Mutagenetix > Incidental Mutation

Incidental Mutation 'R4355:Tas2r109'

327484

Institutional Source

Beutler Lab

Gene Symbol

Tas2r109

Ensembl Gene

ENSMUSG00000062528

Gene Name

taste receptor, type 2, member 109

Synonyms

mt2r62, T2R09, Tas2r9, mGR09

MMRRC Submission

041108-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R4355 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132980015-132980965 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 132980181 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 262 (I262N)

Ref Sequence

ENSEMBL: ENSMUSP00000069300 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000067539]

AlphaFold

Q7M707

Predicted Effect

probably benign
Transcript: ENSMUST00000067539
AA Change: I262N

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000069300
Gene: ENSMUSG00000062528
AA Change: I262N

Domain	Start	End	E-Value	Type
Pfam:TAS2R	9	309	4.3e-81	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 94.9%

Validation Efficiency

100% (57/57)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam26a	T	G	8: 43,570,185	Q89H	probably benign	Het
Adgrb1	T	C	15: 74,543,662	F697S	probably damaging	Het
Adgrl2	A	T	3: 148,839,152	V769E	probably damaging	Het
Aldh7a1	A	T	18: 56,548,494	F173L	probably null	Het
Arntl	A	G	7: 113,303,406	I421V	possibly damaging	Het
Bcl3	G	T	7: 19,811,580	C208*	probably null	Het
Casz1	T	C	4: 148,952,335	S1685P	unknown	Het
Cep250	A	G	2: 155,991,525	E1789G	probably damaging	Het
Cep76	A	T	18: 67,626,640	D334E	probably benign	Het
Clca3b	A	T	3: 144,825,458		probably null	Het
Col9a3	A	G	2: 180,606,478	S208G	probably benign	Het
Ddx47	T	C	6: 135,021,505	V388A	probably benign	Het
Dync1h1	C	T	12: 110,632,899	A1896V	possibly damaging	Het
Eif2ak2	T	A	17: 78,858,534	R411S	probably benign	Het
F7	A	G	8: 13,034,774	T267A	probably benign	Het
Fras1	C	A	5: 96,700,242	D1770E	probably benign	Het
G2e3	T	A	12: 51,365,337	Y387N	probably benign	Het
Hspg2	C	T	4: 137,529,418	L1491F	probably damaging	Het
Ighv3-4	T	C	12: 114,253,640	I110M	probably benign	Het
Itgb5	T	A	16: 33,844,997	C28S	probably damaging	Het
Kbtbd11	C	A	8: 15,028,578	N392K	probably damaging	Het
Kcnmb4	A	G	10: 116,473,284	S80P	possibly damaging	Het
Kif21a	C	T	15: 90,970,833	C721Y	probably benign	Het
Kirrel	C	T	3: 87,089,151	M380I	probably null	Het
Klrc3	T	C	6: 129,639,162	M189V	probably benign	Het
Macf1	T	A	4: 123,475,091	E394V	possibly damaging	Het
Mrgprb4	C	T	7: 48,198,701	G160R	possibly damaging	Het
Myb	A	G	10: 21,152,617	S116P	probably damaging	Het
Nf2	G	A	11: 4,780,613	Q513*	probably null	Het
Nmnat3	C	T	9: 98,410,152	T150M	possibly damaging	Het
Olfr361	C	A	2: 37,084,930	V273F	probably benign	Het
Olfr743	T	C	14: 50,533,759	C116R	possibly damaging	Het
Patj	T	G	4: 98,650,454	C210W	possibly damaging	Het
Pde3b	A	G	7: 114,416,287	H246R	probably benign	Het
Pnp2	A	G	14: 50,959,625	H56R	probably benign	Het
Prkg1	A	G	19: 30,569,229		probably benign	Het
Rhbdf1	C	T	11: 32,216,236	S8N	probably damaging	Het
Rimbp3	A	G	16: 17,209,692	K327E	possibly damaging	Het
Rnf219	A	G	14: 104,479,257	V560A	probably benign	Het
Rsph6a	T	A	7: 19,067,078		probably null	Het
Ryr2	T	C	13: 11,649,812	N3535S	probably benign	Het
Ssfa2	A	G	2: 79,641,998	N132S	probably benign	Het
Ston1	T	G	17: 88,637,008	V614G	probably damaging	Het
Svep1	T	A	4: 58,138,695	T466S	possibly damaging	Het
Tmtc1	T	C	6: 148,355,098		probably benign	Het
Tshz2	G	A	2: 169,884,938	E16K	possibly damaging	Het
Ufsp2	T	A	8: 45,985,465	S193R	possibly damaging	Het
Ugt2b5	C	A	5: 87,139,763	E182*	probably null	Het
Usp43	A	G	11: 67,891,464	V376A	probably benign	Het
Utp15	A	T	13: 98,259,247	F76I	possibly damaging	Het
Wdr62	A	C	7: 30,242,248	L1141R	probably damaging	Het
Zfp418	T	A	7: 7,172,162	M18K	probably benign	Het

Other mutations in Tas2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00467:Tas2r109	APN	6	132980023	missense	probably benign	0.04
IGL01837:Tas2r109	APN	6	132980514	missense	probably benign	0.27
IGL02094:Tas2r109	APN	6	132980239	missense	possibly damaging	0.78
R0788:Tas2r109	UTSW	6	132980301	missense	probably benign	0.01
R0849:Tas2r109	UTSW	6	132980893	missense	probably benign	0.00
R1542:Tas2r109	UTSW	6	132980910	missense	possibly damaging	0.93
R1583:Tas2r109	UTSW	6	132980426	missense	probably benign	0.01
R2035:Tas2r109	UTSW	6	132980460	missense	probably benign
R3845:Tas2r109	UTSW	6	132980803	missense	probably damaging	0.99
R4060:Tas2r109	UTSW	6	132980185	missense	probably damaging	1.00
R5353:Tas2r109	UTSW	6	132980631	missense	possibly damaging	0.61
R5860:Tas2r109	UTSW	6	132980701	missense	probably benign	0.06
R6211:Tas2r109	UTSW	6	132980624	nonsense	probably null
R6378:Tas2r109	UTSW	6	132980881	missense	probably benign	0.00
R6861:Tas2r109	UTSW	6	132980085	missense	probably benign	0.43
R7319:Tas2r109	UTSW	6	132980700	missense	probably benign	0.33
R8553:Tas2r109	UTSW	6	132980208	missense	probably benign	0.33
R9447:Tas2r109	UTSW	6	132980307	missense	probably damaging	1.00
Z1088:Tas2r109	UTSW	6	132980301	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- AGATGAAATACATGCAGTCAAATTCT -3'
(R):5'- GAAGTCACATTATTTTCCTGTCTGT -3'

Sequencing Primer
(F):5'- GCTTCCTGTCTCTCAGAA -3'
(R):5'- CGTTGTTTTGTCCCTGTCAAC -3'

Posted On

2015-07-07