSW	15	89,028,594 (GRCm39)	critical splice donor site	probably null
R2158:Mapk11	UTSW	15	89,030,575 (GRCm39)	missense	probably damaging	0.98
R3149:Mapk11	UTSW	15	89,029,653 (GRCm39)	splice site	probably null
R3150:Mapk11	UTSW	15	89,029,653 (GRCm39)	splice site	probably null
R3730:Mapk11	UTSW	15	89,029,318 (GRCm39)	missense	probably benign	0.27
R4319:Mapk11	UTSW	15	89,030,946 (GRCm39)	missense	probably damaging	1.00
R4632:Mapk11	UTSW	15	89,030,579 (GRCm39)	missense	probably damaging	1.00
R4783:Mapk11	UTSW	15	89,033,691 (GRCm39)	missense	probably damaging	0.98
R4937:Mapk11	UTSW	15	89,030,685 (GRCm39)	missense	probably benign
R5422:Mapk11	UTSW	15	89,030,488 (GRCm39)	missense	probably damaging	1.00
R5511:Mapk11	UTSW	15	89,029,380 (GRCm39)	critical splice acceptor site	probably null
R5914:Mapk11	UTSW	15	89,030,038 (GRCm39)	missense	probably benign	0.21
R5972:Mapk11	UTSW	15	89,028,387 (GRCm39)	missense	probably benign	0.34
R7290:Mapk11	UTSW	15	89,028,511 (GRCm39)	missense	probably damaging	1.00
R8244:Mapk11	UTSW	15	89,030,007 (GRCm39)	missense	possibly damaging	0.73
R8974:Mapk11	UTSW	15	89,028,014 (GRCm39)	missense	probably benign	0.00
R9276:Mapk11	UTSW	15	89,029,372 (GRCm39)	missense	probably damaging	1.00
R9432:Mapk11	UTSW	15	89,028,631 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTTTGCCAGAACCTCAGGAC -3'
(R):5'- TTATGCCTGAGGGTCACTGG -3'

Sequencing Primer
(F):5'- CAGCTGGTCGATGTCTACCTAG -3'
(R):5'- TCACTGGGCTGGCTCCTC -3'

Posted On

2015-07-07